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MAFAEYQNELYDQSLHGNQPQYPIRFEELEAKASAAMTPKVLGYVAGGAGDEQTQRANCEAFKRWGLYPR MGIAPEQRDMSVELFGTRFPSPIFMAPIGVIGVCDPDGHGDLACARASIRTGVPFFVGTLSADPMEDLAD ELGDTPAFFQLYTPPDRKMAASLVHRAEAAGFKGIAVTLDTWVTGWRPRDLSGGNYPQVPSGCLANYTSD PVFRAGLSRGEDPTEAAVRKLPIFGGPFRWEDLEWLRSRTSLPLMAKGICHPDDVRRAKDIGVDAIYCSN HGGRQANGGLPCLDCLPGVVEAADGLPVLFDSGVRSGADIVKALALGATAVGIGRPYAYGLALGGVDGIV HVLRSLLAEADLIMAVDGYPSLKDLTPDALRRVEHVAPQRYS
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_4345 | - | - | 100% (392) | lactate 2-monooxygenase |
| M. avium 104 | MAV_3747 | - | e-166 | 72.47% (385) | lactate 2-monooxygenase |
| M. avium 104 | MAV_4477 | - | 6e-40 | 33.91% (345) | FMN-dependent dehydrogenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0713 | lldD1 | 5e-38 | 33.33% (342) | L-lactate dehydrogenase (cytochrome) LldD1 |
| M. gilvum PYR-GCK | Mflv_4136 | - | 1e-153 | 68.57% (385) | FMN-dependent alpha-hydroxy acid dehydrogenase |
| M. tuberculosis H37Rv | Rv0694 | lldD1 | 5e-38 | 33.33% (342) | L-lactate dehydrogenase (cytochrome) LldD1 |
| M. leprae Br4923 | MLBr_02046 | lldD2 | 2e-36 | 29.78% (366) | L-lactate dehydrogenase |
| M. abscessus ATCC 19977 | MAB_2126 | - | 1e-93 | 48.57% (385) | putative L-lactate 2-monooxygenase |
| M. marinum M | MMAR_1816 | lldD1_1 | 1e-164 | 72.21% (385) | L-lactate dehydrogenase (cytochrome) LldD1_1 |
| M. smegmatis MC2 155 | MSMEG_2512 | - | 1e-158 | 69.95% (386) | lactate 2-monooxygenase |
| M. thermoresistible (build 8) | TH_0845 | - | 1e-160 | 70.91% (385) | lactate 2-monooxygenase |
| M. ulcerans Agy99 | MUL_0774 | lldD1 | 8e-38 | 33.82% (343) | L-lactate dehydrogenase (cytochrome) LldD1 |
| M. vanbaalenii PYR-1 | Mvan_2206 | - | 1e-157 | 70.65% (385) | FMN-dependent alpha-hydroxy acid dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_4136|M.gilvum_PYR-GCK -----------MTFGNYQLEIYLQGLSGIMPAYPMDFAGWEARAQASMPP
Mvan_2206|M.vanbaalenii_PYR-1 -----------MTFGNYQLEIYLQGLSGILPNLPMDYAGWEAKAESAMPP
MSMEG_2512|M.smegmatis_MC2_155 -----------MAYGDYQLEIYLQGLAGVLPTMPMDYATLESKAQHAMPA
TH_0845|M.thermoresistible__bu -----------MAFGDYQLEIYLQGLSGVVPSLPMAFAELEARARAAMSP
MMAR_1816|M.marinum_M -----------MAFGDYQYEIYFQGLSGVVPGLPMAFAELESRAQMALPP
MAV_4345|M.avium_104 -----------MAFAEYQNELYDQSLHGNQPQYPIRFEELEAKASAAMTP
MAB_2126|M.abscessus_ATCC_1997 -----------MNFGDFQLQFYAAGALGKVSTLPFNFAELESRAEQTLGE
Mb0713|M.bovis_AF2122/97 -------------------------MAEAWFETVAIAQQR---AKRRLPK
Rv0694|M.tuberculosis_H37Rv -------------------------MAEAWFETVAIAQQR---AKRRLPK
MUL_0774|M.ulcerans_Agy99 -------------------------MADEWFETVAIAQQR---AKRRLPK
MLBr_02046|M.leprae_Br4923 MAAKRRMPRVHDLAPLIQFKRPKFGATKRRLEAALTIQDLRCIAKQRTPK
*
Mflv_4136|M.gilvum_PYR-GCK SVWSYVAGGAGDENTQRANRTAFDRWGLMPRMLVGTTERDLTVDVFGLTL
Mvan_2206|M.vanbaalenii_PYR-1 SVWSYVVGGAGDEHTQRANRTAFDRWGLIPRMLVGATERDLTVDLFGMTL
MSMEG_2512|M.smegmatis_MC2_155 SIWSYVAGGAGDERTQRVNRTAFDRWGLVPRMLNAQRERDLSVDLFGLQL
TH_0845|M.thermoresistible__bu SVWSYVAGGAGDERTQRINVSAFDNWGLIPRMLVGATERDLSVNLFGLTL
MMAR_1816|M.marinum_M SVWSYVVGGAGDERTQRANCEAFDRWGLMPRMFVGAAERDLTVEMFGLTL
MAV_4345|M.avium_104 KVLGYVAGGAGDEQTQRANCEAFKRWGLYPRMGIAPEQRDMSVELFGTRF
MAB_2126|M.abscessus_ATCC_1997 GIFGYVRGGAGDEHTQDSNVTALRRYGLVPRMLRDRTVRDMSTSFLGRTF
Mb0713|M.bovis_AF2122/97 SVYSSLIAASEKGITVADNVAAFSELGFAPHVIGATDKRDLSTTVMGQEV
Rv0694|M.tuberculosis_H37Rv SVYSSLIAASEKGITVADNVAAFSELGFAPHVIGATDKRDLSTTVMGQEV
MUL_0774|M.ulcerans_Agy99 SVYSSLISASEKGITVADNVAAFSELGFAPHVIGAAEKRDMSTTVMGQDI
MLBr_02046|M.leprae_Br4923 AAFDYTDGAAEDELSIKRAQQAFRDIEFHPAILRDVTNVCAGWDVLGHSV
. ..: . : *: : * : .:* .
Mflv_4136|M.gilvum_PYR-GCK PSPIFMAPVGVAGICSQSGHGDLEAARAAARTGVPMAVSTLTEDPLEDVA
Mvan_2206|M.vanbaalenii_PYR-1 PSPLFMAPIGVIGICSQNGQGDLAAARAAARTGVPMTVSTLTEDPLEDVA
MSMEG_2512|M.smegmatis_MC2_155 PSPLFMAPIGVLGICGQDGHGDLAGAQAAARTGVPMVVSTLTQDPLENVA
TH_0845|M.thermoresistible__bu PSPIFMAPIGVLGICAQDHHGDLACARAAARTGVPLVVSTLTQDPLEDIA
MMAR_1816|M.marinum_M PSPIFLAPIGVIGLCAQDGHGDLATARAAARTGVPMVVSTLTADPMEDVA
MAV_4345|M.avium_104 PSPIFMAPIGVIGVCDPDGHGDLACARASIRTGVPFFVGTLSADPMEDLA
MAB_2126|M.abscessus_ATCC_1997 TSPAFICPVGVLGAVRD--RGDLLTAAAARELDMPAMYSTLSAATLEEVA
Mb0713|M.bovis_AF2122/97 SLPVIISPTG-VQAVDP--GGEVAVARAAAARGTVMGLSSFASKPIEEVI
Rv0694|M.tuberculosis_H37Rv SLPVIISPTG-VQAVDP--GGEVAVARAAAARGTVMGLSSFASKPIEEVI
MUL_0774|M.ulcerans_Agy99 SMPVLISPTG-VQAVHP--DGEVAVARAAAARGTAMGLSSFASKTIEDVI
MLBr_02046|M.leprae_Br4923 LLPFGIAPTGFTRLMHT--EGEIAGARAAAAAGIPFSLSTLATSAIEDVV
* :.* * *:: * *: . .::: .:*::
Mflv_4136|M.gilvum_PYR-GCK AEFGDTPGFFQLYTPTDRDLAASFVQRAEAAGYKAIIVTLDTWIPGWRPR
Mvan_2206|M.vanbaalenii_PYR-1 AEFGDTPGFFQLYTPTDRDLAASLVHRAEAAGYKAIIVTLDTWIPGWRPR
MSMEG_2512|M.smegmatis_MC2_155 AQFGDTPGFFQLYTPTDRDLAASFVRRAEAAGYKAIVVTLDTWVPGWRPR
TH_0845|M.thermoresistible__bu AEFGDTPGFFQLYTPNDRDLAASLVRRAEKAGYKGIIVTLDTWIPGWRPR
MMAR_1816|M.marinum_M AEFGDIPGFFQLYTPKDRELAASLVQRAESAGFKGIVVTLDTWIPGWRPR
MAV_4345|M.avium_104 DELGDTPAFFQLYTPPDRKMAASLVHRAEAAGFKGIAVTLDTWVTGWRPR
MAB_2126|M.abscessus_ATCC_1997 AARGDSYGIFQLYPSSDSELTDNFIRRAEAAGYDALAVTLDTGTLGWRPR
Mb0713|M.bovis_AF2122/97 AANP--KTFFQVYWQGGRDALAERVERARQAGAVGLVVTTDWTFSHGRDW
Rv0694|M.tuberculosis_H37Rv AANP--KTFFQVYWQGGRDALAERVERARQAGAVGLVVTTDWTFSHGRDW
MUL_0774|M.ulcerans_Agy99 AANP--KIFFQIYWLGGRDAIAERVERARQAGAVGLIVTTDWTFSHGRDW
MLBr_02046|M.leprae_Br4923 AAVPQGRKWFQLYMWRDRDRSMALVERAADAGYDALLVTVDVPVAGARLR
**:* . . :.** ** .: ** * *
Mflv_4136|M.gilvum_PYR-GCK DLSTS-------NFPQLRGRCLSNYTSDPVFR--GLLPQPPEENMQA---
Mvan_2206|M.vanbaalenii_PYR-1 DLAMS-------NFPQLRGRCLANYTSDPVFR--AALSQPPEENMQA---
MSMEG_2512|M.smegmatis_MC2_155 DLSTS-------NFPQLRGKCLANYTSDPVFR--AGLPQPPEENPQA---
TH_0845|M.thermoresistible__bu DLSTS-------NFPQLRGYSLANYTSDPVFS--AGLQQPPEQNPQA---
MMAR_1816|M.marinum_M DLSTA-------NFPQLRGHCLSNYTSDPVFR--AGLPRPPEEDPQG---
MAV_4345|M.avium_104 DLSGG-------NYPQVPSGCLANYTSDPVFR--AGLSR--GEDPTE---
MAB_2126|M.abscessus_ATCC_1997 DLKHG-------YLPMLHGHCLANYTSDPRFLEIAGVRSAGELTPMH---
Mb0713|M.bovis_AF2122/97 GSPK---IPEEMNLKTILRLSPEAITRPRWLWKFAKTLRPPDLRVPNQGR
Rv0694|M.tuberculosis_H37Rv GSPK---IPEEMNLKTILRLSPEAITRPRWLWKFAKTLRPPDLRVPNQGR
MUL_0774|M.ulcerans_Agy99 GSPK---IPEQMNLRTILRLSPEAIVRPRWLWKFGKTLRPPDLRVPNQGR
MLBr_02046|M.leprae_Br4923 DTRNGMSIPPALTLRTVF----DALPHPRWWIDLLTTEP---LAFASLDR
. :
Mflv_4136|M.gilvum_PYR-GCK -------TVLQWAGMFGNA-LSWDDLPWLRSLTDLPLILKGLCHPDDVRR
Mvan_2206|M.vanbaalenii_PYR-1 -------AVLQWVAQFGNA-LTWEDLPWLRSLTDLPLVLKGLCHPDDVRR
MSMEG_2512|M.smegmatis_MC2_155 -------TVLRWVSQFGNP-LTWADLPWLRSLTKLPLILKGICHPDDVRR
TH_0845|M.thermoresistible__bu -------VVTRWVQLFGNS-LTWDDLPWLRSLTTLPLILKGICHPDDVRR
MMAR_1816|M.marinum_M -------TVLRWAQLFGNP-LTWSDLAWLRELTDLPLIVKGICHPDDARR
MAV_4345|M.avium_104 -------AAVRKLPIFGGP-FRWEDLEWLRSRTSLPLMAKGICHPDDVRR
MAB_2126|M.abscessus_ATCC_1997 -------AGLVWASLFSHPGLTWADIDHYRSITKLPIILKGICDSDDVRQ
Mb0713|M.bovis_AF2122/97 RGEPGPPFFAAYGEWMATPPPTWEDIGWLRELWGGPFMLKGVMRVDDAKR
Rv0694|M.tuberculosis_H37Rv RGEPGPPFFAAYGEWMATPPPTWEDIGWLRELWGGPFMLKGVMRVDDAKR
MUL_0774|M.ulcerans_Agy99 RGEPGPAFFAAYGEWMGTPPPTWDDIAWLRELWGGPFMLKGVMRVDDAKR
MLBr_02046|M.leprae_Br4923 WSG----TVSDYLNTMFDPSVTFDDLAWIKTQWPGKFVVKGIQTLDDARA
: . : *: : :: **: **.:
Mflv_4136|M.gilvum_PYR-GCK ARDGGVDGIYCSTHGGRQANGGLPAIDCLPVVVEAADG-LPVLFDSGVRS
Mvan_2206|M.vanbaalenii_PYR-1 AKDAGVDGVYCSTHGGRQANGGLPALDCLPGVVEAADG-LPVLFDSGIRS
MSMEG_2512|M.smegmatis_MC2_155 AKDEGVDGIYCSNHGGRQANGGVPAIDCLPGVVEAADG-LPVLFDSGIRN
TH_0845|M.thermoresistible__bu AKDAGVDGIYCSNHGGRQANGGLPAIDCLPDVVAAADG-MPVLFDSGIRS
MMAR_1816|M.marinum_M AKDGGVDGIYCSTHGGRQANGGLPALDCLPGVVEAADG-LPVLFDSGIRS
MAV_4345|M.avium_104 AKDIGVDAIYCSNHGGRQANGGLPCLDCLPGVVEAADG-LPVLFDSGVRS
MAB_2126|M.abscessus_ATCC_1997 AVDRGVDAIAYSNHGGRQANGGVPAIDGLAAAVEAAGS-VPVTFDSGIRD
Mb0713|M.bovis_AF2122/97 AVDAGVSAISVSNHGGNNLDGTPASIRALPAVSAAVGDQVEVLLDGGIRR
Rv0694|M.tuberculosis_H37Rv AVDAGVSAISVSNHGGNNLDGTPASIRALPAVSAAVGDQVEVLLDGGIRR
MUL_0774|M.ulcerans_Agy99 AVDAGVSAISVSNHGGNNLDGTPASIRALPAVAAAVGDQVEVLLDGGIRR
MLBr_02046|M.leprae_Br4923 VVERGIDGVVLSNHGGRQLDRAPVPFHLLPTVAREFGKDTEILLDTGIMS
. : *:..: *.***.: : : *. . . : :* *:
Mflv_4136|M.gilvum_PYR-GCK GADIVKALALGATAVGIGRPYAYGLALGGTDGLVHVLRSLLAETDLIMAV
Mvan_2206|M.vanbaalenii_PYR-1 GADIVKALALGATAVGVGRPYAYGLAIGGEDGIVHVLRSLLAEADLIMAV
MSMEG_2512|M.smegmatis_MC2_155 GADIVKALALGATAVGVGRPYVFGLALGGVDGIVHVLRSLLAEADLIMAV
TH_0845|M.thermoresistible__bu GADIVKALALGATAVGIGRPYPYGLALGGEDGVVHVLRSLLAEADLIMAV
MMAR_1816|M.marinum_M GADIIKALAMGATAVGIGRPYAYGLALGGVDGVVHVLRMLLAEADLIMAV
MAV_4345|M.avium_104 GADIVKALALGATAVGIGRPYAYGLALGGVDGIVHVLRSLLAEADLIMAV
MAB_2126|M.abscessus_ATCC_1997 GIDILRAVALGASLVGVARPYVYGLALDGTNGVKHVIQSLLAEADLTMAV
Mb0713|M.bovis_AF2122/97 GSDVVKAVALGARAVMIGRAYLWGLAANGQAGVENVLDILRGGIDSALMG
Rv0694|M.tuberculosis_H37Rv GSDVVKAVALGARAVMIGRAYLWGLAANGQAGVENVLDILRGGIDSALMG
MUL_0774|M.ulcerans_Agy99 GSDVVKAVALGARAVLVGRAYLWGLAANGQAGVENVLDILRGGIDSALMG
MLBr_02046|M.leprae_Br4923 GADIVAAIALGARCTLVGRAYLYGLMAGGEAGVRRAIEILESGVIRTMQL
* *:: *:*:** . :.*.* :** .* *: ..: * . :
Mflv_4136|M.gilvum_PYR-GCK DGYPTLADLTPDTVRRVT--------------
Mvan_2206|M.vanbaalenii_PYR-1 DGYRSLADLTPDTLRRVI--------------
MSMEG_2512|M.smegmatis_MC2_155 DGYPSLADLTPDTLRRVDA-------------
TH_0845|M.thermoresistible__bu DGYPTLADLTPDALRRVT--------------
MMAR_1816|M.marinum_M DGYPTRKDLTPDTLRRVN--------------
MAV_4345|M.avium_104 DGYPSLKDLTPDALRRVEHVAPQRYS------
MAB_2126|M.abscessus_ATCC_1997 NCYLSLNELAVQRLS-----------------
Mb0713|M.bovis_AF2122/97 LGHASVHDLSPADILVPTGFIRDLGVPSRRDV
Rv0694|M.tuberculosis_H37Rv LGHASVHDLSPADILVPTGFIRDLGVPSRRDV
MUL_0774|M.ulcerans_Agy99 LGHSSIHDLRSDDILIPADFVRRLGR------
MLBr_02046|M.leprae_Br4923 LGVTCLEELSPR----HVTQLQRLGPIEPPA-
:*