For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
TAAPVPPIGTQVSALAAQAPDAPAATCDGVTVTRRELDATSNRLARAFASLGVGVGDYVTIVLPNSLDWA YSVLACWKLGAVPQPLSARLPDAELAALLELRRPALLVGRPDPTGVTPDVAVELAAEFPDSPLPEAVSPV WKSMASGGSTGRPKLIEAGNDSRVPPAIGYPLGAQEGDVTLVSVPLSHNTGFTTFAVGLLQGHHLVVMPR FEPHEFLRLVTEHRVTFLTTVPTIMQRLLPVYRADPDAYDLSSIRRFWHLAAPCPPAVKHAWIELLGPDA VWELYGGTELQALTFISGDQWLTHPGSVGVVVAGEMKVLDDDGDECPPGVAGEIYMRSAPGGPPTYRYVG STAKSRDGWESLGDLGYFDEDGFLYLNDRRVDMFTVGGRNVYPAEIESALAEHPRVLSSLAMGLPDDDLG QVPHAIVQAHGLDEAEVIAFLAERIASHKLPRSVEFTDRPLRDDAGKARRSAVRDEVIARRSS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_0252 | - | - | 100% (484) | AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_0218 | - | 4e-65 | 33.86% (511) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0015 | - | 2e-56 | 31.81% (503) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0220 | fadD4 | 1e-59 | 32.26% (499) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0419 | - | 0.0 | 86.13% (483) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0214 | fadD4 | 3e-59 | 32.19% (497) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 7e-49 | 29.90% (495) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2316 | - | 1e-125 | 47.24% (489) | acid-CoA ligase |
| M. marinum M | MMAR_3983 | - | 4e-79 | 38.64% (484) | fatty-acid-CoA ligase |
| M. avium 104 | MAV_1627 | - | 8e-79 | 38.02% (484) | feruloyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_0318 | - | 0.0 | 73.60% (481) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3407 | - | 0.0 | 74.64% (489) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_3844 | - | 8e-78 | 38.02% (484) | fatty-acid-CoA ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0220|M.bovis_AF2122/97 ----MPRGELYKRFRLVMGGIAPCGSGRRAATYPRRMQIRPY--------
Rv0214|M.tuberculosis_H37Rv ----MPRGELYKRFRLVMGGIAPCGSGRRAATYPRRMQIRPY--------
Mvan_0252|M.vanbaalenii_PYR-1 -----------------MTA-------APVPPIGTQVSALAA--------
Mflv_0419|M.gilvum_PYR-GCK -----------------MTEGQASAVDAPVPPISTQVSALAA--------
MSMEG_0318|M.smegmatis_MC2_155 -----------------MTDK-----GFDVPPIGTQVAHLAA--------
TH_3407|M.thermoresistible__bu -----------------MADTT-DVPGTPVPPIGTRFAALAQ--------
MAB_2316|M.abscessus_ATCC_1997 -----------------MAVMT---DAAVGEPFVRILANLAD--------
MMAR_3983|M.marinum_M ------------MSTQPMGPGATTNPLADGVPFGTRLEELAE--------
MUL_3844|M.ulcerans_Agy99 ------------MSTQPMGPGTTTNPLADGVPFGTRLEELAE--------
MAV_1627|M.avium_104 -------------MTESAPAELAANPLADGIPFGAKLQQLAG--------
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
. .
Mb0220|M.bovis_AF2122/97 --IAADKPAVILYPSG---TVISFDELEARANRLAHWFRQAGLREDDVVA
Rv0214|M.tuberculosis_H37Rv --IGADKPAVILYPSG---TVISFDELEARANRLAHWFRQAGLREDDVVA
Mvan_0252|M.vanbaalenii_PYR-1 --QAPDAPAATCDG-----VTVTRRELDATSNRLARAFASLGVGVGDYVT
Mflv_0419|M.gilvum_PYR-GCK --RSPDAAAITCAG-----RTVTRAELDATSNRIARGFASRGVGIGDYVT
MSMEG_0318|M.smegmatis_MC2_155 --ADPDRPAVSCQG-----RTLSRGELDRSTNRLARAYAELGVRQGDYVT
TH_3407|M.thermoresistible__bu --TDPTAPAVSCAG-----VTLTRRELDASTNRLARAFAELGVGQGDYVT
MAB_2316|M.abscessus_ATCC_1997 --AEPDAPAVTCGT-----DSVTRAELERRATALAHAYQRLGVRQGDLVT
MMAR_3983|M.marinum_M --QRRDETAVTIVAPDGSTQSMTFGELDCLANQWGRTLAAHGAQTGSLVA
MUL_3844|M.ulcerans_Agy99 --QRRDETAVTIVAPEGSTQSMTFGELDRLANQWGRTLAAHGAQTGSLVA
MAV_1627|M.avium_104 --QRGDDTAVTVVALDGTATSLTFAELDARANQWGRALAATGAQTGSLVA
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
: :: **: : .. * .. ::
Mb0220|M.bovis_AF2122/97 ILMENNEHVHAVMWAARRSGLYYVPINTHLTASEAAYIVDNSGAKAIVGS
Rv0214|M.tuberculosis_H37Rv ILMENNEHVHAVMWAARRSGLYYVPINTHLTASEAAYIVDNSGAKAIVGS
Mvan_0252|M.vanbaalenii_PYR-1 IVLPNSLDWAYSVLACWKLGAVPQPLSARLPDAELAALLELRRPALLVGR
Mflv_0419|M.gilvum_PYR-GCK IVLPNSIDWAYAVLACWKLGAVPQPLSARMPDAELGALLQLRRPALLVGR
MSMEG_0318|M.smegmatis_MC2_155 ICLHNSIEWVQATLATWKLGAVPQPLSPRMPDAEFEAILGLKRRALVVGR
TH_3407|M.thermoresistible__bu ISLFNSIEWLQATLAVWKLGAIPQPLSPRLPDPEFAELLALRRRALLVGR
MAB_2316|M.abscessus_ATCC_1997 IGLPNSVEFFAAQIAVWKLGATPQPVSWRLPLAERRAIVELADSTLVVGV
MMAR_3983|M.marinum_M LAIPNSQHLVLATLGCWKIGAVPIPMHWDLPEWERDRVRAVIDPAVIVDE
MUL_3844|M.ulcerans_Agy99 LAIPNSQHLVLATLGCWKIGAVPIPMHWDLPEWERDRVRAVIDPAVIVDE
MAV_1627|M.avium_104 LAIPNSQHLVLATLGCWKIGAVPVPMHWDLPEWERDRVREVINPAVVVDE
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
: * . : * : :. : : ::
Mb0220|M.bovis_AF2122/97 AALRETCHGLAEHLPGGLPDLLMLAGGGLVG--WMTYPECVADQPDTPIE
Rv0214|M.tuberculosis_H37Rv AALRETCHGLAEHLPGGLPDLLMLAGGGLVG--WMTYPECVADQPDTPIE
Mvan_0252|M.vanbaalenii_PYR-1 PDPTGVTPDVAVELAA--EFPD------------SPLPEAVSPVWKS---
Mflv_0419|M.gilvum_PYR-GCK TDPTGLTAGTTTDLAR--EFADESD---------AALPEAVSPVWKA---
MSMEG_0318|M.smegmatis_MC2_155 -DPGGDISWVPGDFEPGPGLAD------------DPLPEAVSPTWKS---
TH_3407|M.thermoresistible__bu PAPDGSIPHVPAGFTPDPALSD------------GPLPEAVSPVLKS---
MAB_2316|M.abscessus_ATCC_1997 -DPADHPDRVCVSAGFEPGAEDYLA---------GPVPEVVSPAWKA---
MMAR_3983|M.marinum_M HNRWRLQAHAAGESRD-------------------PLPAAVSPTANG---
MUL_3844|M.ulcerans_Agy99 HNRWRLQAHAAGESRD-------------------PLPTAVSPTANG---
MAV_1627|M.avium_104 TNRWGLEARAAGESEA-------------------ALPVAVSPTANG---
MLBr_02546|M.leprae_Br4923 DEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSSSAPAP
. . :: .
Mb0220|M.bovis_AF2122/97 DERE-GDLLQYSSGTTGRPKGIKRELPHVSPDAAPGMMPALLDFWMDADS
Rv0214|M.tuberculosis_H37Rv DERE-GDLLQYSSGTTGRPKGIKRELPHVSPDAAPGMMPALLDFWMDADS
Mvan_0252|M.vanbaalenii_PYR-1 ---------MASGGSTGRPKLIEAGNDSRVP-PAIGYPLG-----AQEGD
Mflv_0419|M.gilvum_PYR-GCK ---------MASGGSTGRPKLIEAGNDSRVP-PAIGYPLG-----AQEGD
MSMEG_0318|M.smegmatis_MC2_155 ---------IASGGSTGRPKLIEAGGDGRVP-ALIGAPLG-----AEPDD
TH_3407|M.thermoresistible__bu ---------MASGGSTGRPKLIESGGDARVP-PVIGTPLG-----AEPGD
MAB_2316|M.abscessus_ATCC_1997 ---------PTSGGSTGRPKIIVAPTPAVITGTAAGEIMG-----MQHAD
MMAR_3983|M.marinum_M ---------ICSSGSTGVPKVILNLAPSLWT-PQHGEPFLSAWTPVAQPQ
MUL_3844|M.ulcerans_Agy99 ---------ICSSGSTGVPKVILNLAPSLWT-PEHGEPFLSAWTPVAQPQ
MAV_1627|M.avium_104 ---------ICSSGSTGVPKVILNLAPSLWI-PQHGEPFLSNWTPVAQPQ
MLBr_02546|M.leprae_Br4923 KVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVP----FRAHE
:.*:** ** .
Mb0220|M.bovis_AF2122/97 VYLSPAPMYHTAPSVWTMSALAAGVTTVVMEKFDAEGALDAIQRYRVTHA
Rv0214|M.tuberculosis_H37Rv VYLSPAPMYHTAPSVWTMSALAAGVTTVVMEKFDAEGALDAIQRYRVTHA
Mvan_0252|M.vanbaalenii_PYR-1 VTLVSVPLSHNTGFTTFAVGLLQGHHLVVMPRFEPHEFLRLVTEHRVTFL
Mflv_0419|M.gilvum_PYR-GCK VNLVSVPLSHNTGFTTFAIGLLQGHHLVVMPRFEPREFLRLITEHRVTFL
MSMEG_0318|M.smegmatis_MC2_155 VTLMSVPMSHNTGFTTFAIALLMGQHLVLMPRFEPAEFLRLVTSHRVTFL
TH_3407|M.thermoresistible__bu VNLISVPLSHNTGFTTAVIGLVQGHHLVLMPRFDAAEFLRLVTDFRVTWL
MAB_2316|M.abscessus_ATCC_1997 AQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWL
MMAR_3983|M.marinum_M TIMVPAPMYHTNGFATFLM-LLGGDHLVVLERFDAALVVDVIERFRVTNF
MUL_3844|M.ulcerans_Agy99 TIMVPAPMYHTNGFATFMM-LLGGDHLVVLEKFDAALVVDVIERFRVTNF
MAV_1627|M.avium_104 TIMVPAPMYHTNGFAPLLM-LLGGDHLVILEKFDAALVLDVIERFRITNF
MLBr_02546|M.leprae_Br4923 VTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATVLEDIEKHQATAM
. : . *: * : * *: :*.. : : .: *
Mb0220|M.bovis_AF2122/97 QFVPAMFVRMLKLPEAVR----NSYDMSSLRRVIHAAAPCPVQIKEQMIH
Rv0214|M.tuberculosis_H37Rv QFVPAMFVRMLKLPEAVR----NSYDMSSLRRVIHAAAPCPVQIKEQMIH
Mvan_0252|M.vanbaalenii_PYR-1 TTVPTIMQRLLPVYR----ADPDAYDLSSIRRFWHLAAPCPPAVKHAWIE
Mflv_0419|M.gilvum_PYR-GCK TTVPTIMQRLLPVYR----ADPDAYDLSSIRRFWHVASACPPAVKRAWID
MSMEG_0318|M.smegmatis_MC2_155 TTVPTIMQRLLPVYR----AEPDAYDLTSIRRFWHVGAPCPPAVKQAWID
TH_3407|M.thermoresistible__bu TTVPTIMQRLLPVYRAAAAADPGRYDLSSLRRFWHVGAPCPPAVKEAWIE
MAB_2316|M.abscessus_ATCC_1997 QVVPTMMSRMLRELR----QHPGRFDLSTIRVLWHMAAPCPVWLKQAWID
MMAR_3983|M.marinum_M TATPTMLARIAAIPG------IRQRDLSSIVFILQGAAVMPPSLLHTWFE
MUL_3844|M.ulcerans_Agy99 TATPTMLARIAAIPG------IRQRDLSSIVFILQGAAVMPPSLLHTWFE
MAV_1627|M.avium_104 TATPTMLARIAARPD------VRQRDLSSIVFILQGAAVMPPSLLHTWFE
MLBr_02546|M.leprae_Br4923 VVVPVMLSRILDTLEN----LETKSDLSSLRVVFVSGSQLGAELATRALE
.*.:: *: *:::: . .: : :.
Mb0220|M.bovis_AF2122/97 WWGP-IIDEYYASSEASGSTLITAEDWLTHPGSVGKPIQG-GVHIVGADG
Rv0214|M.tuberculosis_H37Rv WWGP-IIDEYYASSEASGSTLITAEDWLTHPGSVGKPIQG-GVHIVGADG
Mvan_0252|M.vanbaalenii_PYR-1 LLGPDAVWELYGGTELQALTFISGDQWLTHPGSVGVVVAG-EMKVLDDDG
Mflv_0419|M.gilvum_PYR-GCK IVGPDALWELYGGTELQALTFISGTQWLTHPGSVGVVVAG-EMKVLDDDG
MSMEG_0318|M.smegmatis_MC2_155 LLGPQVVWELYGGTELQALTFISGEQWLTHPGSVGVVVAG-EMKVLDDDG
TH_3407|M.thermoresistible__bu ILGPQVLWELYGGTELQALTFISGEQWLSHRGSVGVVVSG-EMKVLDDDG
MAB_2316|M.abscessus_ATCC_1997 LLGPEVIWELYAGTEAIGGTIITGQEWLAHRGSVGKPALG-ELAILDENG
MMAR_3983|M.marinum_M LLGPEKIVTAYGMTENLGLTALRGDEWVSHPGSVGRGFRETEIQILDSQQ
MUL_3844|M.ulcerans_Agy99 LLGPEKIVTAYGMTENLGLTALRGDEWVSHPGSVGRGFRETEIQILDSQQ
MAV_1627|M.avium_104 LLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDSQQ
MLBr_02546|M.leprae_Br4923 ELGP-VIYNMYGSTEIAFATIAGPKDLQINSATVGPVVKGVKVKILDDSG
.* : *. :* * : : .:** : ::. .
Mb0220|M.bovis_AF2122/97 SELPPNQPGEIYFEGG--YPFEYLNDPAKTAASRNKHGWVTVGDVGYLDD
Rv0214|M.tuberculosis_H37Rv SELPPNQPGEIYFEGG--YPFEYLNDPAKTAASRNKHGWVTVGDVGYLDD
Mvan_0252|M.vanbaalenii_PYR-1 DECPPGVAGEIYMRSAPGGPPTYRYVG---STAKSRDGWESLGDLGYFDE
Mflv_0419|M.gilvum_PYR-GCK NECPPGVPGEIYMRPAPGARPTYRYIG---STAKSRDGWDSLGDLGYFDE
MSMEG_0318|M.smegmatis_MC2_155 NECPPGVTGEVYMRPSPGSTPTYRYVG---TTAKSRDGWDSLGDLGYFDT
TH_3407|M.thermoresistible__bu NECPPVVVGEIYMRPSPGSGPTYRYVG---ATAKSRDGWDSLGDLGYFDE
MAB_2316|M.abscessus_ATCC_1997 NPLPAGEVGEIFMRPWEGMPTPYKYLG---AEIKRFGTWESIGDLGWLDD
MMAR_3983|M.marinum_M QPVGPGEHGDVYLRAP--MSAGYRYLGGAPPLPSTGEGYRCAGDIGYLDE
MUL_3844|M.ulcerans_Agy99 QPVGPGEHGDVYLRAP--MSAGYRYLGGAPPLPSTGEGYRCAGDIGYLDE
MAV_1627|M.avium_104 RPLGPGEDGDVYLRAP--MSAGYRYLGGAPPLPSTEDGFRSAGDIGHLDE
MLBr_02546|M.leprae_Br4923 KEVPRGQVGRIFVGNAFPFEGYTGGGG-----KQIIDGLLSSGDVGYFDE
* ::. **:* :*
Mb0220|M.bovis_AF2122/97 DGYLFLTGRRHHMIISGGVNIYPQEAENLLVAHPKVLDAAVFGVPDDEMG
Rv0214|M.tuberculosis_H37Rv DGYLFLTGRRHHMIISGGVNIYPQEAENLLVAHPKVLDAAVFGVPDDEMG
Mvan_0252|M.vanbaalenii_PYR-1 DGFLYLNDRRVDMFTVGGRNVYPAEIESALAEHPRVLSSLAMGLPDDDLG
Mflv_0419|M.gilvum_PYR-GCK DGFLYLNDRRVDMFTVGGRNVYPAEIESALAEHPGVLSSLVVGLPDDDLG
MSMEG_0318|M.smegmatis_MC2_155 DGFLYLNDRRVDMFTVGGRNVYPAEIEAALSAHPEVLSCLAVGIPHTDLG
TH_3407|M.thermoresistible__bu DGFLYLSDRRVDMFTVGGRNVYPAEVESALAEHPEVLSALVVGIPDDDLG
MAB_2316|M.abscessus_ATCC_1997 DGYLYMSDRRTDLIVTGGANVFPAEVESVMNGYPGVIDSVVVGLPDDDLG
MMAR_3983|M.marinum_M DGYLYIVDRRVDMIVTGGANVFPAEVESALAGHPGIADVVVIGIADARWG
MUL_3844|M.ulcerans_Agy99 DGYLYIVDRRVDMIITGGANVFPAEVESALAGHPGIADVVVIGIADARWG
MAV_1627|M.avium_104 DGYLYIVDRRVDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWG
MLBr_02546|M.leprae_Br4923 HDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIGVDDKEWG
.. *:: .* .:: ** *::* * * : :* : . ..*: . *
Mb0220|M.bovis_AF2122/97 QRVMAAVQTVDSADANDQFAGELLAWLRDRLSHFKCPRSIAFEPQLPRTD
Rv0214|M.tuberculosis_H37Rv QRVMAAVQTVDSADANDQFAGELLAWLRDRLSHFKCPRSIAFEPQLPRTD
Mvan_0252|M.vanbaalenii_PYR-1 QVPHAIVQAHG------LDEAEVIAFLAERIASHKLPRSVEFTDRPLRDD
Mflv_0419|M.gilvum_PYR-GCK HVPHAIVQAD-------LSEADVLAFLGERIASYKLPRTVEFTDRPLRDD
MSMEG_0318|M.smegmatis_MC2_155 QVPHALVQTAP---GSTLDAAGLRDFLRDAVSSYKVPHTIEFVDEPLRDD
TH_3407|M.thermoresistible__bu QVPYAIVHTAD---GSDLDEAAVIGFLRERIAGYKVPRHVEFSATPVRDD
MAB_2316|M.abscessus_ATCC_1997 QSVHAVVHAEP---N--VTEDQLRDYLLDQIVRYKVPRTIEFVADPLRED
MMAR_3983|M.marinum_M RRVHAVVQVA-----APLTEQQVIEYAKNRLAPYKVPKTVEFVDRIPRTA
MUL_3844|M.ulcerans_Agy99 RRVHAVVQVA-----APLTEQQVIEYAKNRLAPYKVPKTVEFVDQIPRTA
MAV_1627|M.avium_104 RRVHAVVQRAD---GASLTEQQVIDYAKGRLAPYKAPKTVEFVDAIPRTA
MLBr_02546|M.leprae_Br4923 ARLRAFVVKKQ---DATIDEDAIKAYVRDHLARYKVPREVIFLEELPRNP
* * : : : .* *: : * *
Mb0220|M.bovis_AF2122/97 TGKLYKSGLVEKYSV---------
Rv0214|M.tuberculosis_H37Rv TGKLYKSGLVEKYSV---------
Mvan_0252|M.vanbaalenii_PYR-1 AGKARRSAVRDEVIARRSS-----
Mflv_0419|M.gilvum_PYR-GCK AGKARRSAVRDEVLARRA------
MSMEG_0318|M.smegmatis_MC2_155 AGKARRTAVRDAVIARLGRAPAAR
TH_3407|M.thermoresistible__bu AGKARRSAVRDEVIARSASRRS--
MAB_2316|M.abscessus_ATCC_1997 SGKVRRSLIREQATARRAAAG---
MMAR_3983|M.marinum_M ATKVNRSAM---IAARGG------
MUL_3844|M.ulcerans_Agy99 ATKVNRSAM---IAARGG------
MAV_1627|M.avium_104 ATKVNRSAM---IAARGG------
MLBr_02546|M.leprae_Br4923 TGKVLKRELRDFEVH---------
: * : :