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M. thermoresistible (build 8) TH_1329 (murF)

annotation: Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase MurF
coordinates: 2525830 - 2527482
length: 551

aa seq not available

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. thermoresistible (build 8) TH_1329 murF - 100% (551) Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase MurF
M. thermoresistible (build 8) TH_1476 - 6e-10 31.37% (153) Probable UDP-N-acetylmuramate-alanine ligase MurC
M. thermoresistible (build 8) TH_2285 - 2e-08 27.34% (256) POSSIBLE LIGASE

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb2181c murF 1e-152 72.59% (394) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino
M. gilvum PYR-GCK Mflv_2984 - 1e-157 62.73% (499) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
M. tuberculosis H37Rv Rv2157c murF 1e-152 72.59% (394) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-
M. leprae Br4923 MLBr_00910 murF 1e-145 67.34% (395) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
M. abscessus ATCC 19977 MAB_2002 - 1e-144 69.97% (393) UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase MurF
M. marinum M MMAR_3197 murF 1e-154 65.30% (464) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
M. avium 104 MAV_2332 - 1e-159 75.06% (397) UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
M. smegmatis MC2 155 MSMEG_4231 - 1e-166 78.66% (389) UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
M. ulcerans Agy99 MUL_3505 murF 1e-153 70.47% (403) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-
M. vanbaalenii PYR-1 Mvan_3527 - 1e-157 63.53% (499) UDP-N-acetylmuramoylalanyl-D-glutamyl-2,

CLUSTAL 2.0.9 multiple sequence alignment


MMAR_3197|M.marinum_M               --------MIDYTVAEIAEIVGGTLVDISPQEAEQRRVTGTVEFDSRAVG
MUL_3505|M.ulcerans_Agy99           --------MIDYTVAEIAEIVGGTLVDISPQEAEQRRVTGTVEFDSRAVG
Mb2181c|M.bovis_AF2122/97           --------MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIG
Rv2157c|M.tuberculosis_H37Rv        --------MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIG
MAV_2332|M.avium_104                --------MIDLTVAQIADIVGGTLADITAPDAARRRVTGTVEFDSRAVG
MLBr_00910|M.leprae_Br4923          --------MIDLTVARVTEIVGGALVDISPEEAAERRVTGSVEFDSRAVG
MAB_2002|M.abscessus_ATCC_1997      -----------MTVAEIAEIVGGRLDGISAQEARQARVTGTVEFDSRQVS
Mflv_2984|M.gilvum_PYR-GCK          --------MIEMSLRRIAEIVGGRLADVSADEADRLRVTGTVEFDSRAVT
Mvan_3527|M.vanbaalenii_PYR-1       --------MIAMSLARIAEIVGGRLADVSDEDAARTVVTGTVEFDSRAVT
MSMEG_4231|M.smegmatis_MC2_155      --------MIDMTIARIAEIVGGELADITPEQAAATHVTGTVEFDSRAVG
TH_1329|M.thermoresistible__bu      VTTEPGAVVSDLGQAARRSAVRTPVTAGSGGGYPRLDLTACPGLVVVAAR
                                                      . *   :   :        :*.   :      

MMAR_3197|M.marinum_M               PGGLFLALPGARADGHDH-----AASAVAAGAVAVLAARPVG---VP---
MUL_3505|M.ulcerans_Agy99           PGGLFLALPGARADGHDH-----AAAAVAAGAVAVLAARPVG---VS---
Mb2181c|M.bovis_AF2122/97           PGGLFLALPGARADGHDH-----AASAVAAGAAVVLAARPVG---VP---
Rv2157c|M.tuberculosis_H37Rv        PGGLFLALPGARADGHDH-----AASAVAAGAAVVLAARPVG---VP---
MAV_2332|M.avium_104                PGGLFLALPGARTDGHDH-----AESAVAAGAVAVLAARPVG---VP---
MLBr_00910|M.leprae_Br4923          PGGLFLALPGARSDGHDH-----AASAIAAGAVAVLAARPVG---VP---
MAB_2002|M.abscessus_ATCC_1997      PGGLFLALPGARADGHDH-----ARGAAEAGAVAVLAARPVG---VP---
Mflv_2984|M.gilvum_PYR-GCK          AGGLFLALPGARSDGHDF-----AATAVASGAVAVLAARPVG---VP---
Mvan_3527|M.vanbaalenii_PYR-1       PGGLFLALPGARSDGHDF-----AATAVAAGAVGVLAARPVG---VP---
MSMEG_4231|M.smegmatis_MC2_155      PGGLFLALPGARSDGHDF-----AAAAVQSGAVAVLAARPVG---VP---
TH_1329|M.thermoresistible__bu      PDDELLGLGGAIAALVDLGVEVRVVSVCDRDATLTLTGLPALDRQIPDQR
                                    ... :*.* ** :   *      .  .   .*. .*:. *.    :.   

MMAR_3197|M.marinum_M               ----------------AIVVSPE-PDVTADALTGVLEHDADGSGAAVLAA
MUL_3505|M.ulcerans_Agy99           ----------------AIVVSPE-PDSTADALTGVLEHDADGSGAAVLAA
Mb2181c|M.bovis_AF2122/97           ----------------AIVVPP---VAAPNVLAGVLEHDNDGSGAAVLAA
Rv2157c|M.tuberculosis_H37Rv        ----------------AIVVPP---VAAPNVLAGVLEHDNDGSGAAVLAA
MAV_2332|M.avium_104                ----------------AIVAPP---VDAADALAGVLEHDADGSGAAVLAA
MLBr_00910|M.leprae_Br4923          ----------------AIVVAPD-PRTGDGGLTGVLEHDADGSGAAVLAA
MAB_2002|M.abscessus_ATCC_1997      ----------------AIVVPP---TGVARTEVSALEHDSDGSGAAVLAA
Mflv_2984|M.gilvum_PYR-GCK          ----------------AVVVDPVAPQHGAGGTAGVLEHDADGSGAAVLAA
Mvan_3527|M.vanbaalenii_PYR-1       ----------------AVVVDPVRPDPAAPGTAGVLEHDVDGSGAAVLAA
MSMEG_4231|M.smegmatis_MC2_155      ----------------AIVVRP---DGGVDSASGALEHDTDGSGAAVLAA
TH_1329|M.thermoresistible__bu      FDQRVAAEILGVKELHHLGLPADDLDRHAAGLADDLTELLVGPGLWCAAP
                                                     :   .           . * .   *.*    *.

MMAR_3197|M.marinum_M               LAK--------LAKAVAAELVAGGLTIIGITGSSGKTSTKDLVAAVLKPL
MUL_3505|M.ulcerans_Agy99           LAK--------LAKAVAAELMAGGLTIIGITGSSGKTSTKDLVAAVLKPL
Mb2181c|M.bovis_AF2122/97           LAK--------LATAVAAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPL
Rv2157c|M.tuberculosis_H37Rv        LAK--------LATAVAAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPL
MAV_2332|M.avium_104                LAK--------LARAVAAELVAGGLTIVGITGSSGKTSTKDLVAAVLAPL
MLBr_00910|M.leprae_Br4923          LAK--------LAKAVAAELVAGGLTIIGITGSSGKTSTKDLVAVVLELL
MAB_2002|M.abscessus_ATCC_1997      TAA--------LAAEVARRLTARGLRIVGITGSSGKTSTKDLIAAVLSPL
Mflv_2984|M.gilvum_PYR-GCK          LAR--------LAAAVAAELVAGGLTIVGITGSSGKTSTKDLIAAVLAPL
Mvan_3527|M.vanbaalenii_PYR-1       LAR--------LASAVAAELVAGGLTIIGITGSSGKTSTKDLIAAVLAPL
MSMEG_4231|M.smegmatis_MC2_155      LAR--------LAAAVANELAADGLKIIGVTGSSGKTSTKDLLAAVLAPL
TH_1329|M.thermoresistible__bu      WAGDGDPDSTVVARAAXXXLVAGGLRIVGVTGSSGKTSTKDLLAAVLAPL
                                     *         :*  .   * * ** *:*:************:*.**  *

MMAR_3197|M.marinum_M               GEVVAPPGSFNNELGHPWTVLRATRGTDYLILEMSARHPGNIAALARVAP
MUL_3505|M.ulcerans_Agy99           GEVVAPPGSFNNELGHPWTVLRATRSTDYLILEMSARHPGNIAALARVAP
Mb2181c|M.bovis_AF2122/97           GEVVAPPGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAP
Rv2157c|M.tuberculosis_H37Rv        GEVVAPPGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAP
MAV_2332|M.avium_104                GEVVAPPGSFNNELGHPWTVLRATADTDYLVLEMSARHPGNIAALAEIAP
MLBr_00910|M.leprae_Br4923          GEVVAPPESFNNELGHPWTVLRATRSTDYLILEMSARRPGNIAALAAIAP
MAB_2002|M.abscessus_ATCC_1997      GEVVAPPGSFNNELGHPWTVLRATESTKFLVLELSARRPGNIAELAAIAP
Mflv_2984|M.gilvum_PYR-GCK          GEVIAPPGSFNNELGHPWTVLRATESTDYLVLEMSARHPGNIAALARIAP
Mvan_3527|M.vanbaalenii_PYR-1       GEVVAPPGSFNNELGHPWTVLRATRSTDYLVLEMSARHPGNIAALARIAV
MSMEG_4231|M.smegmatis_MC2_155      GEVVAPPGSFNNELGHPWTVLRATRDTDFLVLEMSARHPGNIAALAAIAP
TH_1329|M.thermoresistible__bu      GSVVAPPGSFNNELGHPWTVLRADRDTDYLVLEMSARHRGNIAALAEIAP
                                    *.*:*** ***************   *.:*:**::**: **** ** :* 

MMAR_3197|M.marinum_M               PAIGVVLNVGTAHLGEFGSREIIAQTKSELPQAVPASGVVILNADDPAVA
MUL_3505|M.ulcerans_Agy99           PAIGVVLNVGTAHLGEFGSREIIAQTKSELPQAVPASGVVILNADDPAVA
Mb2181c|M.bovis_AF2122/97           PSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPHSGAVVLNADDPAVA
Rv2157c|M.tuberculosis_H37Rv        PSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPHSGAVVLNADDPAVA
MAV_2332|M.avium_104                PAVGVVLNVGTAHLGEFGSREAIARTKSELPQSVPASGVVVLNVDDPTVA
MLBr_00910|M.leprae_Br4923          PKIGVVLNVGTAHLGEFGSREAIARTKTELPQAIMQSGVVILNVDDPAVA
MAB_2002|M.abscessus_ATCC_1997      PSIGVVLNVGTAHLGEFGSRQAIADTKGELPQSLSGGGVAVLNADDAAVA
Mflv_2984|M.gilvum_PYR-GCK          PSIGAVLNVGTAHLGEFGSREAIAATKSELPQAVPQSGVVILNADDPVVA
Mvan_3527|M.vanbaalenii_PYR-1       PSIAVVLNVGTAHLGEFGSREVIAATKSELPQSVPESGVVILNADDPAVA
MSMEG_4231|M.smegmatis_MC2_155      PSVAVVLNVGTAHLGEFGSREAIAKTKAELPQAVGPSGVVILNADDIAVA
TH_1329|M.thermoresistible__bu      PSVGVVLNVGTAHLGEFGSRDAIAATKAELAEAVPSTGLVVLNADDPAVA
                                    * :..***************: ** ** **.:::   * .:**.** .**

MMAR_3197|M.marinum_M               AMAEVTAARVVRVGRATATGAGWAGPGDVWAGPVSLDRLARPQFTMHAGD
MUL_3505|M.ulcerans_Agy99           AMAEVTAARVVRVGRATATGAGWAGPGDVWAGPVSLDRLARPQFTMHAGD
Mb2181c|M.bovis_AF2122/97           AMAKLTAARVVRVSRDN--------TGDVWAGPVSLDELARPRFTLHAHD
Rv2157c|M.tuberculosis_H37Rv        AMAKLTAARVVRVSRDN--------TGDVWAGPVSLDELARPRFTLHAHD
MAV_2332|M.avium_104                AMAEVTAARVVRVSRAEHTEA---GPSDVWAGPVSLDELARPGFTLYHSH
MLBr_00910|M.leprae_Br4923          AMADATVARVIRVSRGSYSHPGSPNSPDVWTGPVSLDELARPRFTLHTRD
MAB_2002|M.abscessus_ATCC_1997      AMAAKTPARVVRTSVHH--------PADIWATEVSVDDLARARFTLHAGQ
Mflv_2984|M.gilvum_PYR-GCK          AMADKTAARVVRVGRSADAR-----TLDVRADGVTLDPLARPAFTLHAGG
Mvan_3527|M.vanbaalenii_PYR-1       AMADKTAARVVRVGRGAEA-------LDVCADGVSLDALARPSFTLHTGA
MSMEG_4231|M.smegmatis_MC2_155      AMAEQTDARVVRISRDPGS------DIDVWAGPVTLDELARPRFTLHTRD
TH_1329|M.thermoresistible__bu      AMAERTAARVVRVAQDPGER-----QVDVWAERVSLDELARPRFTLRTPD
                                    ***  * ***:* .             *: :  *::* ***. **:    

MMAR_3197|M.marinum_M               A----AAQVRLGVYGDHQVSNALCAGAVALECGASVSQVAAALAEAGPVS
MUL_3505|M.ulcerans_Agy99           A----AAQVRLGVYGDHQVSNALCAGAVALECGASVSQVAAALAEAGPVS
Mb2181c|M.bovis_AF2122/97           A----QAEVRLGVCGDHQVTNALCAAAVALECGASVEQVAAALTAAPPVS
Rv2157c|M.tuberculosis_H37Rv        A----QAEVRLGVCGDHQVTNALCAAAVALECGASVEQVAAALTAAPPVS
MAV_2332|M.avium_104                AGGTRQARVQLGVSGDHQVGNALCAAAVALECGATVEQVVAALAGAGPVS
MLBr_00910|M.leprae_Br4923          PRAG-ATEIQLGVYGDHQVANALCAAAVGLECGASVEEVAVALAATGPVS
MAB_2002|M.abscessus_ATCC_1997      D----SVPVQLAVHGEHHVSNALAAAAVALECGATLAQVAAALAGAGPVS
Mflv_2984|M.gilvum_PYR-GCK          E----QVPVTLAVHGDHQVSNALVAAAVALECGATLTQVAGALAAAGPVS
Mvan_3527|M.vanbaalenii_PYR-1       A----DVPVTLAVHGDHQVSNALCAAAVALECGATPEQVAAALATAGPVS
MSMEG_4231|M.smegmatis_MC2_155      G----EVDVNLAVHGDHQVSNALCAAAVALECGATPQQVADALAAAGPAS
TH_1329|M.thermoresistible__bu      G----AVEVALTVHGEHQVSNALAAAAVALHCGATLEQVVAALAGAGPAS
                                          . : * * *:*:* *** *.**.*.***:  :*. **: : *.*

MMAR_3197|M.marinum_M               RHRMQVSTRPDGVTVIDDAYNANPDSMRAGLQALAWIARGEAESTAASQP
MUL_3505|M.ulcerans_Agy99           RHRMRVSTRPDGVTVIDDAYNANPDSMRAGLQALAWIARGEAESTAASQP
Mb2181c|M.bovis_AF2122/97           RHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAH---QP------
Rv2157c|M.tuberculosis_H37Rv        RHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAH---QP------
MAV_2332|M.avium_104                RHRMQVSTRGDGVTVIDDAYNANPDSMRAGLQALAWIARGGAEPG-----
MLBr_00910|M.leprae_Br4923          RHRMQVTTRADGVTVIDDTYNANPDSMRAGLQALAWIAHGGTHDKNQP--
MAB_2002|M.abscessus_ATCC_1997      RRRMEVSTRDDGVTVINDAYNANPDSMMAGLKALVSMARHGGEP------
Mflv_2984|M.gilvum_PYR-GCK          RHRMQVSTRSDGVTVINDAYNANPDSMTAGLKALAWMSRQNPEG------
Mvan_3527|M.vanbaalenii_PYR-1       RHRMQVTAREDGVTVINDAYNANPDSMRAGLKALAWMSRGGASK------
MSMEG_4231|M.smegmatis_MC2_155      RHRMQVSTRADGVTVINDAYNANPDSMRAGLKALAWMARQTAGGQP----
TH_1329|M.thermoresistible__bu      PRRMQVSTRSDGVTVINDAYNANPDSMRAALKSLVWMAKAGGRAG-----
                                     :**.*::* ******:*:******** *.*::*. :::           

MMAR_3197|M.marinum_M               GVRPQSRRSWAVLGEMAELGADAITEHDHIGRLAVRLDVSRLVVVGTGRS
MUL_3505|M.ulcerans_Agy99           GVRPQSRRSWAVLGEMAELGADAITEHDHIGRLAVRLDVSRLVVVGTGRS
Mb2181c|M.bovis_AF2122/97           ---EATRRSWAVLGEMAELGEDAIAEHDRIGRLAVRLDVSRLVVVGTGRS
Rv2157c|M.tuberculosis_H37Rv        ---EATRRSWAVLGEMAELGEDAIAEHDRIGRLAVRLDVSRLVVVGTGRS
MAV_2332|M.avium_104                ---APARRSWAVLGEMAELGDDAIAEHDRIGRLAVRLDVSRLVVVGTGRS
MLBr_00910|M.leprae_Br4923          --GSCARRSWAVLGEMAELGEDSITEHDRIGRLAVRLDVSRLVVVGGGRS
MAB_2002|M.abscessus_ATCC_1997      ------RRSWAVLGEMAELGPDAISEHDRIGRAAVRLDVSRLVVVGTGRS
Mflv_2984|M.gilvum_PYR-GCK          ------SRSFAVLGEMAELGEDTITEHDRIGRLAVRLDVSRLVVVGSGRP
Mvan_3527|M.vanbaalenii_PYR-1       ------GRSWAVLGEMAELGDDAITEHDRIGRLAVRLDVSRLIVVGSGRP
MSMEG_4231|M.smegmatis_MC2_155      ---GGKRRSWAVLGEMAELGDDAITEHDAIGRFAVRLDVSRLLVVGSGRA
TH_1329|M.thermoresistible__bu      ---GPPRRSWAVLGEMAELGDDAITEHDNIGRLAVRLDVSRLIVVGTGRA
                                           **:********** *:*:*** *** *********:*** **.

MMAR_3197|M.marinum_M               MSAMYHGAVMEGSWGT-----------EAVSVPDRDAALALLRAEIQPGD
MUL_3505|M.ulcerans_Agy99           MSAIYHGAVMEGSWGT-----------EAVSVPDRDAALALLRAEIQPGD
Mb2181c|M.bovis_AF2122/97           ISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
Rv2157c|M.tuberculosis_H37Rv        ISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
MAV_2332|M.avium_104                VSAMHHGAVMEGSWGS--------ADKGAVNVPDAEAALELLRAELQPGD
MLBr_00910|M.leprae_Br4923          INAMHRGAVMEGSWGL-----------ETVNVADPAAALTLLRAEVRPGD
MAB_2002|M.abscessus_ATCC_1997      QRAMHAGAVMEGSWGS-----------EAVLVDDPAAAASLLASELAAGD
Mflv_2984|M.gilvum_PYR-GCK          MSAMLHGAVMEGSWGS-----------EATPVADADAALALLRDELRPGD
Mvan_3527|M.vanbaalenii_PYR-1       MSAMLHGAVMEGSFGS-----------EAIPVADTDAALEILRAELRPGD
MSMEG_4231|M.smegmatis_MC2_155      MNAMHHGAVMEGSWGS-----------ESTMVADADAALTVLRDELQPGD
TH_1329|M.thermoresistible__bu      MSAMHHGAVMEGSWGA-----------ESTMVSDADAALRLLRSELQAGD
                                      *:  ***:**::*             :  * *  **  :*  *: .**

MMAR_3197|M.marinum_M               VVLIKASNSAGLGELADALACADAG--------------CQDRGSAHS--
MUL_3505|M.ulcerans_Agy99           VVLIKASNSAGLGELADALACADAG--------------CQDRGSAHS--
Mb2181c|M.bovis_AF2122/97           VVLVKASNAAGLGAVADALVADD------------------TCGSVRP--
Rv2157c|M.tuberculosis_H37Rv        VVLVKASNAAGLGAVADALVADD------------------TCGSVRP--
MAV_2332|M.avium_104                VVLVKASNAAGLGALADALARDDREGGAAGRSGSSPLARDDSAGGTRR--
MLBr_00910|M.leprae_Br4923          VVLIKASHSVGLGALADVLVGDG---------------------AIRP--
MAB_2002|M.abscessus_ATCC_1997      VVLVKASRSAGLWVVADELLKVG---------------------DA----
Mflv_2984|M.gilvum_PYR-GCK          VVLVKASNSAGLGALADALVS-----------------------EQLPGD
Mvan_3527|M.vanbaalenii_PYR-1       VVLVKASNSAGLGALADALVSDVARDGVSDVARDGVSDVARDGVSEPPRD
MSMEG_4231|M.smegmatis_MC2_155      VVLVKASNSVGLGALAEALVAGEG----------------HTEGRTPAR-
TH_1329|M.thermoresistible__bu      VVLVKASNAAGLGALADALAAES--------------------GDTGR--
                                    ***:***.:.**  :*: *                               

MMAR_3197|M.marinum_M               ---
MUL_3505|M.ulcerans_Agy99           ---
Mb2181c|M.bovis_AF2122/97           ---
Rv2157c|M.tuberculosis_H37Rv        ---
MAV_2332|M.avium_104                ---
MLBr_00910|M.leprae_Br4923          ---
MAB_2002|M.abscessus_ATCC_1997      ---
Mflv_2984|M.gilvum_PYR-GCK          GR-
Mvan_3527|M.vanbaalenii_PYR-1       GGR
MSMEG_4231|M.smegmatis_MC2_155      ---
TH_1329|M.thermoresistible__bu      ---

species	id	gene	e-value	identity (len)	annotation
M. thermoresistible (build 8)	TH_1329	murF	-	100% (551)	Probable UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase MurF
M. thermoresistible (build 8)	TH_1476	-	6e-10	31.37% (153)	Probable UDP-N-acetylmuramate-alanine ligase MurC
M. thermoresistible (build 8)	TH_2285	-	2e-08	27.34% (256)	POSSIBLE LIGASE