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SDATADSREGSQFGPYRLRRIVGRGAMGDVYEAEDTVRERVVALKLMSQTLSNDPVFRTRMQREARTAGR LQEPHVVPIHDYGEIDGQLYVDMRLIDGKDLAAMLKRYGPLPPPRAVAITRQIGSALDAAHAAGVTHRDV KPENILVSHDDFAYLVDFGIASATSDEKLTQLGSTVGTLYYMAPERFSSGDVTYRADIYALACVLYECLT GSPPYRGDQLSVIGAHLSQPIPRPSAARPGIPAGFDNVIARGMAKDPADRYATCGDLSAAAYSALATPDQ DRATDILQRSQVAQLPAGFASTSPGGFAPSSPAVPVPGSAPSTGWPAPSVPGAPVAPSAPVPTPWAGVPG WGVGHTGTTGAPPWGQPPPEPTVAAKTWPWIAAAVAVVVVIAGVLGVVIAHPWRSPGPPTASAPPPPPPD AVQLRVLNDGVFIGSSAATTTIDIFNEPICPPCGAFIRSYASDIDAAVANKKLAVRYHLLNFLDEQSHTK TYSTRAVAASYCVAAQDDPKVYTDFYAALFASDFQPQEAAASDRTDAELAHLAQTVGANGTATSCIKAGN DMGTARTKAAAADATLSELNAGLRRC*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. ulcerans Agy99 | MUL_3180 | pknE | - | 100% (587) | transmembrane serine/threonine-protein kinase E PknE |
| M. ulcerans Agy99 | MUL_3375 | pknH_2 | e-119 | 50.62% (486) | transmembrane serine/threonine-protein kinase H PknH_2 |
| M. ulcerans Agy99 | MUL_4471 | pknF | 2e-53 | 35.17% (418) | anchored-membrane serine/threonine-protein kinase PknF |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1772 | pknE | 0.0 | 74.01% (581) | transmembrane serine/threonine-protein kinase E |
| M. gilvum PYR-GCK | Mflv_2026 | - | 1e-119 | 52.12% (472) | protein kinase |
| M. tuberculosis H37Rv | Rv1743 | pknE | 0.0 | 74.18% (581) | transmembrane serine/threonine-protein kinase E |
| M. leprae Br4923 | MLBr_00897 | - | 1e-48 | 38.13% (257) | serine-threonine protein kinase |
| M. abscessus ATCC 19977 | MAB_4435 | - | 1e-53 | 41.45% (304) | serine/threonine-protein kinase PknJ |
| M. marinum M | MMAR_2581 | pknE | 0.0 | 99.48% (582) | transmembrane serine/threonine-protein kinase E PknE |
| M. avium 104 | MAV_1417 | - | 1e-138 | 59.33% (445) | serine/threonine-protein kinase PknE |
| M. smegmatis MC2 155 | MSMEG_4366 | - | 2e-87 | 49.42% (346) | serine/threonine-protein kinase PknD |
| M. thermoresistible (build 8) | TH_2947 | - | 4e-65 | 57.14% (210) | PUTATIVE serine/threonine-protein kinase PknD |
| M. vanbaalenii PYR-1 | Mvan_4693 | - | 1e-122 | 55.82% (421) | protein kinase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2026|M.gilvum_PYR-GCK -----------MTDPQHGSRVGSQVGPYRLQRLLGKGGMGEVYEAYDTVK
Mvan_4693|M.vanbaalenii_PYR-1 -----------MTDPQRGSRVGSQVGPYRLRRLLGKGGMGEVYEAEDTVK
MUL_3180|M.ulcerans_Agy99 -----------MSDATADSREGSQFGPYRLRRIVGRGAMGDVYEAEDTVR
MMAR_2581|M.marinum_M -----------MSDATADSREGSQFGPYRLRRIVGRGAMGDVYEAEDTVR
Mb1772|M.bovis_AF2122/97 ------------MDGTAESREGTQFGPYRLRRLVGRGGMGDVYEAEDTVR
Rv1743|M.tuberculosis_H37Rv ------------MDGTAESREGTQFGPYRLRRLVGRGGMGDVYEAEDTVR
MAV_1417|M.avium_104 ------------MSDERSSKVGSMFGPYHLKRLLGRGGMGEVYEAEHTVK
MSMEG_4366|M.smegmatis_MC2_155 ---------MTPMASNGASRLGTRFGPYELRSLIGVGGMGEVYRAYDTVK
TH_2947|M.thermoresistible__bu -------------MSGPESRAGTRFGKYELTALLGRGGMGEVYEAFDTEK
MLBr_00897|M.leprae_Br4923 ----------------------MLDGRYLLKAKIGNGGTATVYRGVDIRL
MAB_4435|M.abscessus_ATCC_1997 MTSSDRSSPGPGGQQPGDLPPGTVVSGYQIERVLGRGGMGTVYLAANPNL
. * : :* *. . ** . .
Mflv_2026|M.gilvum_PYR-GCK DRVVALKLLPESASHDPVFRKRLQREAHAAGRLQEPHVVPIHDYG-EIDG
Mvan_4693|M.vanbaalenii_PYR-1 DRVVALKLLPEAVSHDPVFRKRLQREAHAAGRLQEPHVVPIHDYG-EVDG
MUL_3180|M.ulcerans_Agy99 ERVVALKLMSQTLSNDPVFRTRMQREARTAGRLQEPHVVPIHDYG-EIDG
MMAR_2581|M.marinum_M ERVVALKLMSQTLSNDPVFRTRMQREARTAGRLQEPHVVPIHDYG-EIDG
Mb1772|M.bovis_AF2122/97 ERIVALKLMSETLSSDPDFRTRMQREARTAGRLQEPHVVPIHDFG-EIDG
Rv1743|M.tuberculosis_H37Rv ERIVALKLMSETLSSDPVFRTRMQREARTAGRLQEPHVVPIHDFG-EIDG
MAV_1417|M.avium_104 EWTVAVKLLNESFSSDPVFRERMKREARTAGRLQEPHVVPVHDYG-EIDG
MSMEG_4366|M.smegmatis_MC2_155 GREVAVKLLRAEFAADPRFQERFRRESRVAARLQEPHVIPVHDFG-DIDG
TH_2947|M.thermoresistible__bu RRAVALKILRAEYARDDRFRTRFLRESHAAAILQEPHVIPIHDWG-EIDG
MLBr_00897|M.leprae_Br4923 DRPVAVKVMDSRYTGDEQLLTRFQLEARSVARLKDPGLVAVYDQG-IDAR
MAB_4435|M.abscessus_ATCC_1997 QRSEALKILSAQSSRDPEFRARFMREASLAASLDHPNIVTVHNRGETPEG
*:*:: : * : *: *: .. *..* ::.::: *
Mflv_2026|M.gilvum_PYR-GCK LLFVDMRMIDGVDLRRILKEQGAMSPARATAIVRQIASALDAAHRAGIMH
Mvan_4693|M.vanbaalenii_PYR-1 LLFVDMRLIDGTDLRKLLKEQGPMTPARATAIVAQVASALDAAHQNGIMH
MUL_3180|M.ulcerans_Agy99 QLYVDMRLIDGKDLAAMLKRYGPLPPPRAVAITRQIGSALDAAHAAGVTH
MMAR_2581|M.marinum_M QLYVDMRLIDGKDLAAMLKRYGPLPPPRAVAITRQIGSALDAAHAAGVTH
Mb1772|M.bovis_AF2122/97 QLYVDMRLINGVDLAAMLRRQGPLAPPRAVAIVRQIGSALDAAHAAGATH
Rv1743|M.tuberculosis_H37Rv QLYVDMRLINGVDLAAMLRRQGPLAPPRAVAIVRQIGSALDAAHAAGATH
MAV_1417|M.avium_104 QMFLEMRLVEGTDLDSVLKRFGPLPPPRAVAIITQIASALDAAHAAGVMH
MSMEG_4366|M.smegmatis_MC2_155 VLYIDMRLVEGASLKDILRTDGPLDPRRTASIVAQVASALDAAHADGLVH
TH_2947|M.thermoresistible__bu NLFIDMRLVQGRTLHDLI-TDGPLGPDRAVEIVQQVADALDAAHAEGLVH
MLBr_00897|M.leprae_Br4923 HPFLVMELIEGGTLRELLAERGPMPPHAVVAVLRPVLGGLAAAHRAGLVH
MAB_4435|M.abscessus_ATCC_1997 DLWIAMQYVQGSDAHSES-ASGRMSLTRAVHIISEVAKALDYLHARGLVH
:: *. ::* * : .. : : .* * * *
Mflv_2026|M.gilvum_PYR-GCK RDVKPENILVTRDDF-AYLVDFGIANAATDE-SLTELGTAVGTYAYMAPE
Mvan_4693|M.vanbaalenii_PYR-1 RDVKPENILINRDDF-AYLVDFGIANAVTDE-ALTELGTAVGTYAYMAPE
MUL_3180|M.ulcerans_Agy99 RDVKPENILVSHDDF-AYLVDFGIASATSDE-KLTQLGSTVGTLYYMAPE
MMAR_2581|M.marinum_M RDVKPENILVSHDDF-AYLVDFGIASATSDE-KLTQLGSTVGTLYYMAPE
Mb1772|M.bovis_AF2122/97 RDVKPENILVSADDF-AYLVDFGIASATTDE-KLTQLGNTVGTLYYMAPE
Rv1743|M.tuberculosis_H37Rv RDVKPENILVSADDF-AYLVDFGIASATTDE-KLTQLGNTVGTLYYMAPE
MAV_1417|M.avium_104 RDVKPQNILVTRDDF-AYLVDFGIASATTDE-KLTQLGTAVGTWKYMAPE
MSMEG_4366|M.smegmatis_MC2_155 RDIKPENVLLTGDDF-AYLVDFGIAHVGGEA-SVTMTGMLIGSSAYMAPE
TH_2947|M.thermoresistible__bu RDVKPHNILVTPADF-AYLVDFGIAAMQGDS-RLTLAGTQIGSLAYMAPE
MLBr_00897|M.leprae_Br4923 RDVKPENILISDDGD-VKIADFGLVRAVAAA-GITSTSVILGTVAYLSPE
MAB_4435|M.abscessus_ATCC_1997 RDVKPANILLSAGDRRIMLGDFGLTRALDDSNSVTSAGNVTATIAYAAPE
**:** *:*:. . : ***:. :* . .: * :**
Mflv_2026|M.gilvum_PYR-GCK RFAAGEVTHRADVYALTCVLHECLTGTQPFE-GDSVSVVITAHLNQPAPR
Mvan_4693|M.vanbaalenii_PYR-1 RFTNGEVTHRADVYALTCVLHECLTGAQPFQ-GDSVSVVITAHLNDPAPR
MUL_3180|M.ulcerans_Agy99 RFSSGDVTYRADIYALACVLYECLTGSPPYR-GDQLSVIG-AHLSQPIPR
MMAR_2581|M.marinum_M RFSSGDVTYRADIYALACVLYECLTGSPPYR-GDQLSVMG-AHLSQPIPR
Mb1772|M.bovis_AF2122/97 RFSESHATYRADIYALTCVLYECLTGSPPYQ-GDQLSVMG-AHINQAIPR
Rv1743|M.tuberculosis_H37Rv RFSESHATYRADIYALTCVLYECLTGSPPYQ-GDQLSVMG-AHINQAIPR
MAV_1417|M.avium_104 RFSDAEVTYRADIYALACVLFECLTGSAPYR-ADSAGVLVSAHVMDPIPA
MSMEG_4366|M.smegmatis_MC2_155 RFSGETVGPPADIYALACLVYESLIGRPPYE-AGDLRQLMSAHMFSAPPR
TH_2947|M.thermoresistible__bu RFNDEPCTPATDIYALACVLYEALTGNTPFD-TGSHESLX-----XXPRR
MLBr_00897|M.leprae_Br4923 QVRDGDASPRSDVYSAGIMTYELLTGHTPFT-GDSALSIAYQRLEIDVPR
MAB_4435|M.abscessus_ATCC_1997 ILSGTAVDGRADVYALGCTLFRMLTGQPPFGSGNSLPAIINAHLSAPPPR
. :*:*: .. * * *: .. :
Mflv_2026|M.gilvum_PYR-GCK PSSLRQGIPAGLDEVIARGMAKRPEDRFASAGELADAATDALTRAEQDQA
Mvan_4693|M.vanbaalenii_PYR-1 PSQSRPGIPAGLDDVISRGMAKRPEDRYASAGEMARAATAALTGAQQDQA
MUL_3180|M.ulcerans_Agy99 PSAARPGIPAGFDNVIARGMAKDPADRYATCGDLSAAAYSALATPDQDRA
MMAR_2581|M.marinum_M PSAARPGIPAGFDNVIARGMAKDPADRYATCGDLSAAAYSALATPDQDRA
Mb1772|M.bovis_AF2122/97 PSTVRPGIPVAFDAVIARGMAKNPEDRYVTCGDLSAAAHAALATADQDRA
Rv1743|M.tuberculosis_H37Rv PSTVRPGIPVAFDAVIARGMAKNPEDRYVTCGDLSAAAHAALATADQDRA
MAV_1417|M.avium_104 PSARRPGVPKAFDAVIARGMAKKPEDRYASAGDLALAAKEALSTPDQDRA
MSMEG_4366|M.smegmatis_MC2_155 PSIMRRGVPRAFDEVIATAMAKDPAARYATAGELARAVSAAAAG---EAP
TH_2947|M.thermoresistible__bu HRTPRR--------VVG--------------------AEVDGAG---DQE
MLBr_00897|M.leprae_Br4923 ASTVITGVPQQFDELVARATVRDPSGRYADAIEMAAQVDSIAEELALPPF
MAB_4435|M.abscessus_ATCC_1997 ISQLAP-VPESWDALIAKAMAKTPADRYQSAGELAMAAQQILTDPAQHRQ
::. .
Mflv_2026|M.gilvum_PYR-GCK ASILQRSQAATMMAPLP----PPPPPPPPPPP--------PQFAATPPPG
Mvan_4693|M.vanbaalenii_PYR-1 DSILQRSQAATMMAPLP----PPPPPPPPSTPGGPLPYGGSPVPPPPPPP
MUL_3180|M.ulcerans_Agy99 TDILQRSQVAQLPAGF-----ASTSPGGFAPSSPAVPVPGSAPSTGWPAP
MMAR_2581|M.marinum_M TDILQRSQVAQLPAGF-----ASTPPGGFAPSSPAVPVPGSAPSTGWPAP
Mb1772|M.bovis_AF2122/97 TDILRRSQVAKLPV-------PSTHP--------------VSPGTRWPQP
Rv1743|M.tuberculosis_H37Rv TDILRRSQVAKLPV-------PSTHP--------------VSPGTRWPQP
MAV_1417|M.avium_104 ATILRRSQEAALPAAGQRDAGASTVLAPPPAVNTRQPGSAASQAGRGWTP
MSMEG_4366|M.smegmatis_MC2_155 PSKPTPPQVPGRPPTP-----------------------TRQFSELYPNP
TH_2947|M.thermoresistible__bu PVRRRPGPVTG---------------------------------------
MLBr_00897|M.leprae_Br4923 RVP-----------------------------------------------
MAB_4435|M.abscessus_ATCC_1997 TVTQPVPATTPAPIAAP--------------EPVHAMAPLAMADTGQSML
Mflv_2026|M.gilvum_PYR-GCK GA-----------ATYQGGYQPPPPPGWGPP---PVPPGRPGGSGGSKGP
Mvan_4693|M.vanbaalenii_PYR-1 GQ-----------PTQASGFHTPPPPGGPPPGWPPQPPQSSGGGGGSKGP
MUL_3180|M.ulcerans_Agy99 SVPGAPVAPSAPVPTPWAGVPGWGVGHTGTTGAPPWGQPP-PEPTVAAKT
MMAR_2581|M.marinum_M SVPGAPVAPSAPVPTPWAGVPGWGVGHTGTTGAPPWGQPP-PQPTVAAKT
Mb1772|M.bovis_AF2122/97 --------------TPWAG------------GAPPWGPPSSPLPRSARQP
Rv1743|M.tuberculosis_H37Rv --------------TPWAG------------GAPPWGPPSSPLPRSARQP
MAV_1417|M.avium_104 ESGPIPAAGQPGPPYYQGGNANWAAAPAQFAGPPPWGRPP-----PKRRV
MSMEG_4366|M.smegmatis_MC2_155 E----------------------------HTGFTPYPPAYPSVPAPPRRT
TH_2947|M.thermoresistible__bu -------------------------------------------DVPDRRA
MLBr_00897|M.leprae_Br4923 ---------------------------------------------APRNT
MAB_4435|M.abscessus_ATCC_1997 KHKRIAAIIGVMVLVVASSVVAVLIWPRADEGTDPRTKASPSAAQVAPGA
Mflv_2026|M.gilvum_PYR-GCK LIAVVAG-AVVLLVLAAVGIWLVTKSDDPGSTTASPTSSSAAP-TTSREP
Mvan_4693|M.vanbaalenii_PYR-1 MIALLAGAAVIILVLAGLGIWLVVKPDSTSDPVASRTSTSAAPTTTSRGS
MUL_3180|M.ulcerans_Agy99 WPWIAAAVAVVVVIAGVLGVVIAHPWRSPGPPTASAPPPPPPDAVQLRVL
MMAR_2581|M.marinum_M WPWIAAAVAVVVVIAGVLGVVIAHPWRSPGPPTASAPPPPPPDAVQLRVL
Mb1772|M.bovis_AF2122/97 WLWVGVAVAVVVALAGGLGIALAHPWRSSGPRTSAPPPPPPADAVELRVL
Rv1743|M.tuberculosis_H37Rv WLWVGVAVAVVVALAGGLGIALAHPWRSSGPRTSAPPPPPPADAVELRVL
MAV_1417|M.avium_104 WPIVAAVVAVLVVIAGGVGVWLVIRPK-PEPPQAAPLTSDRLGALLLSAS
MSMEG_4366|M.smegmatis_MC2_155 PTPPAPAAVAAPAVRTPGKKRFTRTQMMLVVATVVLFGIAAVLATMLVGG
TH_2947|M.thermoresistible__bu AR------------------------------------------------
MLBr_00897|M.leprae_Br4923 AQYRSAALHRGHMVERPVEQQVQPVVNAPMPHSTRQFTRRLRDCSAPR--
MAB_4435|M.abscessus_ATCC_1997 SLAGQPNTVLDTLLPGQFDYPEGWEKNPSPTFRSGEFNPAVPPGSATNIG
Mflv_2026|M.gilvum_PYR-GCK TRRTRTTTSAPPATGSYDQQLMAMLSGGHDGSNCEPVSPPAQTAVATVD-
Mvan_4693|M.vanbaalenii_PYR-1 TTRTRTSTSTPPPTESYDQQLMALLSGGHDSSNCEPVDPPAPTILATVD-
MUL_3180|M.ulcerans_Agy99 NDGVFIGSSAATTTIDIFNEPICPPCGAFIRSYASDIDAAVANKKLAVR-
MMAR_2581|M.marinum_M NDGVFIGSSAATTTIDIFNEPICPPCGAFIRSYASDIDAAVANKKLAVR-
Mb1772|M.bovis_AF2122/97 NDGVFVGSSVAPTTIDIFNEPICPPCGSFIRSYASDIDTAVADKQLAVR-
Rv1743|M.tuberculosis_H37Rv NDGVFVGSSVAPTTIDIFNEPICPPCGSFIRSYASDIDTAVADKQLAVR-
MAV_1417|M.avium_104 EVNAVMGSSTMEP-----GKPITATDSSSVTVSVPDCQGALYTTQDPVYA
MSMEG_4366|M.smegmatis_MC2_155 DDNASTG-----------NTGITPPEGATSTSESTTTTTSNRSATANVS-
TH_2947|M.thermoresistible__bu --------------------------------------------------
MLBr_00897|M.leprae_Br4923 ------------------------------RPARPDIDSPEEYEYEPVP-
MAB_4435|M.abscessus_ATCC_1997 GTPVGCNTIDPAWALRAKGRETSTLYLRDRKKPEREILIRVVPAADALST
Mflv_2026|M.gilvum_PYR-GCK -----CTQNTNPGGPAFTRYSLFADRGALDAAFDEAIRVNSELLECPGSG
Mvan_4693|M.vanbaalenii_PYR-1 -----CTQALTPGGPAFTRYSLFEDRGALDAAFEEAIRVNSELVQCPGSG
MUL_3180|M.ulcerans_Agy99 ---YHLLNFLDEQSHTKTYSTRAVAASYCVAAQDDPKVYTDFYAALFASD
MMAR_2581|M.marinum_M ---YHLLNFLDEQSHTKTYSTRAVAASYCVAAQDDPKVYTDFYAALFASD
Mb1772|M.bovis_AF2122/97 ---YHLLNFLDDQSHSKNYSTRAVAASYCVAGQNDPKLYASFYSALFGSD
Rv1743|M.tuberculosis_H37Rv ---YHLLNFLDDQSHSKNYSTRAVAASYCVAGQNDPKLYASFYSALFGSD
MAV_1417|M.avium_104 GSGYSAVNGLVSSEPGDNYDHWVNQAVVLFPSADKAKSFLQTSLQKWKG-
MSMEG_4366|M.smegmatis_MC2_155 -----GTDEQGFVGHTARCDSDATPAALIRTATSLAIICERDSGGYYYRG
TH_2947|M.thermoresistible__bu --------------------------------------------------
MLBr_00897|M.leprae_Br4923 ----------------------DRFAGIAISEFIWARQHARRMVLIWVGV
MAB_4435|M.abscessus_ATCC_1997 DGASDALKRCDAVAYIIPGSSTTWRCSFEFDEPNPVQNGQKELTTTESCY
Mflv_2026|M.gilvum_PYR-GCK VDSP--TTWHYTETPDQVVGQVACGTYNENPDIVWTNDEKLLLADAQSPN
Mvan_4693|M.vanbaalenii_PYR-1 VDSP--TTWHYTETPDQVVGQIACGTYDGNPDLVWTKDDDLLLADAQGPN
MUL_3180|M.ulcerans_Agy99 FQPQEAAASDRTDAELAHLAQTVGANG-TATSCIKAGNDMGTARTKAAAA
MMAR_2581|M.marinum_M FQPQEAAASDRTDAELAHLAQTVGANG-TATSCIKAGNDMGTARTKAAAA
Mb1772|M.bovis_AF2122/97 FQPQENAASDRTDAELAHLAQTVGAEP-TAISCIKSGADLGTAQTKATNA
Rv1743|M.tuberculosis_H37Rv FQPQENAASDRTDAELAHLAQTVGAEP-TAISCIKSGADLGTAQTKATNA
MAV_1417|M.avium_104 CAGQTVTVTNKSKTYRWTFAQVQGAPPKITLMDTQEGADGWECQRALSVA
MSMEG_4366|M.smegmatis_MC2_155 ERLRDGANLKIDDAQRSGDGYVAVNPA--DGARYEVSPEGLTIMSNGHVD
TH_2947|M.thermoresistible__bu --------------------------------------------------
MLBr_00897|M.leprae_Br4923 VVAIAGLVATVAWTIGSHLSGLL---------------------------
MAB_4435|M.abscessus_ATCC_1997 MFPSGANSIRQHVSFNVVRGLLVYILASGASNRNDAAGSLSATALSFATT
Mflv_2026|M.gilvum_PYR-GCK LEDLHDWWLEFG------------------------
Mvan_4693|M.vanbaalenii_PYR-1 LEELHNWWLEFG------------------------
MUL_3180|M.ulcerans_Agy99 DATLSELNAG-------------------LRRC---
MMAR_2581|M.marinum_M DATLSELNASGTPFVWDGSKSIDLQNPSWLTKLIG-
Mb1772|M.bovis_AF2122/97 SETLAGFNASGTPFVWDGSMVVNYQDPSWLARLIG-
Rv1743|M.tuberculosis_H37Rv SETLAGFNASGTPFVWDGSMVVNYQDPSWLARLIG-
MAV_1417|M.avium_104 NNVIVDINACGYRITDQGDQIAD----KIVAKIDNE
MSMEG_4366|M.smegmatis_MC2_155 SSEPPEEYGSK-------------------------
TH_2947|M.thermoresistible__bu ------------------------------------
MLBr_00897|M.leprae_Br4923 ------------------------------------
MAB_4435|M.abscessus_ATCC_1997 MKILRYGR----------------------------