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MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGSGITEASFVRA LLDAQQLESTIAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIGMIEIDSENRLFERVAERLRS IGVGKLRSSLLNAFSITVDEQKYEHLNTQIERCRQQGISLIRINDSVGTLVPDSTAVLAANLVRDNPDIT FYLHGHNDRGLGTANSLTSVFHGFQMIEGGVAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRA ARLSEDVFLTAPDPFRAVSGFLTENENAGIVNVPDYLGVSRSVKYFLNRIGLFPTYVRQILSESEFDSST VDDDSFIDQVYERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVRDCALEVLRDRGSAHAKVAS
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. abscessus ATCC 19977 | MAB_3553 | - | - | 100% (419) | 2-isopropylmalate synthase |
| M. abscessus ATCC 19977 | MAB_0626 | - | 6e-09 | 22.78% (259) | 4-hydroxy-2-ketovalerate aldolase |
| M. abscessus ATCC 19977 | MAB_4650c | - | 3e-05 | 25.00% (124) | putative pyruvate carboxyltransferase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3498c | mhpE | 2e-14 | 23.88% (289) | 4-hydroxy-2-ketovalerate aldolase |
| M. gilvum PYR-GCK | Mflv_1522 | - | 2e-07 | 24.12% (257) | 4-hydroxy-2-ketovalerate aldolase |
| M. tuberculosis H37Rv | Rv3469c | mhpE | 2e-14 | 23.88% (289) | 4-hydroxy-2-ketovalerate aldolase |
| M. leprae Br4923 | - | - | - | - | - |
| M. marinum M | MMAR_5021 | - | 2e-08 | 32.69% (104) | 4-hydroxy-2-oxovalerate aldolase |
| M. avium 104 | MAV_0627 | - | 2e-07 | 24.51% (257) | 4-hydroxy-2-ketovalerate aldolase |
| M. smegmatis MC2 155 | MSMEG_2024 | - | 7e-10 | 23.29% (249) | hydroxymethylglutaryl-CoA lyase |
| M. thermoresistible (build 8) | TH_0371 | - | 2e-07 | 24.90% (253) | PROBABLE 4-HYDROXY-2-OXOVALERATE ALDOLASE (HOA) |
| M. ulcerans Agy99 | MUL_4095 | - | 1e-08 | 32.69% (104) | 4-hydroxy-2-ketovalerate aldolase |
| M. vanbaalenii PYR-1 | Mvan_5234 | - | 5e-08 | 24.90% (257) | 4-hydroxy-2-ketovalerate aldolase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1522|M.gilvum_PYR-GCK ----MSTEDIYFNPIWDVRMTDTSLRDGSHHKRHQFTKDEVGSIVAALDA
Mvan_5234|M.vanbaalenii_PYR-1 ----MSTDEIYFNPIWDVRMTDTSLRDGSHHKRHQFTKDEVQAIVAALDA
MAV_0627|M.avium_104 -----MTTDIFFNPMWDVRLTDTSLRDGSHHKRHQFTKDEVQAIVAALDV
TH_0371|M.thermoresistible__bu -----MTTDIFFNPVWDVRITDTSLRDGSHHKRHQFTKDEVRSIVAALDT
MMAR_5021|M.marinum_M -----------MTGIWDVRITDTSLRDGSHHKRHQFIKEEVGAIVAALDA
MUL_4095|M.ulcerans_Agy99 -----------MTGIWDVRITDTSLRDGSHHKRHQFIKEEVGAIVAALDA
Mb3498c|M.bovis_AF2122/97 --------MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDA
Rv3469c|M.tuberculosis_H37Rv --------MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDA
MAB_3553|M.abscessus_ATCC_1997 MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITD
MSMEG_2024|M.smegmatis_MC2_155 -----MTVREPSPYTPRAELRDVTLRDGLQLTAKTLPTELKIEVVRELLR
: * ::*:* : :: :
Mflv_1522|M.gilvum_PYR-GCK AGVPVIEVT---HGDGLGGSSFNYGFSKTPEQELIKLAAETAKESKIAFL
Mvan_5234|M.vanbaalenii_PYR-1 AGVPVIEVT---HGDGLGGSSFNYGFSKTPEQELIKLAAETAKESKIAFL
MAV_0627|M.avium_104 AGVPVIEVT---HGDGLGGSSFNYGFSKTPEQELIKLAAETAKEAKIAFL
TH_0371|M.thermoresistible__bu AGVPVIEVT---HGDGLGGSSFNYGFSKTPEQELIKLAAETAKESKIAFL
MMAR_5021|M.marinum_M AGVPVIEVT---HGDGLGGSSFNYGFSKTPEQELIKLAAQTAKEAKIAFL
MUL_4095|M.ulcerans_Agy99 AGVPVIEVT---HGDGLGGSSFNYGFSKTSEQELIKLAAQTAKEAKIAFL
Mb3498c|M.bovis_AF2122/97 AGIPYIEIG---HGVTIG-AAAAQGPAAHTDEEYFRAARSVVRNARLGAV
Rv3469c|M.tuberculosis_H37Rv AGIPYIEIG---HGVTIG-AAAAQGPAAHTDEEYFRAARSVVRNARLGAV
MAB_3553|M.abscessus_ATCC_1997 AGITDIDVG---SGITEASFVRALLDAQQLESTIAPDATFSFNLTLKTWE
MSMEG_2024|M.smegmatis_MC2_155 LGVPALEIGSMARADLVPTLADTLDVVAALTPEELDRCWIWVATPRHVEK
*:. ::: . . .
Mflv_1522|M.gilvum_PYR-GCK MLPGVGTKEDIKEAQNNGGSICRIATHCTEADVSIQHFGLARELGLETVG
Mvan_5234|M.vanbaalenii_PYR-1 MLPGVGTKEDIKEAQSNGGSICRIATHCTEADVSIQHFGLARELGLETVG
MAV_0627|M.avium_104 MLPGVGTKEDIKEAQNNGGSICRIATHCTEADVSIQHFGLARELGLETVG
TH_0371|M.thermoresistible__bu MLPGVGTKDDIKEAQDNGGSICRIATHCTEADVSIQHFGLARELGLETVG
MMAR_5021|M.marinum_M MLPGVGTKEDIKEAQDNGGSICRIATHCTEADVSIQHFGLARELGLETVG
MUL_4095|M.ulcerans_Agy99 MLPGVGTKEDIKEAQDNGGSICRIATHCTEADVSIQHLGLARELGLETVG
Mb3498c|M.bovis_AF2122/97 IVPALARIETVDLAG-DYLDFLRICVIATEFELVMPFVERAQSKGLEVSI
Rv3469c|M.tuberculosis_H37Rv IVPALARIETVDLAG-DYLDFLRICVIATEFELVMPFVERAQSKGLEVSI
MAB_3553|M.abscessus_ATCC_1997 PLCEQLERELPSSYLSDIYVSIGMIEIDSENRLFERVAERLRSIGVGKLR
MSMEG_2024|M.smegmatis_MC2_155 AAAAGARKFQYCLSASDAHNKANIGRDTETSVAAMPDAIRIAHEAGGEIQ
: : .
Mflv_1522|M.gilvum_PYR-GCK -FLMMSHTISPEK------LAKQARIMADAGCQCVYVVDSAGALVLEGVA
Mvan_5234|M.vanbaalenii_PYR-1 -FLMMSHTIPPEK------LAKQARIMADAGCQCVYVVDSAGALVLEGVA
MAV_0627|M.avium_104 -FLMMSHTIPPEK------LAQQARIMADAGCQCVYVVDSAGALVLEGVR
TH_0371|M.thermoresistible__bu -FLMMAHTIPPEK------LAEQARIMADAGCQCVYVVDSAGALVLEGVR
MMAR_5021|M.marinum_M -FLMMAHTIAPEK------LAAQARIMADAGCQCVYVVDSAGALVLDGVA
MUL_4095|M.ulcerans_Agy99 -FLMMAHTIAPEK------LAAQARIMADAGCQCVYVVDSAGALVLDGVA
Mb3498c|M.bovis_AF2122/97 -QLVKSHLFEPDV------LAAAGKRARDVGVRIVYVVDTTGTFLPEDAR
Rv3469c|M.tuberculosis_H37Rv -QLVKSHLFEPDV------LAAAGKRARDVGVRIVYVVDTTGTFLPEDAR
MAB_3553|M.abscessus_ATCC_1997 SSLLNAFSITVDEQKY-EHLNTQIERCRQQGISLIRINDSVGTLVPDSTA
MSMEG_2024|M.smegmatis_MC2_155 LCIATAFTCPFDGLVDPQRVWGIAADPRTSGAVDIVVCDTIGQAIPTQVS
: :. : : * : : *: * : .
Mflv_1522|M.gilvum_PYR-GCK DRVSALVAELGDDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGSCRRFG
Mvan_5234|M.vanbaalenii_PYR-1 DRVAALVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGSCRRFG
MAV_0627|M.avium_104 DRVAALVAELGDDAQVGFHGHENLGLGVANSVEAVRAGAKQIDGSCRRFG
TH_0371|M.thermoresistible__bu DRVAALVAELGDDAQVGFHGHENLGLGVANSVEAVRAGAKQIDGSCRRFG
MMAR_5021|M.marinum_M DRVAALVAELGEDAQVGFHGHENLGLGVANSVEAVRAGAKQIDGSVRRFG
MUL_4095|M.ulcerans_Agy99 DRVAALVAELGEDAQVGFHGHENLGLGVANSVEAVRAGAKQIDGSVRRFG
Mb3498c|M.bovis_AF2122/97 RYVEALRGAS--DVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFG
Rv3469c|M.tuberculosis_H37Rv RYVEALRGAS--DVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFG
MAB_3553|M.abscessus_ATCC_1997 VLAANLVRDN-PDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGGVAGVG
MSMEG_2024|M.smegmatis_MC2_155 ALIHGLHPVN-PERRIVFHGHDTWGLGVANTLAAIDAGATMVDGSLGGLG
* : . :***: .:..**:: :. * ::* .*
Mflv_1522|M.gilvum_PYR-GCK AGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRN
Mvan_5234|M.vanbaalenii_PYR-1 AGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRN
MAV_0627|M.avium_104 AGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRN
TH_0371|M.thermoresistible__bu AGAGNAPVEALIGVFDKVGVKTGIDFFDIADAAEEVVAPAMPSECLLDRN
MMAR_5021|M.marinum_M AGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRN
MUL_4095|M.ulcerans_Agy99 AGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRN
Mb3498c|M.bovis_AF2122/97 RGAGNCQIECLVAALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPW
Rv3469c|M.tuberculosis_H37Rv RGAGNCQIECLVAALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPW
MAB_3553|M.abscessus_ATCC_1997 NRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPF
MSMEG_2024|M.smegmatis_MC2_155 -GCPFAPGASGNTSSEDLLFALRPDWLTPSAFESLVLLSEKILAELGEPN
. . . . :
Mflv_1522|M.gilvum_PYR-GCK ALIMGYS----------------------------GVYSSFLKHAIRQSE
Mvan_5234|M.vanbaalenii_PYR-1 ALIMGYS----------------------------GVYSSFLKHAIRQSE
MAV_0627|M.avium_104 ALIMGYS----------------------------GVYSSFLKHAIRQSE
TH_0371|M.thermoresistible__bu ALIMGYS----------------------------GVYSSFLKHAIRQSE
MMAR_5021|M.marinum_M ALIMGYS----------------------------GVYSSFLKHAVRQSE
MUL_4095|M.ulcerans_Agy99 ALIMGYS----------------------------GVYSSFLKHAVRQSE
Mb3498c|M.bovis_AF2122/97 EISFGFH----------------------------GLDSLQVEHLRAAAQ
Rv3469c|M.tuberculosis_H37Rv EISFGFH----------------------------GLDSLQVEHLRAAAQ
MAB_3553|M.abscessus_ATCC_1997 RAVSGFLTENENAGIVNVPDYLGVSRSVKYFLNRIGLFPTYVRQILSESE
MSMEG_2024|M.smegmatis_MC2_155 RSKAAQG-------------------------------------ARSKAR
. :.
Mflv_1522|M.gilvum_PYR-GCK RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
Mvan_5234|M.vanbaalenii_PYR-1 RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
MAV_0627|M.avium_104 RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
TH_0371|M.thermoresistible__bu RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
MMAR_5021|M.marinum_M RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
MUL_4095|M.ulcerans_Agy99 RYG-------------------VPAHQLLHRAGQRKLIGGQE--------
Mb3498c|M.bovis_AF2122/97 QAG-------------------LSVSHVIRQTAKSHAGQWLS--------
Rv3469c|M.tuberculosis_H37Rv QAG-------------------LSVSHVIRQTAKSHAGQWLS--------
MAB_3553|M.abscessus_ATCC_1997 FDSSTVDDDSFIDQVYERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVR
MSMEG_2024|M.smegmatis_MC2_155 AFG-----------------------WVIDA-------------------
. :
Mflv_1522|M.gilvum_PYR-GCK -DQLIDIALEIKREQEAEATRA--
Mvan_5234|M.vanbaalenii_PYR-1 -DQLIDIALEIKREQETAKS----
MAV_0627|M.avium_104 -DQLIDIALEIKREQESGAAAAR-
TH_0371|M.thermoresistible__bu -DQLIDIALEIKRELEEQAGSTA-
MMAR_5021|M.marinum_M -DQLIDIALEIKREQESGQVASRR
MUL_4095|M.ulcerans_Agy99 -DQLIDIALEIKREQESGQVASRR
Mb3498c|M.bovis_AF2122/97 -PQDIDRVVVGMRA----------
Rv3469c|M.tuberculosis_H37Rv -PQDIDRVVVGMRA----------
MAB_3553|M.abscessus_ATCC_1997 LDDVRDCALEVLRDRGSAHAKVAS
MSMEG_2024|M.smegmatis_MC2_155 ------------------------