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TAAGDAATHVTDVLVIGGGPAATWAAIAAAESGSRVTLVDKGYCGTSGATAAGGNNLWLIPPGPRRDESI REREAAAGGLTDAEWMARVLSASWDRIEQLADWGYPFPTGDDGRQLRVSLQGPEYMRRMRRKAHRSGVRI LDHHPATALTTDSDGIVNGATGIARQDAGRPWRIGAGAVVLATGGTAFLSGSLGTNVDTGDGYLMAAEAG AALSGMEFSTAYALAPEWGTHTKGRMLAWASFYDEDGHPIATECGLAGRRDAQRALARGQRVFARLDRAP EHIRRVMRDAQPNYFLPLDKAGIDPFVTAYPLRMVYEGSVRGTGGLRLVGPDCATSVPGLYAAGDAATRE LITGAISGGGSHNGSWAIASGSFAGRGAAEYSRRRTFAGFVEVDGVGLRPGPQHAPTVDDVVRVAQDHIL PPMRSHLKSQVRLTESAGVLEQLWTDIAAGLAPVPARDAYKPRQAVALVAAARWITAASLARAETRGIHR REDLPGTDHRLSHRILVGGLDTVWTIPDPVLPELISSEAVA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_3157 | - | - | 100% (532) | fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0571 | - | 6e-21 | 24.45% (544) | fumarate reductase/succinate dehydrogenase flavoprotein |
| M. gilvum PYR-GCK | Mflv_3618 | - | 2e-20 | 27.00% (537) | L-aspartate oxidase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1578 | frdA | 2e-18 | 25.71% (560) | fumarate reductase flavoprotein subunit |
| M. tuberculosis H37Rv | Rv1552 | frdA | 2e-18 | 25.71% (560) | fumarate reductase flavoprotein subunit |
| M. leprae Br4923 | MLBr_01226 | nadB | 2e-18 | 27.24% (536) | L-aspartate oxidase |
| M. abscessus ATCC 19977 | MAB_2673c | - | 4e-20 | 27.43% (525) | L-aspartate oxidase |
| M. marinum M | MMAR_2392 | nadB | 8e-17 | 27.00% (537) | l-aspartate oxidase NadB |
| M. avium 104 | MAV_3190 | nadB | 3e-19 | 28.28% (541) | L-aspartate oxidase |
| M. smegmatis MC2 155 | MSMEG_5532 | - | 0.0 | 76.68% (536) | FAD dependent oxidoreductase |
| M. thermoresistible (build 8) | TH_0808 | nadB | 6e-13 | 27.83% (406) | L-aspartate oxidase |
| M. ulcerans Agy99 | MUL_1569 | nadB | 6e-18 | 27.19% (537) | L-aspartate oxidase |
| M. vanbaalenii PYR-1 | Mvan_0484 | - | 7e-21 | 24.45% (544) | fumarate reductase/succinate dehydrogenase flavoprotein |
CLUSTAL 2.0.9 multiple sequence alignment
MLBr_01226|M.leprae_Br4923 MMAVP--------DWKHAADVVVIGTGVAGLAAALAAHRAGRNVVVLTKA
MAV_3190|M.avium_104 MIARP--------QWTHSADVVVIGTGVAGLAAALAAHRADRSVVVLSKA
MMAR_2392|M.marinum_M MRTSP--------TWRDAADVVVIGTGVAGLAAALSAHRAGRSVVLLNKA
MUL_1569|M.ulcerans_Agy99 MRTSP--------TWRDAADVVVIGTGVAGLAAALSAHRAGRSVVLLNKA
TH_0808|M.thermoresistible__bu MIHRFGCGGSGSVAWQQRADVVVIGTGVAGLVAARAAGRAGRRVVVLSKT
MAB_2673c|M.abscessus_ATCC_199 MTMPAG------TTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLS--
Mb1578|M.bovis_AF2122/97 --------------MTAQHNIVVIGGGGAGLRAAIAIAETNPHLDVAIVS
Rv1552|M.tuberculosis_H37Rv --------------MTAQHNIVVIGGGGAGLRAAIAIAETNPHLDVAIVS
Mflv_3157|M.gilvum_PYR-GCK MTAAGD-------AATHVTDVLVIGGGPAATWAAIAAAESGSRVTLVDKG
MSMEG_5532|M.smegmatis_MC2_155 --MTGR-------PDTLTTDVLVIGGGPAATWAAISATEAGARVILVDKG
Mvan_0484|M.vanbaalenii_PYR-1 MGDLD--------VSVQECDVLVVGGGIGGVRAALRAAELGSSVILVEKA
:::*:* * .. ** . . : :
MLBr_01226|M.leprae_Br4923 DQRRGETATHYAQGGIAVVLPGSDDSVDAHASDTLAAGAGMCNLDTVYSI
MAV_3190|M.avium_104 DQARGATATHYAQGGIAVVLPHNDDSVEAHVADTVVAGAGLCDPQAVYSI
MMAR_2392|M.marinum_M ADTVGATATHYAQGGIAVVLPGSDDSVDAHVADTVTAGAGLCDLEAVYSI
MUL_1569|M.ulcerans_Agy99 TDTVGATATHYAQGGIAVVLPGGDDSVDAHVADTVTAGAGLCDLEAVYSI
TH_0808|M.thermoresistible__bu DD-IGQTATRYAQGGIAVVLPDTDDSVDVHVADTLAAGGGLCDPRIVRSI
MAB_2673c|M.abscessus_ATCC_199 --KVGATATHFAQGGIAVVLPDSQDSIEAHVLDTLSAGAGLCDAEAVRSI
Mb1578|M.bovis_AF2122/97 KVYPMRSHTVSAEGGAAAVTGD-DDSLDEHAHDTVSGGDWLCDQDAVEAF
Rv1552|M.tuberculosis_H37Rv KVYPMRSHTVSAEGGAAAVTGD-DDSLDEHAHDTVSGGDWLCDQDAVEAF
Mflv_3157|M.gilvum_PYR-GCK ---YCGTSGATAAGGNNLWLIPPGPRRDESIREREAAAGGLTDAEWMARV
MSMEG_5532|M.smegmatis_MC2_155 ---YCGTSGATAAGGNNLWLIPPGPRREESVRDREKAAGRITDADWMFRV
Mvan_0484|M.vanbaalenii_PYR-1 VVSRAGPMTYVHS-QYAPDHRVQGEEMTEWAREFVVGSNYLADQDWVQQY
. : .. : : :
MLBr_01226|M.leprae_Br4923 VAEGYHAVTELVGYGARFDESIPGRWAVTCEGGHSRRRIVHAGGDATGAE
MAV_3190|M.avium_104 VADGYAAVSELVGDGARFDESAPGQWALTREGGHSRRRIVHAGGDATGAE
MMAR_2392|M.marinum_M VADGFRAVTDLVGDGAKFDETSTGRWALTREGGHSRRRIVHAGGDATGAE
MUL_1569|M.ulcerans_Agy99 VADGFRAVTDLVGDGAKFDETSTGRWALTREGGHSRRRIVHAGGDATGAE
TH_0808|M.thermoresistible__bu VADGYAAVCDLIGAGARFDRAASGGWARTREGGHTRRRVIHAGGDATGVE
MAB_2673c|M.abscessus_ATCC_199 VSDGYRAVRDLVADGARFDESRSGQWALTREGGHSARRIIHAGGDATGAE
Mb1578|M.bovis_AF2122/97 VAEAPKELVQLEHWGCPWSRKPDGRVAVRPFGGMKKLRTWFAADKTGFHL
Rv1552|M.tuberculosis_H37Rv VAEAPKELVQLEHWGCPWSRKPDGRVAVRPFGGMKKLRTWFAADKTGFHL
Mflv_3157|M.gilvum_PYR-GCK LSASWDRIEQLADWGYPFPTGDDGRQLRVSLQGPEYMRRMRRKAHRSGVR
MSMEG_5532|M.smegmatis_MC2_155 LATAWDRVDQLAAWGYPFPAGSDGRPMRSSLQGPEYMRRMRRKVHRSGVR
Mvan_0484|M.vanbaalenii_PYR-1 IAEAYDRVNEQIEWGVPYSTHEDGSLKYVTVRGHNLGTTLGVDGRVCMEI
:: . : : * : * *
MLBr_01226|M.leprae_Br4923 VQRALDHAADVLDIR--TSHLALRVLHDG----TVVNGVSVLNPNG--WG
MAV_3190|M.avium_104 VQRALDHAARMLDIR--TAHVALRVLHDGSMGDEAVTGVLVGNPHG--YG
MMAR_2392|M.marinum_M VQRALDQAAGLLDIR--ASHVALRILHDD----TAVTGVLVLNPNG--VG
MUL_1569|M.ulcerans_Agy99 VQRALDQAAGLLDIR--ASHVALRILHDD----TAVTGVLVLNPNG--VG
TH_0808|M.thermoresistible__bu VQRALEAAAAGLDIR--CRHQAVQVLVRD----GAVAGVLVRNDDG--LG
MAB_2673c|M.abscessus_ATCC_199 VQRALDHAAAMLDVR--RSHVVHDILVGP----EGVGGVLVGNSDG--IG
Mb1578|M.bovis_AF2122/97 LHTLFQRLLTYSDVMRYDEWFATTLLVDD----GRVCGLVAIELATGRIE
Rv1552|M.tuberculosis_H37Rv LHTLFQRLLTYSDVMRYDEWFATTLLVDD----GRVCGLVAIELATGRIE
Mflv_3157|M.gilvum_PYR-GCK ILDHHPATALTTDSD--------GIVNG-------ATGIARQDAGR--PW
MSMEG_5532|M.smegmatis_MC2_155 ILDHHPALELTVDDD--------GVVDG-------ATGIARQEGGR--PW
Mvan_0484|M.vanbaalenii_PYR-1 LKERMKAAGVRLFER--VDVIDLLTTDGCRPTAGAVCGAVGVNVVTGQCH
: . * :
MLBr_01226|M.leprae_Br4923 IVSAPSVILASGGLGHLYGATTNPEGSTGDGIALALWAGVAVSDLEFIQF
MAV_3190|M.avium_104 IISAPSVILASGGLGHLYGATTNPEGSTGDGIALGLWAGVAVSDLEFIQF
MMAR_2392|M.marinum_M IISAPSVILATGGLGQLYSATTNPDGATGDGIALALWAGVTVSDLEFIQF
MUL_1569|M.ulcerans_Agy99 IISAPSVILATGGLGQLYSATTNPDGATGDGIALALWAGVTVSDLEFIQF
TH_0808|M.thermoresistible__bu VLHTPAVVIATGGLGQLYTATTNPDGSTGDGVALALTAGAAVSDLEFIQF
MAB_2673c|M.abscessus_ATCC_199 VIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQF
Mb1578|M.bovis_AF2122/97 TILADAVILCTGGCGRVFPFTTNANIKTGDGMALAFRAGAPLKDMEFVQY
Rv1552|M.tuberculosis_H37Rv TILADAVILCTGGCGRVFPFTTNANIKTGDGMALAFRAGAPLKDMEFVQY
Mflv_3157|M.gilvum_PYR-GCK RIGAGAVVLATGGT-AFLSGSLGTNVDTGDGYLMAAEAGAALSGMEFSTA
MSMEG_5532|M.smegmatis_MC2_155 RVTAGAVVLATGGT-AFLSGTFGTNVDTGDGLLMAAEHGAALSGMEFSTA
Mvan_0484|M.vanbaalenii_PYR-1 VFTAGSVILNTGPWYPKLHYAFADHCS-GEGHVAAWRVGAEFAGMEFGQF
. : :*:: :* : . *:* . *. . .:**
MLBr_01226|M.leprae_Br4923 HPTMLFAAGTGTGGRRPLVTEAIRGEGAILLDSK----------GNSVTS
MAV_3190|M.avium_104 HPTMLFAPGAG-GGRRPLVTEAIRGEGAKLIDSQ----------GDSVTA
MMAR_2392|M.marinum_M HPTMLFDGSSG--GRRPLITEAIRGEGAILLDRQ----------GRSITA
MUL_1569|M.ulcerans_Agy99 HPTMLFYGSSG--GRRPLITEAIRGEGAILLDRQ----------GRSITA
TH_0808|M.thermoresistible__bu HPTMLFDGASG--GRRPLITEAVRGEGATLVDAR----------GEPVMA
MAB_2673c|M.abscessus_ATCC_199 HPTMLHTPDAA--GCRPLISEAVRGEGAVLVDAR----------GDSVTA
Mb1578|M.bovis_AF2122/97 HPTGLP--FTG-----ILITEAARAEGGWLLNKDGYRYLQDYDLGKPTPE
Rv1552|M.tuberculosis_H37Rv HPTGLP--FTG-----ILITEAARAEGGWLLNKDGYRYLQDYDLGKPTPE
Mflv_3157|M.gilvum_PYR-GCK YALAPEWGTHT---------KGRMLAWASFYDED----------GHPIAT
MSMEG_5532|M.smegmatis_MC2_155 YALAPEWGTHT---------KGRMLQWGSFFDEH----------GQPLPT
Mvan_0484|M.vanbaalenii_PYR-1 AAWSYFNRSFF-----TPGQAKIQGIGGKFCNAAG---------EYFMDR
. . : :
MLBr_01226|M.leprae_Br4923 GVHPLGDLAPRDVVAAAIDARLK------ATGDSCVYLDARGIDG--FAS
MAV_3190|M.avium_104 GVHPMGDLAPRDVVAAAIDARLK------ATGDACVYLDARHIFD--FAD
MMAR_2392|M.marinum_M GVHPLGDLAPRDVVAAAIDARMK------ANGDPCAYLDARSIKG--FES
MUL_1569|M.ulcerans_Agy99 GVHPLGDLAPRDVVAAAIDARMK------ANGDPCAYLDARSIKG--FES
TH_0808|M.thermoresistible__bu GIHPMGDLAPRDVVAAAIEARLA------ATGDPCVFLDARRVAD--FPR
MAB_2673c|M.abscessus_ATCC_199 GVHPQGDLAPRDVVAGAIARRLR------ESGDQCVYLDARAIGN--FAI
Mb1578|M.bovis_AF2122/97 PRLRSMELGPRDRLSQAFVHEHNKGRTVDTPYGPVVYLDLRHLGADLIDA
Rv1552|M.tuberculosis_H37Rv PRLRSMELGPRDRLSQAFVHEHNKGRTVDTPYGPVVYLDLRHLGADLIDA
Mflv_3157|M.gilvum_PYR-GCK ECGLAGRRDAQRALARGQRVFAR------------LDRAPEHIRR-VMRD
MSMEG_5532|M.smegmatis_MC2_155 QLGLGGRQDAQAQLAHGRRVFAR------------IDRAPAHIHQ-TMRD
Mvan_0484|M.vanbaalenii_PYR-1 YDPIWGDKGGLFTIARAIMTEMVE-------GRGPCYLDLRHVPQADLEV
:: . : :
MLBr_01226|M.leprae_Br4923 RFPTVTAACRTVG---IDPAREPIPVVPGAHYSCGG----IVTDAYGQTE
MAV_3190|M.avium_104 RFPTVTAACRAAG---IDPVRQPIPVVPGAHYSCGG----VVTDVYGRTE
MMAR_2392|M.marinum_M RFPTVTASCRAAG---IDPVRQPIPVVPGAHYSCGG----VITDVYGNTE
MUL_1569|M.ulcerans_Agy99 RFPTVTASCRAAG---IDPVRQPIPVVPGAHYSCGG----VITDVYGNTE
TH_0808|M.thermoresistible__bu RFPTVTAVCHAAG---CDPVRQPIPVVPGAHYSCGG----IDTDDQGRST
MAB_2673c|M.abscessus_ATCC_199 RFPTITAACAAAG---IDPSVQLIPVVPGAHYSCGG----VVTDPYGHTE
Mb1578|M.bovis_AF2122/97 KLPFVRELCRDYQH--IDPVVELVPVRPVVHYMMGG----VHTDINGATT
Rv1552|M.tuberculosis_H37Rv KLPFVRELCRDYQH--IDPVVELVPVRPVVHYMMGG----VHTDINGATT
Mflv_3157|M.gilvum_PYR-GCK AQPNYFLPLDKAG---IDPFVTAYPLRMVYEGSVRGTGGLRLVGPDCATS
MSMEG_5532|M.smegmatis_MC2_155 AQPNYFLPLDKAG---IDPFTTAYPVRMVHEGSVRGTGGLRLTGPDCATT
Mvan_0484|M.vanbaalenii_PYR-1 LYEVSPTVRRAFTEFEVDPSTDLLEVTPFIVLGTSSTSG-PTIDLDGATT
** : . . :
MLBr_01226|M.leprae_Br4923 LAGLFAAGEVARTGMHGANRLA--SNSLLEGLVVGGRAGRAAAAHAAAAG
MAV_3190|M.avium_104 LPGLFAAGEVARTGLHGANRLA--SNSLLEGLVVGGRAGRAAAAHAAEAG
MMAR_2392|M.marinum_M LPGLFAAGEVARTGMHGANRLA--SNSLLEGLVVGGRAGKGAAAHAAAAG
MUL_1569|M.ulcerans_Agy99 LPGLFAAGEVARTGMHGANRLA--SNSLLEGLVVGGRAGKGAAAHAAAAG
TH_0808|M.thermoresistible__bu VPGLFAAGECARTGMHGANRLA--SNSLLEGLVMGX----XAAPRSAR--
MAB_2673c|M.abscessus_ATCC_199 VAGLFAAGEVARTGMHGANRLA--SNSLLEGLVVGRRAGEAAARIADRAG
Mb1578|M.bovis_AF2122/97 LPGLYAAGETACVSINGANRLG--SNSLPELLVFGARAGRAAADYAARHQ
Rv1552|M.tuberculosis_H37Rv LPGLYAAGETACVSINGANRLG--SNSLPELLVFGARAGRAAADYAARHQ
Mflv_3157|M.gilvum_PYR-GCK VPGLYAAGDAATRELITGAISGGGSHNGSWAIASGSFAGRGAAEYSRRRT
MSMEG_5532|M.smegmatis_MC2_155 VPGLYAAGDAATRELITGSRSGGGSHNGSWAIASGTFAGRGAAEFSRRRT
Mvan_0484|M.vanbaalenii_PYR-1 VPGLYAAGYGTACPHLMSGISG--SGISSFSAVAGYRAGTAAAARGGHAV
:.**:*** : . . * . * ** .
MLBr_01226|M.leprae_Br4923 RAY----------------------VSKLEPVTHHALKRRELQRVMSRDA
MAV_3190|M.avium_104 RPR----------------------ALAPEPVAHTAPPRAELQAAMSRDA
MMAR_2392|M.marinum_M LAH----------------------ATMPEPIAHTAPRRDDLQRAMSRDA
MUL_1569|M.ulcerans_Agy99 LAH----------------------ATMPEPIAHTAPRRDDLQRAMSRDA
TH_0808|M.thermoresistible__bu --------------------------------------------------
MAB_2673c|M.abscessus_ATCC_199 VVD----------------------ERDPAPPR---LDRRRLQSAMTRWA
Mb1578|M.bovis_AF2122/97 KSDRGPSSAVRAQARTEALRLERELSRHGQGGERIADIRADMQATLESAA
Rv1552|M.tuberculosis_H37Rv KSDRGPSSAVRAQARTEALRLERELSRHGQGGERIADIRADMQATLESAA
Mflv_3157|M.gilvum_PYR-GCK FAGFVEVDGVG-----------LRPGPQH-APTVDDVVRVAQDHILPPMR
MSMEG_5532|M.smegmatis_MC2_155 TRAARAGAESP-----------LNVRTRNGAPTVDEVVRAAQQHVLPPLR
Mvan_0484|M.vanbaalenii_PYR-1 ARSVWENQAQES------FAEYFAPMRRLRQVRPVDVWKAIGEATANPAF
MLBr_01226|M.leprae_Br4923 AVMRNAAGLQRLSDTLAEAPIRHVTGR-------------RDFEDVALTL
MAV_3190|M.avium_104 SVVRDAAGLTRLSATLSQAPARSIGGR-------------RDFEDVALAV
MMAR_2392|M.marinum_M SVVRDAAGLHRLAEMLDG-PTRGIADR-------------NDFEDVALAL
MUL_1569|M.ulcerans_Agy99 SVVRDAAGLHRLAEMLDG-PTRGIADR-------------NDFEDVALAL
TH_0808|M.thermoresistible__bu --------------------------------------------------
MAB_2673c|M.abscessus_ATCC_199 SVLRDADGLGLLEAELRCGTVSPVDPARMTP---------RDVEDAALTL
Mb1578|M.bovis_AF2122/97 GIYRDGPTLTKAVEEIRVLQERFATAGIDDHSRTFNTELTALLELSGMLD
Rv1552|M.tuberculosis_H37Rv GIYRDGPTLTKAVEEIRVLQERFATAGIDDHSRTFNTELTALLELSGMLD
Mflv_3157|M.gilvum_PYR-GCK SHLKSQVRLTESAGVLEQLWTDIAAGLAPVPAR----DAYKPRQAVALVA
MSMEG_5532|M.smegmatis_MC2_155 SYLKTAERLAESAAALETVWRDIADGLAPVAPR----DTYKVRQAVALVA
Mvan_0484|M.vanbaalenii_PYR-1 ALFKSEARIDSALAELYRIRDRVLRYVFAPDYQ----ELKKAHEVRAYLT
MLBr_01226|M.leprae_Br4923 TARAVAAAALARNESRGCHHCTEYPDTAPEHARSTVIRLADDQNLVRAEA
MAV_3190|M.avium_104 AARAVAAAALARTESRGCHHREEHPQAAPEQARSNLLRLADDQNSVLVQA
MMAR_2392|M.marinum_M AARVVGAGALARNESRGCHHRAEYPDVVPEQGRGIVVRLAGDQDTVCVEA
MUL_1569|M.ulcerans_Agy99 AARVVGAGALARNESRGCHHRAEYPDVVPEQGRGIVVRLAGDQDTVCVEA
TH_0808|M.thermoresistible__bu --------------------------------------------------
MAB_2673c|M.abscessus_ATCC_199 TAQAVATAAAARTESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPS
Mb1578|M.bovis_AF2122/97 VALAIVESGLRREESRGAHQRTDFPNRDDEHFLAHTLVHRESDGTLRVGY
Rv1552|M.tuberculosis_H37Rv VALAIVESGLRREESRGAHQRTDFPNRDDEHFLAHTLVHRESDGTLRVGY
Mflv_3157|M.gilvum_PYR-GCK AARWITAASLARAETRGIHRREDLPGTDHRLSHRILVGGLDTVWTIPDPV
MSMEG_5532|M.smegmatis_MC2_155 AARWVTAASLARAETRGLHRREDIPDTDARFDHRILVGGLDEVWTAPDPV
Mvan_0484|M.vanbaalenii_PYR-1 LAIITCEAMKRRTESRGELFRIDYPYADNDEWLKWLLVRRGSGGEFDLQF
MLBr_01226|M.leprae_Br4923 LATVG-------------------------------
MAV_3190|M.avium_104 LAAVV-------------------------------
MMAR_2392|M.marinum_M LAAVG-------------------------------
MUL_1569|M.ulcerans_Agy99 LAAVG-------------------------------
TH_0808|M.thermoresistible__bu ------------------------------------
MAB_2673c|M.abscessus_ATCC_199 GVPC--------------------------------
Mb1578|M.bovis_AF2122/97 LPVTITRWPPGERVYGR-------------------
Rv1552|M.tuberculosis_H37Rv LPVTITRWPPGERVYGR-------------------
Mflv_3157|M.gilvum_PYR-GCK LPELISSEAVA-------------------------
MSMEG_5532|M.smegmatis_MC2_155 APRLVS-EAVA-------------------------
Mvan_0484|M.vanbaalenii_PYR-1 SERDLPIQSWPVQAPPGRHPSPYTVPQGYREEAAVQ