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MPLAAGSTFAGYTIVRQLGTGGMGEVYLAQHPRLPRQDALKVLRPEVSADHEYRQRFHREADAAAALWHP HIVAVHDRGETDGQLWIDMDYVDGTDAVTLLRDRYPNGMPGSEVSEIITAVAEALDYAHDHKLLHRDVKP ANILIAAPASQAKRIMLADFGIAGWVGEASGLTATNMTVGTVSYAPPEQLMGHDLDGKADQYALAATAYH LLTGSPPFQHSNPAVVISQHLSAAPPAIGQRLPALAELDPVFAKALAKDPKDRYQRCIDFARALSHRLGG VGDPDDTRISRPVAVAVPAAKKSLARASVIVPAVLAILLVVAIAVALREFERADDERVAPPAATSSSRPS PTAAKTSTTTATTTAPTSTSTSTAAATTESVTPAPAVVIGAMCSPLGSTGTTKTGATAYCSTLQGTNTTI WSLTEGTVASPTVSATPDPTEPPLPIDQDSPIRICMQQTGQTRHECREDIRRSNGY
Operon Prediction Model: Genebank
Paralogs
species | id | gene | e-value | identity (len) | annotation |
M. marinum M | MMAR_2606 | pknF_3 | - | 100% (476) | anchored-membrane serine/threonine-protein kinase PknF_3 |
M. marinum M | MMAR_4174 | pknF | e-113 | 55.19% (395) | anchored-membrane serine/threonine-protein kinase PknF |
M. marinum M | MMAR_2408 | pknF_1 | e-105 | 54.96% (393) | anchored-membrane serine/threonine-protein kinase PknF_1 |
Closest Orthologs (e-value cutoff: 1e-4)
species | id | gene | e-value | identity (len) | annotation |
M. bovis AF2122 / 97 | Mb1775 | pknF | 0.0 | 78.62% (477) | anchored-membrane serine/threonine-protein kinase PKNF |
M. gilvum PYR-GCK | Mflv_3427 | - | 1e-157 | 61.89% (475) | protein kinase |
M. tuberculosis H37Rv | Rv1746 | pknF | 0.0 | 78.83% (477) | anchored-membrane serine/threonine-protein kinase PKNF |
M. leprae Br4923 | MLBr_00016 | pknB | 4e-49 | 35.40% (404) | putative serine/threonine protein kinase |
M. abscessus ATCC 19977 | MAB_4435 | - | 3e-64 | 39.49% (390) | serine/threonine-protein kinase PknJ |
M. avium 104 | MAV_3145 | - | 1e-103 | 54.37% (366) | PknF protein |
M. smegmatis MC2 155 | MSMEG_3677 | - | 1e-174 | 63.51% (496) | serine/threonine protein kinase |
M. thermoresistible (build 8) | TH_1607 | pknI | 3e-91 | 71.68% (226) | PROBABLE TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE I |
M. ulcerans Agy99 | MUL_4471 | pknF | 1e-112 | 54.94% (395) | anchored-membrane serine/threonine-protein kinase PknF |
M. vanbaalenii PYR-1 | Mvan_5635 | - | 1e-133 | 53.31% (484) | protein kinase |
CLUSTAL 2.0.9 multiple sequence alignment Mb1775|M.bovis_AF2122/97 -------------------------------------------------- Rv1746|M.tuberculosis_H37Rv -------------------------------------------------- MMAR_2606|M.marinum_M -------------------------------------------------- MSMEG_3677|M.smegmatis_MC2_155 -------------------------------------------------- Mflv_3427|M.gilvum_PYR-GCK -------------------------------------------------- TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 -------------------------------------------------- MAV_3145|M.avium_104 MNEHKGTTRRIGAGRRLLSNPRQARAALRAGFQSLPGMPIGQLFRRIPPV MUL_4471|M.ulcerans_Agy99 -------------------------------------------------- MAB_4435|M.abscessus_ATCC_1997 -------------------------------------------------- MLBr_00016|M.leprae_Br4923 -------------------------------------------------- Mb1775|M.bovis_AF2122/97 ---------------------MPLAEGSTFAGFTIVRQLGSGGMGEVYLA Rv1746|M.tuberculosis_H37Rv ---------------------MPLAEGSTFAGFTIVRQLGSGGMGEVYLA MMAR_2606|M.marinum_M ---------------------MPLAAGSTFAGYTIVRQLGTGGMGEVYLA MSMEG_3677|M.smegmatis_MC2_155 ---------------------MPLAAGETFAGYNIIRLLGSGGMGEVYLA Mflv_3427|M.gilvum_PYR-GCK ---------------------MPLAAGETFAGYTIVRLLGSGGMGEVYLA TH_1607|M.thermoresistible__bu ---------------------MPLADGQQFAEYTILRRLGAGGMGEVYLA Mvan_5635|M.vanbaalenii_PYR-1 ---------------------MPLSDGEVIAGYTIVRMLGSGGMGKVYLA MAV_3145|M.avium_104 DSRPHPEPAAAQGNSEPGGPRLTIGNGASFAGYTILRQLGAGGMAEVYLA MUL_4471|M.ulcerans_Agy99 ---------------------MPLPPGSAFAGYTIVGLLGSGAMGEVYLA MAB_4435|M.abscessus_ATCC_1997 -----MTSSDRSSPGPGGQQPGDLPPGTVVSGYQIERVLGRGGMGTVYLA MLBr_00016|M.leprae_Br4923 ----------------------MTTPPHLSDRYELGDILGFGGMSEVHLA : : ** *.*. *:** Mb1775|M.bovis_AF2122/97 RHPRLPRQDALKVLRADVSADGEYRARFNREADAAASLWHPHIVAVHDRG Rv1746|M.tuberculosis_H37Rv RHPRLPRQDALKVLRADVSADGEYRARFNREADAAASLWHPHIVAVHDRG MMAR_2606|M.marinum_M QHPRLPRQDALKVLRPEVSADHEYRQRFHREADAAAALWHPHIVAVHDRG MSMEG_3677|M.smegmatis_MC2_155 AHPRLPRQDALKVLPTSISADPEYRERFNREADVAAALWHPHIVEIHDRG Mflv_3427|M.gilvum_PYR-GCK QHPRLPRRDALKVLPAAVSADVEYRKRFEREADIAATLWHPHIVGVHDRG TH_1607|M.thermoresistible__bu RHPRLPRNDALKVLSAEVSADDEYRRRFEQEADIAATLWHPHIVAVHDRG Mvan_5635|M.vanbaalenii_PYR-1 KHPRLPRYDALKVLSTTVCADSEYRERFHREADIAATLWHPHIVAVHDRG MAV_3145|M.avium_104 LHPRLPRRDVIKVLAEAVTVDPEFRERFNREADLAATLWHPHIVGVHDRG MUL_4471|M.ulcerans_Agy99 QHPRLPRRDALKVLPPDVSADTAFRERFQREADLAATLLHPHIVGVHDRG MAB_4435|M.abscessus_ATCC_1997 ANPNLQRSEALKILSAQSSRDPEFRARFMREASLAASLDHPNIVTVHNRG MLBr_00016|M.leprae_Br4923 RDIRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPSIVAVYDTG . .* * .:*:* * : ** :**. **:* ** ** ::: * Mb1775|M.bovis_AF2122/97 EF----DGQLWIDMDFVDGTDTVSLLRDRYPNGMPGPEVTEIITAVAEAL Rv1746|M.tuberculosis_H37Rv EF----DGQLWIDMDFVDGTDTVSLLRDRYPNGMPGPEVTEIITAVAEAL MMAR_2606|M.marinum_M ET----DGQLWIDMDYVDGTDAVTLLRDRYPNGMPGSEVSEIITAVAEAL MSMEG_3677|M.smegmatis_MC2_155 EH----DGQLWIAMDYVDGTDAARLLKEQYSGGMPPHQVCEIVTAMAGAL Mflv_3427|M.gilvum_PYR-GCK ED----AGRIWISMDYVEGADAAHLLAEDYRTGLPVAEVAQIVTAVADAL TH_1607|M.thermoresistible__bu EY----DGQLWIAMDHVDGTDVGRLLGEQYPNGMPPDAVIRIITAVADAL Mvan_5635|M.vanbaalenii_PYR-1 EY----EGRLWISMDHVEGTDAAWLLAERYPHGMPPALVIRIVTAVGEAL MAV_3145|M.avium_104 EF----NGHLWISMDYVEGTDASRLVKESYADGMPLDEVSAIVQAVAGAL MUL_4471|M.ulcerans_Agy99 EF----NGQLWISMDYIEGTDAAALIRSQYPAGMPVAEVVAVVTAVAGAL MAB_4435|M.abscessus_ATCC_1997 ETP---EGDLWIAMQYVQGSDAH---SESASGRMSLTRAVHIISEVAKAL MLBr_00016|M.leprae_Br4923 EAETSAGPLPYIVMEYVDGATLRDIVHTDGP--MPPQQAIEIVADACQAL * :* *:.::*: :. . :: ** Mb1775|M.bovis_AF2122/97 DYAHERRLLHRDVKPANILIANPDSPD--RRIMLADFGIAGWVDDPSGLT Rv1746|M.tuberculosis_H37Rv DYAHERRLLHRDVKPANILIANPDSPD--RRIMLADFGIAGWVDDPSGLT MMAR_2606|M.marinum_M DYAHDHKLLHRDVKPANILIAAPASQA--KRIMLADFGIAGWVGEASGLT MSMEG_3677|M.smegmatis_MC2_155 DYAHQRGLLHRDVKPANILLGHPGTDD--QRIMLADFGIARYADEASGLT Mflv_3427|M.gilvum_PYR-GCK DYAHDRHLLHRDVKPANILIARPDSNT--RRIMLADFGIARWDNDISGLT TH_1607|M.thermoresistible__bu DHAHSRNLLHRDVKPANILLAAGDGEQGAERVLLADFGIARWIDRSSGLT Mvan_5635|M.vanbaalenii_PYR-1 DYAHQRGLLHRDVKPANILIADPETEN--ERIMLADFGIARRVGEVSTLT MAV_3145|M.avium_104 DYAHARGLLHRDVKPANILLTHP-EAGE-RRILLADFGVARHLGDISGIT MUL_4471|M.ulcerans_Agy99 DYAHQRGLLHRDVKPANILLTGPDNAGG-PRIFLADFGIARQLDDISGIT MAB_4435|M.abscessus_ATCC_1997 DYLHARGLVHRDVKPANILLSAGD-----RRIMLGDFGLTRALDDSNSVT MLBr_00016|M.leprae_Br4923 NFSHQNGIIHRDVKPANIMISATN------AVKVMDFGIARAIADSTSVT :. * . ::*********:: : : ***:: . :* Mb1775|M.bovis_AF2122/97 ATNMTVGTVSYAAPEQLMGNE-LDGRADQYALAATAFHLLTGSPPFQHAN Rv1746|M.tuberculosis_H37Rv ATNMTVGTVSYAAPEQLMGNE-LDGRADQYALAATAFHLLTGSPPFQHAN MMAR_2606|M.marinum_M ATNMTVGTVSYAPPEQLMGHD-LDGKADQYALAATAYHLLTGSPPFQHSN MSMEG_3677|M.smegmatis_MC2_155 ATNMTVGTVTYAAPEQLLGNH-LDGRADQYALAATAYQLLTGRTPFEHSN Mflv_3427|M.gilvum_PYR-GCK ATNMTVGTVSYAAPEQLMGQD-LDGRADQYALAATAFHLLTGSPPFSHSN TH_1607|M.thermoresistible__bu ETDMTVGTVAYAAPEQLRAAD-IDGRADQYALAATAFQLMTGAPPFQHSN Mvan_5635|M.vanbaalenii_PYR-1 GTSMTVGTVAYSAPEQLTADEHIDGRADQYALAATAFQLLTGSPPFQHSN MAV_3145|M.avium_104 ETNVAVGTVAYAAPEQLTGSP-IDGRADQYALAATAFHLLTGAPPFQHSN MUL_4471|M.ulcerans_Agy99 ATNLTVGTAAYAAPEQLTGAD-VDGRADQYALAATAFHLLTGAPPYQHAN MAB_4435|M.abscessus_ATCC_1997 SAGNVTATIAYAAPEILSGTA-VDGRADVYALGCTLFRMLTGQPPFGSGN MLBr_00016|M.leprae_Br4923 QTAAVIGTAQYLSPEQARGDS-VDARSDVYSLGCVLYEILTGEPPFIGDS : . .* * .** . :*.::* *:*... :.::** .*: . Mb1775|M.bovis_AF2122/97 -PAVVISQHLSASPPAIGDRVP-ELTPLDPVFAKALAKQPKDRYQRCVDF Rv1746|M.tuberculosis_H37Rv -PAVVISQHLSASPPAIGDRVP-ELTPLDPVFAKALAKQPKDRYQRCVDF MMAR_2606|M.marinum_M -PAVVISQHLSAAPPAIGQRLP-ALAELDPVFAKALAKDPKDRYQRCIDF MSMEG_3677|M.smegmatis_MC2_155 -PAVVISQHLSAAPPAVGTHRP-DLATLDPVFAKALAKDPRDRYARCADF Mflv_3427|M.gilvum_PYR-GCK -PAVVISRHLNSAPATVAAHRP-ELAAVDPVLTRALAKNPADRYPRCADF TH_1607|M.thermoresistible__bu -PAXXXCGR----------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 -PAIVISQHLTAQPPSISVHRP-ELSSLGMAFEKALAKSPADRFDRCVDF MAV_3145|M.avium_104 -PIAVIGQHLHEDPPRLSDFRP-ELAALDEVFCQALAKVPEDRFDRCRAF MUL_4471|M.ulcerans_Agy99 -SVAVISQHLTAPPPALGDRRS-ELAPLDRVFAAALAKQPNERYWRCADF MAB_4435|M.abscessus_ATCC_1997 SLPAIINAHLSAPPPRISQLAP-VPESWDALIAKAMAKTPADRYQSAGEL MLBr_00016|M.leprae_Br4923 -PVSVAYQHVREDPIPPSQRHEGISVDLDAVVLKALAKNPENRYQTAAEM : Mb1775|M.bovis_AF2122/97 ARALG------------------HRLGGAGDPDDTRVSQPVAVAAP-AKR Rv1746|M.tuberculosis_H37Rv ARALG------------------HRLGGAGDPDDTRVSQPVAVAAP-AKR MMAR_2606|M.marinum_M ARALS------------------HRLGGVGDPDDTRISRPVAVAVPAAKK MSMEG_3677|M.smegmatis_MC2_155 ARALR------------------HRLGTDADVADPDATRLAPVARHAAPH Mflv_3427|M.gilvum_PYR-GCK AREFN------------------ARLDAGAP----------------AHT TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 ARALA------------------NRSGTELPGSAFDPDATRHAAAPTGPR MAV_3145|M.avium_104 AAAVSRECDGAAAI------GPDARSRTVASPAHRRRGPGRVIAAVTHRF MUL_4471|M.ulcerans_Agy99 AQALS----------------------QVASPQG-------AAAAVS--- MAB_4435|M.abscessus_ATCC_1997 AMAAQQILTDPAQHRQTVTQPVPATTPAPIAAPEPVHAMAPLAMADTGQS MLBr_00016|M.leprae_Br4923 RADLIRVHSGQPPEAPKVLTDADRSCLLSSGAGNFGVPRTDALSRQSLDE Mb1775|M.bovis_AF2122/97 SLLRTAVIVPAVLAMLLVMAVAVTVREFQRADDERAAQPARTRTTTSAGT Rv1746|M.tuberculosis_H37Rv SLLRTAVIVPAVLAMLLVMAVAVAVREFQRADDERAAQPARTRTTTSAGT MMAR_2606|M.marinum_M SLARASVIVPAVLAILLVVAIAVALREFERADDERVAPPAATSSSRPSPT MSMEG_3677|M.smegmatis_MC2_155 KRPKTAALIAGVLGVLLVAAIVVAAVEFRRADDERPVARASGAATTTSHT Mflv_3427|M.gilvum_PYR-GCK SRIRPAVLIPVVLAVLLVAAVAVAAFEFLRADR---ADRADRADPPPMAR TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 HAKTAAAPRSRRRVVLAGAGLVLALIAVA-------AAVFLARFERDVGR MAV_3145|M.avium_104 SPQTRWAAALVCAVLVAVAATWSVLYSFQPGAPPANPALASKPSPPAAVA MUL_4471|M.ulcerans_Agy99 -PSDPTLPVAICVPPEYAAATQAAPAPMP--WYPTAPAPARPRRQVRTIV MAB_4435|M.abscessus_ATCC_1997 MLKHKRIAAIIGVMVLVVASSVVAVLIWPRADEGTDPRTKASPSAAQVAP MLBr_00016|M.leprae_Br4923 TESDGSIGRWVAVVAVLAVLTIAIVAAFNTFGGNTRDVQVPDVRGQVSAD Mb1775|M.bovis_AF2122/97 TTSVAPASTTRP-------------------------------------- Rv1746|M.tuberculosis_H37Rv TTSVAPASTTRP-------------------------------------- MMAR_2606|M.marinum_M AAKTSTTTATTT-------------------------------------- MSMEG_3677|M.smegmatis_MC2_155 TTPVTTAPASPPPEVTDTVTIT---------------------------- Mflv_3427|M.gilvum_PYR-GCK TTTITTSPTTST-------------------------------------- TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 GEARATASATAS-------------------------------------- MAV_3145|M.avium_104 APIAGGPVLNGTYKLDYDQTKRTTNGIGIRHDGAGTNWWA-----FRSAC MUL_4471|M.ulcerans_Agy99 VPVVVALLLVSAVAFAVTQVLR---------------------------- MAB_4435|M.abscessus_ATCC_1997 GASLAGQPNTVLDTLLPGQFDYPEG------------------------- MLBr_00016|M.leprae_Br4923 AISALQNRGFKTRTLQKPDSTIPPDHVISTEPGANASVGAGDEITINVST Mb1775|M.bovis_AF2122/97 ---------------------APTTPTTTGAADTATASP----------- Rv1746|M.tuberculosis_H37Rv ---------------------APTTPTTTGAADTATASP----------- MMAR_2606|M.marinum_M ---------------------APTSTSTSTAAATTESVT----------- MSMEG_3677|M.smegmatis_MC2_155 -------------DTATDTAAVDTSTPATTTAATTAAAA----------- Mflv_3427|M.gilvum_PYR-GCK --------------------------------TTATPPA----------- TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 ------------------------------TAPTGSGSV----------- MAV_3145|M.avium_104 TSSGCAATGTRLDDATHQAAGGPDGDQTDTLRFVGGYWQGAPEQQRVGCT MUL_4471|M.ulcerans_Agy99 ----------------------PDPRQTTAAPAEAPQWQPYVDH------ MAB_4435|M.abscessus_ATCC_1997 ----------WEKNPSPTFRSGEFNPAVPPGSATNIGGTPVGCNTIDPAW MLBr_00016|M.leprae_Br4923 GPEQREVPDVSSLNYTDAVKKLTSSGFKSFKQANSPSTPELLGKVIGTNP Mb1775|M.bovis_AF2122/97 -------TAAVVAIGALCFPLG--------------------------ST Rv1746|M.tuberculosis_H37Rv -------TAAVVAIGALCFPLG--------------------------ST MMAR_2606|M.marinum_M -------PAPAVVIGAMCSPLG--------------------------ST MSMEG_3677|M.smegmatis_MC2_155 -------AAQTAVIGASCSPVG--------------------------AT Mflv_3427|M.gilvum_PYR-GCK -------TPASVVIGANCAPLD--------------------------AT TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 -------TLPVVVVGADCATLG--------------------------AA MAV_3145|M.avium_104 RPGGPAGATQQETIARSLAPQSDGTLRGTETETVLSNECGAQGAVVRVPV MUL_4471|M.ulcerans_Agy99 -------AKQFAISLTSVNPQT-------------------------IDA MAB_4435|M.abscessus_ATCC_1997 ALRAKGRETSTLYLRDRKKPEREILIR-----------------VVPAAD MLBr_00016|M.leprae_Br4923 SANQTSAITNVITIIVGSGPETKQIPDVTG---------------QIVEI Mb1775|M.bovis_AF2122/97 GTTKTGATAYCSTLQGTNTTIWSLTEDTVASPTVTATADPTEAPLPIEQE Rv1746|M.tuberculosis_H37Rv GTTKTGATAYCSTLQGTNTTIWSLTEDTVASPTVTATADPTEAPLPIEQE MMAR_2606|M.marinum_M GTTKTGATAYCSTLQGTNTTIWSLTEGTVASPTVSATPDPTEPPLPIDQD MSMEG_3677|M.smegmatis_MC2_155 ATTSDGSTAYCSTLATTGASIWSLTQGTIPSPTV--TPAATDEPLPFAEE Mflv_3427|M.gilvum_PYR-GCK ATTGDGTTAYCAAVPGSAGTIWSLTQGTLPAPTSTPPPDPTEQPLSDAEA TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 GVTEAGDPAYCARLASSGAPLWSLYPGEIPHPTGTSAPEGESQ----GPD MAV_3145|M.avium_104 VATRVGDVPPGVTVADPASVINASPTATAPAPPVLGGLCSDVGKVAYDPT MUL_4471|M.ulcerans_Agy99 DIQRILDGSTGAFHSDFADKSAQFKQSIVDAGSATQGKATGAGLESISGT MAB_4435|M.abscessus_ATCC_1997 ALSTDGASDALKRCDAVAYIIPGSSTTWRCSFEFDEPNPVQNGQKELTTT MLBr_00016|M.leprae_Br4923 AQKNLNVYGFTKFSQASVDSPRPTGEVIGTNPPKDATVPVDSVIELQVSK Mb1775|M.bovis_AF2122/97 SPIRVCMQQTGQTRRECREEIRRS------------------NGWP---- Rv1746|M.tuberculosis_H37Rv SPIRVCMQQTGQTRRECREEIRRS------------------NGWP---- MMAR_2606|M.marinum_M SPIRICMQQTGQTRHECREDIRRS------------------NGY----- MSMEG_3677|M.smegmatis_MC2_155 SPVRVCMQQTGMTRRECREAIRRS------------------NGLP---- Mflv_3427|M.gilvum_PYR-GCK FPVLTCMEQTGQTRRECRRAIRES------------------NGQPPLP- TH_1607|M.thermoresistible__bu -------------------------------------------------- Mvan_5635|M.vanbaalenii_PYR-1 TPVLVCMQQTGQSQVDCHDDILQE------------------NTDPGAND MAV_3145|M.avium_104 NNEQIVCEGSSWAKAPITMGVHAAGSSCDRPGTSVFAMSTSSDGYLLQCD MUL_4471|M.ulcerans_Agy99 TAHVVVSVTSNITKS---TGVQQE----PRQWRLRIQIEKIDDAYLASRV MAB_4435|M.abscessus_ATCC_1997 ESCYMFPSGANSIRQHVSFNVVRGLLVYILASGASNRNDAAGSLSATALS MLBr_00016|M.leprae_Br4923 GNQFVMPDLSGMFWADAEPRLRALGWTGVLDKGPDVDAGGSQHNRVAYQN Mb1775|M.bovis_AF2122/97 ------------------- Rv1746|M.tuberculosis_H37Rv ------------------- MMAR_2606|M.marinum_M ------------------- MSMEG_3677|M.smegmatis_MC2_155 ------------------- Mflv_3427|M.gilvum_PYR-GCK ------------------- TH_1607|M.thermoresistible__bu ------------------- Mvan_5635|M.vanbaalenii_PYR-1 TQTPG-------------- MAV_3145|M.avium_104 PVTRTWTRPAG-------- MUL_4471|M.ulcerans_Agy99 EFVT--------------- MAB_4435|M.abscessus_ATCC_1997 FATTMKILRYGR------- MLBr_00016|M.leprae_Br4923 PPAGAGVNRDGIITLKFGQ