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SNNPTFGVEEEFLLVDPRTGEPIARNKAVAETAAAKGVDLQLELTSCQVETATEVMDNSDDLRKALLRLR RVATDAAETNGARLLAAGLPPTVPHKFPITPTPRYRRIGHRFGMIAHEQGICGCHVHVEVPSRDAAIRVS NRLRPWLHLLLALTANSAIYRGSDSGYATFRSVLWARWPSAGPPPFFDSEAQYDATVAMLEDAGAALDDG MIYWDVRPSNKFPTVEVRVSDVPATVAETVLFATLVRAAVMTATEAEKNGEPVVPLTDYVLKAAYWKSAR DGLDGRTIDLAESHAVAPTVELLTNFVEHLRPALEQLGEYDTVRGELARVIETGNGAMRQRRAFERRREA ADVIDELAAATIAE*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5590 | - | - | 100% (365) | carboxylate-amine ligase |
| M. smegmatis MC2 155 | MSMEG_3326 | - | 2e-57 | 37.88% (359) | carboxylate-amine ligase |
| M. smegmatis MC2 155 | MSMEG_0836 | - | 2e-29 | 29.25% (359) | carboxylate-amine ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0441 | - | 3e-26 | 29.49% (373) | carboxylate-amine ligase |
| M. gilvum PYR-GCK | Mflv_1816 | - | 1e-136 | 66.67% (363) | carboxylate-amine ligase |
| M. tuberculosis H37Rv | Rv0433 | - | 3e-26 | 29.49% (373) | carboxylate-amine ligase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_2558 | - | 1e-138 | 66.30% (362) | carboxylate-amine ligase |
| M. marinum M | MMAR_5020 | gcs2 | 8e-66 | 42.66% (361) | glutamate--cysteine ligase, Gcs2 |
| M. avium 104 | MAV_4721 | - | 9e-27 | 29.70% (367) | carboxylate-amine ligase |
| M. thermoresistible (build 8) | TH_0948 | - | 7e-30 | 30.03% (363) | CONSERVED HYPOTHETICAL PROTEIN |
| M. ulcerans Agy99 | MUL_4094 | gcs2 | 2e-65 | 42.62% (359) | glutamate--cysteine ligase, Gcs2 |
| M. vanbaalenii PYR-1 | Mvan_4929 | - | 1e-136 | 66.03% (365) | carboxylate-amine ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_5590|M.smegmatis_MC2_155 ----------------MSNNPTFGVEEEFLLVDPRTGEPIARNKAVAETA
MAB_2558|M.abscessus_ATCC_1997 ----------------MDGHPTVGVEEEFLLVDRNTGEPVGRNRAVAQVA
Mflv_1816|M.gilvum_PYR-GCK ----------------MTEIPTLGVEEEFLLVDPESGAPVALNREVADLA
Mvan_4929|M.vanbaalenii_PYR-1 ----------------MTDIPTLGVEEEFLLVHPGSGAPVALNREVAARA
MMAR_5020|M.marinum_M --------------MRRDPPRTMGVEEEFHLVDLRSRRLTTR---APDLL
MUL_4094|M.ulcerans_Agy99 ----------------------MGVEEEFHLVDLRSRRLTTR---APDLL
Mb0441|M.bovis_AF2122/97 ---MPARRSAARIDFAGSPRPTLGVEWEFALVDSQTRDLSNEATAVIAEI
Rv0433|M.tuberculosis_H37Rv ---MPARRSAARIDFAGSPRPTLGVEWEFALVDSQTRDLSNEATAVIAEI
MAV_4721|M.avium_104 MSSVPARRGDAHIDFARSPRPTIGVEWEFALVDAQTRDLSNEATAVIAEI
TH_0948|M.thermoresistible__bu ---VSSPAGSTRIDFAGSPRPTLGVEWEFALVDADTRDLSNEAAEVIAEI
.*** ** **. : .
MSMEG_5590|M.smegmatis_MC2_155 AAKGVDLQLELTSCQVETATEVMDNSDDLRKALLRLRRVATDAAETNGAR
MAB_2558|M.abscessus_ATCC_1997 STHGVDLQLELTSCQVETTTAVMCTSSALRTELIRLRGIASAAAASQRAH
Mflv_1816|M.gilvum_PYR-GCK ARDGVELQLELTRCQVETATGVVDTTVDLREQLVHLRRTAAHAAENAGAR
Mvan_4929|M.vanbaalenii_PYR-1 ADMDVELQLELTSCQVETATAVVDDTAHLREQLLHLRRTASAAAEHAGAR
MMAR_5020|M.marinum_M AKLPEDYVAELQSCVVETNSGIVDSLDALRADLLRHRRLLVDAADEIGVG
MUL_4094|M.ulcerans_Agy99 AKLPEDYVAELQSCVVETNSGIVDSLDALRADLLRHRRLLVDAADEIGVG
Mb0441|M.bovis_AF2122/97 GEN-PRVHKELLRNTVEIVSGICECTAEAMQDLRDTLGPARQIVRDRGME
Rv0433|M.tuberculosis_H37Rv GEN-PRVHKELLRNTVEIVSGICECTAEAMQDLRDTLGPARQIVRDRGME
MAV_4721|M.avium_104 GEN-PRVHKELLRNTVEVVSGICRTVPEAMEDLRQTLGPARRIVRDRGME
TH_0948|M.thermoresistible__bu GEN-PHVHKELLRNTVEFVTGICADTPEAIADLRATLLPARKIVRDRGME
. ** ** : : * .
MSMEG_5590|M.smegmatis_MC2_155 LLAAG-LPPTVPHKFPITPTPRYRRIGHRFGMIAHEQGICGCHVHVEVPS
MAB_2558|M.abscessus_ATCC_1997 LLAVA-LPPTVPHAFPVTPTARYRRMADEFGMLAHEQGICGCHVHVAVPS
Mflv_1816|M.gilvum_PYR-GCK LLAVG-LPPTLPHEFPITDTPRYRDIGERYGMIAHEQGICGCHVHVAVPD
Mvan_4929|M.vanbaalenii_PYR-1 LLAVG-LPPTLPHEFPVTDTPRYRDIGERYGMIAHEQGICGCHVHVAVPD
MMAR_5020|M.marinum_M VVAAGAVPLSVPAELQITQTPRYLHMLADYQLLAREQLICATHVHVGIDD
MUL_4094|M.ulcerans_Agy99 VVAAGAVPLSVPAELQITQTPRYLHMLADYQLLAREQLICATHVHVGIDD
Mb0441|M.bovis_AF2122/97 LFCAGTHPFARWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGIRS
Rv0433|M.tuberculosis_H37Rv LFCAGTHPFARWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGIRS
MAV_4721|M.avium_104 LFCAGAHPFAQWTTQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGISS
TH_0948|M.thermoresistible__bu LFCAGTHPFAKWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGVSS
:.... * : :* :.** : .:: * . **** : .
MSMEG_5590|M.smegmatis_MC2_155 RDAAIRVSNRLRPWLHLLLALTANSAIYR-GSDSGYATFRSVLWARWPSA
MAB_2558|M.abscessus_ATCC_1997 RESAIDVSNRLRPTLHLFLALTANSAVYR-KADSGYASYRNMLWTRWPSS
Mflv_1816|M.gilvum_PYR-GCK RDTAIRVGNRLRPWLPLLLGLTANSAIYR-NADSGHASWRSVLWARWPGA
Mvan_4929|M.vanbaalenii_PYR-1 RDAAIAVSNRLRPWLPLLLALTANSAIYR-NADTGHASWRSVLWARWPSA
MMAR_5020|M.marinum_M RDEAIAIANRLLPCLPTLLALSASSPFWADGSDTGYASMRTLVWRRWPTT
MUL_4094|M.ulcerans_Agy99 RDEAIAIANRLLPCLPTLLALSASSPFWADGSDTGYASMRTLVWRRWPTT
Mb0441|M.bovis_AF2122/97 AHKVMPIMTSLLNYYPHLLALSASSPWWG-GEDTGYASNRAMMFQQLPTA
Rv0433|M.tuberculosis_H37Rv AHKVMPIMTSLLNYYPHLLALSASSPWWG-GEDTGYASNRAMMFQQLPTA
MAV_4721|M.avium_104 PNKVMPIMTSLLNYYPHLLALSASSPWWT-GVDTGYASNRAMMFQQLPTA
TH_0948|M.thermoresistible__bu AHKVMPIISSLLNQYPHLLALSASSPYWD-GEDTGYASNRAMMFQQLPTA
. .: : . * :*.*:*.*. : *:*:*: * ::: : * :
MSMEG_5590|M.smegmatis_MC2_155 GPPPFFDSEAQYDATVAMLEDAGAALDDGMIYWDVRPSNKFPTVEVRVSD
MAB_2558|M.abscessus_ATCC_1997 GPPPYFDSVDHYDTTVRELSRSGAVLDDGMVYWDVRPSANFPTVEVRVAD
Mflv_1816|M.gilvum_PYR-GCK GPPPQFESADEYDAAVQMLCDTNVIRDQGMVYWDVRPSATFPTVEVRVSD
Mvan_4929|M.vanbaalenii_PYR-1 GPPPHFDSADEYDAAVQMLSHTGVIRDDGMVYWDVRPSANFPTVEVRVAD
MMAR_5020|M.marinum_M GLAAPVQSATEYDALVKALIASQVITDYGMIYFDLRPSSHAPTLELRVCD
MUL_4094|M.ulcerans_Agy99 GLAAPVQSATEYDALVKALIASQVITDYGMIYFDLRPSSHAPTLELRVCD
Mb0441|M.bovis_AF2122/97 GLPFHFQRWAEFEGFVYDQKKTGIIDHMDEIRWDIRPSPHLGTLEVRICD
Rv0433|M.tuberculosis_H37Rv GLPFHFQRWAEFEGFVYDQKKTGIIDHMDEIRWDIRPSPHLGTLEVRICD
MAV_4721|M.avium_104 GLPFQFQTWAEFEGFVYDQKKTGIIDHVDEVRWDIRPSPHLGTLEMRICD
TH_0948|M.thermoresistible__bu GLPFQFQTWAEFERFVADQKKTGIIDHMNEIRWDIRPSPHLGTIEVRVFD
* . .: .:: * : . . : :*:*** *:*:*: *
MSMEG_5590|M.smegmatis_MC2_155 VPATVAETVLFATLVRAAVMTATEAEKNGEPVVPLTDYVLKAAYWKSARD
MAB_2558|M.abscessus_ATCC_1997 VPATVAETVLLATLVRAAVMSALEAQKRGDPAPRLEDCVLRAAHWRSARD
Mflv_1816|M.gilvum_PYR-GCK VPATVAETVLFAAIVRGCVMTALDDEARGEPVRPLAPYVLKAAYWMAARS
Mvan_4929|M.vanbaalenii_PYR-1 VPATVDETVLFAALVRGCVMTALEDERHGDPVLPLAPYALKAAYWKAARN
MMAR_5020|M.marinum_M SCPSVDTIVLVAGLYRAAVAREAEAIRAGTPAPAFPSTVGSAALWRAARS
MUL_4094|M.ulcerans_Agy99 SCPSVDTIVLVAGLYRAAVAREAEAFRAGTPAPAFPSTVGSAALWRAARS
Mb0441|M.bovis_AF2122/97 GVSNLRELGALVALTHCLIVDLDRRLDAGETLPTMPPWHVQENKWRAARY
Rv0433|M.tuberculosis_H37Rv GVSNLRELGALVALTHCLIVDLDRRLDAGETLPTMPPWHVQENKWRAARY
MAV_4721|M.avium_104 GVSNLHELAALVALTHCLVVDLDRRLEADESLPTMPPWHHQENKWRAARY
TH_0948|M.thermoresistible__bu GISNLRELGALVALTHCLVVDLDRRLDAGEQLPTMPPWHVQENKWRAARY
..: .. : : : . : * :**
MSMEG_5590|M.smegmatis_MC2_155 GLDGRTIDLAES-HAVAPTVELLTNFVEHLRPALEQLGEYDTVRGELARV
MAB_2558|M.abscessus_ATCC_1997 GLDGMAVDLVGS-SSTAPARTLLSRLIDHLRPALESLGDYDMARTELARV
Mflv_1816|M.gilvum_PYR-GCK GIEGDGVDLSD--HGAAPMVELLDRLVERIGPALDASGDGELVSSGLARV
Mvan_4929|M.vanbaalenii_PYR-1 GVDGDGVDLEN--HVPAPVSDLLGYLTERTRPALEAAGDHELVTAGLARV
MMAR_5020|M.marinum_M GLEGDLVDLTGPVPASRPAGDVVNDLVSSLRPQLEASGDWEMIGELTRQT
MUL_4094|M.ulcerans_Agy99 GLEGDLVDLTGPVPASRPAGDVVNDLVSSLRPQLEASGDWEMIGELTRQT
Mb0441|M.bovis_AF2122/97 GLDAVIILDADS--NERLVTDDLADVLTRLEPVAKSLNCADELAAVSDIY
Rv0433|M.tuberculosis_H37Rv GLDAVIILDADS--NERLVTDDLADVLTRLEPVAKSLNCADELAAVSDIY
MAV_4721|M.avium_104 GLDAVIILDADS--NERLVTEDLDDVLNRLEPVARKLQCADELAAVADIP
TH_0948|M.thermoresistible__bu GLDAEIILDADS--NERLVTDDLDDILNRLEPVAVSLNCADELAEVEHIY
*::. : : . * :
MSMEG_5590|M.smegmatis_MC2_155 IETGNGAMRQRRAFERRREAADVIDELAAATIAE----------------
MAB_2558|M.abscessus_ATCC_1997 VAQGNGAMRQRRTWCTSGNVKDVISEIATATTDFN---------------
Mflv_1816|M.gilvum_PYR-GCK AEHGNGAARQRRAWRRRHDVADVLAESAAATVEFP---------------
Mvan_4929|M.vanbaalenii_PYR-1 TDLGNGATRQRRAWRRNHDVDDVLAEAAAATLEDG---------------
MMAR_5020|M.marinum_M LLSGTSSARQRRALRRRGRLTDVVDQLIAETAGRVKPTTAAVSHDGGLLA
MUL_4094|M.ulcerans_Agy99 LLSGTSSARQRRALRRRGRLTDVVDQLIAETAGRVKPTTAAVSHDGGLLA
Mb0441|M.bovis_AF2122/97 RDGASYQRQLRVAQQHDGDLRAVVDALVAELVI-----------------
Rv0433|M.tuberculosis_H37Rv RDGASYQRQLRVAQQHDGDLRAVVDALVAELVI-----------------
MAV_4721|M.avium_104 RHGASYQRQRRVAEEHDGDLRAVVDALVAELEI-----------------
TH_0948|M.thermoresistible__bu RTGASYQRQRRVAEEHDGDLRAVVDALIGELDLGEPEQQGAS--------
.. : * : *:
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M AYQPTGEAGKPADDMFASYDEAVDADGRARPNYKTALKTIEGLGVDVLRA
MUL_4094|M.ulcerans_Agy99 PYQLTGEAGKPADGMFASYDEAVDADGRARPNYKTALKTIEGLGVDVLRA
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M RDRDIEQERRAGNVTFRAAGHSRVQVNPLDLVPRIVTADEWAQLSQGLAQ
MUL_4094|M.ulcerans_Agy99 RDRDIEQERSAGNVTFRAAGHSRVQVNPLDLVPRIVTADEWAQLSQGLAQ
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M RARALDAFLRDVYSEQAILADGVLSAQVLDRSPGFRSTGRLAGDGVRAHI
MUL_4094|M.ulcerans_Agy99 RARALDAFLRDVYSEQAILADGVLSAQVLDRSPGFRSTGRLAGDGVRAHI
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M SGIDLVCDRAGNWMVLEDNLRIPSGVAYAIVNRRLLGKYLPELPPPGGVA
MUL_4094|M.ulcerans_Agy99 SGIDLVCDRAGNWMVLEDNLRIPSGVAYAIVNRRLLGKYLPELPPPGGVA
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M DLDRVAHLLLETLRAAAPPHTPDQPTVTLLSAGRDDPGWFEHGFLAEEMG
MUL_4094|M.ulcerans_Agy99 DLDRVAHLLLETLRAASPPHTPDQPTVTLLSAGRDDPGWFEHGFLAEEMG
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M VALVAPSDLSVRDRRVFRHGGSGGSPVDVIYSRMYEDMLLSSTGHDGAPL
MUL_4094|M.ulcerans_Agy99 VALVAPSDLSVRDRRVFRHGGSGGSPVDVIYSRMYEDMLLSSTGHDGAPL
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M RSGLLEALDAGNLTIVNALGNGVAEDKAVYPFVPAMIEYYLGEKPALAQV
MUL_4094|M.ulcerans_Agy99 RSGLLEALDAGNLTIVNALGNGVAEDKAVYPFVPAMIEYYLGEKPALAQV
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M PTWVCAEREQRDYVLDHLGELVVKPTDGLSGSGVLIGPEATDAAIEARRR
MUL_4094|M.ulcerans_Agy99 PTCVCAEREQRDYVLDHLGELVVKPTDGLSGSGVLIGPEATDAAIEARRR
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 --------------------------------------------------
MAB_2558|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_1816|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5020|M.marinum_M ELLIQPERFVAQQLVALSTHPTFDDDGLYPHHVDLRAFVHLRQAPRGPVT
MUL_4094|M.ulcerans_Agy99 ELLIQPERFVAQQLVALSTHPTFADDGLYPHHVDLRAFVHLRQAPRGPVT
Mb0441|M.bovis_AF2122/97 --------------------------------------------------
Rv0433|M.tuberculosis_H37Rv --------------------------------------------------
MAV_4721|M.avium_104 --------------------------------------------------
TH_0948|M.thermoresistible__bu --------------------------------------------------
MSMEG_5590|M.smegmatis_MC2_155 ------------------------------------
MAB_2558|M.abscessus_ATCC_1997 ------------------------------------
Mflv_1816|M.gilvum_PYR-GCK ------------------------------------
Mvan_4929|M.vanbaalenii_PYR-1 ------------------------------------
MMAR_5020|M.marinum_M AKVLPAALTRVASRGSRIANSSSGAGSKDTWIFTDG
MUL_4094|M.ulcerans_Agy99 AKVLPAALTRVASRGSRIANSSSGAGSKDTWIFTDG
Mb0441|M.bovis_AF2122/97 ------------------------------------
Rv0433|M.tuberculosis_H37Rv ------------------------------------
MAV_4721|M.avium_104 ------------------------------------
TH_0948|M.thermoresistible__bu ------------------------------------