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VNAASPRTTTNGFVNTATDPFSAVVVATPDLAGRLLGRRHSWPHYQASASKAVNTCEVVFGWGLGHELLD GFSGVGWDHGYGDLRFVTDPDTLRPMGWQDGVGIAFADVVTGDGTPFFASPRNILRKQIERAHSAGYQPL VTSELEFFALQHTSETLAQQGFTGLKPIRETLQPELACAISTDETIMAELFGALQRTGIEVETAKTEYCP GQYEVTLVPTDPMRMADQHALYKMATRQIFRRHGMSATFMAKVDDHWAGSSSHLHISLTDENGSNLFATD ENLLRHFGAGLQAFARDFFLLWAPYPNSYKRFRDGTFAPTALTWGDDNRTVALRVTGHGSARHLENRIPG ADINPYLGYAALLAAGLEGIERGLLPADEVGQRNAYTEKIGRPLPESLGEALDAFYDSPVTRQTLGEAAV EHITTFLSSELRASQLAVTDWDRRRLFDL*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_1116 | - | - | 100% (450) | gamma-glutamylisopropylamide synthetase |
| M. smegmatis MC2 155 | MSMEG_2595 | - | 5e-73 | 37.92% (451) | gamma-glutamylisopropylamide synthetase |
| M. smegmatis MC2 155 | MSMEG_3828 | - | 7e-62 | 33.94% (442) | glutamine synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2885c | glnA4 | 5e-77 | 39.09% (440) | glutamine synthetase |
| M. gilvum PYR-GCK | Mflv_4065 | - | 1e-70 | 36.57% (443) | glutamate--ammonia ligase |
| M. tuberculosis H37Rv | Rv2860c | glnA4 | 5e-77 | 39.09% (440) | glutamine synthetase |
| M. leprae Br4923 | MLBr_01631 | glnA2 | 4e-36 | 30.77% (416) | glutamine synthase class II |
| M. abscessus ATCC 19977 | MAB_1920 | - | 3e-39 | 31.06% (425) | glutamine synthetase |
| M. marinum M | MMAR_3294 | glnA2 | 1e-38 | 32.89% (377) | glutamine synthetase GlnA2 |
| M. avium 104 | MAV_3718 | glnA | 1e-76 | 38.90% (455) | glutamine synthetase catalytic domain |
| M. thermoresistible (build 8) | TH_3396 | - | 2e-39 | 30.47% (407) | PUTATIVE glutamine synthetase, type I |
| M. ulcerans Agy99 | MUL_1335 | glnA2 | 9e-39 | 32.89% (377) | glutamine synthetase GlnA2 |
| M. vanbaalenii PYR-1 | Mvan_1603 | - | 2e-70 | 37.44% (454) | glutamate--ammonia ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_3294|M.marinum_M -----------------MDRQKEFVLRTLEERD---IRFVRLWFTDVLG-
MUL_1335|M.ulcerans_Agy99 -----------------MDRQKEFVLRTLEERD---IRFVRLWFTDVLG-
MLBr_01631|M.leprae_Br4923 -----------------MDRQKEFVLRTLEERD---IRFVRLWFTDVLG-
TH_3396|M.thermoresistible__bu -----------------MDRQKEFVLRSLEERD---IRFVRLWFTDVLG-
MAB_1920|M.abscessus_ATCC_1997 -----------------MDRQKEFVLRTLEERD---IRFVRLWFTDVLG-
Mb2885c|M.bovis_AF2122/97 ----------MTGPGSPPLAWTELERLVAAGD----VDTVIVAFTDMQGR
Rv2860c|M.tuberculosis_H37Rv ----------MTGPGSPPLAWTELERLVAAGD----VDTVIVAFTDMQGR
MAV_3718|M.avium_104 ---MASEDSALTGSDTAMLSLAALDRLVAAGAPETRVDTVIVAFPDMQGR
Mflv_4065|M.gilvum_PYR-GCK -MTDRTKGRLELMTTRGMLTLEDLEKQVADGD----IDTVIVAFCDMQGR
Mvan_1603|M.vanbaalenii_PYR-1 MTNASIRPGRIDATNGQVESAPDAVRAMFERHG---IRTVQFGMADIDGQ
MSMEG_1116|M.smegmatis_MC2_155 ----------MVNAASPRTTTNGFVNTATDPFS-----AVVVATPDLAGR
* . *: *
MMAR_3294|M.marinum_M ---------FLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSES----D
MUL_1335|M.ulcerans_Agy99 ---------FLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSES----D
MLBr_01631|M.leprae_Br4923 ---------YLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSES----D
TH_3396|M.thermoresistible__bu ---------FLKSVAIAPAELEGAFEEGIGFDGSAIEGFARVSEA----D
MAB_1920|M.abscessus_ATCC_1997 ---------YLKSVSIAPAELEGAFEEGIGFDGSSIEGFARVSES----D
Mb2885c|M.bovis_AF2122/97 LAGKRISGRHFVDDIATRGVECCSYLLAVDVDLNTVPGYAMASWDTGYGD
Rv2860c|M.tuberculosis_H37Rv LAGKRISGRHFVDDIATRGVECCSYLLAVDVDLNTVPGYAMASWDTGYGD
MAV_3718|M.avium_104 LVGKRMDARLFVDEAAATGVECCGYLLAVDVDMNTVGGYAISGWDTGFGD
Mflv_4065|M.gilvum_PYR-GCK LTGKRISSRLFVDDVAEHGAECCNYLLAVDVDMNTVDGYAISSWEKGYGD
Mvan_1603|M.vanbaalenii_PYR-1 VRGKFVPAEFFLDSVARRGSSIPNIVFGWDIEDTLMDCFTVSGWHTGYSD
MSMEG_1116|M.smegmatis_MC2_155 LLGRRHSWPHYQASAS-KAVNTCEVVFGWGLGHELLDGFSGVGWDHGYGD
. . .. : :: . *
MMAR_3294|M.marinum_M TVAHPDPSTFQVLPWVTDAGHHHSARIFCDITMPDGSPSWADPRHVLRRQ
MUL_1335|M.ulcerans_Agy99 TVAHPDPSTFQVLPWVTDAGHHHSARIFCDITMPDGSPSWADPRHVLRRQ
MLBr_01631|M.leprae_Br4923 TVAHPDPSTFQILPWATTAGHHHSARMFCDITMPDGSSSWADPRHVLRRQ
TH_3396|M.thermoresistible__bu MVARPDPSTFQVLPWTGDSGKHYSARMFCDITMPDGSPSWADPRHVLRRQ
MAB_1920|M.abscessus_ATCC_1997 TVAHPDPSTFQVLPWTTSAGKHHSARMFCDIKLPDGSPSWSDPRHVLRRQ
Mb2885c|M.bovis_AF2122/97 MVMTPDLSTLRLIPWLPG-----TALVIADLVWADGSEVAVSPRSILRRQ
Rv2860c|M.tuberculosis_H37Rv MVMTPDLSTLRLIPWLPG-----TALVIADLVWADGSEVAVSPRSILRRQ
MAV_3718|M.avium_104 LVMRPDLSTLRRIPWLPG-----TALVIADVVGADGSPVAVSPRAVLRRQ
Mflv_4065|M.gilvum_PYR-GCK MVMTPDMSTLRRVPWLPA-----TALVMADLSWTDGRPVTQAPRGILRAQ
Mvan_1603|M.vanbaalenii_PYR-1 VVLAPDLSTLRPVPWEPG-----HAFVLCDVVNTDGSPVPVAPRTVLAGQ
MSMEG_1116|M.smegmatis_MC2_155 LRFVTDPDTLRPMGWQDG-----VGIAFADVVTGDGTPFFASPRNILRKQ
.* .*:: : * . :.*: ** ** :* *
MMAR_3294|M.marinum_M LTKANELGFSCYVHPEIEFFLLNPGP----EDG--TEPVPIDNAGYFDQA
MUL_1335|M.ulcerans_Agy99 LIKANELGFSCYVHPEIEFFLLNPGP----EDG--TEPVPIDNAGYFDQA
MLBr_01631|M.leprae_Br4923 LTKANDLGFSCYVHPEIEFFLLKAGP----EDGSMPIPVPVDNAGYFDQA
TH_3396|M.thermoresistible__bu LAKAGDQGFTCYVHPEIEFFLLKPGP----HDG--SPPVPADTSGYFDQS
MAB_1920|M.abscessus_ATCC_1997 LAKASDLGFSCYVHPEIEFFLLKNLP----DDG--TAPIPADDAGYFDQA
Mb2885c|M.bovis_AF2122/97 LDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAIL
Rv2860c|M.tuberculosis_H37Rv LDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAIL
MAV_3718|M.avium_104 LDRLAGRGLFADAATELEFMVFDEPYRQAWASGYRGLTPASDYNIDYAIS
Mflv_4065|M.gilvum_PYR-GCK LDRLDALGLDAVAATELEFMVFDTGFRDAWAAGYRNLKPATDYNIDYAMH
Mvan_1603|M.vanbaalenii_PYR-1 VERAAALGYRLTAGYELEFYLYRETPETAAAKGYRNLTPASPGTATFSLN
MSMEG_1116|M.smegmatis_MC2_155 IERAHSAGYQPLVTSELEFFALQHTSETLAQQGFTGLKPIRETLQPELAC
: : * . *:** *
MMAR_3294|M.marinum_M VHVSASKFRRHAIDALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADN
MUL_1335|M.ulcerans_Agy99 VHVSASKFRRHVIDALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADN
MLBr_01631|M.leprae_Br4923 VHDSALNFRRHAIEALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADN
TH_3396|M.thermoresistible__bu VHDTAPNFRRHAIEALEQMGISVEFSHHEGAPGQQEIDLRYADALSMADN
MAB_1920|M.abscessus_ATCC_1997 VHEAAPNFRRHAIDALESMGISVEFSHHEGAPGQQEIDLRYADALSMADN
Mb2885c|M.bovis_AF2122/97 ASSRMEPLLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDN
Rv2860c|M.tuberculosis_H37Rv ASSRMEPLLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDN
MAV_3718|M.avium_104 ASSRMEPLLRDIRRGMAGAGLRFESVKGECNRGQQEIGFRYDEALRTCDN
Mflv_4065|M.gilvum_PYR-GCK ASTRMEPLLRDIRLGMDGAGMYCEGVKGECNLGQQEIGFRYEQALVTCDN
Mvan_1603|M.vanbaalenii_PYR-1 RHSALEDVIGAIREGMRACDIPVLASNTEYGAGQIEINIEHADALTTADR
MSMEG_1116|M.smegmatis_MC2_155 AISTDETIMAELFGALQRTGIEVETAKTEYCPGQYEVTLVPTDPMRMADQ
. .: .: : * ** *: : :.: .*.
MMAR_3294|M.marinum_M VMTFRYVIKEVALEEGVRATFMPKPFGQHAGSAMHTHMSLFEGDVN----
MUL_1335|M.ulcerans_Agy99 VMTFRYVIKEVALEEGVRATFMPKPFGQHAGSAMHTHMSLFEGDVN----
MLBr_01631|M.leprae_Br4923 VMTFRYVIKEVAIENGARASFMPKPFAQHPGSAMHTHMSLFEGDVN----
TH_3396|M.thermoresistible__bu VMTFRYVVKQVAISDGVRASFMPKPFAEHPGSAMHTHMSLFEGDTN----
MAB_1920|M.abscessus_ATCC_1997 VMTFRNVVKEVAIIDGVHATFMPKPFSDHPGSAMHTHMSLFEGDTN----
Mb2885c|M.bovis_AF2122/97 HAIYKNGAKEIADQHGKSLTFMAKYD-EREGNSCHIHVSLRGTDGS----
Rv2860c|M.tuberculosis_H37Rv HAIYKNGAKEIADQHGKSLTFMAKYD-EREGNSCHIHVSLRGTDGS----
MAV_3718|M.avium_104 HVIYKNGAKEIADQHGKSLTFMAKYD-EREGNSCHVHLSLRDAQGG----
Mflv_4065|M.gilvum_PYR-GCK HTIYKNGAKEIADQHGKSLTFMAKFD-ESEGNSCHIHISFRGRDGSPEDI
Mvan_1603|M.vanbaalenii_PYR-1 VAIYKNGVRRIAEQHGYLATFMAKVATDSAGSSAHIHQSMQQLDAPAR--
MSMEG_1116|M.smegmatis_MC2_155 HALYKMATRQIFRRHGMSATFMAKVDDHWAGSSSHLHISLTDENGS----
:: :.: .* :**.* . *.: * * *: :
MMAR_3294|M.marinum_M -AFHSPDDPLQLSEVGKSFIAGILEHASEISAVTNQWVNSYKRLILGGEA
MUL_1335|M.ulcerans_Agy99 -AFHSPDDPLQLSEVGKSFIAGILEHASEISAVTNQWVNSYKRLILGGEA
MLBr_01631|M.leprae_Br4923 -AFHSDDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLVVGGEA
TH_3396|M.thermoresistible__bu -AFHSPDDPLQLSDVAKSFIAGILEHANEISAVTNQWVNSYKRLVHGGEA
MAB_1920|M.abscessus_ATCC_1997 -AFHNPDDPLQLSDVGKSFIAGILEHANEISAVTNQWVNSYKRLVQGGEA
Mb2885c|M.bovis_AF2122/97 AVFADSNGPHGMSSMFRSFVAGQLATLREFTLCYAPTINSYKRFADSSFA
Rv2860c|M.tuberculosis_H37Rv AVFADSNGPHGMSSMFRSFVAGQLATLREFTLCYAPTINSYKRFADSSFA
MAV_3718|M.avium_104 AAFADPSRPHGMSTMFCSFLAGLLATMADFTLFYAPNINSYKRFADESFA
Mflv_4065|M.gilvum_PYR-GCK AVFADDSDELGMSSMFRSFIAGQLATLREFTLFYAPNINSYKRFAEGSFA
Mvan_1603|M.vanbaalenii_PYR-1 NAMWDGDRP---SALMRQAVAGQLASMRDFTVLYCPTVNSYKRRVPGSWS
MSMEG_1116|M.smegmatis_MC2_155 NLFAT------DENLLRHFGAGLQAFARDFFLLWAPYPNSYKRFRDGTFA
: . : ** :: ***** :
MMAR_3294|M.marinum_M PTAASWGAANRSALVRVPMYTPHKTSSRRIEVRSPDSACNPYLAFAVLLA
MUL_1335|M.ulcerans_Agy99 PTAASWGAANRSALVRVPMYTPHKTSSRRIEVRSPDSACNPYLAFAVLLA
MLBr_01631|M.leprae_Br4923 PTTASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLAFAVLLA
TH_3396|M.thermoresistible__bu PTAASWGAANRSALVRVPMYTPHKASSRRVEVRSPDSACNPYLTFAVLLA
MAB_1920|M.abscessus_ATCC_1997 PTAASWGAANRSALVRVPMYTPNKSSSRRIEVRSPDSACNPYLTYAVLLA
Mb2885c|M.bovis_AF2122/97 PTALAWGLDNRTCALRVVGHGQN----IRVECRVPGGDVNQYLAVAALIA
Rv2860c|M.tuberculosis_H37Rv PTALAWGLDNRTCALRVVGHGQN----IRVECRVPGGDVNQYLAVAALIA
MAV_3718|M.avium_104 PTALAWGLDNRTCALRVVGHGAH----TRVECRVPGGDVNPYLAVAAIVA
Mflv_4065|M.gilvum_PYR-GCK PTAVAWGLDNRTCSLRVVGHGQG----MRMENRAPGGDVNQYLAVAALIA
Mvan_1603|M.vanbaalenii_PYR-1 PVSAAWGVDNRTAALRVIAHDEAT---CRMENRVPGADANPYLALAACLA
MSMEG_1116|M.smegmatis_MC2_155 PTALTWGDDNRTVALRVTGHGSA----RHLENRIPGADINPYLGYAALLA
*.: :** **: :** : ::* * *.. * ** *. :*
MMAR_3294|M.marinum_M AGLRGVEKGYVLGPQAEDNVWDLTPEERRTMGYRELPTSLDSALRAMESS
MUL_1335|M.ulcerans_Agy99 AGLRGVEKGYVLGPQAEDNVWDLTPEERRTMGYRELPTSLDSALRAMESS
MLBr_01631|M.leprae_Br4923 AGLRGVEKGYVLGPQAEDNVWDLTPEERLAMGYRELPTSLDSALGAMEVS
TH_3396|M.thermoresistible__bu AGLRGVEKGYVLGPEAEDNVWTLTPEERRAMGFKELPSSLGQALAEMERS
MAB_1920|M.abscessus_ATCC_1997 AGLRGVEQGYTLGPEAEDDVWGLTRAERQAMGYKELPSTLENALIAMEGS
Mb2885c|M.bovis_AF2122/97 GGLYGIERGLQLPEPCVG-------NAYQGADVERLPVTLADAAVLFEDS
Rv2860c|M.tuberculosis_H37Rv GGLYGIERGLQLPEPCVG-------NAYQGADVERLPVTLADAAVLFEDS
MAV_3718|M.avium_104 GGLYGIEQGLALPEPCAG-------NAYRARGVGRLPATLAEAAALFEHS
Mflv_4065|M.gilvum_PYR-GCK GGLYGIENKLELEDAVEG-------NAYT-SGADRLPTTLSEAVELFENS
Mvan_1603|M.vanbaalenii_PYR-1 GVLHGIENQLEPPAAVTG-------NAYQGADSP-LPRSLAEAVDAFGAS
MSMEG_1116|M.smegmatis_MC2_155 AGLEGIERGLLPADEVGQR------NAYTEKIGRPLPESLGEALDAFYDS
. * *:*. ** :* .* : *
MMAR_3294|M.marinum_M ELVAEALGEHVFDFFLRNKRREWATYRSHVTPYELSTYLSL-
MUL_1335|M.ulcerans_Agy99 ELVAEALGEHVFDFFLRNKRREWATYRSHVTPYELSTYLSL-
MLBr_01631|M.leprae_Br4923 EFVAEALGEHVFDFFLRNKRAEWANYRSHVSLYELKTYLSL-
TH_3396|M.thermoresistible__bu ELVAEALGEHVFDFFLRNKRAEWENYRSHVTPYELKNYLSL-
MAB_1920|M.abscessus_ATCC_1997 ELVAEALGEHVFDFFLRNKRAEWARYRSNVTPFELRAYLGL-
Mb2885c|M.bovis_AF2122/97 ALVREAFGEDVVAHYLNNARVELAAFNAAVTDWERIRGFERL
Rv2860c|M.tuberculosis_H37Rv ALVREAFGEDVVAHYLNNARVELAAFNAAVTDWERIRGFERL
MAV_3718|M.avium_104 ALARQVFGDDVVAHYLNNARVELAAFHAAVTDWERMRGFERL
Mflv_4065|M.gilvum_PYR-GCK KIARDAFGDDVVDHYVNNARVELKAFNAAVTDWERVRGFERL
Mvan_1603|M.vanbaalenii_PYR-1 SLARTAFGDAFVDHYLASRGWERDRHREAVSDWETGRYFSRV
MSMEG_1116|M.smegmatis_MC2_155 PVTRQTLGEAAVEHITTFLSSELRASQLAVTDWDRRRLFDL-
.. .:*: . . * . *: :: :