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TRSSSQGSSRSNSEYSDVADMFRELAELEEGSAAFQRQRDRIVERCLPLADHIARRFDGRGEPRDDLVQV ARVGLVNAVIRFDVTAGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRA PTPSELAAELEMDRDEVVEGLVAGSSYNTLSIDSGGGSGNEDAPAIADTLGDVDLTLDQIENREALRPLL AALPERERTVLVLRFFESMTQTQIAERVGISQMHVSRLLAKSLTRLRDQLE*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4789 | - | - | 100% (262) | RNA polymerase sigma factor SigF |
| M. gilvum PYR-GCK | Mflv_3871 | - | 3e-84 | 62.20% (254) | FliA/WhiG family RNA polymerase sigma factor |
| M. gilvum PYR-GCK | Mflv_3872 | - | 4e-79 | 60.16% (251) | FliA/WhiG family RNA polymerase sigma factor |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3314c | sigF | 1e-116 | 81.68% (262) | RNA polymerase sigma factor SigF |
| M. tuberculosis H37Rv | Rv3286c | sigF | 1e-116 | 81.68% (262) | RNA polymerase sigma factor SigF |
| M. leprae Br4923 | MLBr_01022 | rpoT | 4e-22 | 29.13% (230) | RNA polymerase sigma factor |
| M. abscessus ATCC 19977 | MAB_2511 | - | 3e-79 | 57.37% (251) | RNA polymerase sigma factor SigF |
| M. marinum M | MMAR_1248 | sigF | 1e-119 | 83.97% (262) | alternate RNA polymerase sigma factor SigF |
| M. avium 104 | MAV_4256 | - | 1e-115 | 87.97% (241) | RNA polymerase sigma factor SigF |
| M. smegmatis MC2 155 | MSMEG_1804 | - | 1e-119 | 87.55% (249) | RNA polymerase sigma factor SigF |
| M. thermoresistible (build 8) | TH_0241 | sigF | 1e-120 | 92.15% (242) | ALTERNATE RNA POLYMERASE SIGMA FACTOR SIGF |
| M. ulcerans Agy99 | MUL_2640 | sigF | 1e-119 | 83.97% (262) | RNA polymerase sigma factor SigF |
| M. vanbaalenii PYR-1 | Mvan_1660 | - | 1e-135 | 95.44% (263) | RNA polymerase sigma factor SigF |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3314c|M.bovis_AF2122/97 --------------------------------------------------
Rv3286c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1248|M.marinum_M --------------------------------------------------
MUL_2640|M.ulcerans_Agy99 --------------------------------------------------
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1660|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_01022|M.leprae_Br4923 MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSP
Mb3314c|M.bovis_AF2122/97 --------------------------------------------------
Rv3286c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1248|M.marinum_M --------------------------------------------------
MUL_2640|M.ulcerans_Agy99 --------------------------------------------------
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1660|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_01022|M.leprae_Br4923 TEGVYVVATKTSPATGQPVKRTATKTPATPAKRSTTKAANGSAPTKRATS
Mb3314c|M.bovis_AF2122/97 --------------------------------------------------
Rv3286c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1248|M.marinum_M --------------------------------------------------
MUL_2640|M.ulcerans_Agy99 --------------------------------------------------
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1660|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_01022|M.leprae_Br4923 TASRATKSTTPPKASGASDAAKKARTSVKSVDTKAPSAQDHTTKASAAKD
Mb3314c|M.bovis_AF2122/97 --------------------------------------------------
Rv3286c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1248|M.marinum_M --------------------------------------------------
MUL_2640|M.ulcerans_Agy99 --------------------------------------------------
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1660|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_01022|M.leprae_Br4923 TLNDPEATLNTLDSDATADDLDAEPDLDVEPSEDIDIDIEAADLGLDDLE
Mb3314c|M.bovis_AF2122/97 --------------------------------------------------
Rv3286c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1248|M.marinum_M --------------------------------------------------
MUL_2640|M.ulcerans_Agy99 --------------------------------------------------
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1660|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_01022|M.leprae_Br4923 DDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTEKDKASGDFVW
Mb3314c|M.bovis_AF2122/97 -----------------------------------MTARAAGGSASRANE
Rv3286c|M.tuberculosis_H37Rv -----------------------------------MTARAAGGSASRANE
MMAR_1248|M.marinum_M -----------------------------------MTSRTAGGSSSRPNE
MUL_2640|M.ulcerans_Agy99 -----------------------------------MTSRTAGGSSSRPNE
MAV_4256|M.avium_104 --------------------------------------------------
Mflv_4789|M.gilvum_PYR-GCK -----------------------------------MTRSSSQGSS-RSNS
Mvan_1660|M.vanbaalenii_PYR-1 -----------------------------------MTRSSSQGSSSRSGS
TH_0241|M.thermoresistible__bu --------------------------------------------------
MSMEG_1804|M.smegmatis_MC2_155 -----------------------------------MTS------------
MAB_2511|M.abscessus_ATCC_1997 --------------------------MECPVDVPETGHSQVKPRVGRPTD
MLBr_01022|M.leprae_Br4923 DEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIE
Mb3314c|M.bovis_AF2122/97 -YADVPEMFRELVGLPAGSPEFQRH------------RDKIVQRCLPLAD
Rv3286c|M.tuberculosis_H37Rv -YADVPEMFRELVGLPAGSPEFQRH------------RDKIVQRCLPLAD
MMAR_1248|M.marinum_M -YADVPEMFRELATFDADSPNFQRH------------RDTIVERCLPLAD
MUL_2640|M.ulcerans_Agy99 -YADVPEMFRELATFDADSPNFQRH------------RDTIVERCLPLAD
MAV_4256|M.avium_104 -------MFRELATAEPDSMEFQRQ------------RDKIVERCLPLAD
Mflv_4789|M.gilvum_PYR-GCK EYSDVADMFRELAELEEGSAAFQRQ------------RDRIVERCLPLAD
Mvan_1660|M.vanbaalenii_PYR-1 EYADVAPMFRELAELEEGTAAFQRQ------------RDRIVERCLPLAD
TH_0241|M.thermoresistible__bu -------MFRELRKLPAESKEFQRQ------------RDRIVERCLPLAD
MSMEG_1804|M.smegmatis_MC2_155 EYADVLDMFRELAGTTEGSPSYVRQ------------REKIVERCLPLAD
MAB_2511|M.abscessus_ATCC_1997 DYADVPDMFRSLAGLAEHSDAYQRK------------HEEIVTRCLPLAD
MLBr_01022|M.leprae_Br4923 AGLYATQLMTELSERGTKLPTAQRRDMMWICRDGDRAKNHLLEANLRLVV
:: .* *: :: :: * *.
Mb3314c|M.bovis_AF2122/97 HIARRFEGRGEPRDDLIQVARVGLVNAAVRFDVKTGSDFVSFAVPTIMGE
Rv3286c|M.tuberculosis_H37Rv HIARRFEGRGEPRDDLIQVARVGLVNAAVRFDVKTGSDFVSFAVPTIMGE
MMAR_1248|M.marinum_M HIARRFEGRGEPRDDLVQVARVGLVNAVVRFDVETGSDFVSFAVPTIMGE
MUL_2640|M.ulcerans_Agy99 HIARRFEGRGEPRDDLVQVARVGLVNAVVRFDVETGSDFVSFAVPTIMGE
MAV_4256|M.avium_104 HIARRFEGRGEPRDDLVQVARVGLVNAVVRFDVDAGSDFVSFAVPTIMGE
Mflv_4789|M.gilvum_PYR-GCK HIARRFDGRGEPRDDLVQVARVGLVNAVIRFDVTAGSDFVSFAVPTIMGE
Mvan_1660|M.vanbaalenii_PYR-1 HIARRFDGRGEPRDDLVQVARVGLVNAVIRFDVNAGSDFVSFAVPTIMGE
TH_0241|M.thermoresistible__bu HIARRFDGRGEARDDLVQVARVGLVNAVIRFDVETGSDFVSFAVPTIMGE
MSMEG_1804|M.smegmatis_MC2_155 HIARRFDGRGEPREDLVQVARVGLVNAVNRFDVEAGSDFVSFAVPTIMGE
MAB_2511|M.abscessus_ATCC_1997 NVARHFDRRGEDLEDLIQVARVGLLNAVNRFDPEKGASFLGFAVPTMMGE
MLBr_01022|M.leprae_Br4923 SLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
:*::: ** **:* ..:**:.*. :** * .* :*. :
Mb3314c|M.bovis_AF2122/97 VRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELG
Rv3286c|M.tuberculosis_H37Rv VRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELG
MMAR_1248|M.marinum_M VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTATELAAELE
MUL_2640|M.ulcerans_Agy99 VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTATELAAELE
MAV_4256|M.avium_104 VRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAHTATELAAELE
Mflv_4789|M.gilvum_PYR-GCK VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTPSELAAELE
Mvan_1660|M.vanbaalenii_PYR-1 VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTATELAAELE
TH_0241|M.thermoresistible__bu VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTASELAEELE
MSMEG_1804|M.smegmatis_MC2_155 VRRHFRDNSWSVKVPRRLKELHLRLGAATAELSQRLGRAPTASELAHELD
MAB_2511|M.abscessus_ATCC_1997 VRRHFRDHGWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAELG
MLBr_01022|M.leprae_Br4923 ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMD
: * : *:. ::::* : : ::. :* * * * :. *** *:
Mb3314c|M.bovis_AF2122/97 MDRAEVIEGLLAGSSYHTLSIDSGGG-S-DDDARAITDTLGDVDAGLDQI
Rv3286c|M.tuberculosis_H37Rv MDRAEVIEGLLAGSSYHTLSIDSGGG-S-DDDARAITDTLGDVDAGLDQI
MMAR_1248|M.marinum_M MDRAEVVEGLVAGSSYNTLSIDSGSG-SEDDDARAIADTLGDMDAGLDRI
MUL_2640|M.ulcerans_Agy99 MDRAEVVEGLVAGSSYNTLSIDSGSG-SEDDDARAIADTLGDMDAGLDRI
MAV_4256|M.avium_104 MDREEVVEGLVAGSSYNTLSIDSGGG-SEEEEVRAIADTLGDVDTGLDRI
Mflv_4789|M.gilvum_PYR-GCK MDRDEVVEGLVAGSSYNTLSIDSGGG-SGNEDAPAIADTLGDVDLTLDQI
Mvan_1660|M.vanbaalenii_PYR-1 MDRDEVVEGLVAGSSYNTLSIDSGG--SGNEDAPAIADTLGDVDLSLDQI
TH_0241|M.thermoresistible__bu MDRDEVIEGLVAGSSYNTLSIDSGGSGSGTEDAPAIADTLGDVDTGLDQI
MSMEG_1804|M.smegmatis_MC2_155 MDREEVVEGLIAGSSYNTLSIDSGGG--GDEEAPAIVDTLGDLDMGLDQI
MAB_2511|M.abscessus_ATCC_1997 IDREEVVESLIAADAYQPQSIEAPLS-HGDQESKHLSDLLGNEDPALEHV
MLBr_01022|M.leprae_Br4923 ITPEKVLE--IQQYAREPISLDQTIG---DEGDSQLGDFIEDSEAVVAVD
: :*:* : : .. *:: : : * : : : :
Mb3314c|M.bovis_AF2122/97 EN-----REVLRPLLEALPERERTVLVLRFFDS----MTQTQIAERVGIS
Rv3286c|M.tuberculosis_H37Rv EN-----REVLRPLLEALPERERTVLVLRFFDS----MTQTQIAERVGIS
MMAR_1248|M.marinum_M ED-----RESLRPLLEALPERERTVLVLRFFDS----MTQTQIAERVGIS
MUL_2640|M.ulcerans_Agy99 ED-----RESLRPLLEALPERERTVLVLRFFDS----MTQTQIAERVGIS
MAV_4256|M.avium_104 ED-----QESLRPLLEALPERERTVLVLRFFES----MTQTQIAERVGIS
Mflv_4789|M.gilvum_PYR-GCK EN-----REALRPLLAALPERERTVLVLRFFES----MTQTQIAERVGIS
Mvan_1660|M.vanbaalenii_PYR-1 EN-----REALRPLLAALPERERTVLALRFFES----MTQTQIAERVGIS
TH_0241|M.thermoresistible__bu EN-----REALRPLLAALPERERTVLVLRFFES----LTQTQIAERVGIS
MSMEG_1804|M.smegmatis_MC2_155 DN-----RESLRPLLASLPERERTVLVLRFFES----MTQTQIAERVGIS
MAB_2511|M.abscessus_ATCC_1997 TN-----REAVRPLLANLPLRERTVLELRFFKG----MTQSQIAAQIGVS
MLBr_01022|M.leprae_Br4923 AVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVT
:: ::.:* *. ** *: *** * :*. *::
Mb3314c|M.bovis_AF2122/97 QMHVSRLLAKSLARLRDQLE---------
Rv3286c|M.tuberculosis_H37Rv QMHVSRLLAKSLARLRDQLE---------
MMAR_1248|M.marinum_M QMHVSRLLAKSLARLRDQLE---------
MUL_2640|M.ulcerans_Agy99 QMHVSRLLAKSLARLRDQLE---------
MAV_4256|M.avium_104 QMHVSRLLAKSLTRLRDQLQ---------
Mflv_4789|M.gilvum_PYR-GCK QMHVSRLLAKSLTRLRDQLE---------
Mvan_1660|M.vanbaalenii_PYR-1 QMHVSRLLAKSLARLRDQLE---------
TH_0241|M.thermoresistible__bu QMHVSRLLAKSLARLRDQLE---------
MSMEG_1804|M.smegmatis_MC2_155 QMHVSRLLAKSLARLRDQLQ---------
MAB_2511|M.abscessus_ATCC_1997 QMHVSRILSDTLRYLREQMA---------
MLBr_01022|M.leprae_Br4923 RERIRQIESKTMSKLRHPSRSQVLRDYLD
: :: :: :.:: **.