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KVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNVETRPGQGYPRTYEDQEKWRGGWRLDGR GRLRLRDGGRLAKLARIFANPKLPSIDDYYEPWTYDYENLTSAPLGEHMPVAPPRSLIDGKPMKVSWSAA WDDDLGGSQEIAPGDPVLQKVSEHIKLEFEQTFMFYLPRICEHCLNPSCVASCPSGAMYKRSEDGIVLVD QDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYPRIEVGMPTVCSETCVGRLRYLGLVLYDVDRVLEA ASVPDETDLYEAHRQILLDPTDPAVIAGARAEGISDEWIEAAQHSPVYKLIHTYGVALPLHPEFRTVPMV WYIPPLSPVVDAVSRDGHDGEDLGNLFGALHALRIPLEYLAGLFTAGDTRVVEGVLQRLAAMRSYMRDIN LGRETQPHIPASVGMTEQQIYDMYRLLALAKYEERYVIPTAYGTDGREVEEPTCALSFDGGPGMYESGPF GEASGGPVPVAVETFHALQHRQTSDGMAANAEHPSRVNLLNWDGRGVPAGMFPEDSQ*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5139 | narH | - | 100% (548) | nitrate reductase, beta subunit |
| M. smegmatis MC2 155 | MSMEG_1847 | - | 4e-17 | 37.04% (108) | 4Fe-4S ferredoxin, iron-sulfur binding protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1194 | narH | 0.0 | 80.04% (551) | respiratory nitrate reductase subunit beta NarH |
| M. gilvum PYR-GCK | Mflv_2157 | - | 0.0 | 85.37% (547) | nitrate reductase, beta subunit |
| M. tuberculosis H37Rv | Rv1162 | narH | 0.0 | 80.04% (551) | respiratory nitrate reductase subunit beta NarH |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_5196 | hybA | 2e-17 | 44.44% (81) | Fe-S-cluster-containing hydrogenase, HybA |
| M. avium 104 | MAV_1304 | narH | 0.0 | 79.82% (545) | nitrate reductase, beta subunit |
| M. thermoresistible (build 8) | TH_1884 | narH | 0.0 | 83.79% (543) | PROBABLE RESPIRATORY NITRATE REDUCTASE (BETA CHAIN) NARH |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_4545 | - | 0.0 | 86.11% (547) | nitrate reductase, beta subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2157|M.gilvum_PYR-GCK MKVMAQVAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
Mvan_4545|M.vanbaalenii_PYR-1 MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
MSMEG_5139|M.smegmatis_MC2_155 MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNVETRPGQ
Mb1194|M.bovis_AF2122/97 MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGV
Rv1162|M.tuberculosis_H37Rv MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGV
MAV_1304|M.avium_104 MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQ
TH_1884|M.thermoresistible__bu ---MTQLAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
MMAR_5196|M.marinum_M ---MSSNSFYGPLDDPAG--------DAGYTDHP-PRVGFFT--DTSICI
*:. :: **. * . .:*::. .. :*. :*
Mflv_2157|M.gilvum_PYR-GCK GYPRTYQDQERWRGGWVRDKRGRLKLRGGGRLNKLLNIFSNPKMPSIDDY
Mvan_4545|M.vanbaalenii_PYR-1 GYPRTYQDQERWRGGWIRDKRGRLRLRGGGRLNKLLNIFSNPKLPSINDY
MSMEG_5139|M.smegmatis_MC2_155 GYPRTYEDQEKWRGGWRLDGRGRLRLRDGGRLAKLARIFANPKLPSIDDY
Mb1194|M.bovis_AF2122/97 GYPRTYEDQERWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDY
Rv1162|M.tuberculosis_H37Rv GYPRTYEDQERWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDY
MAV_1304|M.avium_104 GYPRTYEDQERWRGGWIRDKRGRLRLRDGGRFQKLLRIFANPKLPVIGDY
TH_1884|M.thermoresistible__bu GYPRTYEDQERWRGGWVRDRRGRLRLRDGGRFAKLLRIFANPKMPSINDY
MMAR_5196|M.marinum_M GCKACEVACKEWN-----------EVPDDG-FNLLGMSFDNTGMLGASTW
* :.*. .: ..* : * * *. : . :
Mflv_2157|M.gilvum_PYR-GCK YEPWTYDYENLINAPLGDQMPVAPPRSLITGEPMKVEWSANWDDNLGGST
Mvan_4545|M.vanbaalenii_PYR-1 YEPWTYDYENLTNAPLGEHMPVAPPRSLISGKPMKVEWSANWDDDLGGSP
MSMEG_5139|M.smegmatis_MC2_155 YEPWTYDYENLTSAPLGEHMPVAPPRSLIDGKPMKVSWSAAWDDDLGGSQ
Mb1194|M.bovis_AF2122/97 YEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSP
Rv1162|M.tuberculosis_H37Rv YEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSP
MAV_1304|M.avium_104 YEPWTYEYENLTTAPAGDTFPTAAPKSLISGEPMKVSWGPNWDDNLAGSP
TH_1884|M.thermoresistible__bu YEPWTYDYDNLLSAPLGDQMPVAPPRSLISGAPMTIEWSANWDDDLGGSP
MMAR_5196|M.marinum_M RH---VAFIEQARPPVDLGVPKFERPGQASGPQDRTDFR-----------
. : : .* . .* . * .:
Mflv_2157|M.gilvum_PYR-GCK EILSGDPILEKVS---ETVRLELEQTFMFYLPRICEHCLNPSCVASCPSG
Mvan_4545|M.vanbaalenii_PYR-1 EIVPGDPVLAKVS---EEIRLELEQTFMFYLPRICEHCLNPSCVASCPSG
MSMEG_5139|M.smegmatis_MC2_155 EIAPGDPVLQKVS---EHIKLEFEQTFMFYLPRICEHCLNPSCVASCPSG
Mb1194|M.bovis_AF2122/97 EIVPNDPVLKKVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSG
Rv1162|M.tuberculosis_H37Rv EIVPNDPVLKKVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSG
MAV_1304|M.avium_104 EILAEDPILKKVS---EEVKLSLEQTFMFYLPRICEHCLNPSCVASCPSG
TH_1884|M.thermoresistible__bu EIVPDDPVLKKVN---EQVKLELEQTFMFYLPRICEHCLNPSCVASCPSG
MMAR_5196|M.marinum_M ---------------------------WLMSSDVCKHCTHAGCLDVCPTG
: . :*:** :..*: **:*
Mflv_2157|M.gilvum_PYR-GCK AMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
Mvan_4545|M.vanbaalenii_PYR-1 AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
MSMEG_5139|M.smegmatis_MC2_155 AMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
Mb1194|M.bovis_AF2122/97 AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
Rv1162|M.tuberculosis_H37Rv AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
MAV_1304|M.avium_104 AMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTLCYP
TH_1884|M.thermoresistible__bu ALYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAEKCTFCYP
MMAR_5196|M.marinum_M ALFR-TEFGTVVVQQDICNGCGYCVSGCPYGVIERREGDGRAWKCTLCYD
*::: :* * *:*:** *.* ******* : .. *:* ***:**
Mflv_2157|M.gilvum_PYR-GCK RIEVGLPTVCSETCVGRLRYLGVVLYDVDRVTEAASVEKDTDLYEAHRSI
Mvan_4545|M.vanbaalenii_PYR-1 RIEVGLPTVCSETCVGRLRYLGLVLYDVDRVTAAASVENDTDLYEAQKSI
MSMEG_5139|M.smegmatis_MC2_155 RIEVGMPTVCSETCVGRLRYLGLVLYDVDRVLEAASVPDETDLYEAHRQI
Mb1194|M.bovis_AF2122/97 RIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRI
Rv1162|M.tuberculosis_H37Rv RIEVGLPTVCSETCVGRLRYLGLVLYDVDQVLQAASVESDTDLYEAQRRI
MAV_1304|M.avium_104 RMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDI
TH_1884|M.thermoresistible__bu RVEVGLPTVCSETCVGRLRYIGLVLYDADAVTAAASVEKETDLYEAQKAL
MMAR_5196|M.marinum_M RLRDGLEPACAKACPTNSIQFGPLEELRERAARRVEQLHDRGVPEAR---
*:. *: . *:::* . :* : : . .. : .: .*:
Mflv_2157|M.gilvum_PYR-GCK LLDPNDPAVISGARAEGISDEWIEAAQRSPIYALIHTFGVALPLHPEYRT
Mvan_4545|M.vanbaalenii_PYR-1 LLDPSDPEVIAGARAEGISDEWIEAAQRSPIYALIHEYQVALPLHPEYRT
MSMEG_5139|M.smegmatis_MC2_155 LLDPTDPAVIAGARAEGISDEWIEAAQHSPVYKLIHTYGVALPLHPEFRT
Mb1194|M.bovis_AF2122/97 LLDPHDPRVIAGARAEGIADEWIEAAQRSPVYALINTYRVALPLHPEYRT
Rv1162|M.tuberculosis_H37Rv LLDPHDPRVIAGARAEGIADEWIEAAQRSPVYALINTYRVALPLHPEYRT
MAV_1304|M.avium_104 LLDPNDTDVIAGARAQGIPDEWIEAAQRSPVYALIHTYRVALPLHPEYRT
TH_1884|M.thermoresistible__bu ILDPNDPQVIAAARAEGIPDDWIEAAQRSPVYALIHEYQVALPLHPEYRT
MMAR_5196|M.marinum_M -LYGHDP-----NDGVGGNGAFFLLLDEPEVYGLPPD-----PVVPTRDT
* *. . * . :: :.. :* * *: * *
Mflv_2157|M.gilvum_PYR-GCK VPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALEALRIPIEYLAGLFTA
Mvan_4545|M.vanbaalenii_PYR-1 MPMVWYIPPLSPVVDAVSRDGHDGEDAGNLFGALEALRIPIEYLAGLFTA
MSMEG_5139|M.smegmatis_MC2_155 VPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALHALRIPLEYLAGLFTA
Mb1194|M.bovis_AF2122/97 MPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTA
Rv1162|M.tuberculosis_H37Rv MPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPIAYLAELFTA
MAV_1304|M.avium_104 MPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTA
TH_1884|M.thermoresistible__bu MPMVWYIPPLSPVVDAVARDGHDGEDLGNLFGALSALRIPMQYLAGLFTA
MMAR_5196|M.marinum_M PAMWRYAGMAASALVAIAVSAFAGRGRP----------------------
.* * :..: *:: ... *..
Mflv_2157|M.gilvum_PYR-GCK GDTAVVEGVLRRLAAMRSYMRDVNLGRETQPHIPESVGMTEEQIYQMYRL
Mvan_4545|M.vanbaalenii_PYR-1 GDTAVVEGVLRRLAAMRSYMRDVNLGRETQPHIPESVGMTEEQIYEMYRL
MSMEG_5139|M.smegmatis_MC2_155 GDTRVVEGVLQRLAAMRSYMRDINLGRETQPHIPASVGMTEQQIYDMYRL
Mb1194|M.bovis_AF2122/97 GDTEVVAGVLRRLAAMRCYMRDINLGRETQPHIPESVGMTEEQIYQMYRL
Rv1162|M.tuberculosis_H37Rv GDTEVVAGVLRRLAAMRCYMRDINLGRETQPHIPESVGMTEEQIYQMYRL
MAV_1304|M.avium_104 GDTAVVEGVLRRLAAMRSYMRDINLGRETQPHIPHSVGMTEEQIYEMYRL
TH_1884|M.thermoresistible__bu GDTAVVEAALRRLAAMRSYMRDINLGRPTQPHIPESVGLTEEQVYNMYRL
MMAR_5196|M.marinum_M --------------------------------------------------
Mflv_2157|M.gilvum_PYR-GCK LALAKYEERYVIPTAYAA--VEPLSMEESGCSLSFEGGPGMYDSGPFGEA
Mvan_4545|M.vanbaalenii_PYR-1 LALAKYDERYVIPTAYAA--VEPWSLEEPGCSLSFEGGPGMYESGPFGEA
MSMEG_5139|M.smegmatis_MC2_155 LALAKYEERYVIPTAYG---TDGREVEEPTCALSFDGGPGMYESGPFGEA
Mb1194|M.bovis_AF2122/97 LAVAKYEERYVIPTSYAGELPAAAMTDDMGCSLSVDGGPGMYESGPFGQG
Rv1162|M.tuberculosis_H37Rv LAVAKYEERYVIPTSYAGELPAAAMTDDMGCSLSVDGGPGMYESGPFGQG
MAV_1304|M.avium_104 LAIAKYEERYVIPTAFA---PQARDLEQMGCSLTGDGGPGMYESE-----
TH_1884|M.thermoresistible__bu LALAKYDERYVIPTAYPA--TVPGAVEEAGCSLDFEGGPGMYESGPFGEA
MMAR_5196|M.marinum_M --------------------------------------------------
Mflv_2157|M.gilvum_PYR-GCK SGQPVPVAVETFHALKQRQTSEHMAANESRPSRVNLLNWDGRGAPSGLFP
Mvan_4545|M.vanbaalenii_PYR-1 SGQPIPVAVETFHALRQRQTSEHMAADEARPSRVNLLNWDGRGAPSGLFP
MSMEG_5139|M.smegmatis_MC2_155 SGGPVPVAVETFHALQHRQTSDGMAANAEHPSRVNLLNWDGRGVPAGMFP
Mb1194|M.bovis_AF2122/97 SPTPVPIAVESFHALQHAG--SAATGGAGR-SRVNLLNWDPNGAAAGLFP
Rv1162|M.tuberculosis_H37Rv SPTPVPIAVESFHALQHAG--SAATGGAGR-SRVNLLNWDPNGAAAGLFP
MAV_1304|M.avium_104 -----PVAVETFHALKQRGGPTEAEPGSTR-PRVNLLNWDGGQVPAGMFP
TH_1884|M.thermoresistible__bu SGDPVPVAVETFHALRHRQTSGGMAANASRPSRVNLLNWDGRGVPAGMFP
MMAR_5196|M.marinum_M --------------------------------------------------
Mflv_2157|M.gilvum_PYR-GCK RNGDR------
Mvan_4545|M.vanbaalenii_PYR-1 QDGDR------
MSMEG_5139|M.smegmatis_MC2_155 EDSQ-------
Mb1194|M.bovis_AF2122/97 EPQPSKDVVQR
Rv1162|M.tuberculosis_H37Rv EPQPSKDVVQR
MAV_1304|M.avium_104 EAQRR------
TH_1884|M.thermoresistible__bu DAEST------
MMAR_5196|M.marinum_M -----------