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M. marinum M MMAR_1886 (cysG)

annotation: multifunctional enzyme siroheme synthase CysG
coordinates: 2298950 - 2300155
length: 401

VTESPYLVGLRLTGRKVVVVGGGTVAQRRLPLLIASGADVQVISRSATRSVEVMDKITLTLREYRDGDLE
GAWYAIAATDDPAVNAAIVAEADRRQIFCVRADIAVEGTAVTPATFNHAGLSVGVLASGDHRRSAAIRSA
IREALQNGLVTSTSYQSSDVIRGGVALIGGGPGDPELITVRGRRLLAQADVVVADRLAPPELLAELPPHV
EVIDAAKIPYGRAMAQDAINDVMIERAKAGRFVVRLKGGDPFVFARGYEEVLACTEAGIPVIVVPGVTSA
IAVPALAGVPVTHRATNHEFVVVSGHIAPDHPESLVNWNALAAMSGTIVLLMAVERIELFVEALLKGGRP
ADTPVLVVQHGTTPAQHTLRATLADTPEKVRAEGIRPPAIIVIGAVAAFGV

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. marinum M MMAR_1886 cysG - 100% (401) multifunctional enzyme siroheme synthase CysG
M. marinum M MMAR_3053 cobM 2e-22 34.58% (240) precorrin-4 C11-methyltransferase, CobM
M. marinum M MMAR_0844 hemD 1e-17 27.31% (271) uroporphyrin-III C-methyltransferase HemD

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb2872c cysG 0.0 85.35% (396) multifunctional uroporphyrinogen III methylase/precorrin-2
M. gilvum PYR-GCK Mflv_4047 - 0.0 80.65% (398) uroporphyrin-III C-methyltransferase
M. tuberculosis H37Rv Rv2847c cysG 0.0 85.35% (396) multifunctional uroporphyrinogen III methylase/precorrin-2
M. leprae Br4923 MLBr_02420 hemD 1e-16 27.11% (273) putative uroporphyrin-III C-methyltransferase
M. abscessus ATCC 19977 MAB_3143c - 1e-170 76.50% (400) multifunctional siroheme synthase CysG/uroporphyrin-III
M. avium 104 MAV_3703 cobA 0.0 88.03% (401) uroporphyrin-III C-methyltransferase
M. smegmatis MC2 155 MSMEG_2618 cobA 1e-178 80.40% (403) uroporphyrin-III C-methyltransferase
M. thermoresistible (build 8) TH_1981 cysG2 1e-180 79.51% (405) POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG:
M. ulcerans Agy99 MUL_2114 cysG 0.0 99.50% (401) multifunctional enzyme siroheme synthase CysG
M. vanbaalenii PYR-1 Mvan_2305 - 0.0 79.45% (399) uroporphyrin-III C-methyltransferase

CLUSTAL 2.0.9 multiple sequence alignment


MMAR_1886|M.marinum_M               --MTESPYLVGLRLTGRKVVVVGGGT----VAQRRLPLLIASGADVQVIS
MUL_2114|M.ulcerans_Agy99           --MTESPYLVGLRLTGRKVVVVGGGT----VAQRRLPLLIASGADVQVIS
MAV_3703|M.avium_104                --MTESAYLVGLRLTGKKVVVVGGGT----VAQRRLPLLIASGADVHVIS
Mb2872c|M.bovis_AF2122/97           --MTENPYLVGLRLAGKKVVVVGGGT----VAQRRLPLLIASGADVHVIA
Rv2847c|M.tuberculosis_H37Rv        --MTENPYLVGLRLAGKKVVVVGGGT----VAQRRLPLLIASGADVHVIA
MSMEG_2618|M.smegmatis_MC2_155      --MTEDAYLVGLRLSGKKVVVVGGGT----VAQRRLPLLLANGADVHVIT
TH_1981|M.thermoresistible__bu      --VSENAYLAGLLLSGKKVIVVGGGG----VAQRRLPLLIASGADVHVIA
Mflv_4047|M.gilvum_PYR-GCK          MSTYDNAYLVGLRLSGKKVVVVGGGT----VAQRRLPLLVSNGADVHVVT
Mvan_2305|M.vanbaalenii_PYR-1       -MTSENAYLVGLRLAGKKVVVVGGGT----VAQRRLPLLVASGADVHVIT
MAB_3143c|M.abscessus_ATCC_199      --MTHDAYLVGLRLAGRKVVVVGAGS----VAQRRLGLLIASGADVHVIA
MLBr_02420|M.leprae_Br4923          -MVGATMTTRGRKLRPGRITFVGAGPGAPGLLTAQAVAVLANAALVFIDP
                                              *  *   :: .**.*     :   :   :::..* * : .

MMAR_1886|M.marinum_M               RSATRSVEVMD-------------KITLTLREYRDGDLEGAWYAIA----
MUL_2114|M.ulcerans_Agy99           RSATRSVEVMD-------------KITLTLREYRDGDLEGAWYAIA----
MAV_3703|M.avium_104                RSATRSVEAMT-------------GITLQLREYRDGDLDGAWYAIA----
Mb2872c|M.bovis_AF2122/97           PSVTPAVEAMD-------------QITLSVRDYRDGDLDGAWYAIA----
Rv2847c|M.tuberculosis_H37Rv        PSVTPAVEAMD-------------QITLSVRDYRDGDLDGAWYAIA----
MSMEG_2618|M.smegmatis_MC2_155      RAATPVVEALQKDDP---------GITLELRDFRPGDLEGAWYVIA----
TH_1981|M.thermoresistible__bu      RSATPAVEALSRQQP---------GITLELREFRDGDLEGAWYVIA----
Mflv_4047|M.gilvum_PYR-GCK          KAATPAVEAID-------------GITLTLREFRDEDLDGAWYVIA----
Mvan_2305|M.vanbaalenii_PYR-1       RAATPAVEAID-------------GITLTMRDFRAGDLEGAWYVIA----
MAB_3143c|M.abscessus_ATCC_199      PHATPAVEGMA-------------SINLQLRPYQDGDLDGAWYAIA----
MLBr_02420|M.leprae_Br4923          DVPEPVLALIGKDLPPVSGPAPAGPAAVTAGRAADGTLEGDHYQAAPMVM
                                          :  :                 :         *:*  *  *    

MMAR_1886|M.marinum_M               --------ATDDPAVNAAIVAEADRRQIFCVR----ADIAVEGTAVTPAT
MUL_2114|M.ulcerans_Agy99           --------ATDDPAVNAAIVAEADRRQIFCVR----ADIAVEGTAVTPAT
MAV_3703|M.avium_104                --------ATDDPRVNEAVVAEAERRRIFCVR----ADVAVAGTAVTPAS
Mb2872c|M.bovis_AF2122/97           --------ATDDARVNVAVVAEAERRRIFCVR----ADIAVEGTAVTPAS
Rv2847c|M.tuberculosis_H37Rv        --------ATDDARVNVAVVAEAERRRIFCVR----ADIAVEGTAVTPAS
MSMEG_2618|M.smegmatis_MC2_155      --------ATDDPVVNAAIAAEADERRIFCVR----ADIAREGSAVTPAT
TH_1981|M.thermoresistible__bu      --------ATDDPDVNASVVAEADRRRIFCVR----ADVAREGSAVTPAT
Mflv_4047|M.gilvum_PYR-GCK          --------ATDDPAVNAAIVAGAERRRIFCVR----ADIAVEGTAVTPAT
Mvan_2305|M.vanbaalenii_PYR-1       --------ATDDPEVNAAIVAEAESRRVFCVR----ADVAVEGTAVTPAT
MAB_3143c|M.abscessus_ATCC_199      --------CTDDPAVNAAVVDEAERRHIFCVR----ADSARDGTAVTPAS
MLBr_02420|M.leprae_Br4923          SGGADIRPALGDPTEVAKTLTAEARSGVDVVRLVAGDPLTVDAVIAEVNA
                                            . .*.              :  **       :  .  .   :

MMAR_1886|M.marinum_M               FNHAGLSVGVLAS---------------GDHRRSAAIRSAIREALQNGLV
MUL_2114|M.ulcerans_Agy99           FNHAGLSVGVLAS---------------GDHRRSAAIRSAIREALQNGLV
MAV_3703|M.avium_104                FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQTGVI
Mb2872c|M.bovis_AF2122/97           FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
Rv2847c|M.tuberculosis_H37Rv        FSYAGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
MSMEG_2618|M.smegmatis_MC2_155      FEHDGLSVGVLAG---------------GEHRRSAAIRSAIHEALQQGLV
TH_1981|M.thermoresistible__bu      FEYEGLSVGVLAG---------------GEHRRSAAIRSAIREALQQGVI
Mflv_4047|M.gilvum_PYR-GCK          FEYEGLEVGVLAG---------------GEHRRSAAIRTAIREALQQGVV
Mvan_2305|M.vanbaalenii_PYR-1       FEYEGLSVGVLAG---------------GEHRRSAGIRTAIREALQRGLI
MAB_3143c|M.abscessus_ATCC_199      FNHDGIAVGVLTG---------------GQHKRSAALRSAIHEALQRGLI
MLBr_02420|M.leprae_Br4923          VARTNLQIEIVPGLVTSSVVPTYAGLPLGSSHTVADVRGDVDWEALAAAP
                                    .   .: : ::..               *. :  * :*  :      .  

MMAR_1886|M.marinum_M               TSTSYQ--SSDVIRGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
MUL_2114|M.ulcerans_Agy99           TSTSYQ--SSDVIRGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
MAV_3703|M.avium_104                TPEGPV--GSDAVKGGVALIGGGPGDPE--LITVRGRRLLAQADVVVADR
Mb2872c|M.bovis_AF2122/97           TAQ-----SSDVLSGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
Rv2847c|M.tuberculosis_H37Rv        TAQ-----SSDVLSGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
MSMEG_2618|M.smegmatis_MC2_155      TED-----MPGELTSGVALVGGGPGDPE--LITVRGRRLLARADVVVADR
TH_1981|M.thermoresistible__bu      TAEAGADPMSGAIPGGVALVGGGPGDPE--LITVRGRRLLAQADVVVADR
Mflv_4047|M.gilvum_PYR-GCK          TDESGD--APGVVKGGVALVGGGPGDPE--LITVRGRRLLAHADVVVADR
Mvan_2305|M.vanbaalenii_PYR-1       GVDSSA--APGVAKGTVALVGGGPGDPE--LVTVRGRRLLAHADVVVADR
MAB_3143c|M.abscessus_ATCC_199      TET------ADVKPEGVALVGGGPGDPD--LITVRGRRLLAQADVVVADR
MLBr_02420|M.leprae_Br4923          GPLILQATASHLADAAHILIDHGLDDATPCVVTAQGTTCQQRSVETTLEG
                                             .        *:. * .*.   ::*.:*     ::  .. : 

MMAR_1886|M.marinum_M               LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
MUL_2114|M.ulcerans_Agy99           LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
MAV_3703|M.avium_104                LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------DVMIE
Mb2872c|M.bovis_AF2122/97           LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------AVLIE
Rv2847c|M.tuberculosis_H37Rv        LAPPELLAELPPHVEVIDAAKIPYGRAMAQDAIN-----------AVLIE
MSMEG_2618|M.smegmatis_MC2_155      LAPQELLAELGPHVEVIDAAKIPYGRAMAQEAIN-----------AVLIE
TH_1981|M.thermoresistible__bu      LAPQELLAELPPHVEVIDAAKIPYGRAMAQEAIN-----------ATLID
Mflv_4047|M.gilvum_PYR-GCK          LAPQELLAELSPEVEVIDASKIPYGRAMAQDAIN-----------AVLVD
Mvan_2305|M.vanbaalenii_PYR-1       LAPQDLLSELSPEVEVIDAAKIPYGRAMAQDAIN-----------EVLIA
MAB_3143c|M.abscessus_ATCC_199      LAPAELLADLGPHVEVIDAAKIPYGRAMAQQEIN-----------RVLIE
MLBr_02420|M.leprae_Br4923          LADSAVLVGTDTAGPLTGPLVVTIGKTVTSRARLNWWESRALYGWTVLVP
                                    **   :*    .   : ..  :. *::::.                .:: 

MMAR_1886|M.marinum_M               RAK--AGRFVVRLKGGDPFVFARGYEEVLACTEAGIPVIVVPGVTS----
MUL_2114|M.ulcerans_Agy99           RAK--AGRFVVRLKGGDPFVFARGYEEVLACTEAGIPVIVVPGVTS----
MAV_3703|M.avium_104                RAR--AGSFVVRLKGGDPFVFARGYEEVLACADAGIPVTVVPGVTS----
Mb2872c|M.bovis_AF2122/97           RAR--SGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTS----
Rv2847c|M.tuberculosis_H37Rv        RAR--SGNFVVRLKGGDPFVFARGYEEVLACAHAGIPVTVVPGVTS----
MSMEG_2618|M.smegmatis_MC2_155      RAR--AGKFVVRLKGGDPFVFARGYEEVLACSEAGIPVTVVPGVTS----
TH_1981|M.thermoresistible__bu      RAR--AGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGITS----
Mflv_4047|M.gilvum_PYR-GCK          RAK--AGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGVTS----
Mvan_2305|M.vanbaalenii_PYR-1       RAR--EGKFVVRLKGGDPFVFARGYEEVIACAEAGIPVTVVPGVTS----
MAB_3143c|M.abscessus_ATCC_199      RAQ--EGKFVVRLKGGDPFVFARGYEEVLACAEAGVQVTVVPGVTS----
MLBr_02420|M.leprae_Br4923          RTKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQ
                                    *::   * :  **.. ..: .      * .   ..    .* *:..    

MMAR_1886|M.marinum_M               --------------------AIAVPALAGVPVTHR-----------ATNH
MUL_2114|M.ulcerans_Agy99           --------------------AIAVPALAGVPVTHR-----------ATNH
MAV_3703|M.avium_104                --------------------AIGVPALAGVPVTHR-----------AVNH
Mb2872c|M.bovis_AF2122/97           --------------------AIAVPAMAGVPVTHR-----------AMTH
Rv2847c|M.tuberculosis_H37Rv        --------------------AIAVPAMAGVPVTHR-----------AMTH
MSMEG_2618|M.smegmatis_MC2_155      --------------------AIAVPALAGVPVTHR-----------GMTH
TH_1981|M.thermoresistible__bu      --------------------AIGVPALAGVPVTHR-----------AVNH
Mflv_4047|M.gilvum_PYR-GCK          --------------------AIAVPALAGVPVTHR-----------GLTH
Mvan_2305|M.vanbaalenii_PYR-1       --------------------AIAVPALAGVPVTHR-----------GLTH
MAB_3143c|M.abscessus_ATCC_199      --------------------AISVPAAAGVPVTHR-----------AVNH
MLBr_02420|M.leprae_Br4923          WVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGEATADRVRAFGISP
                                                        .: . * :** ::             . . 

MMAR_1886|M.marinum_M               EFVVVSGHIAPDHPESLVNWNALAAMSGTIVLLMAVERIELFVEALLKGG
MUL_2114|M.ulcerans_Agy99           EFVVVSGHIAPDHPESLVNWNALAAMSGTIVLLMAVERIELFVEALLKGG
MAV_3703|M.avium_104                EFVVVSGHLPPGHPESLVNWDALAALTGTIVLLMAVERIELFAEALLKGG
Mb2872c|M.bovis_AF2122/97           EFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGG
Rv2847c|M.tuberculosis_H37Rv        EFVVVSGHLAPGHPESLVNWDALAALTGTIVLLMAVERIELFVDVLLKGG
MSMEG_2618|M.smegmatis_MC2_155      EFVVVSGHVAPGHPESLVNWDALAAMRGTIVLLMAVERIEQFAKVLLEGG
TH_1981|M.thermoresistible__bu      EFVVVSGHVAPGEPESLTNWDALAKLSGTIVLLMAVERIEVFAKALLEGG
Mflv_4047|M.gilvum_PYR-GCK          EFVVVSGHLAPDHPESLVNWDALAAMSGTIVLLMAVERIELFVKVLLKGG
Mvan_2305|M.vanbaalenii_PYR-1       EFVVVSGHVAPDHPESLVNWDALAAMNGTIVLLMAVERIELFVKILLRGG
MAB_3143c|M.abscessus_ATCC_199      EFVVVSGHVAPGHPESLVNWDALAALNGTIVLLMAVERIEQFARVLIEGG
MLBr_02420|M.leprae_Br4923          ELVPAGEQSSLGLLDEFPPYDSVFDPVNRVLLPRADIATETLAEGLRGCG
                                    *:* .. : . .  :.:  ::::    . ::*  *    * :.  *   *

MMAR_1886|M.marinum_M               ------------RPADTPVLVVQHGTTPAQHTLRATLADTPEKVRAEGIR
MUL_2114|M.ulcerans_Agy99           ------------RPAYTPVLVVQHGTTPAQHTLRATLADTPEKVRAEGIR
MAV_3703|M.avium_104                ------------RPADTPVLVVQHGTTAAQHTLRATLADTPEKIRAEGIR
Mb2872c|M.bovis_AF2122/97           ------------RTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIR
Rv2847c|M.tuberculosis_H37Rv        ------------RTADTPVLVVQHGTTAAQQTLRATLADTPEKVRAAGIR
MSMEG_2618|M.smegmatis_MC2_155      ------------RPADTPVLVVQHGTTMAQRTLHATLADAPERIRSEGIR
TH_1981|M.thermoresistible__bu      ------------RPADTPVLVVQHGTTAAQRTLRATLRDAPERMRAEGIR
Mflv_4047|M.gilvum_PYR-GCK          ------------RPADTPVLVVQHGTTSSQRTLRAGLSEVPERIRSDGIR
Mvan_2305|M.vanbaalenii_PYR-1       ------------RPADTPVLVVQHGTTSSQRTLRATLSEAPERIRSDGIR
MAB_3143c|M.abscessus_ATCC_199      ------------RPAHTPVLVVQHGTTDEQRVLRADLATAPERIRSQGIR
MLBr_02420|M.leprae_Br4923          WEIEDVTAYRTVRAAPPPAATREMIKTGGFDAVCFTSSSTVRNLVGIAGK
                                                *.* .*. . :  .*    .:      . ..: . . :

MMAR_1886|M.marinum_M               PPAIIVIGAVAAFG-V----------------------------------
MUL_2114|M.ulcerans_Agy99           PPAIIVIGAVATFG-V----------------------------------
MAV_3703|M.avium_104                PPAIIVIGAVAAFG-V----------------------------------
Mb2872c|M.bovis_AF2122/97           PPAIIVIGAVVGLSGVRGLNNS----------------------------
Rv2847c|M.tuberculosis_H37Rv        PPAIIVIGAVVGLSGVRGLNNS----------------------------
MSMEG_2618|M.smegmatis_MC2_155      PPAIIVIGPVAGFAG-----------------------------------
TH_1981|M.thermoresistible__bu      PPAIIVIGAVAGFDGLKNS-------------------------------
Mflv_4047|M.gilvum_PYR-GCK          PPAIVVIGAVAGFGT-----------------------------------
Mvan_2305|M.vanbaalenii_PYR-1       PPAIIVIGAVAGFGA-----------------------------------
MAB_3143c|M.abscessus_ATCC_199      PPAIIVIGPVAAYGAF----------------------------------
MLBr_02420|M.leprae_Br4923          PHARTVIACIGPKTAETASEFGLRVDVQPETAAVGPLVDALAEHVARLRA
                                    * *  **. :                                        

MMAR_1886|M.marinum_M               --------------
MUL_2114|M.ulcerans_Agy99           --------------
MAV_3703|M.avium_104                --------------
Mb2872c|M.bovis_AF2122/97           --------------
Rv2847c|M.tuberculosis_H37Rv        --------------
MSMEG_2618|M.smegmatis_MC2_155      --------------
TH_1981|M.thermoresistible__bu      --------------
Mflv_4047|M.gilvum_PYR-GCK          --------------
Mvan_2305|M.vanbaalenii_PYR-1       --------------
MAB_3143c|M.abscessus_ATCC_199      --------------
MLBr_02420|M.leprae_Br4923          EGALPPPRKKSRRR

species	id	gene	e-value	identity (len)	annotation
M. marinum M	MMAR_1886	cysG	-	100% (401)	multifunctional enzyme siroheme synthase CysG
M. marinum M	MMAR_3053	cobM	2e-22	34.58% (240)	precorrin-4 C11-methyltransferase, CobM
M. marinum M	MMAR_0844	hemD	1e-17	27.31% (271)	uroporphyrin-III C-methyltransferase HemD