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M. tuberculosis H37Rv Rv3356c (folD)

annotation: PROBABLE BIFUNCTIONAL PROTEIN FOLD: METHYLENETETRAHYDROFOLATE DEHYDROGENASE + METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE
coordinates: 3769804 - 3770649
length: 281

GAIMLDGKATRDEIFGDLKQRVAALDAAGRTPGLGTILVGDDPGSQAYVRGKHADCAKVGITSIRRDLPA
DISTATLNETIDELNANPDCTGYIVQLPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCT
PRGIVHLLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLPALTRQADIVVAAV
GVAHLLTADMVRPGAAVIDVGVSRTDDGLVGDVHPDVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR
*

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. tuberculosis H37Rv Rv3356c folD - 100% (281) PROBABLE BIFUNCTIONAL PROTEIN FOLD: METHYLENETETRAHYDROFOLATE DEHYDROGENASE + METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3391c folD 1e-161 99.64% (281) bifunctional methylenetetrahydrofolate
M. gilvum PYR-GCK Mflv_4879 - 1e-137 84.64% (280) methylenetetrahydrofolate dehydrogenase (NADP(+))
M. leprae Br4923 MLBr_00674 folD 1e-141 86.83% (281) bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/
M. abscessus ATCC 19977 MAB_3692c - 1e-135 83.93% (280) bifunctional 5,10-methylene-tetrahydrofolate
M. marinum M MMAR_1176 folD 1e-149 91.10% (281) bifunctional protein: methylenetetrahydrofolate
M. avium 104 MAV_4323 - 1e-149 93.21% (280) tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding
M. smegmatis MC2 155 MSMEG_1647 - 1e-143 87.54% (281) tetrahydrofolate dehydrogenase/cyclohydrolase FolD
M. thermoresistible (build 8) TH_1967 folD 1e-139 85.05% (281) PROBABLE BIFUNCTIONAL PROTEIN FOLD:
M. ulcerans Agy99 MUL_1428 folD 1e-148 90.75% (281) bifunctional protein: methylenetetrahydrofolate
M. vanbaalenii PYR-1 Mvan_1550 - 1e-137 85.36% (280) methylenetetrahydrofolate dehydrogenase (NADP(+))

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_4879|M.gilvum_PYR-GCK          MMRVGAITLDGKATRDEIFVDLRERVARLAAAGRTPGLATVLVGDDPGSH
Mvan_1550|M.vanbaalenii_PYR-1       MMRVGAITLDGKATRDEIFVDLKERVTALTAAGRTPGLGTVLVGDDPGSQ
TH_1967|M.thermoresistible__bu      ---VGAIRLDGKATRDEIFAELKERVAALTAAGRTPGLGTVLVGDDPGSH
Rv3356c|M.tuberculosis_H37Rv        ---MGAIMLDGKATRDEIFGDLKQRVAALDAAGRTPGLGTILVGDDPGSQ
Mb3391c|M.bovis_AF2122/97           ---MGAIMLDGKATRDEIFGDLKPRVAALDAAGRTPGLGTILVGDDPGSQ
MAV_4323|M.avium_104                ---MGAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQ
MLBr_00674|M.leprae_Br4923          ---MGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSH
MMAR_1176|M.marinum_M               ---MGAITLDGKATRDEIFVDLKQRVSELNASGRTPGLATILVGEDPGSQ
MUL_1428|M.ulcerans_Agy99           ---MGAITLDGKATRDEIFVDLKQRVSELNASGRTPGLATILVGEDPGSQ
MSMEG_1647|M.smegmatis_MC2_155      ---MGAISLDGKTTRDEIFVDLKERVAALTAAGRTPGLGTVLVGDDPGSQ
MAB_3692c|M.abscessus_ATCC_199      ---MTATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSH
                                       : *  ****:*****: :*  *.: *  :*:****.*:***:****:

Mflv_4879|M.gilvum_PYR-GCK          AYVRGKHADCAKVGINSIRRDLPADISQAVLDETIDELNANPECTGYIVQ
Mvan_1550|M.vanbaalenii_PYR-1       AYVRGKHADCAKVGIESIRRDLPADISQETLLETLDELNANPACTGYIVQ
TH_1967|M.thermoresistible__bu      AYVRGKHSDCAKVGITSIRRDLPADISQAELADVLDELNADPACTGYIVQ
Rv3356c|M.tuberculosis_H37Rv        AYVRGKHADCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
Mb3391c|M.bovis_AF2122/97           AYVRGKHADCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MAV_4323|M.avium_104                AYVRGKHADCAKVGITSIRRDLPADISTAALNDTIDELNANPECTGYIVQ
MLBr_00674|M.leprae_Br4923          AYVRGKHADCAKVGITSIRRDLPVDITTAVLHDTIEELNANPDCTGYIVQ
MMAR_1176|M.marinum_M               AYVRGKHSDCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MUL_1428|M.ulcerans_Agy99           AYVRGKHSDCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MSMEG_1647|M.smegmatis_MC2_155      AYVRGKHADCAKVGINSIRRDLPADITTEQLNETIDELNANPDCTGYIVQ
MAB_3692c|M.abscessus_ATCC_199      AYVRGKHADCAKVGLNSIRRDLPADIDQATLCATIDELNANPDCTGYIVQ
                                    *******:******: *******.**    *  .::****:* *******

Mflv_4879|M.gilvum_PYR-GCK          LPLPKHLNENAALERIDPSKDADGLHPTNLGRLVLNEPAPLPCTPRGIVH
Mvan_1550|M.vanbaalenii_PYR-1       LPLPKHLNENAALERIDPGKDADGLHPMNLGRLVLNEPAALPCTPRGIVH
TH_1967|M.thermoresistible__bu      LPLPGHLDENAALERIDPAKDADGLHPMNLGRLVLNEPAPLPCTPRGIVH
Rv3356c|M.tuberculosis_H37Rv        LPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCTPRGIVH
Mb3391c|M.bovis_AF2122/97           LPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCTPRGIVH
MAV_4323|M.avium_104                LPLPKQLDENAALERVDPDKDADGLHPTNLGRLVLNNPAPLPCTPRGIVH
MLBr_00674|M.leprae_Br4923          LPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILH
MMAR_1176|M.marinum_M               LPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNTPAPLPCTPRGIVH
MUL_1428|M.ulcerans_Agy99           LPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNTPAPLPCTPRGIVH
MSMEG_1647|M.smegmatis_MC2_155      LPLPKHLDENAALERIDPAKDADGLHPTNLGRLVLGKQAALPCTPRGIVH
MAB_3692c|M.abscessus_ATCC_199      LPLPKHLDENEALERIDPDKDADGLHPTNLGRLVLGKEAPLPCTPRGIVY
                                    ****  *:** ****:** ******** *******.  *.****.***::

Mflv_4879|M.gilvum_PYR-GCK          LLRRFEVEIAGAHVAVLGRGVTVGRPLGLLLTRRSENATVTLCHTATRHL
Mvan_1550|M.vanbaalenii_PYR-1       LLRRYQVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHL
TH_1967|M.thermoresistible__bu      LLRRFDVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNL
Rv3356c|M.tuberculosis_H37Rv        LLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
Mb3391c|M.bovis_AF2122/97           LLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MAV_4323|M.avium_104                LLRRYDVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MLBr_00674|M.leprae_Br4923          LLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNL
MMAR_1176|M.marinum_M               LLRRYDVPIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MUL_1428|M.ulcerans_Agy99           LLRRYDVPIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MSMEG_1647|M.smegmatis_MC2_155      LLRRFDVPIAGAHVVVIGRGVTVGRPMGLLLTRRSENATVTLCHTGTRDL
MAB_3692c|M.abscessus_ATCC_199      LLRRYDVKLDGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
                                    ****: : : *:**.::*********:******************.**.*

Mflv_4879|M.gilvum_PYR-GCK          PEITREADIIVAAAGVPHMVTADMVRPGAAVVDVGVSRDDAGKLVGDVHP
Mvan_1550|M.vanbaalenii_PYR-1       PQITREADIIIAAAGVPHMVTAEMVRPGAAVVDVGVSRDEAGKLVGDVHP
TH_1967|M.thermoresistible__bu      PEIARQADIIIAAAGVPHMVTADMVRPGAAVVDVGVSRDEQGKLVGDVHP
Rv3356c|M.tuberculosis_H37Rv        PALTRQADIVVAAVGVAHLLTADMVRPGAAVIDVGVSRTDD-GLVGDVHP
Mb3391c|M.bovis_AF2122/97           PALTRQADIVVAAVGVAHLLTADMVRPGAAVIDVGVSRTDD-GLVGDVHP
MAV_4323|M.avium_104                PALTRQADIVVAAVGVPHLLTADMVRPGAAVLDVGVSRVDG-KLAGDVHP
MLBr_00674|M.leprae_Br4923          AALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVET-RLVGDVHP
MMAR_1176|M.marinum_M               PTLTRQADIIVAAVGVPHMLTADMVRPGAAVVDVGVSRVDG-KLTGDVHP
MUL_1428|M.ulcerans_Agy99           ATLTRQADIIVAAVGVPHMLTADMVRPGAAVVDVGVSRVDG-KLTGDVHP
MSMEG_1647|M.smegmatis_MC2_155      PALTRQADIIIAAVGVPHMVTADMVKPGAAVVDVGVSRVDG-KLTGDVAP
MAB_3692c|M.abscessus_ATCC_199      AALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDD-KLTGDVAE
                                    . ::::***::**.**.*::**:**:***.*:****** :   *.***  

Mflv_4879|M.gilvum_PYR-GCK          GVWDVAGHVSPNPGGVGPLTRAFLLTNVVERAEANL--
Mvan_1550|M.vanbaalenii_PYR-1       DVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALAGS
TH_1967|M.thermoresistible__bu      DVWDVAGHVSPNPGGVGPLTRAFLLTNVVELAEKA---
Rv3356c|M.tuberculosis_H37Rv        DVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR---
Mb3391c|M.bovis_AF2122/97           DVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR---
MAV_4323|M.avium_104                DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE---
MLBr_00674|M.leprae_Br4923          DVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ--
MMAR_1176|M.marinum_M               DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAEQR---
MUL_1428|M.ulcerans_Agy99           DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAEQR---
MSMEG_1647|M.smegmatis_MC2_155      DVWEVAGHVSPNPGGVGPLTRAFLLTNVVEAEESKLA-
MAB_3692c|M.abscessus_ATCC_199      DVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS---
                                    .**::**:*************.******:*  *

species	id	gene	e-value	identity (len)	annotation
M. tuberculosis H37Rv	Rv3356c	folD	-	100% (281)	PROBABLE BIFUNCTIONAL PROTEIN FOLD: METHYLENETETRAHYDROFOLATE DEHYDROGENASE + METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE