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M. tuberculosis H37Rv Rv2847c (cysG)

annotation: POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN-III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT) (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2 OXIDASE + FERROCHELATASE
coordinates: 3154654 - 3155871
length: 405

TENPYLVGLRLAGKKVVVVGGGTVAQRRLPLLIASGADVHVIAPSVTPAVEAMDQITLSVRDYRDGDLDG
AWYAIAATDDARVNVAVVAEAERRRIFCVRADIAVEGTAVTPASFSYAGLSVGVLAGGEHRRSAAIRSAI
REALQQGVITAQSSDVLSGGVALVGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHVEVID
AAKIPYGRAMAQDAINAVLIERARSGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTSAIAVP
AMAGVPVTHRAMTHEFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGGRTADTP
VLVVQHGTTAAQQTLRATLADTPEKVRAAGIRPPAIIVIGAVVGLSGVRGLNNS*

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. tuberculosis H37Rv Rv2847c cysG - 100% (405) POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN-III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT) (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2 OXIDASE + FERROCHELATASE
M. tuberculosis H37Rv Rv2071c cobM 2e-24 34.16% (243) precorrin-4 C11-methyltransferase CobM
M. tuberculosis H37Rv Rv0511 hemD 5e-19 29.50% (261) uroporphyrin-III C-methyltransferase HemD

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb2872c cysG 0.0 100.00% (405) multifunctional uroporphyrinogen III methylase/precorrin-2
M. gilvum PYR-GCK Mflv_4047 - 0.0 82.07% (396) uroporphyrin-III C-methyltransferase
M. leprae Br4923 MLBr_02420 hemD 3e-19 28.87% (284) putative uroporphyrin-III C-methyltransferase
M. abscessus ATCC 19977 MAB_3143c - 1e-172 78.88% (393) multifunctional siroheme synthase CysG/uroporphyrin-III
M. marinum M MMAR_1886 cysG 0.0 85.35% (396) multifunctional enzyme siroheme synthase CysG
M. avium 104 MAV_3703 cobA 0.0 88.64% (396) uroporphyrin-III C-methyltransferase
M. smegmatis MC2 155 MSMEG_2618 cobA 0.0 80.85% (402) uroporphyrin-III C-methyltransferase
M. thermoresistible (build 8) TH_1981 cysG2 0.0 80.68% (409) POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG:
M. ulcerans Agy99 MUL_2114 cysG 0.0 85.10% (396) multifunctional enzyme siroheme synthase CysG
M. vanbaalenii PYR-1 Mvan_2305 - 0.0 81.36% (397) uroporphyrin-III C-methyltransferase

CLUSTAL 2.0.9 multiple sequence alignment


Rv2847c|M.tuberculosis_H37Rv        --MTENPYLVGLRLAGKKVVVVGGGT----VAQRRLPLLIASGADVHVIA
Mb2872c|M.bovis_AF2122/97           --MTENPYLVGLRLAGKKVVVVGGGT----VAQRRLPLLIASGADVHVIA
MMAR_1886|M.marinum_M               --MTESPYLVGLRLTGRKVVVVGGGT----VAQRRLPLLIASGADVQVIS
MUL_2114|M.ulcerans_Agy99           --MTESPYLVGLRLTGRKVVVVGGGT----VAQRRLPLLIASGADVQVIS
MAV_3703|M.avium_104                --MTESAYLVGLRLTGKKVVVVGGGT----VAQRRLPLLIASGADVHVIS
MSMEG_2618|M.smegmatis_MC2_155      --MTEDAYLVGLRLSGKKVVVVGGGT----VAQRRLPLLLANGADVHVIT
TH_1981|M.thermoresistible__bu      --VSENAYLAGLLLSGKKVIVVGGGG----VAQRRLPLLIASGADVHVIA
Mflv_4047|M.gilvum_PYR-GCK          MSTYDNAYLVGLRLSGKKVVVVGGGT----VAQRRLPLLVSNGADVHVVT
Mvan_2305|M.vanbaalenii_PYR-1       -MTSENAYLVGLRLAGKKVVVVGGGT----VAQRRLPLLVASGADVHVIT
MAB_3143c|M.abscessus_ATCC_199      --MTHDAYLVGLRLAGRKVVVVGAGS----VAQRRLGLLIASGADVHVIA
MLBr_02420|M.leprae_Br4923          -MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDP
                                              *  *   :: .**.*     :   :   :::..* * : .

Rv2847c|M.tuberculosis_H37Rv        PSVTPAVEAMD-------------QITLSVRDYRDGDLDGAWYAIA----
Mb2872c|M.bovis_AF2122/97           PSVTPAVEAMD-------------QITLSVRDYRDGDLDGAWYAIA----
MMAR_1886|M.marinum_M               RSATRSVEVMD-------------KITLTLREYRDGDLEGAWYAIA----
MUL_2114|M.ulcerans_Agy99           RSATRSVEVMD-------------KITLTLREYRDGDLEGAWYAIA----
MAV_3703|M.avium_104                RSATRSVEAMT-------------GITLQLREYRDGDLDGAWYAIA----
MSMEG_2618|M.smegmatis_MC2_155      RAATPVVEALQKDDP---------GITLELRDFRPGDLEGAWYVIA----
TH_1981|M.thermoresistible__bu      RSATPAVEALSRQQP---------GITLELREFRDGDLEGAWYVIA----
Mflv_4047|M.gilvum_PYR-GCK          KAATPAVEAID-------------GITLTLREFRDEDLDGAWYVIA----
Mvan_2305|M.vanbaalenii_PYR-1       RAATPAVEAID-------------GITLTMRDFRAGDLEGAWYVIA----
MAB_3143c|M.abscessus_ATCC_199      PHATPAVEGMA-------------SINLQLRPYQDGDLDGAWYAIA----
MLBr_02420|M.leprae_Br4923          DVPEPVLALIGKDLPPVSGPAPAGPAAVTAGRAADGTLEGDHYQAAPMVM
                                          :  :                 :         *:*  *  *    

Rv2847c|M.tuberculosis_H37Rv        --------ATDDARVNVAVVAEAERRRIFCVR----ADIAVEGTAVTPAS
Mb2872c|M.bovis_AF2122/97           --------ATDDARVNVAVVAEAERRRIFCVR----ADIAVEGTAVTPAS
MMAR_1886|M.marinum_M               --------ATDDPAVNAAIVAEADRRQIFCVR----ADIAVEGTAVTPAT
MUL_2114|M.ulcerans_Agy99           --------ATDDPAVNAAIVAEADRRQIFCVR----ADIAVEGTAVTPAT
MAV_3703|M.avium_104                --------ATDDPRVNEAVVAEAERRRIFCVR----ADVAVAGTAVTPAS
MSMEG_2618|M.smegmatis_MC2_155      --------ATDDPVVNAAIAAEADERRIFCVR----ADIAREGSAVTPAT
TH_1981|M.thermoresistible__bu      --------ATDDPDVNASVVAEADRRRIFCVR----ADVAREGSAVTPAT
Mflv_4047|M.gilvum_PYR-GCK          --------ATDDPAVNAAIVAGAERRRIFCVR----ADIAVEGTAVTPAT
Mvan_2305|M.vanbaalenii_PYR-1       --------ATDDPEVNAAIVAEAESRRVFCVR----ADVAVEGTAVTPAT
MAB_3143c|M.abscessus_ATCC_199      --------CTDDPAVNAAVVDEAERRHIFCVR----ADSARDGTAVTPAS
MLBr_02420|M.leprae_Br4923          SGGADIRPALGDPTEVAKTLTAEARSGVDVVRLVAGDPLTVDAVIAEVNA
                                            . .*.              :  **       :  .  .   :

Rv2847c|M.tuberculosis_H37Rv        FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
Mb2872c|M.bovis_AF2122/97           FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
MMAR_1886|M.marinum_M               FNHAGLSVGVLAS---------------GDHRRSAAIRSAIREALQNGLV
MUL_2114|M.ulcerans_Agy99           FNHAGLSVGVLAS---------------GDHRRSAAIRSAIREALQNGLV
MAV_3703|M.avium_104                FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQTGVI
MSMEG_2618|M.smegmatis_MC2_155      FEHDGLSVGVLAG---------------GEHRRSAAIRSAIHEALQQGLV
TH_1981|M.thermoresistible__bu      FEYEGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
Mflv_4047|M.gilvum_PYR-GCK          FEYEGLEVGVLAG---------------GEHRRSAAIRTAIREALQQGVV
Mvan_2305|M.vanbaalenii_PYR-1       FEYEGLSVGVLAG---------------GEHRRSAGIRTAIREALQRGLI
MAB_3143c|M.abscessus_ATCC_199      FNHDGIAVGVLTG---------------GQHKRSAALRSAIHEALQRGLI
MLBr_02420|M.leprae_Br4923          VARTNLQIEIVPGLVTSSVVPTYAGLPLGSSHTVADVRGDVDWEALAAAP
                                    .   .: : ::..               *. :  * :*  :      .  

Rv2847c|M.tuberculosis_H37Rv        TAQ-----SSDVLSGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
Mb2872c|M.bovis_AF2122/97           TAQ-----SSDVLSGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
MMAR_1886|M.marinum_M               TSTSYQ--SSDVIRGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
MUL_2114|M.ulcerans_Agy99           TSTSYQ--SSDVIRGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
MAV_3703|M.avium_104                TPEGPV--GSDAVKGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
MSMEG_2618|M.smegmatis_MC2_155      TED-----MPGELTSGVALVGGGPGDPE--LITVRGRRLLARADVVVADR
TH_1981|M.thermoresistible__bu      TAEAGADPMSGAIPGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
Mflv_4047|M.gilvum_PYR-GCK          TDESGD--APGVVKGGVALVGGGPGDPE--LITVRGRRLLAHADVVVADR
Mvan_2305|M.vanbaalenii_PYR-1       GVDSSA--APGVAKGTVALVGGGPGDPE--LVTVRGRRLLAHADVVVADR
MAB_3143c|M.abscessus_ATCC_199      TET------ADVKPEGVALVGGGPGDPD--LITVRGRRLLAQADVVVADR
MLBr_02420|M.leprae_Br4923          GPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQRSVETTLEG
                                             .        *:. * .*.   ::*.:*     ::  .. : 

Rv2847c|M.tuberculosis_H37Rv        LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------AVLIE
Mb2872c|M.bovis_AF2122/97           LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------AVLIE
MMAR_1886|M.marinum_M               LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
MUL_2114|M.ulcerans_Agy99           LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
MAV_3703|M.avium_104                LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
MSMEG_2618|M.smegmatis_MC2_155      LAPQELLAELGPHVEVIDAAKIPYGRAMAQEAIN-----------AVLIE
TH_1981|M.thermoresistible__bu      LAPQELLAELPPHVEVIDAAKIPYGRAMAQEAIN-----------ATLID
Mflv_4047|M.gilvum_PYR-GCK          LAPQELLAELSPEVEVIDASKIPYGRAMAQDAIN-----------AVLVD
Mvan_2305|M.vanbaalenii_PYR-1       LAPQDLLSELSPEVEVIDAAKIPYGRAMAQDAIN-----------EVLIA
MAB_3143c|M.abscessus_ATCC_199      LAPAELLADLGPHVEVIDAAKIPYGRAMAQQEIN-----------RVLIE
MLBr_02420|M.leprae_Br4923          LADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVP
                                    **   :*    .   : ..  :. *::::.                .:: 

Rv2847c|M.tuberculosis_H37Rv        RAR--SGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTS----
Mb2872c|M.bovis_AF2122/97           RAR--SGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTS----
MMAR_1886|M.marinum_M               RAK--AGRFVVRLKGGDPFVFARGYEEVLACTEAGIPVIVVPGVTS----
MUL_2114|M.ulcerans_Agy99           RAK--AGRFVVRLKGGDPFVFARGYEEVLACTEAGIPVIVVPGVTS----
MAV_3703|M.avium_104                RAR--AGSFVVRLKGGDPFVFARGYEEVLACADAGIPVTVVPGVTS----
MSMEG_2618|M.smegmatis_MC2_155      RAR--AGKFVVRLKGGDPFVFARGYEEVLACSEAGIPVTVVPGVTS----
TH_1981|M.thermoresistible__bu      RAR--AGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGITS----
Mflv_4047|M.gilvum_PYR-GCK          RAK--AGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGVTS----
Mvan_2305|M.vanbaalenii_PYR-1       RAR--EGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGVTS----
MAB_3143c|M.abscessus_ATCC_199      RAQ--EGKFVVRLKGGDPFVFARGYEEVLACAEAGVQVTVVPGVTS----
MLBr_02420|M.leprae_Br4923          RTKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQ
                                    *::   * :  **.. ..: .      * .   ..    .* *:..    

Rv2847c|M.tuberculosis_H37Rv        --------------------AIAVPAMAGVPVTHR-----------AMTH
Mb2872c|M.bovis_AF2122/97           --------------------AIAVPAMAGVPVTHR-----------AMTH
MMAR_1886|M.marinum_M               --------------------AIAVPALAGVPVTHR-----------ATNH
MUL_2114|M.ulcerans_Agy99           --------------------AIAVPALAGVPVTHR-----------ATNH
MAV_3703|M.avium_104                --------------------AIGVPALAGVPVTHR-----------AVNH
MSMEG_2618|M.smegmatis_MC2_155      --------------------AIAVPALAGVPVTHR-----------GMTH
TH_1981|M.thermoresistible__bu      --------------------AIGVPALAGVPVTHR-----------AVNH
Mflv_4047|M.gilvum_PYR-GCK          --------------------AIAVPALAGVPVTHR-----------GLTH
Mvan_2305|M.vanbaalenii_PYR-1       --------------------AIAVPALAGVPVTHR-----------GLTH
MAB_3143c|M.abscessus_ATCC_199      --------------------AISVPAAAGVPVTHR-----------AVNH
MLBr_02420|M.leprae_Br4923          WVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISP
                                                        .: . * :** ::             . . 

Rv2847c|M.tuberculosis_H37Rv        EFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGG
Mb2872c|M.bovis_AF2122/97           EFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGG
MMAR_1886|M.marinum_M               EFVVVSGHIAPDHPESLVNWNALAAMSGTIVLLMAVERIELFVEALLKGG
MUL_2114|M.ulcerans_Agy99           EFVVVSGHIAPDHPESLVNWNALAAMSGTIVLLMAVERIELFVEALLKGG
MAV_3703|M.avium_104                EFVVVSGHLPPGHPESLVNWDALAALTGTIVLLMAVERIELFAEALLKGG
MSMEG_2618|M.smegmatis_MC2_155      EFVVVSGHVAPGHPESLVNWDALAAMRGTIVLLMAVERIEQFAKVLLEGG
TH_1981|M.thermoresistible__bu      EFVVVSGHVAPGEPESLTNWDALAKLSGTIVLLMAVERIEVFAKALLEGG
Mflv_4047|M.gilvum_PYR-GCK          EFVVVSGHLAPDHPESLVNWDALAAMSGTIVLLMAVERIELFVKVLLKGG
Mvan_2305|M.vanbaalenii_PYR-1       EFVVVSGHVAPDHPESLVNWDALAAMNGTIVLLMAVERIELFVKILLRGG
MAB_3143c|M.abscessus_ATCC_199      EFVVVSGHVAPGHPESLVNWDALAALNGTIVLLMAVERIEQFARVLIEGG
MLBr_02420|M.leprae_Br4923          ELVPAGEQSSLGLLDEFPPYDSVFDPVNRVLLPRADIATETLAEGLRGCG
                                    *:* .. : . .  :.:  ::::    . ::*  *    * :.  *   *

Rv2847c|M.tuberculosis_H37Rv        ------------RTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIR
Mb2872c|M.bovis_AF2122/97           ------------RTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIR
MMAR_1886|M.marinum_M               ------------RPADTPVLVVQHGTTPAQHTLRATLADTPEKVRAEGIR
MUL_2114|M.ulcerans_Agy99           ------------RPAYTPVLVVQHGTTPAQHTLRATLADTPEKVRAEGIR
MAV_3703|M.avium_104                ------------RPADTPVLVVQHGTTAAQHTLRATLADTPEKIRAEGIR
MSMEG_2618|M.smegmatis_MC2_155      ------------RPADTPVLVVQHGTTMAQRTLHATLADAPERIRSEGIR
TH_1981|M.thermoresistible__bu      ------------RPADTPVLVVQHGTTAAQRTLRATLRDAPERMRAEGIR
Mflv_4047|M.gilvum_PYR-GCK          ------------RPADTPVLVVQHGTTSSQRTLRAGLSEVPERIRSDGIR
Mvan_2305|M.vanbaalenii_PYR-1       ------------RPADTPVLVVQHGTTSSQRTLRATLSEAPERIRSDGIR
MAB_3143c|M.abscessus_ATCC_199      ------------RPAHTPVLVVQHGTTDEQRVLRADLATAPERIRSQGIR
MLBr_02420|M.leprae_Br4923          WEIEDVTAYRTVRAAPPPAATREMIKTGGFDAVCFTSSSTVRNLVGIAGK
                                                *.* .*. . :  .*    .:      . ..: . . :

Rv2847c|M.tuberculosis_H37Rv        PPAIIVIGAVVGLSGVRGLNNS----------------------------
Mb2872c|M.bovis_AF2122/97           PPAIIVIGAVVGLSGVRGLNNS----------------------------
MMAR_1886|M.marinum_M               PPAIIVIGAVAAFG-V----------------------------------
MUL_2114|M.ulcerans_Agy99           PPAIIVIGAVATFG-V----------------------------------
MAV_3703|M.avium_104                PPAIIVIGAVAAFG-V----------------------------------
MSMEG_2618|M.smegmatis_MC2_155      PPAIIVIGPVAGFAG-----------------------------------
TH_1981|M.thermoresistible__bu      PPAIIVIGAVAGFDGLKNS-------------------------------
Mflv_4047|M.gilvum_PYR-GCK          PPAIVVIGAVAGFGT-----------------------------------
Mvan_2305|M.vanbaalenii_PYR-1       PPAIIVIGAVAGFGA-----------------------------------
MAB_3143c|M.abscessus_ATCC_199      PPAIIVIGPVAAYGAF----------------------------------
MLBr_02420|M.leprae_Br4923          PHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDALAEHVARLRA
                                    * *  **. :                                        

Rv2847c|M.tuberculosis_H37Rv        --------------
Mb2872c|M.bovis_AF2122/97           --------------
MMAR_1886|M.marinum_M               --------------
MUL_2114|M.ulcerans_Agy99           --------------
MAV_3703|M.avium_104                --------------
MSMEG_2618|M.smegmatis_MC2_155      --------------
TH_1981|M.thermoresistible__bu      --------------
Mflv_4047|M.gilvum_PYR-GCK          --------------
Mvan_2305|M.vanbaalenii_PYR-1       --------------
MAB_3143c|M.abscessus_ATCC_199      --------------
MLBr_02420|M.leprae_Br4923          EGALPPPRKKSRRR

species	id	gene	e-value	identity (len)	annotation
M. tuberculosis H37Rv	Rv2847c	cysG	-	100% (405)	POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN-III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT) (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2 OXIDASE + FERROCHELATASE
M. tuberculosis H37Rv	Rv2071c	cobM	2e-24	34.16% (243)	precorrin-4 C11-methyltransferase CobM
M. tuberculosis H37Rv	Rv0511	hemD	5e-19	29.50% (261)	uroporphyrin-III C-methyltransferase HemD