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RRIHVIGIGAGDPDYVTAQAVAALNDTEVFFAMDKGQSKDELVALRRLICERFITEARYRFVELADPKRA EDGDYRQIVADWHAERARIWARAIESELGPDGVGAFLAWGDPSLYDSTLRILEMVGEHVEFDYDVIPGVT AIQALTARHRIPLNDVGEPVLITTGRRLREHGLTGSAVVMLDADCSFRTCDPRTRIWWGAYLGTPDELLV AGTVGDVAEDIVRMRAEARTRHGWIMDTYLLRSAD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5548 | cobF | - | 100% (246) | precorrin 6A synthase |
| M. smegmatis MC2 155 | MSMEG_3873 | - | 9e-09 | 29.69% (192) | cobalamin biosynthesis protein cobIJ |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2092 | cobI | 1e-05 | 25.97% (154) | bifunctional S-adenosyl-L-methionine-precorrin-2 methyl |
| M. gilvum PYR-GCK | Mflv_0361 | - | 2e-44 | 43.70% (254) | precorrin 6A synthase |
| M. tuberculosis H37Rv | Rv2066 | cobI | 1e-05 | 25.97% (154) | bifunctional S-adenosyl-L-methionine-precorrin-2 methyl |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_1037 | - | 2e-91 | 66.80% (247) | precorrin 6A synthase |
| M. marinum M | MMAR_4563 | - | 1e-109 | 76.54% (243) | hypothetical protein MMAR_4563 |
| M. avium 104 | MAV_1065 | cobF | 1e-109 | 75.41% (244) | precorrin 6A synthase |
| M. thermoresistible (build 8) | TH_2324 | cobF | 1e-118 | 83.47% (242) | precorrin-6A synthase (deacetylating) |
| M. ulcerans Agy99 | MUL_4422 | - | 1e-109 | 76.13% (243) | precorrin 6A synthase |
| M. vanbaalenii PYR-1 | Mvan_0376 | - | 4e-43 | 41.18% (255) | precorrin 6A synthase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_5548|M.smegmatis_MC2_155 ----------------------MRRIHVIGIGAGDPDYVTAQAVAALNDT
TH_2324|M.thermoresistible__bu ---------------------VPRHIHVIGIGAGDPDYVTAQAVAALNDT
MMAR_4563|M.marinum_M --------------------MVSRHIHVIGIGAGDPDYVTVQAINALNDT
MUL_4422|M.ulcerans_Agy99 --------------------MVSRHIHVIGIGAGDPDYVTVQAINALNDT
MAV_1065|M.avium_104 ---------------------MGRHIHVIGIGAGDPDYLTVQAIEALNDT
MAB_1037|M.abscessus_ATCC_1997 ----------------------MRRIRVIGIGAGHPEYLTVQAIAALNEV
Mflv_0361|M.gilvum_PYR-GCK MRLRRGCSATGERARDNRCMAAAVTIRILGVGMG-PQHVTPEVADALNGV
Mvan_0376|M.vanbaalenii_PYR-1 -------------------MVAAVKVRILGVGMG-PQHVTPEVADALRTV
Mb2092|M.bovis_AF2122/97 -------------------MSARGTLWGVGLGPGDPELVTVKAARVIGEA
Rv2066|M.tuberculosis_H37Rv -------------------MSARGTLWGVGLGPGDPELVTVKAARVIGEA
: :*:* * *: :* :. .: .
MSMEG_5548|M.smegmatis_MC2_155 EVFFAMDKGQSKDELVALRRLICERFITEARYRFVELADPKRAE-----D
TH_2324|M.thermoresistible__bu DVFFAMDKGEAKDDLVALRRQICERFIERPGYRFVELPDPPRAR-----D
MMAR_4563|M.marinum_M QVFFAMDKGEAKSELVELRRAICARFIREPRYRFVELPDPKRAV-----D
MUL_4422|M.ulcerans_Agy99 QVFFAMDKGEAKSELVELRRAICARFIREPRYRFVELPDPKRAV-----D
MAV_1065|M.avium_104 QVFFAMDKGEQKSDLVALRREICSRFIRQPGYRFVELPDPKRSD-----R
MAB_1037|M.abscessus_ATCC_1997 DVFFVADKGDIKDDLVELRRHICERYITDPDYRFVELPDPVRG------K
Mflv_0361|M.gilvum_PYR-GCK DYVLAADKGE-DDALLALRREIVR---AHGGAEVLGLRDPARDRSDGLST
Mvan_0376|M.vanbaalenii_PYR-1 DYVLAAEKAD-DDRLLALRRAIVR---AHGGAELVALPDPPRDRSERLSA
Mb2092|M.bovis_AF2122/97 DVVAYHSAPHGHSIARGIAEPYLRPGQLEEHLVYPVTTEATNHP------
Rv2066|M.tuberculosis_H37Rv DVVAYHSAPHGHSIARGIAEPYLRPGQLEEHLVYPVTTEATNHP------
: . . . .. : . :. .
MSMEG_5548|M.smegmatis_MC2_155 GDYRQIVADWHAERARIWARAIESELGPDGVGAFLAWGDPSLYDSTLRIL
TH_2324|M.thermoresistible__bu GDYRQAVSDWHAARARIWADAIATELGPDGVGAFLAWGDPSLYDSTLRIL
MMAR_4563|M.marinum_M AHYEDAVSQWHAQRARIWARAISTELGPGGVGAFLAWGDPSLYDSTLRIL
MUL_4422|M.ulcerans_Agy99 AHYEDAVSQWHAQRARIWARAISTELGPGGVGAFLAWGDPSLYDSTLRIL
MAV_1065|M.avium_104 ADYRDAVAEWHAARAQIWADAIAAELGPHGVGGFLAWGDPSLYDSTLRIL
MAB_1037|M.abscessus_ATCC_1997 GEYRGAVTQWHAERAALWAKAIASDLPDGGTGAFLAWGDPSLYDSTLRIL
Mflv_0361|M.gilvum_PYR-GCK GAYESAVADWHDARAAQYADVLR---RRGGTAAFLVWGDPSLYDSTIRVV
Mvan_0376|M.vanbaalenii_PYR-1 GGYESAVSDWHDARAAQYAGVLK---ERGGTAAFLVWGDPSLYDSTIRVV
Mb2092|M.bovis_AF2122/97 GGYAGALEDFYADATERIATHLD----AGRNVALLAEGDPLFYSSYMHLH
Rv2066|M.tuberculosis_H37Rv GGYAGALEDFYADATERIATHLD----AGRNVALLAEGDPLFYSSYMHLH
. * : ::: : * : .:*. *** :*.* :::
MSMEG_5548|M.smegmatis_MC2_155 EM------------------------------------------------
TH_2324|M.thermoresistible__bu DR------------------------------------------------
MMAR_4563|M.marinum_M DA------------------------------------------------
MUL_4422|M.ulcerans_Agy99 DA------------------------------------------------
MAV_1065|M.avium_104 DT------------------------------------------------
MAB_1037|M.abscessus_ATCC_1997 DAI-----------------------------------------------
Mflv_0361|M.gilvum_PYR-GCK EKVR----------------------------------------------
Mvan_0376|M.vanbaalenii_PYR-1 EKVR----------------------------------------------
Mb2092|M.bovis_AF2122/97 TRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVLPGTLPVGELTR
Rv2066|M.tuberculosis_H37Rv TRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVLPGTLPVGELTR
MSMEG_5548|M.smegmatis_MC2_155 ---------------------------------------------VGEHV
TH_2324|M.thermoresistible__bu ---------------------------------------------VAEHV
MMAR_4563|M.marinum_M ---------------------------------------------VSAEV
MUL_4422|M.ulcerans_Agy99 ---------------------------------------------VSAEV
MAV_1065|M.avium_104 ---------------------------------------------VAGHV
MAB_1037|M.abscessus_ATCC_1997 --------------------------------------------LVDDPA
Mflv_0361|M.gilvum_PYR-GCK -------------------------------------------DLLGPDT
Mvan_0376|M.vanbaalenii_PYR-1 -------------------------------------------ALLEPHV
Mb2092|M.bovis_AF2122/97 RLADADAAVVVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAA
Rv2066|M.tuberculosis_H37Rv RLADADAAVVVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAA
: .
MSMEG_5548|M.smegmatis_MC2_155 EFDYDVIPGVTAIQALTARHRIPLNDVGEPVLITTG--------------
TH_2324|M.thermoresistible__bu EISYDVIPGITAIQALTARHRIPLNDVGEPVLITTG--------------
MMAR_4563|M.marinum_M DFSYDVIPGITAVQALTARHRIPLNDVGAPVVITTG--------------
MUL_4422|M.ulcerans_Agy99 DFSYDVIPGITAVQALTARHRIPLNDVGAPVVITTG--------------
MAV_1065|M.avium_104 EFTFDVIPGITAVQALTARHRIPLNEVGEPVLVTTG--------------
MAB_1037|M.abscessus_ATCC_1997 AFEYDVLPGITAISALTARHRIVLNGIGEPVLITTG--------------
Mflv_0361|M.gilvum_PYR-GCK DVDFDVLPGISAPQLLAARHRIVLHEVGRPVHITTG--------------
Mvan_0376|M.vanbaalenii_PYR-1 ALDFDVLPGISAPQLLAARHRIVLHEVGKPVHVTTG--------------
Mb2092|M.bovis_AF2122/97 DVDETSVPYFSLAMLPGGRRRALLTGTVAVVGLGPGDSDWMTPQSRRELA
Rv2066|M.tuberculosis_H37Rv DVDETSVPYFSLAMLPGGRRRALLTGTVAVVGLGPGDSDWMTPQSRRELA
. :* .: .*:* * * : .*
MSMEG_5548|M.smegmatis_MC2_155 --------------------------------RRLR-EHGLTG--SAVVM
TH_2324|M.thermoresistible__bu --------------------------------RRLR-EHGLTG--AAVVM
MMAR_4563|M.marinum_M --------------------------------RQLR-ARQVAG--TAVVM
MUL_4422|M.ulcerans_Agy99 --------------------------------RQLR-TRQVAG--TAVVM
MAV_1065|M.avium_104 --------------------------------RQLR-AHGISG--SAVVM
MAB_1037|M.abscessus_ATCC_1997 --------------------------------RRLLDEWPRAG--TVVVM
Mflv_0361|M.gilvum_PYR-GCK --------------------------------RRLQ-EAVAAGLDNILAM
Mvan_0376|M.vanbaalenii_PYR-1 --------------------------------RRLQ-EAVAAGMDNIVAM
Mb2092|M.bovis_AF2122/97 AATDLIGYRGYLDRVEVRDGQRRHPSDNTDEPARARLACSLADQGRAVAV
Rv2066|M.tuberculosis_H37Rv AATDLIGYRGYLDRVEVRDGQRRHPSDNTDEPARARLACSLADQGRAVAV
: :. :.:
MSMEG_5548|M.smegmatis_MC2_155 LDA---------DCSFRTCDPRTRIWWGAYLGTPDELLVAGTVGDVA---
TH_2324|M.thermoresistible__bu LDG---------ECSFLTCPPQTRIWWGAYLGTPDELLLAGTVGAVG---
MMAR_4563|M.marinum_M LDA---------DCSFRACPPQTRIWWGAYLGTEDELLVAGTVGEVG---
MUL_4422|M.ulcerans_Agy99 LDA---------DCSFRACPTQTRIWWGAYLGTEDELLVAGTVGEVG---
MAV_1065|M.avium_104 LDA---------DCSFQRCAPETRIWWGAYLGTADELLVAGTVGEVG---
MAB_1037|M.abscessus_ATCC_1997 LDG---------DCAFRQLDPSTQIWWGAYLGTEHELLVAGTVGEVG---
Mflv_0361|M.gilvum_PYR-GCK LNPAP------DRLDFTGLDDWT-IWWGANLGAAGERLVAGRVGDVL---
Mvan_0376|M.vanbaalenii_PYR-1 LNPPP------ERLDFTGLDDWT-IWWGANLGAPGERLVTGRLRDVL---
Mb2092|M.bovis_AF2122/97 VSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAVASRVGAPLGHD
Rv2066|M.tuberculosis_H37Rv VSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAVASRVGAPLGHD
:. : . : . . ::. :
MSMEG_5548|M.smegmatis_MC2_155 ------------EDIVRMRAEARTRHGWIMDTYLLRSAD-----------
TH_2324|M.thermoresistible__bu ------------KRIETVRAEARARHGWIMDTYLLRP-------------
MMAR_4563|M.marinum_M ------------ERIAALRAEARARHGWIMDTYLIRTY------------
MUL_4422|M.ulcerans_Agy99 ------------ERIAALRAEARARHGWIMDTYLIRTY------------
MAV_1065|M.avium_104 ------------PRIAALRAQARARHGWIMDTYLLRPAH-----------
MAB_1037|M.abscessus_ATCC_1997 ------------DRIAQVRTEARADHGWIMDTYLLRSVGA----------
Mflv_0361|M.gilvum_PYR-GCK ------------PAIADARAAAHADDGWVMDAFLIRRTR-----------
Mvan_0376|M.vanbaalenii_PYR-1 ------------PAVADARAAADAYDGWVMDVFLIRRDR-----------
Mb2092|M.bovis_AF2122/97 YAVISLSDRLKPWDVIAARLTAAAAADLVLAIYNPASVTRTWQVGAMREL
Rv2066|M.tuberculosis_H37Rv YAVISLSDRLKPWDVIAARLTAAAAADLVLAIYNPASVTRTWQVGAMREL
: * * : . :: :
MSMEG_5548|M.smegmatis_MC2_155 --------------------------------------------------
TH_2324|M.thermoresistible__bu --------------------------------------------------
MMAR_4563|M.marinum_M --------------------------------------------------
MUL_4422|M.ulcerans_Agy99 --------------------------------------------------
MAV_1065|M.avium_104 --------------------------------------------------
MAB_1037|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_0361|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_0376|M.vanbaalenii_PYR-1 --------------------------------------------------
Mb2092|M.bovis_AF2122/97 LLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVG
Rv2066|M.tuberculosis_H37Rv LLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVG
MSMEG_5548|M.smegmatis_MC2_155 -------------------------------------
TH_2324|M.thermoresistible__bu -------------------------------------
MMAR_4563|M.marinum_M -------------------------------------
MUL_4422|M.ulcerans_Agy99 -------------------------------------
MAV_1065|M.avium_104 -------------------------------------
MAB_1037|M.abscessus_ATCC_1997 -------------------------------------
Mflv_0361|M.gilvum_PYR-GCK -------------------------------------
Mvan_0376|M.vanbaalenii_PYR-1 -------------------------------------
Mb2092|M.bovis_AF2122/97 SSQTRWYSVDSQDRVFTPRRYPEAGRATATKSSRHSD
Rv2066|M.tuberculosis_H37Rv SSQTRWYSVDSQDRVFTPRRYPEAGRATATKSSRHSD