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TVGIVVVSHSRPLARAAVGLAQEMLHGKAVRISIAAGLDDTTLGTDASAILEAITSADSGDGVLVLMDLG SAVLSAELALELLDDDVRARTVLCPAPLVEGLVVAAVAAAGDAALDEVAAEAAGALAGKAAHLEPATPPA PADDTAEPDELTGSFVVNNPHGLHARPAARFVAEVRRRDARVRIRNPRVDSPWVDGGSLSRIATLAVRCG DEVELRVSGGQAHETLQHLLSLAARNFDEDHTEAPPAAPATAHTPIGAGPGMGVGPAYSARSQTPDVPDA PAADPAAEWRRLGTAIADTRRTIGALRAATSRDIGEAEASIFDAHQLLLDDDALLGAARARIEQGENAAA AWSQAVAALAAEFDALPDPYLQARAEDVRAVGDQVLRAMLGTEGDAAAASGVLVAGDLTPAEAAELDPQR VVAVLLAFGSPHAHNVILLRAKGIPVIVGAGPGVLGIPDGTVVAVDATRGEFIVDPPADVREQFRHEAET LTRRRQAALQRAGEPAVTRGGRTVAVGANIAGVEDARAAAALGADHAGLIRTEFLFLGRRQAPDVDEQLA VYRKIAESLGDRRMTLRTLDVGGDKPLEFLPAAAELNPYLGVRGIRLSLAHPDVFSDQLLAMAKLAQDIP LSVMFPMVTTLDELFAARRLLDDAVRRAGPRPPTGLQVGMMVEVPAAALKSAAFSRHVDFLSIGTNDLSQ YALAADRNNDAVAGLGDTFDPGLLHLIAATCRGAAGAASVSVCGEFAADARAVPLLIGLGVDALSVTPPA IPTTKEAVRAVDDRHATETAEAALAAAGPAAVRDLLG*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2121 | - | - | 100% (808) | multiphosphoryl transfer protein (MTP) |
| M. smegmatis MC2 155 | MSMEG_0088 | ptsI | 1e-77 | 37.63% (558) | phosphoenolpyruvate-protein phosphotransferase |
| M. smegmatis MC2 155 | MSMEG_3934 | - | 2e-27 | 29.22% (421) | phosphoenolpyruvate synthase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. gilvum PYR-GCK | Mflv_0749 | - | 2e-73 | 38.54% (493) | phosphoenolpyruvate-protein phosphotransferase |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_3410 | - | 3e-18 | 23.66% (448) | pyruvate dikinase |
| M. avium 104 | - | - | - | - | - |
| M. thermoresistible (build 8) | TH_1154 | - | 2e-28 | 28.44% (429) | phosphoenolpyruvate synthase |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_0097 | - | 8e-74 | 37.34% (533) | phosphoenolpyruvate-protein phosphotransferase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_3410|M.marinum_M MSSFS--YVRFFEEFGIEDVPLVGGKNASLGEMFQKLSQQGVRVPHGFAT
TH_1154|M.thermoresistible__bu MDHVADRFVRDIAELRITDAEEAGGKGANMGEMVAAALP----VPPGFVL
Mflv_0749|M.gilvum_PYR-GCK ----------------------------------------------MFGF
Mvan_0097|M.vanbaalenii_PYR-1 ------------------------------------MWVLRQINVDIVGF
MSMEG_2121|M.smegmatis_MC2_155 ----MTVGIVVVSHSRPLARAAVGLAQEMLHGKAVRISIAAGLDDTTLGT
.
MMAR_3410|M.marinum_M TADAYKHMLDRAGAWDALHAELDD----LDADDVTALARKGKRAREIVYG
TH_1154|M.thermoresistible__bu LRDCYLESMQAGGVRSELAALHDEA--LAKVPDTEQLPALCERLQSLVRK
Mflv_0749|M.gilvum_PYR-GCK TPECVDLSTVSCN---------------LVHMTATTPSTS-LEVGAVLHG
Mvan_0097|M.vanbaalenii_PYR-1 TPECVDLSTVSCN---------------LVHMTASIPLTSHPTAGTVLHG
MSMEG_2121|M.smegmatis_MC2_155 DASAILEAITSADSGDGVLVLMDLGSAVLSAELALELLDDDVRARTVLCP
.. . . ::
MMAR_3410|M.marinum_M AGLPEDLASEIVAAYRIMQEEYGEDVSLAVRSSATAEDLPTASFAGQQET
TH_1154|M.thermoresistible__bu AGMADEVRKSVLAAYR----RLGPDAVVAVRSSATGEDGRDASFAGMNAT
Mflv_0749|M.gilvum_PYR-GCK VPVVPGVQYAPVIRPG----KLPDTDL-DAGGEIAEAERDAEK-------
Mvan_0097|M.vanbaalenii_PYR-1 VPVVGGVQYAPVIRPG----KLPSTDVGDTMPDLAEDRRAAEN-------
MSMEG_2121|M.smegmatis_MC2_155 APLVEGLVVAAVAAAG----DAALDEVAAEAAGALAGKAAHLEPATPPAP
. : : : .
MMAR_3410|M.marinum_M FLN-------------------IKGEESLLDACRRCFASLFTDRAIHYRV
TH_1154|M.thermoresistible__bu MTN-------------------VAGEDALLEAVVRCWMSLFSPRVITYRA
Mflv_0749|M.gilvum_PYR-GCK ---------------------------ARFTAAAATVADRLRERAAHATG
Mvan_0097|M.vanbaalenii_PYR-1 ---------------------------ERFAAAAATVAGRLRERAAHATG
MSMEG_2121|M.smegmatis_MC2_155 ADDTAEPDELTGSFVVNNPHGLHARPAARFVAEVRRRDARVRIRNPRVDS
: * . *
MMAR_3410|M.marinum_M DQGFDHFKVALSIGIMKMVRSDLASSGVMFTLDTESGFNDVVFVTGAYGL
TH_1154|M.thermoresistible__bu SRGFTD-EPAMAVVVQQMIASE--KSGVAFTADPSSGAQDRVVVEAAWGL
Mflv_0749|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_0097|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_2121|M.smegmatis_MC2_155 PWVDGGSLSRIATLAVRCGDEVELRVSGGQAHETLQHLLSLAARNFDEDH
MMAR_3410|M.marinum_M GENVVQGAVDPDEFYVHKPTYQAGHRAVLRRLIGDKAVKMVFVEGGTKQT
TH_1154|M.thermoresistible__bu GEVVVAGQVEPDTYVVAK-----EDLGVLDVRIGHKPFKIVRGPDGNDQ-
Mflv_0749|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_0097|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_2121|M.smegmatis_MC2_155 TEAPPAAPATAHTPIGAGPGMGVGPAYSARSQTPDVPDAPAADPAAEWRR
MMAR_3410|M.marinum_M TRNIPTPKADRAHFCITDEDVLELAGYALAIEQHYGRPMDIEWAKDGLDG
TH_1154|M.thermoresistible__bu --RVDLPDDQAHARVLDDRELRRIAELAIATEQHNGCPQDTEWAID-AAG
Mflv_0749|M.gilvum_PYR-GCK -------------------------AASEVLAATATLATDRGWLGA---A
Mvan_0097|M.vanbaalenii_PYR-1 -------------------------AASEVLAATATLAQDRGWLGA---A
MSMEG_2121|M.smegmatis_MC2_155 LGTAIADTRRTIGALRAATSRDIGEAEASIFDAHQLLLDDDALLGA---A
: * .
MMAR_3410|M.marinum_M KLYIVQARPETAASQRSMTTVETYVLEGSGEQREILTEGRSVGEKVASGV
TH_1154|M.thermoresistible__bu EAFLVQARPITTLRHAAAPTSEEHVT---------LARGLAAAPGLASGR
Mflv_0749|M.gilvum_PYR-GCK EKRIKDGAPAVRAVDGAIDQFVEMFTKLGG----LMAERVTDLRDIRDRV
Mvan_0097|M.vanbaalenii_PYR-1 EKRIKEGTPAVRAVNGAIDQFIEMFTKLGG----LMAERVTDLRDIRDRV
MSMEG_2121|M.smegmatis_MC2_155 RARIEQGENAAAAWSQAVAALAAEFDALPDP---YLQARAEDVRAVGDQV
. : :. . : . : : .
MMAR_3410|M.marinum_M VKRIDDLERLSDFKPGQ--VLVADTTTPDWEPVMK--IAAAIVTNRGGRT
TH_1154|M.thermoresistible__bu VRVLRSPEEGSQLQDGE--ILVAPMTNPDWLPTIR--RAAALVTDTGGMT
Mflv_0749|M.gilvum_PYR-GCK IAELNGLPEPGVPLPDEPSILCAEDLAPADTAGLDPSLVVALATTLGGPT
Mvan_0097|M.vanbaalenii_PYR-1 VAELNGLPEPGVPLPDEPSILCAEDLAPADTAGLDPALVVALATTLGGPT
MSMEG_2121|M.smegmatis_MC2_155 LRAMLGTEGDAAAASG---VLVAGDLTPAEAAELDPQRVVAVLLAFGSPH
: : . . . :* * * . : ..*: *.
MMAR_3410|M.marinum_M CHAAIIARELGIPAVVGAGDATTNVPDGQLVTVSCVEGDTGRVYRGE---
TH_1154|M.thermoresistible__bu CHAAIVARELGVPCVVGTRTATRDLHNG---TTVTVDGTHGRVVSGR---
Mflv_0749|M.gilvum_PYR-GCK SHTAIIARQLGIPCVVAVAG-LDDVPAG---TQVLVDGTRGTVTVSP---
Mvan_0097|M.vanbaalenii_PYR-1 SHTAIIARQLGIPCVVAVAG-LDDVPAG---AMVLVDGTRGTVTVGP---
MSMEG_2121|M.smegmatis_MC2_155 AHNVILLRAKGIPVIVGAGPGVLGIPDG---TVVAVDATRGEFIVDPPAD
.* .*: * *:* :*.. .: * : *:. * . .
MMAR_3410|M.marinum_M -------VGFHVDRTEVADLAR----------PRTEVMVNLGNPDLAFKT
TH_1154|M.thermoresistible__bu -------MASPAAAVTLAEPAARPALAAPAEVTATKIYVNLAMPDSAEEV
Mflv_0749|M.gilvum_PYR-GCK -DEDSARAEVAAARRTAEDAANWSGPGTTADGHAVAVLANVQDGAAARSA
Mvan_0097|M.vanbaalenii_PYR-1 -DEVSAREAVAEAKRAAETAAQWSGPGVTADGHAVAVLANVQDGAAARAA
MSMEG_2121|M.smegmatis_MC2_155 VREQFRHEAETLTRRRQAALQRAGEPAVTRGGRTVAVGANIAGVEDARAA
. : .*: * .
MMAR_3410|M.marinum_M SFLPNDGVGLARMEFIVGEYIK-VHPLALLHPDKVDDPEARRTIERLTRG
TH_1154|M.thermoresistible__bu AARGADGVGLLRAEFMLTEALGGRHPRDLIARGEQND-------------
Mflv_0749|M.gilvum_PYR-GCK RQTPAEGVGLFRTELCFLNTDS----------------------------
Mvan_0097|M.vanbaalenii_PYR-1 RQTPAEGVGLFRTELCFLNSDT----------------------------
MSMEG_2121|M.smegmatis_MC2_155 AALGADHAGLIRTEFLFLGRRQ----------------------------
: .** * *: .
MMAR_3410|M.marinum_M YSDGAEFFVQRLSEGIGTIAAAFWPKPVVVRMSDFKSNEYASLIGGSGFE
TH_1154|M.thermoresistible__bu -------LVQGMVNAVGRIAAAFAPRPVVYRATDFRTNEFRGLAGGDRYE
Mflv_0749|M.gilvum_PYR-GCK -----EPTVDEQADIYAEVLDAFAGNKVVIRTLDAGSDKPLKFAG----H
Mvan_0097|M.vanbaalenii_PYR-1 -----EPAVDEQATIYSEVLEAFAGNKVVIRTLDAGSDKPLKFAG----H
MSMEG_2121|M.smegmatis_MC2_155 -----APDVDEQLAVYRKIAESLGDRRMTLRTLDVGGDKPLEFLP----A
*: : :: . :. * * :: :
MMAR_3410|M.marinum_M PTESNPMIGFRGASRYAHPAYAEGFALECRAMQRVRDQMGLTNVVIMLPF
TH_1154|M.thermoresistible__bu PEEHNPMIGYRGCYRYIK--EPDVFALELQALARVREEN--PNVALMIPF
Mflv_0749|M.gilvum_PYR-GCK PDEANPALGVRGIRISFA--NPGLLDRQLEAIAAAGRRT-GNPPWVMAPM
Mvan_0097|M.vanbaalenii_PYR-1 PDEANPALGVRGVRIAAN--NPGLLDRQLEAIAAAGQRT-GNPPWVMAPM
MSMEG_2121|M.smegmatis_MC2_155 AAELNPYLGVRGIRLSLA--HPDVFSDQLLAMAKLAQ---DIPLSVMFPM
. * ** :* ** . : : *: :* *:
MMAR_3410|M.marinum_M VRRVAEADLVLETMAEH---GLRRGENGLKVYAMCEIPNNVILIDQFAKR
TH_1154|M.thermoresistible__bu VRTRWELEECLELVDAS---PLGR-QRGLRRWVMAEVPSVVHWLPEYVGM
Mflv_0749|M.gilvum_PYR-GCK IATAEEAKTF----------ADKARAHGLTPGVMIEVPAAALLAERILEH
Mvan_0097|M.vanbaalenii_PYR-1 IATAEEAKSF----------ADRARTHGLTPGVMIEVPAAALLADRILEH
MSMEG_2121|M.smegmatis_MC2_155 VTTLDELFAARRLLDDAVRRAGPRPPTGLQVGMMVEVPAAALKSAAFSRH
: * ** * *:* .
MMAR_3410|M.marinum_M -FDGFSIGSNDLTQLTLGVDRDSEIVAFDYDERDDGVKEMIRLAVEGCRR
TH_1154|M.thermoresistible__bu GIDGVSIGSNDLTQLMLGVDRDSDICAELFDESDAAVLDAIGRIVGTARR
Mflv_0749|M.gilvum_PYR-GCK -VEFLSIGTNDLAQYTMAADRMSAELATLTDPWQPAVLALVAATVKAGAA
Mvan_0097|M.vanbaalenii_PYR-1 -VDFLSIGTNDLAQYTMAADRMSAELATLTDPWQPAVLALVAMTVNAGAA
MSMEG_2121|M.smegmatis_MC2_155 -VDFLSIGTNDLSQYALAADRNNDAVAGLGDTFDPGLLHLIAATCRG-AA
.: .***:***:* :..** . * * : .: :
MMAR_3410|M.marinum_M NGIHSGLCGQAPSDYPDMAEFLVRAGIDSVSLNPDVVVKTTRQILDLEQQ
TH_1154|M.thermoresistible__bu LGITSSLCGQAPSTDPAFAEHLVRMGITSVSVNPDALESARRTVAAAERR
Mflv_0749|M.gilvum_PYR-GCK VGKPVGVCGEAAAD-PLLACVLTGFGVTSLSAAAAAVQGVGARLSAVTVQ
Mvan_0097|M.vanbaalenii_PYR-1 AGKPVGVCGEAAAD-PLLACVLTGFGVTSLSAAAAAVQAVGAKLAQVTLQ
MSMEG_2121|M.smegmatis_MC2_155 GAASVSVCGEFAAD-ARAVPLLIGLGVDALSVTPPAIPTTKEAVRAVDDR
. .:**: .: . . * *: ::* . .: . : :
MMAR_3410|M.marinum_M VVPLQ----------------------
TH_1154|M.thermoresistible__bu ILLESARNVQGDAPADAR---------
Mflv_0749|M.gilvum_PYR-GCK QCRDAAEAVLGTASAAEARAAALEVLG
Mvan_0097|M.vanbaalenii_PYR-1 QCRDAAEAVLRTASAADARAVAMSVLG
MSMEG_2121|M.smegmatis_MC2_155 HATETAEAALAAAGPAAVRDLLG----