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M. leprae Br4923 MLBr_00674 (folD)

annotation: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase
coordinates: 810701 - 811549
length: 282

VGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSHAYVRGKHADCAKVGITSIRRDLP
VDITTAVLHDTIEELNANPDCTGYIVQLPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPC
TARGILHLLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNLAALTKQADIIVAA
VGVPHLLTADMVRPGAVVVDVGVSRVETRLVGDVHPDVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEG
RQ

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. leprae Br4923 MLBr_00674 folD - 100% (282) bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3391c folD 1e-140 86.48% (281) bifunctional methylenetetrahydrofolate
M. gilvum PYR-GCK Mflv_4879 - 1e-133 81.07% (280) methylenetetrahydrofolate dehydrogenase (NADP(+))
M. tuberculosis H37Rv Rv3356c folD 1e-141 86.83% (281) bifunctional methylenetetrahydrofolate
M. abscessus ATCC 19977 MAB_3692c - 1e-129 79.93% (279) bifunctional 5,10-methylene-tetrahydrofolate
M. marinum M MMAR_1176 folD 1e-141 86.12% (281) bifunctional protein: methylenetetrahydrofolate
M. avium 104 MAV_4323 - 1e-143 88.53% (279) tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding
M. smegmatis MC2 155 MSMEG_1647 - 1e-136 82.56% (281) tetrahydrofolate dehydrogenase/cyclohydrolase FolD
M. thermoresistible (build 8) TH_1967 folD 1e-134 82.86% (280) PROBABLE BIFUNCTIONAL PROTEIN FOLD:
M. ulcerans Agy99 MUL_1428 folD 1e-142 86.48% (281) bifunctional protein: methylenetetrahydrofolate
M. vanbaalenii PYR-1 Mvan_1550 - 1e-132 81.43% (280) methylenetetrahydrofolate dehydrogenase (NADP(+))

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_4879|M.gilvum_PYR-GCK          MMRVGAITLDGKATRDEIFVDLRERVARLAAAGRTPGLATVLVGDDPGSH
Mvan_1550|M.vanbaalenii_PYR-1       MMRVGAITLDGKATRDEIFVDLKERVTALTAAGRTPGLGTVLVGDDPGSQ
TH_1967|M.thermoresistible__bu      ---VGAIRLDGKATRDEIFAELKERVAALTAAGRTPGLGTVLVGDDPGSH
Mb3391c|M.bovis_AF2122/97           ---MGAIMLDGKATRDEIFGDLKPRVAALDAAGRTPGLGTILVGDDPGSQ
Rv3356c|M.tuberculosis_H37Rv        ---MGAIMLDGKATRDEIFGDLKQRVAALDAAGRTPGLGTILVGDDPGSQ
MAV_4323|M.avium_104                ---MGAITLDGKATRDEIFVDLKQRVAALTAAGRTPGLGTILVGDDPGSQ
MLBr_00674|M.leprae_Br4923          ---MGAITLDGKATRDEILIDLKQRVAALTESGRTPGLGTILVGDDPGSH
MMAR_1176|M.marinum_M               ---MGAITLDGKATRDEIFVDLKQRVSELNASGRTPGLATILVGEDPGSQ
MUL_1428|M.ulcerans_Agy99           ---MGAITLDGKATRDEIFVDLKQRVSELNASGRTPGLATILVGEDPGSQ
MSMEG_1647|M.smegmatis_MC2_155      ---MGAISLDGKTTRDEIFVDLKERVAALTAAGRTPGLGTVLVGDDPGSQ
MAB_3692c|M.abscessus_ATCC_199      ---MTATRLDGKATRDEIFVELATRAAALKAAGKTPGLGTILVGDDPGSH
                                       : *  ****:*****: :*  *.: *  :*:****.*:***:****:

Mflv_4879|M.gilvum_PYR-GCK          AYVRGKHADCAKVGINSIRRDLPADISQAVLDETIDELNANPECTGYIVQ
Mvan_1550|M.vanbaalenii_PYR-1       AYVRGKHADCAKVGIESIRRDLPADISQETLLETLDELNANPACTGYIVQ
TH_1967|M.thermoresistible__bu      AYVRGKHSDCAKVGITSIRRDLPADISQAELADVLDELNADPACTGYIVQ
Mb3391c|M.bovis_AF2122/97           AYVRGKHADCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
Rv3356c|M.tuberculosis_H37Rv        AYVRGKHADCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MAV_4323|M.avium_104                AYVRGKHADCAKVGITSIRRDLPADISTAALNDTIDELNANPECTGYIVQ
MLBr_00674|M.leprae_Br4923          AYVRGKHADCAKVGITSIRRDLPVDITTAVLHDTIEELNANPDCTGYIVQ
MMAR_1176|M.marinum_M               AYVRGKHSDCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MUL_1428|M.ulcerans_Agy99           AYVRGKHSDCAKVGITSIRRDLPADISTATLNETIDELNANPDCTGYIVQ
MSMEG_1647|M.smegmatis_MC2_155      AYVRGKHADCAKVGINSIRRDLPADITTEQLNETIDELNANPDCTGYIVQ
MAB_3692c|M.abscessus_ATCC_199      AYVRGKHADCAKVGLNSIRRDLPADIDQATLCATIDELNANPDCTGYIVQ
                                    *******:******: *******.**    *  .::****:* *******

Mflv_4879|M.gilvum_PYR-GCK          LPLPKHLNENAALERIDPSKDADGLHPTNLGRLVLNEPAPLPCTPRGIVH
Mvan_1550|M.vanbaalenii_PYR-1       LPLPKHLNENAALERIDPGKDADGLHPMNLGRLVLNEPAALPCTPRGIVH
TH_1967|M.thermoresistible__bu      LPLPGHLDENAALERIDPAKDADGLHPMNLGRLVLNEPAPLPCTPRGIVH
Mb3391c|M.bovis_AF2122/97           LPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCTPRGIVH
Rv3356c|M.tuberculosis_H37Rv        LPLPKHLDENAALERVDPAKDADGLHPTNLGRLVLGTPAPLPCTPRGIVH
MAV_4323|M.avium_104                LPLPKQLDENAALERVDPDKDADGLHPTNLGRLVLNNPAPLPCTPRGIVH
MLBr_00674|M.leprae_Br4923          LPLPKYLDENTALERVDPAKDADGLHPTNLGRLVLSTPAPLPCTARGILH
MMAR_1176|M.marinum_M               LPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNTPAPLPCTPRGIVH
MUL_1428|M.ulcerans_Agy99           LPLPKHLDENAALERIDPGKDADGLHPTNLGRLVLNTPAPLPCTPRGIVH
MSMEG_1647|M.smegmatis_MC2_155      LPLPKHLDENAALERIDPAKDADGLHPTNLGRLVLGKQAALPCTPRGIVH
MAB_3692c|M.abscessus_ATCC_199      LPLPKHLDENEALERIDPDKDADGLHPTNLGRLVLGKEAPLPCTPRGIVY
                                    ****  *:** ****:** ******** *******.  *.****.***::

Mflv_4879|M.gilvum_PYR-GCK          LLRRFEVEIAGAHVAVLGRGVTVGRPLGLLLTRRSENATVTLCHTATRHL
Mvan_1550|M.vanbaalenii_PYR-1       LLRRYQVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHL
TH_1967|M.thermoresistible__bu      LLRRFDVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNL
Mb3391c|M.bovis_AF2122/97           LLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
Rv3356c|M.tuberculosis_H37Rv        LLRRYDISIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MAV_4323|M.avium_104                LLRRYDVEIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MLBr_00674|M.leprae_Br4923          LLRRYGVEIAGTHVVIIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRNL
MMAR_1176|M.marinum_M               LLRRYDVPIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MUL_1428|M.ulcerans_Agy99           LLRRYDVPIAGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
MSMEG_1647|M.smegmatis_MC2_155      LLRRFDVPIAGAHVVVIGRGVTVGRPMGLLLTRRSENATVTLCHTGTRDL
MAB_3692c|M.abscessus_ATCC_199      LLRRYDVKLDGAHVVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDL
                                    ****: : : *:**.::*********:******************.**.*

Mflv_4879|M.gilvum_PYR-GCK          PEITREADIIVAAAGVPHMVTADMVRPGAAVVDVGVSRDDAGKLVGDVHP
Mvan_1550|M.vanbaalenii_PYR-1       PQITREADIIIAAAGVPHMVTAEMVRPGAAVVDVGVSRDEAGKLVGDVHP
TH_1967|M.thermoresistible__bu      PEIARQADIIIAAAGVPHMVTADMVRPGAAVVDVGVSRDEQGKLVGDVHP
Mb3391c|M.bovis_AF2122/97           PALTRQADIVVAAVGVAHLLTADMVRPGAAVIDVGVSRTDD-GLVGDVHP
Rv3356c|M.tuberculosis_H37Rv        PALTRQADIVVAAVGVAHLLTADMVRPGAAVIDVGVSRTDD-GLVGDVHP
MAV_4323|M.avium_104                PALTRQADIVVAAVGVPHLLTADMVRPGAAVLDVGVSRVDG-KLAGDVHP
MLBr_00674|M.leprae_Br4923          AALTKQADIIVAAVGVPHLLTADMVRPGAVVVDVGVSRVET-RLVGDVHP
MMAR_1176|M.marinum_M               PTLTRQADIIVAAVGVPHMLTADMVRPGAAVVDVGVSRVDG-KLTGDVHP
MUL_1428|M.ulcerans_Agy99           ATLTRQADIIVAAVGVPHMLTADMVRPGAAVVDVGVSRVDG-KLTGDVHP
MSMEG_1647|M.smegmatis_MC2_155      PALTRQADIIIAAVGVPHMVTADMVKPGAAVVDVGVSRVDG-KLTGDVAP
MAB_3692c|M.abscessus_ATCC_199      AALTRQADIIVAAAGVPHMVTADMVKPGAAVVDVGVSRVDD-KLTGDVAE
                                    . ::::***::**.**.*::**:**:***.*:****** :   *.***  

Mflv_4879|M.gilvum_PYR-GCK          GVWDVAGHVSPNPGGVGPLTRAFLLTNVVERAEANL--
Mvan_1550|M.vanbaalenii_PYR-1       DVWEVAGHVSPNPGGVGPLTRAFLLTNVVERAEALAGS
TH_1967|M.thermoresistible__bu      DVWDVAGHVSPNPGGVGPLTRAFLLTNVVELAEKA---
Mb3391c|M.bovis_AF2122/97           DVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR---
Rv3356c|M.tuberculosis_H37Rv        DVWELAGHVSPNPGGVGPLTRAFLLTNVVELAERR---
MAV_4323|M.avium_104                DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAERE---
MLBr_00674|M.leprae_Br4923          DVWEVAGHVSPNPGGVGPLTRVFLLTNVVELAEGRQ--
MMAR_1176|M.marinum_M               DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAEQR---
MUL_1428|M.ulcerans_Agy99           DVWEVAGHVSPNPGGVGPLTRAFLLTNVVELAEQR---
MSMEG_1647|M.smegmatis_MC2_155      DVWEVAGHVSPNPGGVGPLTRAFLLTNVVEAEESKLA-
MAB_3692c|M.abscessus_ATCC_199      DVWDVAGYVSPNPGGVGPLTRAFLLTNVIERAERS---
                                    .**::**:*************.******:*  *

species	id	gene	e-value	identity (len)	annotation
M. leprae Br4923	MLBr_00674	folD	-	100% (282)	bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase