For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
EHPDPGVDVGHLRAEGVAIIAGSVTDLAGVTRAKYVPVARLGAFARSGMGVSPSWSVFCVDSGIAFTPTI GVAGDLRIRIDPEDLRVVDAGVAWAPGTLNDQHGHPAPLCTRTLLSRVERAAADRGLDVRMGGELECTML SADGGPATTEPWSPYGIRTSLDRSAFLVDLATSAERAGLPLEQLHTEYGHDQLEVSLAPDTPTAAADAVI LARIVLGRVAARHGLRISFSPVPFEGAAGNGAHLHLSLSDADGPLLSGGDGPHGLRTAGAQAIAGVLDTL PDLIGVYAGSAASALRLKPGNWAGATACWGLENREAAVRLIAATPGNPHGANMELKLIDASANPYLAAAA FLGSALRGIGRHLELPEEIPENPAQSGVDTPALPLGQREALDAMETSDVAAELLTPAIVEALVAVRRYES KTFGGLPPAQICDALRLAWTC*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_2920 | - | - | 100% (442) | glutamine synthetase, catalytic region |
| M. vanbaalenii PYR-1 | Mvan_1603 | - | 6e-38 | 32.18% (432) | glutamate--ammonia ligase |
| M. vanbaalenii PYR-1 | Mvan_2278 | - | 7e-26 | 27.15% (372) | glutamate--ammonia ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1910 | glnA3 | 1e-113 | 46.47% (439) | glutamine synthetase |
| M. gilvum PYR-GCK | Mflv_3319 | - | 1e-114 | 47.15% (439) | glutamine synthetase, catalytic region |
| M. tuberculosis H37Rv | Rv1878 | glnA3 | 1e-113 | 46.70% (439) | glutamine synthetase |
| M. leprae Br4923 | MLBr_01631 | glnA2 | 1e-26 | 30.31% (320) | glutamine synthase class II |
| M. abscessus ATCC 19977 | MAB_1933c | - | 2e-24 | 30.35% (257) | glutamine synthetase, type I (GlnA1) |
| M. marinum M | MMAR_2763 | glnA3 | 1e-107 | 45.56% (439) | glutamine synthetase GlnA3 |
| M. avium 104 | MAV_2829 | glnA | 1e-117 | 49.66% (439) | glutamine synthetase catalytic domain |
| M. smegmatis MC2 155 | MSMEG_3561 | glnA | 1e-112 | 47.39% (441) | glutamine synthetase catalytic domain |
| M. thermoresistible (build 8) | TH_3396 | - | 7e-28 | 27.16% (416) | PUTATIVE glutamine synthetase, type I |
| M. ulcerans Agy99 | MUL_1335 | glnA2 | 2e-26 | 28.79% (330) | glutamine synthetase GlnA2 |
CLUSTAL 2.0.9 multiple sequence alignment
Mb1910|M.bovis_AF2122/97 ---MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFA
Rv1878|M.tuberculosis_H37Rv ---MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFA
MAV_2829|M.avium_104 ---MTGTPLAAAAIAQLEAEGVDTVIGTVVNPAGLTLAKTVPIRRTNTFA
MMAR_2763|M.marinum_M MTAMTATPLAAAAIAQLEAEGVDTVIGTVINPAGLALAKTVPVRRTNTFA
Mflv_3319|M.gilvum_PYR-GCK MAGMAAKPLAAAAIAQLEADGVATLIGTVVNPAGLIHAKTVPLRRMGSFA
MSMEG_3561|M.smegmatis_MC2_155 MTSRSGKPLAAAAIAQLESDGVATLIGTVANPAGLIHAKTVPLRRMSTFA
Mvan_2920|M.vanbaalenii_PYR-1 ----MEHPDPGVDVGHLRAEGVAIIAGSVTDLAGVTRAKYVPVARLGAFA
MLBr_01631|M.leprae_Br4923 -----MDRQKEFVLRTLEERDIRFVRLWFTDVLGYLKSVAIAP---AELE
MUL_1335|M.ulcerans_Agy99 -----MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAP---AELE
TH_3396|M.thermoresistible__bu -----MDRQKEFVLRSLEERDIRFVRLWFTDVLGFLKSVAIAP---AELE
MAB_1933c|M.abscessus_ATCC_199 ----MAFSSPDEVFKFIADEKVEYVDVRFCDLPGVVQHFSIPA---SAFD
. : : : . : * :. :
Mb1910|M.bovis_AF2122/97 NPGLGASPVWHTFCIDQCSIAFTADISVVGD-QRLRIDLSALRIIGDGLA
Rv1878|M.tuberculosis_H37Rv NPGLGASPVWHTFCIDQCSIAFTADISVVGD-QRLRIDLSALRIIGDGLA
MAV_2829|M.avium_104 DPGLGASPSWHAFAVDQTGIAFTAEVGVVGD-QRLRIDLSALRIVGDGLA
MMAR_2763|M.marinum_M YPGLGASPTWHGFAVDQSGIAFSDEVGVIGD-QRLRIDLSALRAIGDGLA
Mflv_3319|M.gilvum_PYR-GCK EPGLGASPVWHVFAIDQTGIVFGESTGVVGD-QRIRIDLGALRILGDGFA
MSMEG_3561|M.smegmatis_MC2_155 EPGLGASPVFHAFTIDQVGIVFSDAISVVGD-QRIRIDLSTLRILGDGLA
Mvan_2920|M.vanbaalenii_PYR-1 RSGMGVSPSWSVFCVDS-GIAFTPTIGVAGD-LRIRIDPEDLRVVDAGVA
MLBr_01631|M.leprae_Br4923 G-AFEEGIGFDGSSIEGFARVSESDTVAHPDPSTFQILPWATTAGHHHSA
MUL_1335|M.ulcerans_Agy99 G-AFEEGIGFDGSSIEGFARVSESDTVAHPDPSTFQVLPWVTDAGHHHSA
TH_3396|M.thermoresistible__bu G-AFEEGIGFDGSAIEGFARVSEADMVARPDPSTFQVLPWTGDSGKHYSA
MAB_1933c|M.abscessus_ATCC_199 QSVFDDGLAFDGSSVRGFQSIHESDMLLLPDISTARVDPFRAAKTLNINF
: . : : * ::
Mb1910|M.bovis_AF2122/97 WAPAGFFEQDGTPVPACSRGTLSR--IEAALADAGIDAVIGHEVEFLLVD
Rv1878|M.tuberculosis_H37Rv WAPAGFFEQDGTPVPACSRGTLSR--IEAALADAGIDAVIGHEVEFLLVD
MAV_2829|M.avium_104 WAPAAFFAQDGTPIPSCARGTLKR--VEAALADAGIDAAIGHEVEFVLVA
MMAR_2763|M.marinum_M WAPAAFFEQDGAPVAACTRGTLAR--IEAALEEAGIEAMVGHEIEFLVVN
Mflv_3319|M.gilvum_PYR-GCK WAPGSFYTQDGAPDPYCARGALRR--VSQRLADAGISALVGHEIEFVLVG
MSMEG_3561|M.smegmatis_MC2_155 WAPGAFFDQDGAPDPYCSRYALQR--VADRLETAGLTAQVGHEMEFVLVG
Mvan_2920|M.vanbaalenii_PYR-1 WAPGTLNDQHGHPAPLCTRTLLSR--VERAAADRGLDVRMGGELECTMLS
MLBr_01631|M.leprae_Br4923 RMFCDITMPDGSSSWADPRHVLRR--QLTKANDLGFSCYVHPEIEFFLLK
MUL_1335|M.ulcerans_Agy99 RIFCDITMPDGSPSWADPRHVLRR--QLIKANELGFSCYVHPEIEFFLLN
TH_3396|M.thermoresistible__bu RMFCDITMPDGSPSWADPRHVLRR--QLAKAGDQGFTCYVHPEIEFFLLK
MAB_1933c|M.abscessus_ATCC_199 FVHDPFTREAYS---RDPRNVARKAEQYLASTGIADTCYFGAEAEFYIFD
: .* : . . * * :.
Mb1910|M.bovis_AF2122/97 AD----------------------GQRLPSTL-----------WAQYGVA
Rv1878|M.tuberculosis_H37Rv AD----------------------GQRLPSTL-----------WAQYGVA
MAV_2829|M.avium_104 PD----------------------GQRLPSTL-----------WAQYGLA
MMAR_2763|M.marinum_M PD----------------------GTQRPSTM-----------WAQYGLA
Mflv_3319|M.gilvum_PYR-GCK PD----------------------GARLPAHL-----------WAQYGLA
MSMEG_3561|M.smegmatis_MC2_155 PD----------------------GSRLPSLM-----------WAQYGLA
Mvan_2920|M.vanbaalenii_PYR-1 AD----------------------GGPATTEP-----------WSPYGIR
MLBr_01631|M.leprae_Br4923 AGPEDGSM----------------PIPVPVDN-----------AGYFDQA
MUL_1335|M.ulcerans_Agy99 PGPEDG------------------TEPVPIDN-----------AGYFDQA
TH_3396|M.thermoresistible__bu PGPHDG------------------SPPVPADT-----------SGYFDQS
MAB_1933c|M.abscessus_ATCC_199 SVTFDSKMNGTFYEIDSESGWWNTGEPYEEDGSPNRGYKVRPKGGYFPVA
. . :
Mb1910|M.bovis_AF2122/97 GVLEHEAFVRDVNAAATAAGIAIEQFHPEY-GANQFEISLAPQPPVAAAD
Rv1878|M.tuberculosis_H37Rv GVLEHEAFVRDVNAAATAAGIAIEQFHPEY-GANQFEISLAPQPPVAAAD
MAV_2829|M.avium_104 GVLEHEAFVRDVIGSATRAGIAIEQLHPEY-GANQFELSLAPQPPVAAAD
MMAR_2763|M.marinum_M GVLEYEHFVRDVNASATAAGVGIEQFHPEY-YANQFEISLTPHSPVTAAD
Mflv_3319|M.gilvum_PYR-GCK GVLEHEGFVRDVTAAANASGVAIEQFHPEY-GANQFEISLAPQPPVAAAD
MSMEG_3561|M.smegmatis_MC2_155 GLLEFEGFVREVTDSANASGVAIEQFHPEY-GANQFEISLAPLPPVAAAD
Mvan_2920|M.vanbaalenii_PYR-1 TSLDRSAFLVDLATSAERAGLPLEQLHTEY-GHDQLEVSLAPDTPTAAAD
MLBr_01631|M.leprae_Br4923 VHDSALNFRRHAIEALEFMGISVEFSHHEG-APGQQEIDLRFADALSMAD
MUL_1335|M.ulcerans_Agy99 VHVSASKFRRHVIDALEFMGISVEFSHHEG-APGQQEIDLRFADALSMAD
TH_3396|M.thermoresistible__bu VHDTAPNFRRHAIEALEQMGISVEFSHHEG-APGQQEIDLRYADALSMAD
MAB_1933c|M.abscessus_ATCC_199 PYDHYVDLRDEMSTNLINAGFTLERGHHEVGTAGQAEINYKFDTLLAAAD
: . *. :* * * .* *:. : **
Mb1910|M.bovis_AF2122/97 QLVLTRLIIGRTARRHGLRVSLSPAPFAGSIGSGAHQHFSLTMSEGMLFS
Rv1878|M.tuberculosis_H37Rv QLVLTRLIIGRTARRHGLRVSLSPAPFAGSIGSGAHQHFSLTMSEGMLFS
MAV_2829|M.avium_104 QLILMRLIIGRAARRHGFRISLSPAPFAGDVGSGAHQHFSLSMAEGPLFS
MMAR_2763|M.marinum_M QLVLARIIIGRAARRHGLRVSLSPTPFADNVGCGAHQHFSLSTAEGPMFS
Mflv_3319|M.gilvum_PYR-GCK HVTLMRIIVGRVARTYGMRASLSPVPFAGSVGSGAHQHFSLTRDDVPLFS
MSMEG_3561|M.smegmatis_MC2_155 QLILMRIIVARVARRHGLRASLSPPPFAGGVGSGSHQHFSLCRDDTPLLS
Mvan_2920|M.vanbaalenii_PYR-1 AVILARIVLGRVAARHGLRISFSPVPFEGAAGNGAHLHLSLSDADGPLLS
MLBr_01631|M.leprae_Br4923 NVMTFRYVIKEVAIENGARASFMPKPFAQHPGSAMHTHMSLFEGDVNAFH
MUL_1335|M.ulcerans_Agy99 NVMTFRYVIKEVALEEGVRATFMPKPFGQHAGSAMHTHMSLFEGDVNAFH
TH_3396|M.thermoresistible__bu NVMTFRYVVKQVAISDGVRASFMPKPFAEHPGSAMHTHMSLFEGDTNAFH
MAB_1933c|M.abscessus_ATCC_199 DVLLFKYIIKNTAWANGKTVTFMPKPLFGDNGSGMHAHQSLWKDGKPLFH
: : :: ..* * :: * *: * . * * ** :
Mb1910|M.bovis_AF2122/97 GGTGAAGMTSAGEATVAGVLRGLPDAQGILCGSIVSGLRMRPGNWAGIYA
Rv1878|M.tuberculosis_H37Rv GGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRMRPGNWAGIYA
MAV_2829|M.avium_104 GGAGTAGMTPAGENAVAGVIRGLPEAQGVLCGSIVSGLRMRPGNWAGAYA
MMAR_2763|M.marinum_M EGPGAAGMTTAGEGAVAGVLRGLREAQGILCGSIVSGLRMRPGNWAGAYA
Mflv_3319|M.gilvum_PYR-GCK GGDGPVGMTADGASAVAGVLNGLTEAQGVLSGSVVSGLRMAPGHWSGAYA
MSMEG_3561|M.smegmatis_MC2_155 GGNGARGMTEEGESAVAGLLAGLPAAQGVLCGSVLSGLRVQPGHWAGAHN
Mvan_2920|M.vanbaalenii_PYR-1 GGDGPHGLRTAGAQAIAGVLDTLPDLIGVYAGSAASALRLKPGNWAGATA
MLBr_01631|M.leprae_Br4923 SDDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLVVGGEAPTTA
MUL_1335|M.ulcerans_Agy99 SPDDPLQLSEVGKSFIAGILEHASEISAVTNQWVNSYKRLILGGEAPTAA
TH_3396|M.thermoresistible__bu SPDDPLQLSDVAKSFIAGILEHANEISAVTNQWVNSYKRLVHGGEAPTAA
MAB_1933c|M.abscessus_ATCC_199 DEAGYAGLSDLARHYIGGILHHAPSLLAFTNPTVNSYKRLVPGYEAPINL
. : . :.*:: .. * *: * :
Mb1910|M.bovis_AF2122/97 CWGTENREAAVRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALD
Rv1878|M.tuberculosis_H37Rv CWGTENREAAVRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALD
MAV_2829|M.avium_104 CWGTENREAAVRFIVDGPGSPRGGNVEVKIVDPSANPYLASAAILGLALD
MMAR_2763|M.marinum_M CWGTENREAAVRFVKHVPGAAYGGNVEVKIVDPSANPYLASAAILGLALD
Mflv_3319|M.gilvum_PYR-GCK CWGTENREAAVRYLTRGDANPYGAHVEVKIIDPSANPYLASATILGLALD
MSMEG_3561|M.smegmatis_MC2_155 CWGTENREAAVRFIIGGPSNPHGANVEVKVIDPSANPYLATATILGLALH
Mvan_2920|M.vanbaalenii_PYR-1 CWGLENREAAVRLIAATPGNPHGANMELKLIDASANPYLAAAAFLGSALR
MLBr_01631|M.leprae_Br4923 SWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLAFAVLLAAGLR
MUL_1335|M.ulcerans_Agy99 SWGAANRSALVRVPMYTPHKTSSRRIEVRSPDSACNPYLAFAVLLAAGLR
TH_3396|M.thermoresistible__bu SWGAANRSALVRVPMYTPHKASSRRVEVRSPDSACNPYLTFAVLLAAGLR
MAB_1933c|M.abscessus_ATCC_199 VYSQRNRSACVRIPITG-NNPKAKRLEFRCPDSSGNPYLAFAAMLMAGID
:. **.* ** . . .:*.: *.: ****: *.:* .:
Mb1910|M.bovis_AF2122/97 GMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAIAVLDS---SK
Rv1878|M.tuberculosis_H37Rv GMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAIAVLDS---SK
MAV_2829|M.avium_104 GIKSQAALPPEVTVDPAALSDADRDRDGVVLLTDSAAEAIAALDD---SE
MMAR_2763|M.marinum_M GIQRKVSLPQETVVDPAKLSDTDRDRAGILRLPDDPREVITALDN---SE
Mflv_3319|M.gilvum_PYR-GCK GMTRGLGLPAEVAVDPSSLSQTARAEVGVTVLPTDQTAILDALGA---SA
MSMEG_3561|M.smegmatis_MC2_155 GIDNRLPLPREVSVDPSALTDAQRTESGAELLPCGQAETLPALDA---SP
Mvan_2920|M.vanbaalenii_PYR-1 GIGRHLELPEEIPENP------AQSGVDTPALPLGQREALDAMET---SD
MLBr_01631|M.leprae_Br4923 GVEKGYVLGPQAEDNVWDLTPEERLAMGYRELPTSLDSALGAMEV-SEFV
MUL_1335|M.ulcerans_Agy99 GVEKGYVLGPQAEDNVWDLTPEERRTMGYRELPTSLDSALRAMES-SELV
TH_3396|M.thermoresistible__bu GVEKGYVLGPEAEDNVWTLTPEERRAMGFKELPSSLGQALAEMER-SELV
MAB_1933c|M.abscessus_ATCC_199 GIKNKIEPLAPVDKDLYELPPEE--AAGIPQAPTSLATVIDNLEKDHEYL
*: : . . . : :
Mb1910|M.bovis_AF2122/97 LLRCILGDPVVDAVVAVRQLEHERYGDLDPAQLADKFRMAWSV
Rv1878|M.tuberculosis_H37Rv LLRCILGDPVVDAVVAVRQLEHERYGDLDPAQLADKFRMAWSV
MAV_2829|M.avium_104 LMRHILGDPVVDMVVAVRRLEQERYGDLDPAQLADRFRMAWSL
MMAR_2763|M.marinum_M TMRRVLGDASIDAVVAVRKLMQERYTDLNPEQLANKFRMAWSL
Mflv_3319|M.gilvum_PYR-GCK TMRDILGDAVVDAVIAVRRYEQEHYGGLDPDAVTEKFRLAWSV
MSMEG_3561|M.smegmatis_MC2_155 LIRGILGDEIVDAVLAVRRYEQKNFGELPPEELADRFRLAWSV
Mvan_2920|M.vanbaalenii_PYR-1 VAAELLTPAIVEALVAVRRYESKTFGGLPPAQICDALRLAWTC
MLBr_01631|M.leprae_Br4923 AEA--LGEHVFDFFLRNKRA---EWANYRSHVSLYELKTYLSL
MUL_1335|M.ulcerans_Agy99 AEA--LGEHVFDFFLRNKRR---EWATYRSHVTPYELSTYLSL
TH_3396|M.thermoresistible__bu AEA--LGEHVFDFFLRNKRA---EWENYRSHVTPYELKNYLSL
MAB_1933c|M.abscessus_ATCC_199 TEGGVFTSDLIETWISYKREN--EIEPVNIRPHPYEFALYYDV
: .: : :: :