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MSSNSFYGPLDDPAGDAGYTDHPPRVGFFTDTSICIGCKACEVACKEWNEVPDDGFNLLGMSFDNTGMLG ASTWRHVAFIEQARPPVDLGVPKFERPGQASGPQDRTDFRWLMSSDVCKHCTHAGCLDVCPTGALFRTEF GTVVVQQDICNGCGYCVSGCPYGVIERREGDGRAWKCTLCYDRLRDGLEPACAKACPTNSIQFGPLEELR ERAARRVEQLHDRGVPEARLYGHDPNDGVGGNGAFFLLLDEPEVYGLPPDPVVPTRDTPAMWRYAGMAAS ALVAIAVSAFAGRGRP
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_5196 | hybA | - | 100% (296) | Fe-S-cluster-containing hydrogenase, HybA |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1194 | narH | 1e-18 | 46.91% (81) | respiratory nitrate reductase subunit beta NarH |
| M. gilvum PYR-GCK | Mflv_2157 | - | 1e-18 | 37.69% (130) | nitrate reductase, beta subunit |
| M. tuberculosis H37Rv | Rv1162 | narH | 1e-18 | 46.91% (81) | respiratory nitrate reductase subunit beta NarH |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. avium 104 | MAV_0423 | - | 1e-105 | 61.15% (314) | 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_1847 | - | 1e-157 | 86.49% (296) | 4Fe-4S ferredoxin, iron-sulfur binding protein |
| M. thermoresistible (build 8) | TH_1884 | narH | 7e-18 | 45.68% (81) | PROBABLE RESPIRATORY NITRATE REDUCTASE (BETA CHAIN) NARH |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_4545 | - | 1e-18 | 46.91% (81) | nitrate reductase, beta subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2157|M.gilvum_PYR-GCK MKVMAQVAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
Mvan_4545|M.vanbaalenii_PYR-1 MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
TH_1884|M.thermoresistible__bu ---MTQLAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNVETRPGQ
Mb1194|M.bovis_AF2122/97 MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGV
Rv1162|M.tuberculosis_H37Rv MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGV
MMAR_5196|M.marinum_M --------MSSN---SFYGPLDDPAGDAGYT-DHPPRVGFFT--DTSICI
MSMEG_1847|M.smegmatis_MC2_155 --------MSENRRNSFYGPLEDPATDAGYDDEHPPRVGFFT--DTSVCI
MAV_0423|M.avium_104 --------MGKGELMGQLTGPTDPAADAGWA-DRKPRKGFFT--DTSICI
* . . : . .: .. :*. :*
Mflv_2157|M.gilvum_PYR-GCK GYPRTYQDQERWRGGWVRDKRGRLKLRGGGRLNKLLNIFSNPKMPSIDDY
Mvan_4545|M.vanbaalenii_PYR-1 GYPRTYQDQERWRGGWIRDKRGRLRLRGGGRLNKLLNIFSNPKLPSINDY
TH_1884|M.thermoresistible__bu GYPRTYEDQERWRGGWVRDRRGRLRLRDGGRFAKLLRIFANPKMPSINDY
Mb1194|M.bovis_AF2122/97 GYPRTYEDQERWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDY
Rv1162|M.tuberculosis_H37Rv GYPRTYEDQERWRGGWVRDKKGRLRLRDGGRIHKLLRIFANPKLPTIGDY
MMAR_5196|M.marinum_M GCKACEVACKEWNE---VPDDG-FNLLG----------------------
MSMEG_1847|M.smegmatis_MC2_155 GCKACEVACKEWNG---VPDDG-FNLLG----------------------
MAV_0423|M.avium_104 GCKACEVACKEWNR---NPQDGDLELLG----------------------
* :.*. * :.* .
Mflv_2157|M.gilvum_PYR-GCK YEPWTYDYENLINAPLGDQMPVAPPRSLITGEPMKVEWSANWDDNLGGST
Mvan_4545|M.vanbaalenii_PYR-1 YEPWTYDYENLTNAPLGEHMPVAPPRSLISGKPMKVEWSANWDDDLGGSP
TH_1884|M.thermoresistible__bu YEPWTYDYDNLLSAPLGDQMPVAPPRSLISGAPMTIEWSANWDDDLGGSP
Mb1194|M.bovis_AF2122/97 YEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSP
Rv1162|M.tuberculosis_H37Rv YEPWTYDYENLTSAPAGDTFPTAAPRSLISGNPMKVSWGSNWDDNLAGSP
MMAR_5196|M.marinum_M ---MSFDNTGMLGASTWRHVAFIEQ---------ARP-----PVDLGVPK
MSMEG_1847|M.smegmatis_MC2_155 ---MSFDNTGNLGANSWRHVAFIEQ---------PAPEPGRTTEDLGMPG
MAV_0423|M.avium_104 ---SSYDNTGALGASTWRHVAFVEQG---RERIEQARESGRALIDLGMP-
::* . .* .. :*. .
Mflv_2157|M.gilvum_PYR-GCK EILSGDPILEKVS---ETVRLELEQTFMFYLPRICEHCLNPSCVASCPSG
Mvan_4545|M.vanbaalenii_PYR-1 EIVPGDPVLAKVS---EEIRLELEQTFMFYLPRICEHCLNPSCVASCPSG
TH_1884|M.thermoresistible__bu EIVPDDPVLKKVN---EQVKLELEQTFMFYLPRICEHCLNPSCVASCPSG
Mb1194|M.bovis_AF2122/97 EIVPNDPVLKKVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSG
Rv1162|M.tuberculosis_H37Rv EIVPNDPVLKKVNQVNQEVKLKLEETFMFYLPRICEHCLNPSCVASCPSG
MMAR_5196|M.marinum_M FERPGQASGPQD----------RTDFRWLMSSDVCKHCTHAGCLDVCPTG
MSMEG_1847|M.smegmatis_MC2_155 FERPGDATGPET----------RQDFRWLMASDVCKHCTHAGCLDVCPTG
MAV_0423|M.avium_104 -TLPGAASEPTEP-----AVPHTPEFRWLMSSDVCKHCTHAGCLDVCPTG
.. . : : . :*:** :..*: **:*
Mflv_2157|M.gilvum_PYR-GCK AMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHK------------
Mvan_4545|M.vanbaalenii_PYR-1 AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHK------------
TH_1884|M.thermoresistible__bu ALYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHK------------
Mb1194|M.bovis_AF2122/97 AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHK------------
Rv1162|M.tuberculosis_H37Rv AMYKRTEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHK------------
MMAR_5196|M.marinum_M ALFR-TEFGTVVVQQDICNGCGYCVSGCPYGVIERREG------------
MSMEG_1847|M.smegmatis_MC2_155 ALFR-TEFSTVVVQQDICNGCGYCVSGCPYGVIERREG------------
MAV_0423|M.avium_104 ALFR-TEFGTVVVQHDVCNGCGTCVAGCPFGVVERRNDGTYATPAQRPDR
*::: :* . *:*::* *.* **:***: : ..
Mflv_2157|M.gilvum_PYR-GCK ------TGKAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGVVLYDVDRVT
Mvan_4545|M.vanbaalenii_PYR-1 ------TGKAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDRVT
TH_1884|M.thermoresistible__bu ------TGKAEKCTFCYPRVEVGLPTVCSETCVGRLRYIGLVLYDADAVT
Mb1194|M.bovis_AF2122/97 ------TGKAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVL
Rv1162|M.tuberculosis_H37Rv ------TGKAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVLYDVDQVL
MMAR_5196|M.marinum_M ------DGRAWKCTLCYDRLRDGLEPACAKACP-----------------
MSMEG_1847|M.smegmatis_MC2_155 ------DGRAWKCTLCYDRLHDGLEPACAKACP-----------------
MAV_0423|M.avium_104 PSEEIVTGVAQKCTLCYDRLLEDEIPACAKTCP-----------------
* * ***:** *: . ..*:::*
Mflv_2157|M.gilvum_PYR-GCK EAASVEKDTDLYEAHRSILLDPNDPAVISGARAEGISDEWIEAAQRSPIY
Mvan_4545|M.vanbaalenii_PYR-1 AAASVENDTDLYEAQKSILLDPSDPEVIAGARAEGISDEWIEAAQRSPIY
TH_1884|M.thermoresistible__bu AAASVEKETDLYEAQKALILDPNDPQVIAAARAEGIPDDWIEAAQRSPVY
Mb1194|M.bovis_AF2122/97 QAASVESDTDLYEAQRRILLDPHDPRVIAGARAEGIADEWIEAAQRSPVY
Rv1162|M.tuberculosis_H37Rv QAASVESDTDLYEAQRRILLDPHDPRVIAGARAEGIADEWIEAAQRSPVY
MMAR_5196|M.marinum_M --------TNSIQFGPLEELRERAARRVEQLHDRGVPEARLYGHD-----
MSMEG_1847|M.smegmatis_MC2_155 --------TDSIQFGVLDELRERAARRVEELHERGVTEARLYGHD-----
MAV_0423|M.avium_104 --------TTSIKFGDHDDLVAMARRRVAALHAQGLTEARLYGAN-----
* : * : : .*:.: : . :
Mflv_2157|M.gilvum_PYR-GCK ALIHTFGVALPLHPEYRTVPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFG
Mvan_4545|M.vanbaalenii_PYR-1 ALIHEYQVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDAGNLFG
TH_1884|M.thermoresistible__bu ALIHEYQVALPLHPEYRTMPMVWYIPPLSPVVDAVARDGHDGEDLGNLFG
Mb1194|M.bovis_AF2122/97 ALINTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFG
Rv1162|M.tuberculosis_H37Rv ALINTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFG
MMAR_5196|M.marinum_M -----------------------------------PNDGVGGN--GAFFL
MSMEG_1847|M.smegmatis_MC2_155 -----------------------------------PNDGVGGD--GAFFL
MAV_0423|M.avium_104 -----------------------------------EHDGVGGT--GSIFL
.** .* * :*
Mflv_2157|M.gilvum_PYR-GCK ALEALRIPIEYLAGLFTAGDTAVVEGVLRRLAAMRSYMRDVNLGRETQPH
Mvan_4545|M.vanbaalenii_PYR-1 ALEALRIPIEYLAGLFTAGDTAVVEGVLRRLAAMRSYMRDVNLGRETQPH
TH_1884|M.thermoresistible__bu ALSALRIPMQYLAGLFTAGDTAVVEAALRRLAAMRSYMRDINLGRPTQPH
Mb1194|M.bovis_AF2122/97 ALDALRIPIAYLAELFTAGDTEVVAGVLRRLAAMRCYMRDINLGRETQPH
Rv1162|M.tuberculosis_H37Rv ALDALRIPIAYLAELFTAGDTEVVAGVLRRLAAMRCYMRDINLGRETQPH
MMAR_5196|M.marinum_M LLDE----------------------------------------------
MSMEG_1847|M.smegmatis_MC2_155 LLDE----------------------------------------------
MAV_0423|M.avium_104 LLDE----------------------------------------------
*.
Mflv_2157|M.gilvum_PYR-GCK IPESVGMTEEQIYQMYRLLALAKYEERYVIPTAYAAVEPLS--MEESGCS
Mvan_4545|M.vanbaalenii_PYR-1 IPESVGMTEEQIYEMYRLLALAKYDERYVIPTAYAAVEPWS--LEEPGCS
TH_1884|M.thermoresistible__bu IPESVGLTEEQVYNMYRLLALAKYDERYVIPTAYPATVPGA--VEEAGCS
Mb1194|M.bovis_AF2122/97 IPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMGCS
Rv1162|M.tuberculosis_H37Rv IPESVGMTEEQIYQMYRLLAVAKYEERYVIPTSYAGELPAAAMTDDMGCS
MMAR_5196|M.marinum_M -PEVYGLPPDPVVPTRDTPAMWRYAG------------------------
MSMEG_1847|M.smegmatis_MC2_155 -PEVYGLPPDPVVPTRDAGAMWRYAG------------------------
MAV_0423|M.avium_104 -PEVYGLPPDPRVCTADLMTMFKRAG------------------------
** *:. : :: :
Mflv_2157|M.gilvum_PYR-GCK LSFEGGPGMYDSGPFGEASGQPVPVAVETFHALKQRQTSEHMAANESRPS
Mvan_4545|M.vanbaalenii_PYR-1 LSFEGGPGMYESGPFGEASGQPIPVAVETFHALRQRQTSEHMAADEARPS
TH_1884|M.thermoresistible__bu LDFEGGPGMYESGPFGEASGDPVPVAVETFHALRHRQTSGGMAANASRPS
Mb1194|M.bovis_AF2122/97 LSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQH---AGSAATGGAGRS
Rv1162|M.tuberculosis_H37Rv LSVDGGPGMYESGPFGQGSPTPVPIAVESFHALQH---AGSAATGGAGRS
MMAR_5196|M.marinum_M ----------------MAASALVAIAVSAFAGRGRP--------------
MSMEG_1847|M.smegmatis_MC2_155 ----------------MAAGALVGIAVSAFVGRRT---------------
MAV_0423|M.avium_104 ----------------LAAAGMVGAAVLAFWRSR----------------
.: : ** :*
Mflv_2157|M.gilvum_PYR-GCK RVNLLNWDGRGAPSGLFPRNGDR------
Mvan_4545|M.vanbaalenii_PYR-1 RVNLLNWDGRGAPSGLFPQDGDR------
TH_1884|M.thermoresistible__bu RVNLLNWDGRGVPAGMFPDAEST------
Mb1194|M.bovis_AF2122/97 RVNLLNWDPNGAAAGLFPEPQPSKDVVQR
Rv1162|M.tuberculosis_H37Rv RVNLLNWDPNGAAAGLFPEPQPSKDVVQR
MMAR_5196|M.marinum_M -----------------------------
MSMEG_1847|M.smegmatis_MC2_155 -----------------------------
MAV_0423|M.avium_104 -----------------------------