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NTLVELAGGIVVGVGTPSDAATFTLTDEDTDDRRAVRVASVDEALAELTERCLRWPQAAAVCDDVLRAFD PQGCTFTGVITESLAYSTLQAGAEFARWLDARGPARVPEIADPVVAERVDDVLTIRFNRPQRHNAFSTDA RAALLDALEVARLDPSVTGMVLTGNGPSFCSGGDLAEFGTFTDPASAHLARTRYSPALVLAEITARLGDK CRAEIHGQVQGSGLEMAAFCGTVSCDAGAVLGLPELALGLIPGAGGTVSITRRIGRWRTAYLVLSGRSID AATALRWGLVDAVG*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0448 | - | - | 100% (295) | enoyl-CoA hydratase/isomerase |
| M. gilvum PYR-GCK | Mflv_2117 | - | 7e-22 | 34.05% (185) | enoyl-CoA hydratase/isomerase |
| M. gilvum PYR-GCK | Mflv_2036 | - | 4e-19 | 31.87% (182) | enoyl-CoA hydratase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1099c | echA8 | 2e-20 | 32.61% (184) | enoyl-CoA hydratase |
| M. tuberculosis H37Rv | Rv1070c | echA8 | 2e-20 | 32.61% (184) | enoyl-CoA hydratase |
| M. leprae Br4923 | MLBr_02402 | - | 6e-21 | 33.15% (184) | enoyl-CoA hydratase |
| M. abscessus ATCC 19977 | MAB_1185c | - | 2e-19 | 29.84% (191) | enoyl-CoA hydratase |
| M. marinum M | MMAR_0453 | echA8_4 | 1e-118 | 71.62% (296) | enoyl-CoA hydratase, EchA8_4 |
| M. avium 104 | MAV_4960 | - | 1e-120 | 74.49% (294) | enoyl-CoA hydratase/isomerase family protein |
| M. smegmatis MC2 155 | MSMEG_0259 | - | 1e-120 | 73.47% (294) | enoyl-CoA hydratase/isomerase |
| M. thermoresistible (build 8) | TH_4433 | - | 1e-122 | 73.97% (292) | PUTATIVE enoyl-CoA hydratase/isomerase |
| M. ulcerans Agy99 | MUL_1103 | echA8_4 | 1e-114 | 69.93% (296) | enoyl-CoA hydratase, EchA8_4 |
| M. vanbaalenii PYR-1 | Mvan_0219 | - | 1e-140 | 84.07% (295) | enoyl-CoA hydratase/isomerase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0448|M.gilvum_PYR-GCK MNTLVELA--------------GGIVVGVG---TPSDA------ATFTLT
Mvan_0219|M.vanbaalenii_PYR-1 MNSPVELG--------------GGVRVAVG---TPSDD------ATFTVT
MSMEG_0259|M.smegmatis_MC2_155 ---MIELP--------------GGIRIGLIDDPDDAED------ATFTLS
TH_4433|M.thermoresistible__bu -VSILQLA--------------DGVVIGLG---EPVDD------ATFTLT
MMAR_0453|M.marinum_M MFGVVDLSRAPDATRAAGEISAPGVTVAHGWASELAEAEPWLSAATFTLA
MUL_1103|M.ulcerans_Agy99 MFGVVDLSRAPDATRAAGEISAPGVTVAHGWASELAEAEPWLSAATFTLA
MAV_4960|M.avium_104 MLRVVDLSSAPQD----GPASPPGVIVAVGSADQIARSEFWLDAATFTLT
Mb1099c|M.bovis_AF2122/97 --------------------------------------------MTYETI
Rv1070c|M.tuberculosis_H37Rv --------------------------------------------MTYETI
MLBr_02402|M.leprae_Br4923 --------------------------------------------MKYDTI
MAB_1185c|M.abscessus_ATCC_199 -----------------------------------------MTSHNFETI
.:
Mflv_0448|M.gilvum_PYR-GCK DEDTDDRRAVRVASVDEALAELTERCLRWPQAAAVCDDVLRAFDPQGCTF
Mvan_0219|M.vanbaalenii_PYR-1 EDDVDDRRAVTVTSVPDTLAELEERCRRWPQAAAVCDDVLRAFDPGGSTF
MSMEG_0259|M.smegmatis_MC2_155 EAATDDRRVVTVASIADTLELLRQRCERRPQTAAVCDDVLRSADPDASTF
TH_4433|M.thermoresistible__bu EKPTDDRRAVTVESVQDTLDLLRERCGKWPQTAAVCDDVLRSIDPDGPTR
MMAR_0453|M.marinum_M EGPCADRRVITVESVSQTLAELRQRCQRWPQASAVCDDVLRTIDPDGPTL
MUL_1103|M.ulcerans_Agy99 EGPCADRRVITVESVSQTLAELRQRVPRWPQARAVCDDVLRTIDPDGSTL
MAV_4960|M.avium_104 EDACTDRRAVTVDSVSTSLDELTARCGRWPHASAICDDVLRAVDPAGPAA
Mb1099c|M.bovis_AF2122/97 LVERDQRVGIITLNRPQALNALNSQVMN--------EVTSAATELDDDPD
Rv1070c|M.tuberculosis_H37Rv LVERDQRVGIITLNRPQALNALNSQVMN--------EVTSAATELDDDPD
MLBr_02402|M.leprae_Br4923 LVDGYQRVGIITLNRPQALNALNSQMMN--------EITNAAKELDIDPD
MAB_1185c|M.abscessus_ATCC_199 LTERIDRVAVITLNRPKALNALNSQVMN--------EVTTAAAEFDADHG
:* : . . :* * : . : . : :
Mflv_0448|M.gilvum_PYR-GCK TGVITESLAYSTLQAGAEFARWLDARGPARVPEIADPVVAERVDDVLTIR
Mvan_0219|M.vanbaalenii_PYR-1 AGVITESLAYSTLQSGSEFARWLAERGPARVPDISDPVVAERHDGRLTIR
MSMEG_0259|M.smegmatis_MC2_155 SGVITESLAYSTLQSGAEFARWLDERGPATVPQLPDPVRAHRDGNTLHVR
TH_4433|M.thermoresistible__bu SGVITESLAYSTLQSGSEFARWLAERGPKTVPQLPDPVLATRDGDTLRVV
MMAR_0453|M.marinum_M AGVLTESLAYSTLQAGPEFARWLDERGPADLPDIADPVQTERDDDTLRIR
MUL_1103|M.ulcerans_Agy99 AGVLTESLAYSTLQAGPEFARWLDERGPADLPDIADPVQTERDDDTLRIR
MAV_4960|M.avium_104 RGLLTESLAYSTLQAGPEFARWLDERGPARMPEIADPVLARRDGDVLRIA
Mb1099c|M.bovis_AF2122/97 IGAIIITGSAKAFAAG------------ADIKEMADLTFADAFTADFFAT
Rv1070c|M.tuberculosis_H37Rv IGAIIITGSAKAFAAG------------ADIKEMADLTFADAFTADFFAT
MLBr_02402|M.leprae_Br4923 VGAILITGSPKVFAAG------------ADIKEMASLTFTDAFDADFFSA
MAB_1185c|M.abscessus_ATCC_199 IGAIIITGSEKAFAAG------------ADIKEMSEQSFSDMFGSDFFSA
* : : : ..: :* : ::.. : :
Mflv_0448|M.gilvum_PYR-GCK FNR--PQRHNAFSTDARAALLDALEVARLDPSVTGMVLTGNGPSFCSGGD
Mvan_0219|M.vanbaalenii_PYR-1 FNR--PQRHNAFSTDARAALLEALEVARLDPSVDEVVLAGNGPSFCSGGD
MSMEG_0259|M.smegmatis_MC2_155 FNR--PQRHNAFSTDARAALLEALEVARLDPSVDEVVLAGNGQSFCSGGD
TH_4433|M.thermoresistible__bu FNR--PQRHNAFSTDARAALLEALEVARLDPSVTEVVLTGNGPSFGSGGD
MMAR_0453|M.marinum_M FNR--PQRHNAFSGDARAALLEALTVAQLDPSVSGIVLSGNGPSFCSGGD
MUL_1103|M.ulcerans_Agy99 FNR--PQRHIAFSGDARAALLEALTVAQLDPSVSGIVLSGNGPSFCSGGD
MAV_4960|M.avium_104 FNR--PQRHNAFSTDARAALLEALAVAQLDPSVTGIVLSGNGPSFCSGGD
Mb1099c|M.bovis_AF2122/97 WGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGV
Rv1070c|M.tuberculosis_H37Rv WGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGV
MLBr_02402|M.leprae_Br4923 WGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGV
MAB_1185c|M.abscessus_ATCC_199 WGKLGAVRTPTIAAVSGYALGGGCELAMMCDVIIAAENATFGQPEIKLGV
:.: . * :: : ** . :* : : : * . . *
Mflv_0448|M.gilvum_PYR-GCK LAEFGTFTDPASAHLARTRYSPALVLAEITARLGDKCRAEIHGQVQGSGL
Mvan_0219|M.vanbaalenii_PYR-1 LAEFGTFTDPAGAHLARTRHSPALVLDEITARLGRGCRAELHGQVQGSGL
MSMEG_0259|M.smegmatis_MC2_155 LAEFGSFADPAAAHLARTRYSPALVLDELTARLGRRCRAHVHGQVLGSGL
TH_4433|M.thermoresistible__bu LAEFGSFDDPAGAHLARTRHSPALVLAELTERLGPRCRAEVHGLVLGSGL
MMAR_0453|M.marinum_M LAEFGTFADPASAHLARTRHSPALALAALTARLGRACRAEVHGRVLGSGL
MUL_1103|M.ulcerans_Agy99 LAELGTFADPASAHLARTRHSPALALAALTARLGRACRAEVHGRVLGSGL
MAV_4960|M.avium_104 LAEFGTFADPASAHLARTRHSPALVLDALTARFGRACRAEVHGRVLGSGL
Mb1099c|M.bovis_AF2122/97 LPGMG--GSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD
Rv1070c|M.tuberculosis_H37Rv LPGMG--GSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD
MLBr_02402|M.leprae_Br4923 LPGMG--GSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVVLADD
MAB_1185c|M.abscessus_ATCC_199 LPGMG--GSQRLTRAIGKAKAMDMILTGRNMDAAEAERSGLVSRVVATES
*. :* . :: . : : * . . *: : . * :
Mflv_0448|M.gilvum_PYR-GCK EMAAFCGTVSCDAGAVLGLPELALGLIPGAGGTVSITRRIGRWRTAYLVL
Mvan_0219|M.vanbaalenii_PYR-1 EMAAFCGWVSCDADAVLGLPELTLGLIPGAGGTVSVTRRIGRWRTAYLVL
MSMEG_0259|M.smegmatis_MC2_155 EMACFCGWVSCDPDAKLGLPELELGLVPGAGGTVSITRRIGRWRTAYLVL
TH_4433|M.thermoresistible__bu EMASFCGHVTAHPDAVFGLPELELGLIPGAGGCVSITRRIGRWRTAYLVL
MMAR_0453|M.marinum_M EMAAFCGWVQAHPDSVFGLPELSLGLIPGAGGTVSVTRRIGRWRTAYLVL
MUL_1103|M.ulcerans_Agy99 EMAAFCGWVQAQPDSVFGLPELSLGLIPGAGGAVSVTRRIGRWRTAYLAL
MAV_4960|M.avium_104 EMAAFCGWVSARDDAVFGLPELALGLIPGAGGTVSITRRIGRWRTAYLVL
Mb1099c|M.bovis_AF2122/97 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF
Rv1070c|M.tuberculosis_H37Rv LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF
MLBr_02402|M.leprae_Br4923 LLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTF
MAB_1185c|M.abscessus_ATCC_199 LLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAF
: . . . . : : : : . * : .:
Mflv_0448|M.gilvum_PYR-GCK SGRSIDAATALRWGLVDAVG------
Mvan_0219|M.vanbaalenii_PYR-1 SGRPIDAGTALRWGLVDAVG------
MSMEG_0259|M.smegmatis_MC2_155 SGRTIDPTTALSWGLVDEIVSVAAPA
TH_4433|M.thermoresistible__bu TGRRIDAVTALRWGLVDAVSS-----
MMAR_0453|M.marinum_M SGRTIDAGTARDWGLVDSIAVTGD--
MUL_1103|M.ulcerans_Agy99 SGRTIDAGTARDWGLVDSIAVTGD--
MAV_4960|M.avium_104 SGRTVGAATALSWGLVDEVEAATASS
Mb1099c|M.bovis_AF2122/97 ATEDQSEGMAAFIEKRAPQFTHR---
Rv1070c|M.tuberculosis_H37Rv ATEDQSEGMAAFIEKRAPQFTHR---
MLBr_02402|M.leprae_Br4923 VTDDQSEGMAAFIEKRAPQFTHR---
MAB_1185c|M.abscessus_ATCC_199 GTADQSEGMAAFVEKRPANFIHR---
. *