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M. smegmatis MC2 155 MSMEG_0270 (-)

annotation: aminoglycoside phosphotransferase
coordinates: 304140 - 305144
length: 334

GVITDDVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAAL
RTDSDVTVPTVVPARDGRRVVTVDPADSDAVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHS
QRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHAD
LRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEA
MLPSFVFLRRLLLLAWMGSHTHSRESATKAISYAAGSCALAERYLSSDGLRLT*
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_0270--100% (334)aminoglycoside phosphotransferase
M. smegmatis MC2 155MSMEG_0780-6e-7243.60% (328) phosphotransferase enzyme family protein

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97-----
M. gilvum PYR-GCKMflv_1236-1e-13067.07% (331) aminoglycoside phosphotransferase
M. tuberculosis H37Rv-----
M. leprae Br4923-----
M. abscessus ATCC 19977MAB_0951-1e-6841.41% (326) putative aminoglycoside phosphotransferase
M. marinum M-----
M. avium 104-----
M. thermoresistible (build 8)TH_1675-1e-7243.38% (325) phosphotransferase enzyme family protein
M. ulcerans Agy99-----
M. vanbaalenii PYR-1Mvan_5572-1e-12967.17% (332) aminoglycoside phosphotransferase

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_1236|M.gilvum_PYR-GCK          MTAD-FTDDQTVAEKALAAFDLPQGSALRLLNLSENATYAVEEPGCGHRS
Mvan_5572|M.vanbaalenii_PYR-1       MSGDDVNDDRAVAEKALEAFDLPQGSTLRLLNLSENATYAVEEPGCGHRS
MSMEG_0270|M.smegmatis_MC2_155      --MGVITDDVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDG--EHQS
MAB_0951|M.abscessus_ATCC_1997      -MPGLPPNHSAFARAALTSYGRDPHTPLRLLSLSENATYLVDDQ---RPM
TH_1675|M.thermoresistible__bu      -MSGLPRDHERHARTVLPAYGRDPDTPLRLLSVSENAIYLVDDP---DPM
                                       .   :.   *. .*  :.    :.****.:**** * *::       

Mflv_1236|M.gilvum_PYR-GCK          ILRVHRKDYHRVDQIESELMWLDALRRDSDVTVPTVIPAQDGRRVVTVEH
Mvan_5572|M.vanbaalenii_PYR-1       ILRVHRQNYHRVDQIESELLWLDALRRDSDVTVPTVLPAHDGRRVVTVAH
MSMEG_0270|M.smegmatis_MC2_155      ILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDP
MAB_0951|M.abscessus_ATCC_1997      VLRAHRPGYHSLPAIRSELAWMSALRAETSVVTPELVRARNGSEVIAVGV
TH_1675|M.thermoresistible__bu      VLRVHRPGYHSPAAIRSELGWMTALREQTTVATPRALRTVDGRDVVAAQP
                                    :**.** .**    *.*** *: *** :: *..*  : : :*  *::.  

Mflv_1236|M.gilvum_PYR-GCK          DGDE---RYVVHFEMVPGAEPDEDTVTSTDFHTLGCITAALHDHARRWQR
Mvan_5572|M.vanbaalenii_PYR-1       DGAD---RHVVHFEMVPGAEPDENALTSTDFHTLGRITAALHDHARSWER
MSMEG_0270|M.smegmatis_MC2_155      ADSDAVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTR
MAB_0951|M.abscessus_ATCC_1997      DG---ITLHVDAMGFVPGCTAEE-TSGVVGYGKLGQLTAHMHHHAQGWRV
TH_1675|M.thermoresistible__bu      DGSRGSPVYVDAVTFIPGCTAEE-QPDAVGFEDLGRITAVMHRHVQSWSP
                                     .      :*  . :: *. .:*      .:  ** :** :* * : *  

Mflv_1236|M.gilvum_PYR-GCK          PSGFSRFAWDWEHSLGGTPRWGRWRDAVGVGEHEADVLTRAEALLHRRLT
Mvan_5572|M.vanbaalenii_PYR-1       PAAFSRFAWDWENSLGGSPRWGRWRDAIGVGAQEAEVLERAERLLHRRLA
MSMEG_0270|M.smegmatis_MC2_155      PAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLE
MAB_0951|M.abscessus_ATCC_1997      PPGFTRFRWDLDSMFGPQARWGDWRQAPGLTDADREAVDAAVRDIIRRLS
TH_1675|M.thermoresistible__bu      PAGFTRFRWDIDTTIGAHPRWGSWRHAAGLTPRDIDVVERAAAAVTARLT
                                    *..* ** ** :  :*  .*** *:.* *:   :   :  *   :  :* 

Mflv_1236|M.gilvum_PYR-GCK          DYGTGPDAFGLVHADLRLANLLVDDDT----ITVIDFDDCGMGWYFYDFG
Mvan_5572|M.vanbaalenii_PYR-1       EYGTGPDTFGLVHADLRLANLLVDGDT----ITVIDFDDCGFGWYFYDFG
MSMEG_0270|M.smegmatis_MC2_155      EYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFG
MAB_0951|M.abscessus_ATCC_1997      EYGCTPDRFGLIHADMRLSNLMVDPGRPGSDITVIDFDDCGWSWFMADLG
TH_1675|M.thermoresistible__bu      EFGYAPDKFGLIHGDLRMANLMIDPADPGAPITVIDFDDSGWSWYLADLG
                                    ::*  ** :**:*.*:*::**::*       ********.* .*:: *:*

Mflv_1236|M.gilvum_PYR-GCK          TAVSFFEDHPSVPEWQDAWVSGYRSRRPISAADEDMLASFILLRRLLLLA
Mvan_5572|M.vanbaalenii_PYR-1       TAVSFFEDHPSVPEWQEAWVSGYRTRRTLAASDEDMLASFILLRRLLLLA
MSMEG_0270|M.smegmatis_MC2_155      TAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLA
MAB_0951|M.abscessus_ATCC_1997      AVVSWVEDTPMAEAAVADWLDGYQRVRRLSGDDLGMIPAFVMLRRVMLTA
TH_1675|M.thermoresistible__bu      AVVSFIEHTPLAESIIADWLCGYREVCELPQADLEMVPTFVMLRRLMLTG
                                    :.**:.*. *        *: **:    :.  *  *:.:*::***::* .

Mflv_1236|M.gilvum_PYR-GCK          WMGTHSHSKESQAISVTYAAGSCTLAEHYLSSD--------GHRIF
Mvan_5572|M.vanbaalenii_PYR-1       WMGTHSHSKESQAISVTYAAGSCKLAEQYLSSD--------GHRLF
MSMEG_0270|M.smegmatis_MC2_155      WMGSHTHSRESATKAISYAAGSCALAERYLSSD--------GLRLT
MAB_0951|M.abscessus_ATCC_1997      WIASHADADAAIRVSDGFGARTARLAERYLTDRTWLREAVFGRRVG
TH_1675|M.thermoresistible__bu      WIASHPDADAAITYRDGYPEGTVQLAHRYLGDRNWLRDAVFSTRV-
                                    *:.:*..:  :      :   :  **.:** .         . *: