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VWVESPGEHIAGFVVEDVAGRGGWAVVYRARDAQGRLVALKVLNESHREPAHLERLRREFEFACRAEHPN VVAMYAAGDGWLAMEFIDGRPAISLAVRTDQLTALRQIAGALDNIHRLGIVHSDVKPMNILVDKPFSRAV LIDFGVAHSLAEDVAARLAADATGRLTLDPARRITHQSAAPRPQVQASLPYSAPELITGRALSAATDQYA LACTAVEILTGLPPFTATTAVKMVDQQLNSPPPRISGRAAWIPHAVDAILAKALSKDPERRHESCTEFVD ELAAALG
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5437 | - | - | 100% (287) | serine/threonine-protein kinase PknB |
| M. smegmatis MC2 155 | MSMEG_1190 | - | 2e-35 | 34.80% (296) | serine/threonine-protein kinase PknD |
| M. smegmatis MC2 155 | MSMEG_3677 | - | 4e-33 | 33.22% (298) | serine/threonine protein kinase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2115 | pknJ | 7e-38 | 35.45% (299) | transmembrane serine/threonine-protein kinase J |
| M. gilvum PYR-GCK | Mflv_3427 | - | 1e-31 | 31.54% (298) | protein kinase |
| M. tuberculosis H37Rv | Rv2088 | pknJ | 7e-38 | 35.45% (299) | transmembrane serine/threonine-protein kinase J |
| M. leprae Br4923 | MLBr_00016 | pknB | 2e-29 | 31.10% (283) | putative serine/threonine protein kinase |
| M. abscessus ATCC 19977 | MAB_1133c | - | 4e-61 | 47.75% (289) | putative serine/threonine protein kinase |
| M. marinum M | MMAR_2941 | - | 9e-40 | 35.79% (299) | membrane-anchored serine/threonine-protein kinase |
| M. avium 104 | MAV_3463 | - | 6e-36 | 35.29% (289) | protein kinase/peptidyl-prolyl cis-trans isomerase, |
| M. thermoresistible (build 8) | TH_4002 | - | 1e-74 | 51.41% (284) | PUTATIVE putative probable serine/threonine-protein kinase PknB |
| M. ulcerans Agy99 | MUL_2200 | - | 8e-40 | 35.79% (299) | membrane-anchored serine/threonine-protein kinase |
| M. vanbaalenii PYR-1 | Mvan_4693 | - | 2e-31 | 32.53% (292) | protein kinase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_5437|M.smegmatis_MC2_155 ----MWVESPGEHIAGFVVEDVAGRGGWAVVYRAR-DAQGRLVALKVLNE
MAB_1133c|M.abscessus_ATCC_199 ------MPVPGEEFHGYVVGSLAGRGGTAQVYLAHRVDDGFEVALKVLEL
TH_4002|M.thermoresistible__bu ------VLRRGDRFENYVIDDELGRGGSAIVYRAREADGGRVVALKVLDP
Mb2115|M.bovis_AF2122/97 ---MAHELSAGSVFAGYRIERMLGAGGMGTVYLARNPDLPRSEALKVLAA
Rv2088|M.tuberculosis_H37Rv ---MAHELSAGSVFAGYRIERMLGAGGMGTVYLARNPDLPRSEALKVLAA
MMAR_2941|M.marinum_M -----MPLNEGDIFAGYVIQRLLGTGGMGEVYLAQHPRLPRLDALKILSL
MUL_2200|M.ulcerans_Agy99 -----MPLNEGDIFAGYVIQRLLGTGGMGEVYLAQHPRLPRLDALKILSL
Mflv_3427|M.gilvum_PYR-GCK -----MPLAAGETFAGYTIVRLLGSGGMGEVYLAQHPRLPRRDALKVLPA
MAV_3463|M.avium_104 ------MTLDPDSFGHYRILELLGRGGMGRVYRAYDATTDRVVALKVLPP
Mvan_4693|M.vanbaalenii_PYR-1 MTDPQRGSRVGSQVGPYRLRRLLGKGGMGEVYEAEDTVKDRVVALKLLPE
MLBr_00016|M.leprae_Br4923 ------MTTPPHLSDRYELGDILGFGGMSEVHLARDIRLHRDVAVKVLRA
: : * ** . *: * *:*:*
MSMEG_5437|M.smegmatis_MC2_155 SHR-EPAHLERLRREFEFACRAEHPNVVAMYAAGD--------GWLAMEF
MAB_1133c|M.abscessus_ATCC_199 RHR-NPENIDRLHREFDLAQRFSHPRIVTVFERGQ--------DWLTMEA
TH_4002|M.thermoresistible__bu HHR-SAADLERLRREYEFAARLDHPRVVTMYGAGP--------GWLAMEL
Mb2115|M.bovis_AF2122/97 ELSRDLDFRARFVREADVAAGLDHPNIVAVHQRGQFEG----RLWIAMQF
Rv2088|M.tuberculosis_H37Rv ELSRDLDFRARFVREADVAAGLDHPNIVAVHQRGQFEG----RLWIAMQF
MMAR_2941|M.marinum_M DATDDDEFRARFTREAELAATLWHPHIVGVHDRGEFDG----RLWISMDY
MUL_2200|M.ulcerans_Agy99 DATDDDEFRARFTREAELAATLWHPHIVGVHDRGEFDG----RLWISMDY
Mflv_3427|M.gilvum_PYR-GCK AVSADVEYRKRFEREADIAATLWHPHIVGVHDRGEDAG----RIWISMDY
MAV_3463|M.avium_104 HLAEDQDFQQRFRREARIAAGLNDPHVVPIHGYGEIDG----QLYVDMRL
Mvan_4693|M.vanbaalenii_PYR-1 AVSHDPVFRKRLQREAHAAGRLQEPHVVPIHDYGEVDG----LLFVDMRL
MLBr_00016|M.leprae_Br4923 DLARDPSFYLRFRREAQNAAALNHPSIVAVYDTGEAETSAGPLPYIVMEY
. *: ** * .* :* :. * :: *
MSMEG_5437|M.smegmatis_MC2_155 IDGRPAISLAVRTD--------QLTALRQIAGALDNIHRLGIVHSDVKPM
MAB_1133c|M.abscessus_ATCC_199 LTGGSAKDLVGTNQS--WIIPLKLTLLQQIAGALDFIHDEQVVHCDVKPS
TH_4002|M.thermoresistible__bu LAGGPVSNLPRLAD--------RLTALADVAAALDHIHYRGVVHCDVKPA
Mb2115|M.bovis_AF2122/97 VDGGNAEDALRAAT---MTTARAVYVIGEVAKALDYAHQQGVIHRDIKPA
Rv2088|M.tuberculosis_H37Rv VDGGNAEDALRAAT---MTTARAVYVIGEVAKALDYAHQQGVIHRDIKPA
MMAR_2941|M.marinum_M VDGTDARHLVEQRYRSGMPLEDVIEIVTAVAEALDFAHERRLLHRDVKPA
MUL_2200|M.ulcerans_Agy99 VDGTDTRHLVEQRYRSGMPLEDVIEIVTAVAEALDFAHERRLLHRDVKPA
Mflv_3427|M.gilvum_PYR-GCK VEGADAAHLLAEDYRTGLPVAEVAQIVTAVADALDYAHDRHLLHRDVKPA
MAV_3463|M.avium_104 IEGRDLAHYITENGG-RLSPQRAVAVIEQVAAALDSAHRAGLIHRDVKPM
Mvan_4693|M.vanbaalenii_PYR-1 IDGTDLRKLLKEQG--PMTPARATAIVAQVASALDAAHQNGIMHRDVKPE
MLBr_00016|M.leprae_Br4923 VDGATLRDIVHTDGP--MPPQQAIEIVADACQALNFSHQNGIIHRDVKPA
: * : . **: * ::* *:**
MSMEG_5437|M.smegmatis_MC2_155 NILVDK----PFSRAVLIDFGVAHSLAEDVAARLAADATGRLTLDPARRI
MAB_1133c|M.abscessus_ATCC_199 NIMRTR----SGS-AVLTDFGIAQELA-----------------------
TH_4002|M.thermoresistible__bu NILSGED--FAGTGGVLTDFGVAHAVSEFIKP-----------------D
Mb2115|M.bovis_AF2122/97 NFLLSRAAGGDER-VLLSDFGIARALG-----------------------
Rv2088|M.tuberculosis_H37Rv NFLLSRAAGGDER-VLLSDFGIARALG-----------------------
MMAR_2941|M.marinum_M NILVTEPSESARRRVLLTDFGIAREID-----------------------
MUL_2200|M.ulcerans_Agy99 NILVTEPSESARRRVLLTDFGIAREID-----------------------
Mflv_3427|M.gilvum_PYR-GCK NILIARP-DSNTRRIMLADFGIARWDN-----------------------
MAV_3463|M.avium_104 NVLVTTAR----DFVYLIDFGLARAQA-----------------------
Mvan_4693|M.vanbaalenii_PYR-1 NILINRD-----DFAYLVDFGIANAVT-----------------------
MLBr_00016|M.leprae_Br4923 NIMISAT-----NAVKVMDFGIARAIA-----------------------
*.: : ***:*.
MSMEG_5437|M.smegmatis_MC2_155 THQSAAPRPQVQASLPYSAPELITGRALSAATDQYALACTAVEILTGLPP
MAB_1133c|M.abscessus_ATCC_199 -HADGPRRPVVQTSLPYAAPEVLRGQPVTAATDQYALACTAVELFDGKPP
TH_4002|M.thermoresistible__bu AHTVSRRRVPVEASLAYTAPELFGGRPPSAASDEYALACTAVELVTGAPP
Mb2115|M.bovis_AF2122/97 DTG-LTSTGSVLATLAYAAPEVLAGQGFDGRADLYSLGCALFRLLTGEAP
Rv2088|M.tuberculosis_H37Rv DTG-LTSTGSVLATLAYAAPEVLAGQGFDGRADLYSLGCALFRLLTGEAP
MMAR_2941|M.marinum_M DKHRLTEAQMAIGTVAYAAPEQLTGKPLDGRADQYALAATAFHLLAGVPP
MUL_2200|M.ulcerans_Agy99 DKHRLTEAQMAIGTVAYAAPEQLTGRPLDGRADQYALAATAFHLLAGVPP
Mflv_3427|M.gilvum_PYR-GCK DISGLTATNMTVGTVSYAAPEQLMGQDLDGRADQYALAATAFHLLTGSPP
MAV_3463|M.avium_104 -DTALTQTGATMGTVAYMAPERFT-GTTDHRADVYSLACVLHECLTGKRP
Mvan_4693|M.vanbaalenii_PYR-1 -DEALTELGTAVGTYAYMAPERFTNGEVTHRADVYALTCVLHECLTGAQP
MLBr_00016|M.leprae_Br4923 DSTSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEILTGEPP
. : * :** :* *:* .. . . * *
MSMEG_5437|M.smegmatis_MC2_155 FTATT--AVKMVDQQLNSPPPRISGRAAWIPHAVDAILAKALSKDPERRH
MAB_1133c|M.abscessus_ATCC_199 FTART--MLKLADMHLNAAPWPISYRHKYIPRAFDSIVAKAMSKDPLSRY
TH_4002|M.thermoresistible__bu FVATT--RMALIDQHLHRPPPALSRRFEWVPRAFDSVLARALAKLPDARY
Mb2115|M.bovis_AF2122/97 FAAGAGAAVAVVAGHLHQPPPTVSDRVPGLSAAMDAVIATAMAKDPMRRF
Rv2088|M.tuberculosis_H37Rv FAAGAGAAVAVVAGHLHQPPPTVSDRVPGLSAAMDAVIATAMAKDPMRRF
MMAR_2941|M.marinum_M FDHSN--RAVVVGQHLNMPPPRISKRHP-KLAHLDAAFAKALAKDPDDRY
MUL_2200|M.ulcerans_Agy99 FDHSN--RAVVVGQHLNMPPPRISKRHP-KLAHLDAAFAKALAKDPDDRY
Mflv_3427|M.gilvum_PYR-GCK FSHSN--PAVVISRHLNSAPATVAAHRP-ELAAVDPVLTRALAKNPADRY
MAV_3463|M.avium_104 FAGDS--LEEQLNAHLNTAPPRPSATAPEVPAAFDAVIARGMAKDPERRY
Mvan_4693|M.vanbaalenii_PYR-1 FQGDS--VSVVITAHLNDPAPRPSQSRPGIPAGLDDVISRGMAKRPEDRY
MLBr_00016|M.leprae_Br4923 FIGDS--PVSVAYQHVREDPIPPSQRHEGISVDLDAVVLKALAKNPENRY
* ::. . : .* . .::* * *.
MSMEG_5437|M.smegmatis_MC2_155 ESCTEFVDELAAALG-----------------------------------
MAB_1133c|M.abscessus_ATCC_199 PTCTEFITLLTHAMT-----------------------------------
TH_4002|M.thermoresistible__bu PTCTEFITLVRDALGTGPT-------------------------------
Mb2115|M.bovis_AF2122/97 TSAGEFAHAAAAALYGGATDGWVPPSPAPHVISQGAVPGS-----PWWQH
Rv2088|M.tuberculosis_H37Rv TSAGEFAHAAAAALYGGATDGWVPPSPAPHVISQGAVPGS-----PWWQH
MMAR_2941|M.marinum_M PRCIDFAKALAAGPGSIAEHQARRGATSTEPFDVNAEMSAEALEPARPSG
MUL_2200|M.ulcerans_Agy99 PRCIDFAKALAAGPGSIAEHQARRGATSTEPFDVNAEMSAEALEPARPSG
Mflv_3427|M.gilvum_PYR-GCK PRCADFAREFNARLDAGAPAHTSR---------------------IRPAV
MAV_3463|M.avium_104 QSVTELAEAARAALAPGAVEKPSAPTPQPRAARRVRAAVVGASALTLAVV
Mvan_4693|M.vanbaalenii_PYR-1 ASAGEMARAATAALTG---------AQQDQADSILQRSQAATMMAPLPPP
MLBr_00016|M.leprae_Br4923 QTAAEMRADLIRVHSGQPPEAPKVLTDADRSCLLSSGAGNFGVPRTDALS
::
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 PVGSVTALATPPGHGWPPGLPPLPRRPRRYRRGVAAVAAVMVVAAAAVTA
Rv2088|M.tuberculosis_H37Rv PVGSVTALATPPGHGWPPGLPPLPRRPRRYRRGVAAVAAVMVVAAAAVTA
MMAR_2941|M.marinum_M TAPSAQAQPHPSEAGVSTASLGAADQPGAGKARHTKRLERMTVDGTAVSV
MUL_2200|M.ulcerans_Agy99 TAPSAQAQPHPSEAGVSTASLGAADQPGAGKARHTKRLERMTVDGTAVSV
Mflv_3427|M.gilvum_PYR-GCK LIPVVLAVLLVAAVAVAAFEFLRADRADRADRADPPPMARTTTITTSPTT
MAV_3463|M.avium_104 AAVVVAMVTHGHGSHGATPKTPGSPAPGRTAPPLPAFVAPPDLGVNCQYR
Mvan_4693|M.vanbaalenii_PYR-1 PPPPPPSTPGGPLPYGGSPVPPPPPPPGQPTQASGFHTPPPPGGPPPGWP
MLBr_00016|M.leprae_Br4923 RQSLDETESDGSIGRWVAVVAVLAVLTIAIVAAFNTFGGNTRDVQVPDVR
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 VTMTSHQPRTATPPSAAALSPTSSSTTPPQPPIVTRSRLPGLLPPLDDVK
Rv2088|M.tuberculosis_H37Rv VTMTSHQPRTATPPSAAALSPTSSSTTPPQPPIVTRSRLPGLLPPLDDVK
MMAR_2941|M.marinum_M QKRPAGPEQDEEVWLFCVQRYESTGEPHTVIPVELRGTSITGQLANGDAV
MUL_2200|M.ulcerans_Agy99 QKRPAGPEQDEEVWLFCVQRYESTGEPHTVIPVELRGTSITGQLANGDAV
Mflv_3427|M.gilvum_PYR-GCK STTTATP------------------------PATPASVVIGANCAPLDAT
MAV_3463|M.avium_104 AVPDPSSRPVSPPPSGRVPTTPGQIGAVIATN------------LGDIGI
Mvan_4693|M.vanbaalenii_PYR-1 PQPPQSSGGGGGSKGPMIALLAGAAVIILVLAGLGIWLVVKPDSTSDPVA
MLBr_00016|M.leprae_Br4923 GQVSADAISALQNRGFKTRTLQKPDSTIPPDHVISTEPGANASVGAGDEI
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 NFVGIQNLVAHEPMLQPQTPNGSIN-------------------------
Rv2088|M.tuberculosis_H37Rv NFVGIQNLVAHEPMLQPQTPNGSIN-------------------------
MMAR_2941|M.marinum_M GVSGIWDGSTLFADAVVNYTAANRGRRRSSALKVEGGLKEGEASAPARPP
MUL_2200|M.ulcerans_Agy99 GVSGIWDGSTLFADAVVNYTAANRGRRRSSALKVEGGLKEGEASAPARPP
Mflv_3427|M.gilvum_PYR-GCK ATTG--DGTTAYCAAVP----GSAG-------------------------
MAV_3463|M.avium_104 SLANSESPCAVNSFISLARQRFFDN-------------------------
Mvan_4693|M.vanbaalenii_PYR-1 SRTSTSAAPTTTSRGSTTRTRTSTS-------------------------
MLBr_00016|M.leprae_Br4923 TINVSTGPEQREVPDVSSLNYTDAVK------------------------
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 ----------------------PAECWPAVGGGVPSAYDLGTVIGFYGLT
Rv2088|M.tuberculosis_H37Rv ----------------------PAECWPAVGGGVPSAYDLGTVIGFYGLT
MMAR_2941|M.marinum_M GSKSRKGRAFAIVAALLAIAAIAVAAVLTHGFGLWSTSEPGPVVKAQQAT
MUL_2200|M.ulcerans_Agy99 GSKSRKGRAFAIVAALLALAAIAVAAVLTHGFGLWSTSEPGPVVKAQQAT
Mflv_3427|M.gilvum_PYR-GCK --------------------------------TIWSLT---------QGT
MAV_3463|M.avium_104 ----------------------TQCARLVDSPDGGSLLCGGPDVDGSG--
Mvan_4693|M.vanbaalenii_PYR-1 ----------------------TPPPTESYDQQLMALLSGGHDSSNCEPV
MLBr_00016|M.leprae_Br4923 -------------------------KLTSSGFKSFKQANSPSTPELLGKV
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 IDEPPTGTAPNQVGQLIVAFRDAATAQRHLADLASIWRRCGGRTVTLFRS
Rv2088|M.tuberculosis_H37Rv IDEPPTGTAPNQVGQLIVAFRDAATAQRHLADLASIWRRCGGRTVTLFRS
MMAR_2941|M.marinum_M VFSP--GGTPDHPGQAGLAIDGDPNSAWPTDTYVDATPFPAFKQGVGLIL
MUL_2200|M.ulcerans_Agy99 VFSP--GGTPDHPGQAGLAIDGDPNSAWPTDTYVDATPFPAFKQGVGLIL
Mflv_3427|M.gilvum_PYR-GCK LPAP--TSTP------------------PPDP------------------
MAV_3463|M.avium_104 --GPGYEFADEYPANQYRPDDPALRATLLYP-RGTVVMATEGPNTNGSQF
Mvan_4693|M.vanbaalenii_PYR-1 DPPAPTILATVDCTQALTPGGPAFTRYSLFEDRGALDAAFEEAIRVNSEL
MLBr_00016|M.leprae_Br4923 IGTNPSANQTSAITNVITIIVGSGPETKQIPDVTGQIVEIAQKNLNVYGF
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 EWRRPVELSTSVPEVVDGITTMVLTAQGPVLRVREDHAIAAKNN------
Rv2088|M.tuberculosis_H37Rv EWRRPVELSTSVPEVVDGITTMVLTAQGPVLRVREDHAIAAKNN------
MMAR_2941|M.marinum_M RLAKPTALSEVTIDVPSTGTEVQIRAAGSATPASLSDTTALTPNVALHPG
MUL_2200|M.ulcerans_Agy99 RLAKPTALSEVTIDVPSTGTEVQIRAAGSATPASLSDTTALTPNVALHPG
Mflv_3427|M.gilvum_PYR-GCK ------------------------------TEQPLSDAEAFP--------
MAV_3463|M.avium_104 ALIFHDSEMDPQ--------STVLGTIDP---AGLATLDKIARA------
Mvan_4693|M.vanbaalenii_PYR-1 VQCPGSGVDSPTTWHYTETPDQVVGQIACGTYDGNPDLVWTKDD------
MLBr_00016|M.leprae_Br4923 TKFSQASVDSPRPTGEVIGTNPPKDATVPVDSVIELQVSKGNQFVMPDLS
MSMEG_5437|M.smegmatis_MC2_155 --------------------------------------------------
MAB_1133c|M.abscessus_ATCC_199 --------------------------------------------------
TH_4002|M.thermoresistible__bu --------------------------------------------------
Mb2115|M.bovis_AF2122/97 --------------VLVDVDIMTPDTSRGQQAVIGITNYILAKIPG----
Rv2088|M.tuberculosis_H37Rv --------------VLVDVDIMTPDTSRGQQAVIGITNYILAKIPG----
MMAR_2941|M.marinum_M HNRIRVDSTTKTANVLVWISKLGTTKGESRTAISDITLRAR---------
MUL_2200|M.ulcerans_Agy99 HNRIRVDSTTKTANVLVWISKLGTTKGESRNAISDITLRAR---------
Mflv_3427|M.gilvum_PYR-GCK --------------VLTCMEQTGQTRRECRRAIRESNGQPPLP-------
MAV_3463|M.avium_104 --------------GIAGNRPSGPPANPVTITSVRIG-------------
Mvan_4693|M.vanbaalenii_PYR-1 --------------DLLLADAQGPNLEELHNWWLEFG-------------
MLBr_00016|M.leprae_Br4923 GMFWADAEPRLRALGWTGVLDKGPDVDAGGSQHNRVAYQNPPAGAGVNRD
MSMEG_5437|M.smegmatis_MC2_155 ---------
MAB_1133c|M.abscessus_ATCC_199 ---------
TH_4002|M.thermoresistible__bu ---------
Mb2115|M.bovis_AF2122/97 ---------
Rv2088|M.tuberculosis_H37Rv ---------
MMAR_2941|M.marinum_M ---------
MUL_2200|M.ulcerans_Agy99 ---------
Mflv_3427|M.gilvum_PYR-GCK ---------
MAV_3463|M.avium_104 ---------
Mvan_4693|M.vanbaalenii_PYR-1 ---------
MLBr_00016|M.leprae_Br4923 GIITLKFGQ