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ALPARSDSGCAVPVEFTSAEQAAAHIGANSLQDGPIGRVGLEIEAHCFDLSNPTRRPSWDELSAVIADVP PLPGGSRITVEPGGAVELSGPPYDGPLAAVAALQADRAVLRAEFARRNLGLVLLGTDPLRPTRRVNPGAR YSAMEQFFTASGTAEAGAAMMTATASVQVNLDAGPRDGWAERVRLAHALGPTMIAITANSPMLGGQFTGW CSTRQRVWGQLDSARCGPVLGVDGDDPASEWARYALRAPVMLVNSPDAVPVTNWVPFADWADGRAVLGGR RPTEADLDYHLTTLFPPVRPRRWLEIRYLDSVPDALWPAAVFTLTTLLDDPVAAESAAEATRPVATAWDR AARMGLTDRHLHTAALTCVRLAAERAPAELEESMTLLMRSVQQRRSPADDFSDRVVARGIAAAVRELAKG EL*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_6250 | - | - | 100% (423) | glutamate--cysteine ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3730c | gshA | 1e-158 | 64.54% (423) | glutamate--cysteine ligase gshA (gamma-glutamylcysteine |
| M. gilvum PYR-GCK | Mflv_1315 | - | 1e-178 | 75.31% (409) | glutamate--cysteine ligase, GCS2 |
| M. tuberculosis H37Rv | Rv3704c | gshA | 1e-158 | 64.54% (423) | glutamate--cysteine ligase gshA (gamma-glutamylcysteine |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0369 | - | 1e-109 | 52.02% (396) | glutamate--cysteine ligase GshA |
| M. marinum M | MMAR_5215 | gshA | 1e-164 | 66.27% (421) | glutamate--cysteine ligase GshA |
| M. avium 104 | MAV_0399 | - | 1e-169 | 67.78% (419) | putative glutamate--cysteine ligase |
| M. thermoresistible (build 8) | TH_0772 | gshA | 1e-166 | 68.62% (427) | GLUTAMATE--CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_5486 | - | 1e-175 | 73.65% (406) | glutamate--cysteine ligase, GCS2 |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1315|M.gilvum_PYR-GCK MNLSVLSGRWVKTQRPFHTRRGWLMAHTMASDGVVEHGV---------VP
Mvan_5486|M.vanbaalenii_PYR-1 ------------------------MACTMTSDGVVEQRF---------DP
MSMEG_6250|M.smegmatis_MC2_155 ------------------------MALPARSDSGCAVPV---------E-
TH_0772|M.thermoresistible__bu ------------------------MTLPVTAEAPAT--------------
Mb3730c|M.bovis_AF2122/97 ------------------------MTLAAMTAAASQLDNAAPDD----VE
Rv3704c|M.tuberculosis_H37Rv ------------------------MTLAAMTAAASQLDNAAPDD----VE
MMAR_5215|M.marinum_M ------------------------MTFAPSPAATSQLDSSRAAD----AD
MAV_0399|M.avium_104 ------------------------MTFAPSTAAASRPAGGDPAAELRIAE
MAB_0369|M.abscessus_ATCC_1997 ------------------------MATTATEADTGR-------------T
*: .
Mflv_1315|M.gilvum_PYR-GCK LTSAEDAAQHIVDTCLKDVPPGPVGLEIEAHCYDLSDPFRRPGWDELTAV
Mvan_5486|M.vanbaalenii_PYR-1 LSSAEAAAQHIVDGCLIDGPVGQVGLEVEAHCFDLTDPSRRPGWDELTAT
MSMEG_6250|M.smegmatis_MC2_155 FTSAEQAAAHIGANSLQDGPIGRVGLEIEAHCFDLSNPTRRPSWDELSAV
TH_0772|M.thermoresistible__bu VDSARRAAAHIEASCLRDGPVGRVGLEIEAHCFDVADPGRRPGWAELTDV
Mb3730c|M.bovis_AF2122/97 ITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEV
Rv3704c|M.tuberculosis_H37Rv ITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEV
MMAR_5215|M.marinum_M IAGSAVAAQYIANGCLVDGPIGRVGLEIEAHCYDPADPHRRPSWEEIGDV
MAV_0399|M.avium_104 LTDSAAVAHYIARGCLADAPLGRVGLEVEAHCHDPQDPCRRPGWDEIAAV
MAB_0369|M.abscessus_ATCC_1997 LSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRLREV
. . .* :* .: * * ****:*:* : * ** * .: .
Mflv_1315|M.gilvum_PYR-GCK IADLPRLPGGSLVTVEPGGAVELSGPPATGTADAVSAMVTDRAVLRDVFA
Mvan_5486|M.vanbaalenii_PYR-1 ITALPPLPGGSVVTVEPGGAVELSGPPLAEPASAISAMAADHTVLRAAFA
MSMEG_6250|M.smegmatis_MC2_155 IADVPPLPGGSRITVEPGGAVELSGPPYDGPLAAVAALQADRAVLRAEFA
TH_0772|M.thermoresistible__bu IATVGELPGGSAISVEPGGAVELSGPPLDGPVAAATAMLTDQAVLRAAFA
Mb3730c|M.bovis_AF2122/97 LEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALA
Rv3704c|M.tuberculosis_H37Rv LEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALA
MMAR_5215|M.marinum_M LEGLGKPPGGSAVTVEPGGAVELSGPPCDGILAAIDAMHRDQAVLRSAFA
MAV_0399|M.avium_104 LDALPALPGGSRVTVEPGGAVELSGPPADGVLAAIDAMRRDQAVLRPAFA
MAB_0369|M.abscessus_ATCC_1997 GDSVPDLPGSSAITFEPGGAVELSGPPCSDVWSAISSMRADHEILTAVYR
: **.* ::.************ * :: *: :*
Mflv_1315|M.gilvum_PYR-GCK GAGLGLVLLGADPLRPAGRVNPGSRYAAMERFFAESGTPEAGAAMMTSTA
Mvan_5486|M.vanbaalenii_PYR-1 RAGLGLVLVGADPLRPAARVNPGPRYQAMEAFFRASDSGDAGAAMMTSTA
MSMEG_6250|M.smegmatis_MC2_155 RRNLGLVLLGTDPLRPTRRVNPGARYSAMEQFFTASGTAEAGAAMMTATA
TH_0772|M.thermoresistible__bu RAGYALVMLGADPVRQPQRVNPGSRYRAMETFFAASGTAAAGSAMMTSTA
Mb3730c|M.bovis_AF2122/97 NAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTA
Rv3704c|M.tuberculosis_H37Rv NAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTA
MMAR_5215|M.marinum_M DAGLGLVCLGADPIRPTMRINPGARYQAMEQFFTASQSGMAGAAMMTSTA
MAV_0399|M.avium_104 EAGLGLVFLGADPLRPPQRINPGARYRAMERFFATSRSGEAGAAMMTSTA
MAB_0369|M.abscessus_ATCC_1997 GAGIALASLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMTCTA
. .*. :*:**:* . *:***.** *** .* : .. ***.**
Mflv_1315|M.gilvum_PYR-GCK SVQVNLEAGHRAGWADRVRLAHALGPTMIAIAANSPVLAGKFTGWHSTRQ
Mvan_5486|M.vanbaalenii_PYR-1 SVQVNLEAGPRAGWADRVRLAHALGPTMIAIAANSPVLGGDFTGWQSTRQ
MSMEG_6250|M.smegmatis_MC2_155 SVQVNLDAGPRDGWAERVRLAHALGPTMIAITANSPMLGGQFTGWCSTRQ
TH_0772|M.thermoresistible__bu SIQVNVEAGPRTGWAERIRLAHALGPVMVAIAANSPLLGGRFSGWRSSRQ
Mb3730c|M.bovis_AF2122/97 AIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQ
Rv3704c|M.tuberculosis_H37Rv AIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQ
MMAR_5215|M.marinum_M SIQVNLDAGPQSGWASRVRLAHALGPTMIAIAANSPMLGGEFSGWRSTRQ
MAV_0399|M.avium_104 SIQVNVDAGPREGWAARVRLAHALGPTMIAMTANSPMLDGDFTGWVSSRQ
MAB_0369|M.abscessus_ATCC_1997 SLQVNVQSGTPRQWRDRFVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQ
::***:::* * *. **: :**.* *::*.**:* * :* .:**
Mflv_1315|M.gilvum_PYR-GCK LVWSQLDSARCGPILG-VDGD-DPASDWARYALRAPVMLVHTP---EAVP
Mvan_5486|M.vanbaalenii_PYR-1 LVWSRLDSARCGPILG-ASGD-DPASDWARYALRAPVMLVHSP---DPTP
MSMEG_6250|M.smegmatis_MC2_155 RVWGQLDSARCGPVLG-VDGD-DPASEWARYALRAPVMLVNSP---DAVP
TH_0772|M.thermoresistible__bu RIWGQLDSARCGPILGGRDGVRTPGGDWARYALRAPVMLVNGAGDRTAEP
Mb3730c|M.bovis_AF2122/97 RVWGQMDSARCGPILG-ASGD-HPGIDWAKYALKAPVMMVRSPDTQDTRA
Rv3704c|M.tuberculosis_H37Rv RVWGQMDSARCGPILG-ASGD-HPGIDWAKYALKAPVMMVRSPDTQDTRA
MMAR_5215|M.marinum_M RVWGQMDDGRCGPILG-ASAD-DPATDWAHYALKAPVMLVHNP---DAVA
MAV_0399|M.avium_104 RVWGRMDSARCGPILG-ASGD-DPGTDWARYALKAPVMLVHTP---DAEA
MAB_0369|M.abscessus_ATCC_1997 WIWDNLDPRRCAPVEI----GVDPTESWVEYALRAPVMLVRNKD--GADA
:*..:* **.*: * .*..***:****:*. . .
Mflv_1315|M.gilvum_PYR-GCK VTQWVPFADWADGRVLLDDRRPTLADLDYHLTTLFPPVRPRGFLEVRYLD
Mvan_5486|M.vanbaalenii_PYR-1 VTQWVPFADWADGRVLLDDRPPTLADLDYHLTTLFPPVRPRGFLEVRYLD
MSMEG_6250|M.smegmatis_MC2_155 VTNWVPFADWADGRAVLGGRRPTEADLDYHLTTLFPPVRPRRWLEIRYLD
TH_0772|M.thermoresistible__bu VTDWVPFADWADGRTALGGRRPTVADLEYHLTTLFPPVRPRGWLEIRYLD
Mb3730c|M.bovis_AF2122/97 VTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
Rv3704c|M.tuberculosis_H37Rv VTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
MMAR_5215|M.marinum_M VTHHVPFADWVDGRVLLGDRRPTYADLDYHLTTLFPPVRPRQWLEIRYLD
MAV_0399|M.avium_104 VTRWVPFADWVDGRVLLGGRRPTVADLEYHLTTLFPPVRPRQWLEIRYLD
MAB_0369|M.abscessus_ATCC_1997 VVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRWLELRYLD
*. *** .*.** * .* .* ** ************* :**:****
Mflv_1315|M.gilvum_PYR-GCK SVPDAVWPAVVFTLSTLLDDPVAADVAAEATEPVATSWDRAAQLGLDDRR
Mvan_5486|M.vanbaalenii_PYR-1 SVPDATWPAVVYTLATLLDDPAAADVAAEATEEVATAWDRAAQLGLRDRR
MSMEG_6250|M.smegmatis_MC2_155 SVPDALWPAAVFTLTTLLDDPVAAESAAEATRPVATAWDRAARMGLTDRH
TH_0772|M.thermoresistible__bu CVPDALWPAVVFTLVTLLDDPSAADIAAEATEPVSSAWSRAARDGLADRR
Mb3730c|M.bovis_AF2122/97 SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRR
Rv3704c|M.tuberculosis_H37Rv SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRR
MMAR_5215|M.marinum_M SLSDVFWPAVVFTLVTLLDDPGAAELAFEAVQPVATEWDTAARAGLGDPR
MAV_0399|M.avium_104 SVPDTLWPAVVFTLVALLDDPVAAGLAARAVEPVATAWDVAARVGLRDRR
MAB_0369|M.abscessus_ATCC_1997 AAPDWWWPALAFTAVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPA
. .* *** .:* : **** .* * .. *.. *. **: ** *
Mflv_1315|M.gilvum_PYR-GCK LHTAAVRCVYAAADRAPAALVESMELLTRSVEQGRSPGDDFADRVVNDGI
Mvan_5486|M.vanbaalenii_PYR-1 LHAAATRCVQAAADRAPRALEESMGHLVRSVEQGRSPADDFADRVVAHGI
MSMEG_6250|M.smegmatis_MC2_155 LHTAALTCVRLAAERAPAELEESMTLLMRSVQQRRSPADDFSDRVVARGI
TH_0772|M.thermoresistible__bu LFTAANRCVRTAAERTPAPLLESMERLVRSVERGRCPADDFADLVVRRGI
Mb3730c|M.bovis_AF2122/97 LYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
Rv3704c|M.tuberculosis_H37Rv LYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
MMAR_5215|M.marinum_M LHAAASRCVAIAAERAPAELADALQSLLAYVQRGRCPGDDFGDQVTEHGV
MAV_0399|M.avium_104 LYAAANACLAIAAERAPAELAEPMRRLVRLVEQGRCPGDDFADQVIEHGI
MAB_0369|M.abscessus_ATCC_1997 LHAAAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFIDNVTEYGV
*. ** : *. .* * : * *:: .*.*** * * *:
Mflv_1315|M.gilvum_PYR-GCK AAAVTRLAQGDM---
Mvan_5486|M.vanbaalenii_PYR-1 ADAVTELAQGEL---
MSMEG_6250|M.smegmatis_MC2_155 AAAVRELAKGEL---
TH_0772|M.thermoresistible__bu GDAVDRHGRGDW---
Mb3730c|M.bovis_AF2122/97 ASAVTGMMHGAS---
Rv3704c|M.tuberculosis_H37Rv ASAVTGMMHGAS---
MMAR_5215|M.marinum_M APAIARLARGEQ---
MAV_0399|M.avium_104 AASVSRAARGPQGGP
MAB_0369|M.abscessus_ATCC_1997 EKAFSGAIG------
:.