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TIGLGIPAPPAPRLAPRRVTRQLMVGNVGIGSDYPVAVQSMTTTKTHDVNATLQQIAELTAAGCNIVRVA CPRQEDADALPQIVKHSHIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDSRVKEVAKAAADTGIP IRIGVNAGSLGKRIIQRYGKATPEALVESALWEASLFEEHGFGDIKISVKHHDPVVMVAAYQLLATQCDY PLHLGVTEAGPAFQGTIKSAVAFGALLSSGIGDTIRVSLSAPPIEEVKVGTQILESLNLRPRSLEIVSCP SCGRAQVDVYRLANEVSAGLDGLGAPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGEVIKTVPE SQIVETLIEQALRIAEERASETECTDLVGTPVVSEV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_0275 | ispG | - | 100% (387) | GcpE protein |
| M. marinum M | MMAR_1838 | ispG | 0.0 | 85.71% (385) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, IspG |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2893c | ispG | 0.0 | 84.16% (385) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. gilvum PYR-GCK | Mflv_4081 | ispG | 0.0 | 84.07% (383) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. tuberculosis H37Rv | Rv2868c | ispG | 0.0 | 84.16% (385) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. leprae Br4923 | MLBr_01581 | ispG | 0.0 | 82.38% (386) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. abscessus ATCC 19977 | MAB_3169c | ispG | 0.0 | 85.07% (375) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. avium 104 | MAV_3725 | ispG | 1e-175 | 86.50% (363) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. smegmatis MC2 155 | MSMEG_2580 | ispG | 0.0 | 85.42% (384) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. thermoresistible (build 8) | TH_1399 | gcpE | 0.0 | 87.17% (374) | PROBABLE GCPE PROTEIN |
| M. ulcerans Agy99 | MUL_2087 | ispG | 1e-177 | 85.87% (361) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
| M. vanbaalenii PYR-1 | Mvan_2262 | ispG | 0.0 | 87.74% (367) | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2893c|M.bovis_AF2122/97 MTV----GLGMPQPP----APTLAPRRATRQLMVGNVGVGSDHPVSVQSM
Rv2868c|M.tuberculosis_H37Rv MTV----GLGMPQPP----APTLAPRRATRQLMVGNVGVGSDHPVSVQSM
MUL_2087|M.ulcerans_Agy99 --------------------------------MVRDVGVGSDYPVSVQSM
MLBr_01581|M.leprae_Br4923 MNM----RLGMPEAP----APGLAPRRITRQLMVGNVGVGSDHQISVQSM
MAV_3725|M.avium_104 --------------------------------MVRDVGVGSDYPISVQSM
Mflv_4081|M.gilvum_PYR-GCK MTSTAGVGLGMPAPP----PPVLAPRRKTRQLMVGDVGVGSDHPIAVQSM
Mvan_2262|M.vanbaalenii_PYR-1 MTS---IGLGIPAPP----APVLAPRRKTRQLMVRDVGVGSDHPISVQSM
MSMEG_2580|M.smegmatis_MC2_155 MTS---IGLGMPAPP----APTLAPRRKTRQLNVGGVGIGSEHPIAVQSM
MAB_3169c|M.abscessus_ATCC_199 MMT----GLGMPSLPGA-PPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSM
TH_1399|M.thermoresistible__bu MTS---IGLGMPAVGSGGPAPVLAPRRKTRQLMVCDVGVGSDHPISVQSM
MMAR_0275|M.marinum_M MTI----GLGIPAPP----APRLAPRRVTRQLMVGNVGIGSDYPVAVQSM
* .**:**: ::****
Mb2893c|M.bovis_AF2122/97 CTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADALAEIARHSQIPV
Rv2868c|M.tuberculosis_H37Rv CTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADALAEIARHSQIPV
MUL_2087|M.ulcerans_Agy99 CTTKTHDVNATLQQIAELTAAGCDIVRVACPRQEDADALAEIARHSQIPV
MLBr_01581|M.leprae_Br4923 CITKTHDVNATLQQIAELTTAGCDIVRVACPRQEDADALAEIARHSKIPV
MAV_3725|M.avium_104 CTTKTHDVNSTLQQIAELTAAGCDIVRVACPRQEDADALAEIARHSQIPV
Mflv_4081|M.gilvum_PYR-GCK CTTKTHDINATLQQIAELTASGCDIVRVACPRQEDADALPAIAKKANIPV
Mvan_2262|M.vanbaalenii_PYR-1 CTTKTHDINATLQQIAELTASGCDIVRVACPRQEDADALPIIAKKSKIPV
MSMEG_2580|M.smegmatis_MC2_155 CTTKTHDVNSTLQQIAELTASGCDIVRVACPRQEDADALAEIARHSQIPV
MAB_3169c|M.abscessus_ATCC_199 CTTKTHDVNSTLQQIAELTASGCDIVRVACPRQEDADALAEIARKSKIPV
TH_1399|M.thermoresistible__bu CTTKTHDVNATLQQIAELTAAGCDIVRVACPRQEDADALPAIAAKSKIPV
MMAR_0275|M.marinum_M TTTKTHDVNATLQQIAELTAAGCNIVRVACPRQEDADALPQIVKHSHIPV
*****:*:*********::**:***************. *. :::***
Mb2893c|M.bovis_AF2122/97 VADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPI
Rv2868c|M.tuberculosis_H37Rv VADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPI
MUL_2087|M.ulcerans_Agy99 AADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAGAAGIPI
MLBr_01581|M.leprae_Br4923 IVDIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAAAAGIPI
MAV_3725|M.avium_104 IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKAAAAAGIPI
Mflv_4081|M.gilvum_PYR-GCK IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPI
Mvan_2262|M.vanbaalenii_PYR-1 IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVAEVAKAAGDAGIPI
MSMEG_2580|M.smegmatis_MC2_155 IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPI
MAB_3169c|M.abscessus_ATCC_199 IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPI
TH_1399|M.thermoresistible__bu IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPI
MMAR_0275|M.marinum_M IADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDSRVKEVAKAAADTGIPI
.******:************************.** ******. :****
Mb2893c|M.bovis_AF2122/97 RIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEEHGFGDIKISVKH
Rv2868c|M.tuberculosis_H37Rv RIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEEHGFGDIKISVKH
MUL_2087|M.ulcerans_Agy99 RIGVNAGSLDKRFMQKYGKATPEALVESALWEASLFEEHGFGNIKISVKH
MLBr_01581|M.leprae_Br4923 RIGVNAGSLDKRFMDKYGKATSEALVESALWEASLFEEHGFGNIKISVKH
MAV_3725|M.avium_104 RIGVNAGSLDKRFMAKYGKATPEALVESALWEASLFEEHGFSDIKISVKH
Mflv_4081|M.gilvum_PYR-GCK RIGVNAGSLDPRILKKYGKATPEALVESAMWEASLFEEHGFGDIKISVKH
Mvan_2262|M.vanbaalenii_PYR-1 RIGVNAGSLDKRFLEKYGKATPEALVESALWEASLFEEHGFGNIKISVKH
MSMEG_2580|M.smegmatis_MC2_155 RIGVNAGSLDKRFLEKYGKATPEALVESALWEASLFEEHGFGDIKISVKH
MAB_3169c|M.abscessus_ATCC_199 RIGVNAGSLDPRILGKYGKATPEALVESALWEAGLFEEHGFGDIKISVKH
TH_1399|M.thermoresistible__bu RIGVNAGSLDKRFMQKYGKATPEALVESALWEASLFEEHGFGDIKISVKH
MMAR_0275|M.marinum_M RIGVNAGSLGKRIIQRYGKATPEALVESALWEASLFEEHGFGDIKISVKH
*********. *:: :*****.*******:***.*******.:*******
Mb2893c|M.bovis_AF2122/97 NDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGI
Rv2868c|M.tuberculosis_H37Rv NDPVVMVAAYELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGI
MUL_2087|M.ulcerans_Agy99 NDPVIMVAAYEQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGI
MLBr_01581|M.leprae_Br4923 HDPVVMVAAYEQLAAQCDYPLHLGVTEAGPVFQGTVKSAVAFGALLSKGI
MAV_3725|M.avium_104 NDPVVMVAAYEQLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGI
Mflv_4081|M.gilvum_PYR-GCK NDPVIMVAAYEQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSKGI
Mvan_2262|M.vanbaalenii_PYR-1 NDPVVMVAAYEQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSKGI
MSMEG_2580|M.smegmatis_MC2_155 NDPVIMVAAYEQLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSKGI
MAB_3169c|M.abscessus_ATCC_199 NDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSKGI
TH_1399|M.thermoresistible__bu SDPVVMVQAYEMLAEQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGI
MMAR_0275|M.marinum_M HDPVVMVAAYQLLATQCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSSGI
***:** **: ** :**************.****:*********** **
Mb2893c|M.bovis_AF2122/97 GDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYT
Rv2868c|M.tuberculosis_H37Rv GDTIRVSLSAPPVEEVKVGNQVLESLNLRPRSLEIVSCPSCGRAQVDVYT
MUL_2087|M.ulcerans_Agy99 GDTIRVSLSAPPVEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYT
MLBr_01581|M.leprae_Br4923 GDTIRVSLSAPPVEEIKVGNQILESLNLRPRSHEIVSCPSCGRAQVDVYK
MAV_3725|M.avium_104 GDTIRVSLSAPPVEEVKVGIQILESLNLRPRSLEIVSCPSCGRAQVDVYT
Mflv_4081|M.gilvum_PYR-GCK GDTIRVSLSAPPAEEVKVGNQILESLNLRPRGLEIVSCPSCGRAQVDVYT
Mvan_2262|M.vanbaalenii_PYR-1 GDTIRVSLSAPPAEEVKVGNQILESLNLRPRGLEIVSCPSCGRAQVDVYT
MSMEG_2580|M.smegmatis_MC2_155 GDTIRVSLSAPPAEEVKVGNQILESLNLRPRGLEIVSCPSCGRAQVDVYT
MAB_3169c|M.abscessus_ATCC_199 GDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYT
TH_1399|M.thermoresistible__bu GDTIRVSLSAPPVEEVKVGVQILESLNLRPRGLEIVSCPSCGRAQVDVYT
MMAR_0275|M.marinum_M GDTIRVSLSAPPIEEVKVGTQILESLNLRPRSLEIVSCPSCGRAQVDVYR
************ **:*** *:*********. ****************
Mb2893c|M.bovis_AF2122/97 LANEVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVR
Rv2868c|M.tuberculosis_H37Rv LANEVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVR
MUL_2087|M.ulcerans_Agy99 LANEVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVR
MLBr_01581|M.leprae_Br4923 LANEVSAGLDGLEVPLRIAVMGCVVNGPGEARDADLGVASGNGKGQIFVK
MAV_3725|M.avium_104 LANEVSAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVR
Mflv_4081|M.gilvum_PYR-GCK LANEVTAGLEGLEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
Mvan_2262|M.vanbaalenii_PYR-1 LANEVTAGLEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
MSMEG_2580|M.smegmatis_MC2_155 LANAVTAGLDGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
MAB_3169c|M.abscessus_ATCC_199 LANAVSAGLEGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
TH_1399|M.thermoresistible__bu LANEVTAGLEGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
MMAR_0275|M.marinum_M LANEVSAGLDGLGAPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVK
*** *:***:*: .***:**************:****************:
Mb2893c|M.bovis_AF2122/97 GEVIKTVPEAQIVETLIEEAMRLAAEMGEQAPG-----ATPSGSPIVTVS
Rv2868c|M.tuberculosis_H37Rv GEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPG-----ATPSGSPIVTVS
MUL_2087|M.ulcerans_Agy99 GEVIKTVPEAQIVETLIEEAMRIAAEMGTEDPG-----TTSSGAPIVTVS
MLBr_01581|M.leprae_Br4923 GEVIKTVPEAQIVETLIEEAMRLVSEMGTEKGSDHCSETTRSGSPVVTVN
MAV_3725|M.avium_104 GEVIKTVPESQIVETLIEEAMRIAAQMNSEGGP----EATSSGSPVVTVS
Mflv_4081|M.gilvum_PYR-GCK GEVIKTVPEALIVETLIEEAMRLAADMDTTP----GSEEA-SGSPVVTVS
Mvan_2262|M.vanbaalenii_PYR-1 GEVIKTVPEAQIVETLIEEALRLADEMGEAPGDAQGSEGA-TGSPIVTVS
MSMEG_2580|M.smegmatis_MC2_155 GEVIKTVPEAQIVETLIEEAMRLAEEIGTAR----GSDDA-SGSPVVTVS
MAB_3169c|M.abscessus_ATCC_199 GEVIKTVPEALIVETLIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS
TH_1399|M.thermoresistible__bu GEVIKTVPESQIVETLIEEAMRIAAEMGSGDGE---SGPESSGSPIVTVS
MMAR_0275|M.marinum_M GEVIKTVPESQIVETLIEQALRIAEERASETEC-----TDLVGTPVVSEV
*********: ******::*:*:. : * *:*: