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ESDPEDQGNEPPEINFDEHLHPARPRSLRFRPRVRTPFTRRSLSRDGSPTGDNPAYVSWLISQSMLADAN EISQQFSGQGSMWQNPYATPSARGAVETASVWFTAYPLSLITRSNESFLKAMADADMWKAFAEIGIEAVH TGPVKRAGGISGWELTPSVDGHFDRISTQIDPAFGTEDEFRQMCGTANWYGGTIIDDIVPGHTGKGADFR LAEMKYADYPGIYHMVEVDPRDWEHLPEVPAGADSVNIDSDTEDWLDKAGYIIGKLQRVIFYAEGIKETN WSVTRPVVGIDGVERRWVYLHYFKDGQPSINWLDPSFAGMRLVIGDALHSLTDLGTGGLRLDANGFLGAE KTAAEDSAAWSEGHPLSEAANHLIASMVRKVGGFTFQELNLTIDDIREIGEAGADLSYDFITRPGVQHAL ATADTEFLRLTLRTTLELGVDPASLVHALQNHDELTYELVHWSNGHRDDLFPYKGEEVTGEVLGDIVRTD LREKLSGDNAPYNLVFTTNGIACTSATIIAATLGITSLDDITEPEQIDRIRRAHLLLAMFNALQPGVFAL SGWDVCGMLTLPSSTIPELLREGDTRWIHRAAHDLMGVNPEATQSPAGMPRGRSLYGSIPEQLADDTSFL RQLQAILKVRSHYGIATSRQIDIPEVSQRGMLVLVHHLDTDNQYQLTVLNFANEDVTGTVRSKKLPPGSE VSDMFSGRHLATVDDLHSFSVELTAHQGLSLLVEAPAEDTDAD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1577 | - | - | 100% (744) | trehalose synthase |
| M. gilvum PYR-GCK | Mflv_3723 | - | 3e-09 | 22.54% (346) | alpha amylase, catalytic region |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0131 | treS | 9e-07 | 26.33% (319) | trehalose synthase TRES |
| M. tuberculosis H37Rv | Rv0126 | treS | 9e-07 | 26.33% (319) | trehalose synthase TRES |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_0325 | treS | 6e-07 | 27.67% (318) | trehalose synthase TreS |
| M. avium 104 | MAV_5186 | treS | 1e-06 | 25.79% (318) | trehalose synthase |
| M. smegmatis MC2 155 | MSMEG_6515 | treS | 7e-08 | 27.13% (317) | trehalose synthase |
| M. thermoresistible (build 8) | TH_1780 | treS | 2e-07 | 26.73% (318) | trehalose synthase |
| M. ulcerans Agy99 | MUL_4797 | treS | 3e-07 | 27.67% (318) | trehalose synthase TreS |
| M. vanbaalenii PYR-1 | Mvan_5178 | - | 0.0 | 91.03% (747) | trehalose synthase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_6515|M.smegmatis_MC2_155 -------------MEEH-TQGSHVEAG----IVEHPNAEDFGHARTLPTD
TH_1780|M.thermoresistible__bu MESTSEPDRR---TEHHRSEGSHVEHGPAGSVVEHPTADDFGQARVLPED
Mb0131|M.bovis_AF2122/97 ---MNEAEHS---VEHPPVQGSHVEGG----VVEHPDAKDFGSAAALPAD
Rv0126|M.tuberculosis_H37Rv ---MNEAEHS---VEHPPVQGSHVEGG----VVEHPDAKDFGSAAALPAD
MMAR_0325|M.marinum_M --MMNEATNT---IEHNPAEGSHVEGG----VVEHPDAKDFGNAAALPAD
MUL_4797|M.ulcerans_Agy99 MELMNEATNT---IEHNPAEGSHVEGG----MVEHPDAKDFGNAAPLPAD
MAV_5186|M.avium_104 ---MNDAREA---VEHHPEEGSHVQDG----VVEHPEAEDFDNAAALPTD
Mflv_1577|M.gilvum_PYR-GCK MESDPEDQGN---EPPEINFDEHLHPARPRSLRFRPRVRTPFTRRSLSRD
Mvan_5178|M.vanbaalenii_PYR-1 MESEPEDQGTDSSESPELTFDEHLHPARPRSLRFRPRVRAPFTRRSLARD
..*:. . : :* . *. *
MSMEG_6515|M.smegmatis_MC2_155 --TNWFKHAVFYEVLVRAFYDSNADGIGDLRG------------------
TH_1780|M.thermoresistible__bu --PTWYKHAVFYEVLVRAFYDSNADGIGDLRG------------------
Mb0131|M.bovis_AF2122/97 --PTWFKHAVFYEVLVRAFFDASADGSGDLRG------------------
Rv0126|M.tuberculosis_H37Rv --PTWFKHAVFYEVLVRAFFDASADGSGDLRG------------------
MMAR_0325|M.marinum_M --PTWFKHAVFYEVLVRAFYDGTADGSGDLRG------------------
MUL_4797|M.ulcerans_Agy99 --PTWFKHAVFYEVLVRAFYDGTADGSGDLRG------------------
MAV_5186|M.avium_104 --PTWFKHAVFYEVLVRAFFDANADGAGDLRG------------------
Mflv_1577|M.gilvum_PYR-GCK GSPTGDNPAYVSWLISQSMLADANEISQQFSGQGSMWQNPYATPSARGAV
Mvan_5178|M.vanbaalenii_PYR-1 GSPTGDNPAYVSWLISQSMLADANEISQQFSGQGSMWQNPYATPSPRGAV
.. : * . :: ::: : :: *
MSMEG_6515|M.smegmatis_MC2_155 ----------------------LTEKLDYIKWLGVDCLWLPPFYDSPLRD
TH_1780|M.thermoresistible__bu ----------------------LTEQLDYLKWLGVDCLWLPPFYDSPLRD
Mb0131|M.bovis_AF2122/97 ----------------------LIDRLDYLQWLGIDCIWLPPFYDSPLRD
Rv0126|M.tuberculosis_H37Rv ----------------------LIDRLDYLQWLGIDCIWLPPFYDSPLRD
MMAR_0325|M.marinum_M ----------------------LMQRLDYLQWLGIDCIWLPPFYDSPLRD
MUL_4797|M.ulcerans_Agy99 ----------------------LMQRLDYLQWLGIDCIWLPPFYDSPLRD
MAV_5186|M.avium_104 ----------------------LLAQLDYLQWLGIDCIWLPPFYDSPLRD
Mflv_1577|M.gilvum_PYR-GCK ETASVWFTAYPLSLITRSNESFLKAMADADMWKAFAEIGIEAVHTGPVKR
Mvan_5178|M.vanbaalenii_PYR-1 ETASVWFTAYPLSLITRSNESFLKAMADVEMWKAFAEIGIEAVHTGPVKR
* * * .. : : ..: .*::
MSMEG_6515|M.smegmatis_MC2_155 GG-------------YDIRDFYKVLPEFGTVDDFVTLLDAAHRRGIRIIT
TH_1780|M.thermoresistible__bu GG-------------YDIRDFYKVLPEFGTVDDFVNLLDAAHRRGIRIIT
Mb0131|M.bovis_AF2122/97 GG-------------YDIRDFYKVLPEFGTVDDFVALVDAAHRRGIRIIT
Rv0126|M.tuberculosis_H37Rv GG-------------YDIRDFYKVLPEFGTVDDFVALVDAAHRRGIRIIT
MMAR_0325|M.marinum_M GG-------------YDIRDFYKVLPEFGTVGDFVALVDAAHRRGIRVIT
MUL_4797|M.ulcerans_Agy99 GG-------------YDIRDFYKVLPEFGTVGDFVALVDAAHRRGIRVIT
MAV_5186|M.avium_104 GG-------------YDIRDFYKVLPEFGTVEDFVALLNAAHERGIRVIT
Mflv_1577|M.gilvum_PYR-GCK AGGISGWELTPSVDGHFDRISTQIDPAFGTEDEFRQMCGTANWYGGTIID
Mvan_5178|M.vanbaalenii_PYR-1 AGGISGWELTPSVDGHFDRISTEIDPAFGTEDEFRQMCGTANWYGGTIID
.* : * :: * *** :* : .:*: * :*
MSMEG_6515|M.smegmatis_MC2_155 DLVMNHTSDQHEWFQESRHNPDGP------YGDFYVWSDTSDRYPDARII
TH_1780|M.thermoresistible__bu DLVMNHTSDTHPWFEESRRDPDGP------YGDFYVWSDTAEKYSQARII
Mb0131|M.bovis_AF2122/97 DLVMNHTSESHPWFQESRRDPDGP------YGDYYVWSDTSERYTDARII
Rv0126|M.tuberculosis_H37Rv DLVMNHTSESHPWFQESRRDPDGP------YGDYYVWSDTSERYTDARII
MMAR_0325|M.marinum_M DLVMNHTSESHPWFQESRHDPDGP------YGDYYVWSDSSEKYTDARII
MUL_4797|M.ulcerans_Agy99 DLVMNHTSESHPWFQESRHDPDGP------YGDYYVWSDSSEKYTDARII
MAV_5186|M.avium_104 DLVMNHTSESHPWFQESRHDPDGP------YGDFYVWSDTSDRYADARII
Mflv_1577|M.gilvum_PYR-GCK DIVPGHTGKGADFRLAEMKYADYPGIYHMVEVDPRDWEHLPEVPAGADSV
Mvan_5178|M.vanbaalenii_PYR-1 DIVPGHTGKGADFRLAEMKYADYPGIYHMVEVDPRDWEHLPDVPPGADSV
*:* .**.. : . : .* * * *.. .: . * :
MSMEG_6515|M.smegmatis_MC2_155 FVDTEESNWTFDP----------------------------------VRR
TH_1780|M.thermoresistible__bu FVDTEESNWTFDP----------------------------------VRR
Mb0131|M.bovis_AF2122/97 FVDTEESNWSFDP----------------------------------VRR
Rv0126|M.tuberculosis_H37Rv FVDTEESNWSFDP----------------------------------VRR
MMAR_0325|M.marinum_M FVDTEESNWTFDP----------------------------------VRR
MUL_4797|M.ulcerans_Agy99 FVDTEESNWTFDP----------------------------------VRR
MAV_5186|M.avium_104 FVDTEESNWTFDP----------------------------------VRR
Mflv_1577|M.gilvum_PYR-GCK NIDSDTEDWLDKAGYIIGKLQRVIFYAEGIKETNWSVTRPVVGIDGVERR
Mvan_5178|M.vanbaalenii_PYR-1 NIDPATEQWLDKAGYIIGKLQRVIFYAEGIKETNWSVTRPVVGIDGVERR
:*. .:* .. **
MSMEG_6515|M.smegmatis_MC2_155 QFYWHRFFSHQPDLNYDNP---AVQEAMLDVLRFWLDLGIDGFRLDAVPY
TH_1780|M.thermoresistible__bu QFYWHRFFSHQPDLNYDNP---AVQEAMLDVLRFWLDLGIDGFRLDAVPY
Mb0131|M.bovis_AF2122/97 QFYWHRFFSHQPDLNYDNP---AVQEAMIDVIRFWLGLGIDGFRLDAVPY
Rv0126|M.tuberculosis_H37Rv QFYWHRFFSHQPDLNYDNP---AVQEAMIDVIRFWLGLGIDGFRLDAVPY
MMAR_0325|M.marinum_M QFYWHRFFSHQPDLNYDNP---AVQEAMIDVLRFWLGLGIDGFRLDAVPY
MUL_4797|M.ulcerans_Agy99 QFYWHRFFSHQPDLNYDNP---AVQEAMIDVLRFWLGLGIDGFRLDAVPY
MAV_5186|M.avium_104 QFYWHRFFSHQPDLNYDNP---AVQEAMIDVIRFWLGLGIDGFRLDAVPY
Mflv_1577|M.gilvum_PYR-GCK WVYLHYFKDGQPSINWLDPSFAGMRLVIGDALHSLTDLGTGGLRLDANGF
Mvan_5178|M.vanbaalenii_PYR-1 WVYLHYFKDGQPSINWLDPSFAGMRLVIGDALHSLTDLGTGGLRLDANGF
.* * * . **.:*: :* .:: .: *.:: .** .*:**** :
MSMEG_6515|M.smegmatis_MC2_155 LFEREGTNCENLPETHAFLKRCRKAIDDEYPGRVLLAEANQWPADVVAYF
TH_1780|M.thermoresistible__bu LFEREGTNCENLPETHAFLKRCRKVVDDEYPGRVLLAEANQWPADVVAYF
Mb0131|M.bovis_AF2122/97 LFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPGDVVEYF
Rv0126|M.tuberculosis_H37Rv LFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPGDVVEYF
MMAR_0325|M.marinum_M LFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPADVVEYF
MUL_4797|M.ulcerans_Agy99 LFEREGTNCENLPETHAFLKRVRKVVDDEFPGRLLLAEANQWPADVVEYF
MAV_5186|M.avium_104 LFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPADVVEYF
Mflv_1577|M.gilvum_PYR-GCK LGAEKTAAEDSAAWSEG--HPLSEAANHLIASMVRKVGGFTFQELNLTID
Mvan_5178|M.vanbaalenii_PYR-1 LGAEKTAAEDSAAWSEG--HPLSEAANHLIASMVRKVGGFTFQELNLTID
* .: : :. . :.. : :. :. .. : . . : :
MSMEG_6515|M.smegmatis_MC2_155 GDPDTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEILAQTPPIPDTAQ
TH_1780|M.thermoresistible__bu GDPAVGGDECHMAFHFPLMPRIFMAVR-RESRFPISEILAQTPDIPETAQ
Mb0131|M.bovis_AF2122/97 GDPNTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEIIAQTPPIPDMAQ
Rv0126|M.tuberculosis_H37Rv GDPNTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEIIAQTPPIPDMAQ
MMAR_0325|M.marinum_M GEASTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEILAKTPPIPDMAQ
MUL_4797|M.ulcerans_Agy99 GEASTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEILAKTPPIPDMAQ
MAV_5186|M.avium_104 GDPSTGGDECHMAFHFPLMPRIFMAVR-RESRFPISEILAQTPQIPEMAQ
Mflv_1577|M.gilvum_PYR-GCK DIREIGEAGADLSYDFITRPGVQHALATADTEFLRLTLRTTLELGVDPAS
Mvan_5178|M.vanbaalenii_PYR-1 DIREIGEAGADLSYDFITRPAAHHALATADTEFLRLTLRTTLELGVDPAS
. * ..:::.* * *: ::.* : : : *.
MSMEG_6515|M.smegmatis_MC2_155 WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
TH_1780|M.thermoresistible__bu WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
Mb0131|M.bovis_AF2122/97 WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
Rv0126|M.tuberculosis_H37Rv WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
MMAR_0325|M.marinum_M WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
MUL_4797|M.ulcerans_Agy99 WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
MAV_5186|M.avium_104 WGIFLRNHDELTLEMVTDEE--RDYMYAEYAKDPRMKAN-VGIRRRLAPL
Mflv_1577|M.gilvum_PYR-GCK LVHALQNHDELTYELVHWSNGHRDDLFPYKGEEVTGEVLGDIVRTDLREK
Mvan_5178|M.vanbaalenii_PYR-1 LVHALQNHDELTYELVHWSTGHRDDVYTYKGEEITGEVLGETIRSDLSEK
*:****** *:* . ** ::. .:: :. :* *
MSMEG_6515|M.smegmatis_MC2_155 LENDRNQIELFTALLLSLPGSPVLYYGDEIGMGDIIWLGDRDSVRTPMQW
TH_1780|M.thermoresistible__bu LENDRNQIELFTALLLSLPGSPVIYYGDEIGMGDIIWLGDRDAVRTPMQW
Mb0131|M.bovis_AF2122/97 LDNDRNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRIPMQW
Rv0126|M.tuberculosis_H37Rv LDNDRNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRIPMQW
MMAR_0325|M.marinum_M LENDRNQIQLFNALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRTPMQW
MUL_4797|M.ulcerans_Agy99 LENDRNQIQLFNALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRTPMQW
MAV_5186|M.avium_104 LDNDRNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRTPMQW
Mflv_1577|M.gilvum_PYR-GCK LSGDNAPYNLVFTTNG-IACTSATIIAATLGITSLDDITEPEQIDR-IRR
Mvan_5178|M.vanbaalenii_PYR-1 LTGENAPYNLVFTTNG-IACTTATVIAATLGIAALDDIVDDEQIDR-IRR
* .:. :*. : :. :.. . :*: : : : : : ::
MSMEG_6515|M.smegmatis_MC2_155 TPDRNAGFSKATP-----------GRLYLPPN--------------QDAV
TH_1780|M.thermoresistible__bu TPDRNAGFSKANP-----------GRLYLPVN--------------QDPI
Mb0131|M.bovis_AF2122/97 TPDRNAGFSTANP-----------GRLYLPPS--------------QDPV
Rv0126|M.tuberculosis_H37Rv TPDRNAGFSTANP-----------GRLYLPPS--------------QDPV
MMAR_0325|M.marinum_M TPDRNAGFSTANP-----------GRLYLPPS--------------QDSV
MUL_4797|M.ulcerans_Agy99 TPDRNAGFSTANP-----------GRLYLPPS--------------QDSV
MAV_5186|M.avium_104 TPDRNAGFSKANP-----------GRLYLPPS--------------QDPV
Mflv_1577|M.gilvum_PYR-GCK AHLLLAMFNALQPGVFALSGWDVCGMLTLPSSTIPELLREGDTRWIHRAA
Mvan_5178|M.vanbaalenii_PYR-1 AHLLLAMFNALQPGVFALSGWDVCGMLTLPPSQITELLHGGDTRWIHRAA
: * *. * * * ** . : .
MSMEG_6515|M.smegmatis_MC2_155 YGYHSVNVEAQLDSS-------------------SSLLNWTRNMLAVRSR
TH_1780|M.thermoresistible__bu YGYQSVNVEAQRDSS-------------------TSLLNWTRKMLAVRRR
Mb0131|M.bovis_AF2122/97 YGYQAVNVEAQRDTS-------------------TSLLNFTRTMLAVRRR
Rv0126|M.tuberculosis_H37Rv YGYQAVNVEAQRDTS-------------------TSLLNFTRTMLAVRRR
MMAR_0325|M.marinum_M YGYQAVNVEAQRDTS-------------------TSLLNWTRTMLAVRRR
MUL_4797|M.ulcerans_Agy99 YGYQAVNVEAQRDTS-------------------TSLLNWTRTMLAVRRR
MAV_5186|M.avium_104 YGYQAVNVEAQRDTS-------------------TSLLNFTRTMLAVRRR
Mflv_1577|M.gilvum_PYR-GCK HDLMGVNPEATQSPAGMPRGRSLYGSIPEQLADDTSFLRQLQAILKVRSH
Mvan_5178|M.vanbaalenii_PYR-1 HDLMGVNPTATRSPAGMPRGRSLYGSIPDQLAEDTSFLRQLQAILKVRSH
:. .** * ..: :*:*. : :* ** :
MSMEG_6515|M.smegmatis_MC2_155 HDAFAVGTFRELGGSNPSVLAYIREVTRQQGDGGAKTDAVLCVNNLSRFP
TH_1780|M.thermoresistible__bu HRAFAVGDFRELGGSNPSVLAFIRELEE---DG--QKDTVLCVNNLSRFP
Mb0131|M.bovis_AF2122/97 HPAFAVGAFQELGGSNPSVLAYVRQVAG------DDGDTVLCVNNLSRFP
Rv0126|M.tuberculosis_H37Rv HPAFAVGAFQELGGSNPSVLAYVRQVAG------DDGDTVLCVNNLSRFP
MMAR_0325|M.marinum_M HNAFAVGTFQELGGSNPSILAYVREAPGP---AGEDGDVVLCVNNLSRFP
MUL_4797|M.ulcerans_Agy99 HNAFAVGTFQELGGSNPSILAYVREAPGP---AGEDGDVVLCVNNLSRFP
MAV_5186|M.avium_104 HEAFAIGTFEELGGSNPSVLAFVRQVSN-------DGDTVLCVNNLSRFP
Mflv_1577|M.gilvum_PYR-GCK YGIATSRQIDIPEVSQRGMLVLVHHLDTD------NQYQLTVLNFANEDV
Mvan_5178|M.vanbaalenii_PYR-1 YGIATSRQIDIPEVSHRGMLVLVHQLHEE------GRYQLTVLNFANEDV
: : : *: .:*. ::. : :* ..
MSMEG_6515|M.smegmatis_MC2_155 QPIELNLQQWAGYIPVEMTGYVEFPSIGQLP-YLLTLPGHGFYWFQLREP
TH_1780|M.thermoresistible__bu QPIELQLQNWSGYTPVEMTGYVEFPRIGQLP-YLLTLPGHGFYWFQLREP
Mb0131|M.bovis_AF2122/97 QPIELDLQQWTNYTPVELTGHVEFPRIGQVP-YLLTLPGHGFYWFQLTTH
Rv0126|M.tuberculosis_H37Rv QPIELDLQQWTNYTPVELTGHVEFPRIGQVP-YLLTLPGHGFYWFQLTTH
MMAR_0325|M.marinum_M QPIELNLQQWNSYTPVELTGQVEFPKIGHLP-YLLTLPGHGFYWFQLSAS
MUL_4797|M.ulcerans_Agy99 QPIELNLQQWNSYTPVELTGQVEFRKIGHLP-YLLTLPGHGFYWFQLSAS
MAV_5186|M.avium_104 QPIELNLQHWSGCIPVELTGHVEFPRIGHLP-YLLTLPGHGFYWFQLTAC
Mflv_1577|M.gilvum_PYR-GCK TGTVRSKKLPPGSEVSDMFSGRHLATVDDLHSFSVELTAHQGLSLLVEAP
Mvan_5178|M.vanbaalenii_PYR-1 AGTVRSLKLPPGSEVSDMFSGRPLATVDDLHSFGVELTAHQGLSLLVEAP
. : . :: . : :..: : : *..* : :
MSMEG_6515|M.smegmatis_MC2_155 DPEP-----GAQQ-
TH_1780|M.thermoresistible__bu EPEV-----GEQP-
Mb0131|M.bovis_AF2122/97 EVGAPPTCGGERRL
Rv0126|M.tuberculosis_H37Rv EVGAPPTCGGERRL
MMAR_0325|M.marinum_M EE--------ER--
MUL_4797|M.ulcerans_Agy99 EE--------ER--
MAV_5186|M.avium_104 EEDT----------
Mflv_1577|M.gilvum_PYR-GCK AEDTDAD-------
Mvan_5178|M.vanbaalenii_PYR-1 VPDAGTEGDHVQ--