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GQGTRTLLDMTVWIMRRPEDDIAAELEASSGPLAGVRLAVKDNVDVGGVPTTAACPEYAYVPDQDAPAVA ALRAAGAVVVGKTNLDQFATGLVGTRSPYGAVSDSRRPEYISGGSSSGSAVAVATGQADIAIGTDTAGSG RVPAGLQGIVGIKPTVGVISTEGVVPACESYDCVTIFAPSLTTANAAMAAMGAAAGPRLWPANTRLAAPP QPTIAVPRELPALDEVWCNAFRGAVDDLRAAGATIIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAH PEASLDPTVAQIISGARDIPAHRLVHDRAEVQRLREQAMTSLAGADALLVPTAPLHPTIEQVNANPIGVN ATMGTYTNFCNLFDLCAVSVPAGTAGDAQFGVTVIARAFDDAVAFDIAALATGAPAGQDVWPAAITLSHE LAVFGAHLKGGPLEFQLTDLGARWVGPIRTATKYRMSALRTTPPKPGLTRSEENGVSIGGEVWRLSPAAL GTFLAQLPEPMLLGKVECDDGLWRTGFGCDGAAAQAGVDISEHGSWPAAIAAGAV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. abscessus ATCC 19977 | MAB_1558c | - | - | 100% (546) | allophanate hydrolase |
| M. abscessus ATCC 19977 | MAB_3341c | gatA | 3e-26 | 45.45% (154) | aspartyl/glutamyl-tRNA amidotransferase subunit A |
| M. abscessus ATCC 19977 | MAB_1674c | - | 5e-25 | 29.74% (390) | amidase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2384 | amiA2 | 7e-25 | 40.00% (180) | amidase |
| M. gilvum PYR-GCK | Mflv_2712 | - | 3e-28 | 30.50% (400) | amidase |
| M. tuberculosis H37Rv | Rv2363 | amiA2 | 7e-25 | 40.00% (180) | amidase |
| M. leprae Br4923 | MLBr_01702 | gatA | 2e-26 | 29.47% (414) | aspartyl/glutamyl-tRNA amidotransferase subunit A |
| M. marinum M | MMAR_3673 | amiA2 | 4e-24 | 39.44% (180) | amidase AmiA2 |
| M. avium 104 | MAV_3859 | gatA | 3e-26 | 28.90% (436) | aspartyl/glutamyl-tRNA amidotransferase subunit A |
| M. smegmatis MC2 155 | MSMEG_2189 | atzF | 0.0 | 64.23% (534) | allophanate hydrolase |
| M. thermoresistible (build 8) | TH_0712 | - | 1e-126 | 58.44% (409) | CONSERVED HYPOTHETICAL PROTEIN |
| M. ulcerans Agy99 | MUL_3616 | amiA2 | 2e-24 | 39.44% (180) | amidase |
| M. vanbaalenii PYR-1 | Mvan_4052 | - | 0.0 | 65.06% (541) | amidase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_3673|M.marinum_M MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
MUL_3616|M.ulcerans_Agy99 MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
Mb2384|M.bovis_AF2122/97 MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDV
Rv2363|M.tuberculosis_H37Rv MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDV
Mflv_2712|M.gilvum_PYR-GCK MPDSS--ARSTSRNRFPDRFPTVANQLFQLASGTATSVDLVRRSLVAIEA
MLBr_01702|M.leprae_Br4923 ----------MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEA
MAV_3859|M.avium_104 --------------MNEIIRSDAATLAARIAAKELSSVEATQACLDQIAA
MAB_1558c|M.abscessus_ATCC_199 -------------------------------------------MGQGTRT
Mvan_4052|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_2189|M.smegmatis_MC2_155 ------------------------------------MTGKIDEIYRRIEQ
TH_0712|M.thermoresistible__bu -------------------------------MSAPSAVERVHAAYAAIER
MMAR_3673|M.marinum_M SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDDVD
MUL_3616|M.ulcerans_Agy99 SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDDVD
Mb2384|M.bovis_AF2122/97 SQPTLNAFRVVLTESALADAAAADKRRAAG---DTAPLLGIPIAVKDDVD
Rv2363|M.tuberculosis_H37Rv SQPTLNAFRVVLTESALADAAAADKRRAAG---DTAPLLGIPIAVKDDVD
Mflv_2712|M.gilvum_PYR-GCK SQPTLNAFRVVLAERALADAAEADRLRAAGHDRARRPLLGIPIAVKDDVD
MLBr_01702|M.leprae_Br4923 TDDTYRAFLHVAAEKALSAAAAVDKAVAAG-GQLSSTLAGVPLALKDVFT
MAV_3859|M.avium_104 TDERYHAFLHVAADEALGAAAVVDEAVAAG-ERLPSPLAGVPLALKDVFT
MAB_1558c|M.abscessus_ATCC_199 LLDMTVWIMRRP-----EDDIAAELEAS------SGPLAGVRLAVKDNVD
Mvan_4052|M.vanbaalenii_PYR-1 ---MAVWISRRR-----GPDVAAELSESRS---AAGHLAGVRLAVNDNVD
MSMEG_2189|M.smegmatis_MC2_155 SGRSEVFIHLRP-----RDQVEADHASAT-----SGPLAGLVLAVKDNVD
TH_0712|M.thermoresistible__bu VDRPEIWITLRPLADALADAVRVDAAARTG---AELPLAGLVAAVKNNID
: .: * *: *::: .
MMAR_3673|M.marinum_M VAGVPTAFG-TQGYVRPAAHDSEVVRRLKAAGAVIIGKTNTCELGQWPFT
MUL_3616|M.ulcerans_Agy99 VAGVPTAFG-TQGYVQPAAHDSEVVRRLKAAGAVIIGKTNTCELGQWPFT
Mb2384|M.bovis_AF2122/97 VAGVPTAFG-TQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFT
Rv2363|M.tuberculosis_H37Rv VAGVPTAFG-TQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFT
Mflv_2712|M.gilvum_PYR-GCK VAGVPTRFG-ASGEVPEAVADAEVVRRLRAAGAVIVGKTNTCELGQWPFT
MLBr_01702|M.leprae_Br4923 TVDMPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTNMDEFAMGSST
MAV_3859|M.avium_104 TVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPILGKTNMDEFAMGSST
MAB_1558c|M.abscessus_ATCC_199 VGGVPTTAACPEYAYVPD-QDAPAVAALRAAGAVVVGKTNLDQFATGLVG
Mvan_4052|M.vanbaalenii_PYR-1 VAGLPTTAGCPQFAYLPD-SDAAAVAALRGAGAVVVGKTNLDQFAAGLVG
MSMEG_2189|M.smegmatis_MC2_155 VAGLPTTAACPGYSFVPD-TDAPVVAVLKAAGAVVIGKTNLDQFATGLVG
TH_0712|M.thermoresistible__bu VAGIPTTAGCPTFATCAATADAPAVARLRTAGAVVIGATNLDQFATGLVG
. .:** . *. .. *: ** ::* ** ::.
MMAR_3673|M.marinum_M SGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRVPA
MUL_3616|M.ulcerans_Agy99 SGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRVPA
Mb2384|M.bovis_AF2122/97 SGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPA
Rv2363|M.tuberculosis_H37Rv SGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPA
Mflv_2712|M.gilvum_PYR-GCK SGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGSDGAGSVRIPA
MLBr_01702|M.leprae_Br4923 ENSAYGPTRNPWNVDRVPGGSGGGSAAALAAFQAPLAIGSDTGGSIRQPA
MAV_3859|M.avium_104 ENSAYGPTRNPWNVERVPGGSGGGSAAALAAFQAPLAIGSDTGGSIRQPA
MAB_1558c|M.abscessus_ATCC_199 TRSPYGAVSDSRRPEYISGGSSSGSAVAVATGQADIAIGTDTAGSGRVPA
Mvan_4052|M.vanbaalenii_PYR-1 TRSPYGAVPDSRRPEFISGGPSSGSAVAVATGEADIAIAPDATG--CIPA
MSMEG_2189|M.smegmatis_MC2_155 TRSPYGPVRNAQRPEYISGGSSSGSAVAVALGFADIAIGTDTAGSGRVPA
TH_0712|M.thermoresistible__bu TRSPYGAVRDARRPDRISGGSSSGSAVAVALGLVDVALGTDTAGSGRVPA
. :* . :. .**...***.*:* . *:..* * **
MMAR_3673|M.marinum_M AWTHLVGIKPQRGRISTWPWPETFNGITVNGVLARTVADAALVLDAASGN
MUL_3616|M.ulcerans_Agy99 AWTHLVGIKPQRGRISTWPWPETFNGITVNGVLARTVADAALVLDAASGN
Mb2384|M.bovis_AF2122/97 AWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVLDAASGN
Rv2363|M.tuberculosis_H37Rv AWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVLDAASGN
Mflv_2712|M.gilvum_PYR-GCK AWTHLVGIKPQRGRISTSPLPEAFNGITVNGVLARTVSDAAMVLDAAAGN
MLBr_01702|M.leprae_Br4923 ALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGH
MAV_3859|M.avium_104 ALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGH
MAB_1558c|M.abscessus_ATCC_199 GLQGIVGIKPTVGVISTEGVVPACESYDCVTIFAPSLTTANAAMAAMGAA
Mvan_4052|M.vanbaalenii_PYR-1 ALQGIVAIKPTVGVISTDGAVPASESYDCITILAATLNLADTAMAVLAAG
MSMEG_2189|M.smegmatis_MC2_155 GLQGIVGIKPTLGVVPNTGVVPACASYDCVTIFAADLPTAQAAMGVMATA
TH_0712|M.thermoresistible__bu ALQGIVGIKPTIGVVPTDGVVPACRSYDCVTVFARDLDTADAAMGVLAGG
. *.:** * :. . * : : . .
MMAR_3673|M.marinum_M VE-----GDLHKPAPLTASDYVGIAPGPLKIAMSTRFPYTGFR--AKLHP
MUL_3616|M.ulcerans_Agy99 AE-----GDLHKPAPLTASDYVGIAPGPLKIAMSTRFPYTGFR--AKLHP
Mb2384|M.bovis_AF2122/97 VE-----GDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFR--AKLHP
Rv2363|M.tuberculosis_H37Rv VE-----GDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFR--AKLHP
Mflv_2712|M.gilvum_PYR-GCK AA-----GDLHTPAPVTVSDFVSRAPGPLRVAVSTRFPFTGFR--ATLHP
MLBr_01702|M.leprae_Br4923 DA-----RDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQS
MAV_3859|M.avium_104 DI-----RDSTSVDAPVPDVVGAARAGAAGDLKGVRVGVVKQLRGEGYQP
MAB_1558c|M.abscessus_ATCC_199 AGPRLWPANTRLAAPPQPTIAVPRELPALDEVWCNAFRGAVDDLRAAGAT
Mvan_4052|M.vanbaalenii_PYR-1 APARPWPTDVKLAAPPVPVIAIPDELPYLDDQWRVAFDAAVDTLVARGAR
MSMEG_2189|M.smegmatis_MC2_155 EPHRAWPADLMLAAPPAPRVAVPAQVPELDVAWQAAFTEAVQRAQAAGFE
TH_0712|M.thermoresistible__bu G--RPFPPDAPLTVPPTTRVAVPGRLPGLSPAWRQAFESACERLAGNGIE
: . . .
MMAR_3673|M.marinum_M EILAATRRVGEQLQLLGHTVERGNP---DYGLRLSWDFLARSTAG-----
MUL_3616|M.ulcerans_Agy99 EILASTRRVGEQLQLLGHTVERGNP---DYGLRLSWDFLARSTAG-----
Mb2384|M.bovis_AF2122/97 EILAATQRVGDQLELLGHTVVKGNP---DYGLRLSWNFLARSTAG-----
Rv2363|M.tuberculosis_H37Rv EILAATQRVGDQLELLGHTVVKGNP---DYGLRLSWNFLARSTAG-----
Mflv_2712|M.gilvum_PYR-GCK EIRSALDGVADQLSQLGHTVLNADP---DYTLGMSWNFLARSTSG-----
MLBr_01702|M.leprae_Br4923 GVLASFQAAVEQLIALGATVSEVDCPHFDYALAAYYLILPSEVSSNLARF
MAV_3859|M.avium_104 GVLASFEAAVEQLTALGAEVSEVDCPHFEYALAAYYLILPSEVSSNLARF
MAB_1558c|M.abscessus_ATCC_199 IIEIDLTPFLAAAKLLYEGALVSERYAAVGEFIDAH---PEASLDPTVAQ
Mvan_4052|M.vanbaalenii_PYR-1 VVSVEIEPFLTAATLLHDGALVSERYAAVGEFVDGQ---PAAALDPTVRD
MSMEG_2189|M.smegmatis_MC2_155 TVPIDMTPFFAAADLLYNGALVAERWDAVGEFIESAG--PGAGLDPTVSA
TH_0712|M.thermoresistible__bu LVEVDLTPFLQAATLLYDGGLVAERHAAVGTFVDEHLAAGDPALDPTVAQ
: * : : .
MMAR_3673|M.marinum_M -LWEWAECLGDGVTLDPRTVTNMRAGHALSQAILR---------------
MUL_3616|M.ulcerans_Agy99 -LWEWAECLGNGVTLDPRTVTNMRAGHALSQAILR---------------
Mb2384|M.bovis_AF2122/97 -LWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILR---------------
Rv2363|M.tuberculosis_H37Rv -LWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILR---------------
Mflv_2712|M.gilvum_PYR-GCK -IPEWMDRLGHRVTWDSRTRSNARMGRLLSQHTLR---------------
MLBr_01702|M.leprae_Br4923 DAMRYGLRVGDDGSRSAEEVIALTRAAGFGPEVKRRIMIGAYALSAGYYD
MAV_3859|M.avium_104 DAMRYGLRIGDDGSHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYD
MAB_1558c|M.abscessus_ATCC_199 IISGARDIPAHRLVHDRAEVQRLREQAMTSLAGADALLVPTAPLHPTIEQ
Mvan_4052|M.vanbaalenii_PYR-1 IITAARDVPAHRLLRDRAAVGRLRDRAMAALAGTDALLVPTAPVHPTIAD
MSMEG_2189|M.smegmatis_MC2_155 IISAAGRHSAADLLRDRRRVEHLRDEAFAALAGCDALLVPTAPEHPRIAD
TH_0712|M.thermoresistible__bu IISAAGAVPATTLLRDRARLAELTASALARLGDCAALLIPTTTEHPSIAE
. .
MMAR_3673|M.marinum_M -----NARHHEAAAQRRVGSIFDIVDVVLAPTTAQP------PPLARAFD
MUL_3616|M.ulcerans_Agy99 -----NARHHEAAAQRRVGSIFDIVDVVLAPTTAQP------PPLARAFD
Mb2384|M.bovis_AF2122/97 -----SARRHEAADQRRVGSIFDIVDVVLAPTTAQP------PPMARAFD
Rv2363|M.tuberculosis_H37Rv -----SARRHEAADQRRVGSIFDIVDVVLAPTTAQP------PPMARAFD
Mflv_2712|M.gilvum_PYR-GCK -----KARAREAAAQQRIGWIFNLADVVVAPTTAQP------PPEAHAFD
MLBr_01702|M.leprae_Br4923 SYYS-QAQKVRTLIARDLDKAYRSVDVLVSPATP-----------TTAFS
MAV_3859|M.avium_104 AYYN-QAQKVRTLIARDLDAAYESVDVVVSPATP-----------TTAFG
MAB_1558c|M.abscessus_ATCC_199 VNANPIGVNATMGTYTNFCNLFDLCAVSVPAGTAGD-AQFGVTVIARAFD
Mvan_4052|M.vanbaalenii_PYR-1 ATADPVEINSRLGTYTNFCNLFDMCAIAVPAGTAGG-AQFGVTVLARAFE
MSMEG_2189|M.smegmatis_MC2_155 VAADPIGVNARMGRFTNFCNLFDMCAIAIPAGTTSDGAQFGITLLAPAFG
TH_0712|M.thermoresistible__bu VAADPVGVNSRLGTYTNFCNLMDLCAVAVPAGTTSDGAQFGVTVLGRAGA
. : :.. *. *
MMAR_3673|M.marinum_M DMGGLATDRAIIAACPLTWPWNLLGWPSINVPAGFT--------------
MUL_3616|M.ulcerans_Agy99 DMGGLATDRAIIAACPLTWPWNLLGWPSINVPAGFT--------------
Mb2384|M.bovis_AF2122/97 RLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFT--------------
Rv2363|M.tuberculosis_H37Rv RLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFT--------------
Mflv_2712|M.gilvum_PYR-GCK GLGGLATDRMMIRACPVTWPWNLLGWPSINVPAGFT--------------
MLBr_01702|M.leprae_Br4923 LGEKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLS--------------
MAV_3859|M.avium_104 LGEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLS--------------
MAB_1558c|M.abscessus_ATCC_199 DAVAFDIAALATGAPAGQDVWPAAITLSHELAVFG---AHLK-----GGP
Mvan_4052|M.vanbaalenii_PYR-1 DAVALDVAALLVDEAPSASPWPLAAAEVVELVVFG---AHLR-----GGP
MSMEG_2189|M.smegmatis_MC2_155 DAVVADLAARFLAVPAAAS-WAEAATEVTEIAVFG---AHLR-----GQP
TH_0712|M.thermoresistible__bu DALALDLARLVSAPVDGAAPPGVPAPPTPAVPWPXXXAADLTDDDHLGRP
:
MMAR_3673|M.marinum_M -VDG-LPIGVQLMGPAESEGLLISLAAELEAVSGWATKQPKVWWNTGTST
MUL_3616|M.ulcerans_Agy99 -VDG-LPIGVQLMGPAESEGLLISLAAELEAVSGWATKQPKVWWNTGTST
Mb2384|M.bovis_AF2122/97 -SDG-LPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQVWWTS----
Rv2363|M.tuberculosis_H37Rv -SDG-LPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQVWWTS----
Mflv_2712|M.gilvum_PYR-GCK -SDG-LPIGVQLMGPADSEPLLISLAAALEAINGWATLQPENWWAPQPPT
MLBr_01702|M.leprae_Br4923 -SDDDLPVGLQIMAPALADDRLYRVGAAYEAARGPLPSAI----------
MAV_3859|M.avium_104 -PDDDLPVGLQIMAPALADDRLYRVGAAYEAARGPLRSAI----------
MAB_1558c|M.abscessus_ATCC_199 LEFQLTDLGARWVGPIRT-ATKYRMSALRTTPPKPGLTRSEE--NGVSIG
Mvan_4052|M.vanbaalenii_PYR-1 LVHQLTDRGARFAGEITT-APRYRMTALPTTPPKPGVTRVPDGVEGAALS
MSMEG_2189|M.smegmatis_MC2_155 LEHELTGRGARWGGPVVT-APHYRLFALDTEPPKPGLVRVDS--DGSAIE
TH_0712|M.thermoresistible__bu RGDTETGQRLELLAHLVTGGTRAPATVLAAVVHGELLALAPFGTADGVVA
. . : .
MMAR_3673|M.marinum_M PPIHSVRPPRR---------------------------------------
MUL_3616|M.ulcerans_Agy99 PPIHSVRPPRR---------------------------------------
Mb2384|M.bovis_AF2122/97 --------------------------------------------------
Rv2363|M.tuberculosis_H37Rv --------------------------------------------------
Mflv_2712|M.gilvum_PYR-GCK EPDLTAPDSVTGQVA-----------------------------------
MLBr_01702|M.leprae_Br4923 --------------------------------------------------
MAV_3859|M.avium_104 --------------------------------------------------
MAB_1558c|M.abscessus_ATCC_199 GEVWRLSPAALGTFLAQLPEPMLLGKVECDD-GLWRTGFGCDG-AAAQAG
Mvan_4052|M.vanbaalenii_PYR-1 GHRWLLSPAALGSFLAALPAPMQLGAVEFAD-GSWRTAFGCD---ASVQG
MSMEG_2189|M.smegmatis_MC2_155 GERWVLSPAALGEFLAELPAPMLLGKVELSD-GRWVTGFGCDH-AAPAHG
TH_0712|M.thermoresistible__bu RAASRLVTMASGLDPHGLGVPEVFWMTQQRDYRAAAQGFASGTRDGLTTW
MMAR_3673|M.marinum_M --------------------------
MUL_3616|M.ulcerans_Agy99 --------------------------
Mb2384|M.bovis_AF2122/97 --------------------------
Rv2363|M.tuberculosis_H37Rv --------------------------
Mflv_2712|M.gilvum_PYR-GCK --------------------------
MLBr_01702|M.leprae_Br4923 --------------------------
MAV_3859|M.avium_104 --------------------------
MAB_1558c|M.abscessus_ATCC_199 VDISEHGSWPAAIAAGAV--------
Mvan_4052|M.vanbaalenii_PYR-1 VDISGYGSWTSALASGAVRQT-----
MSMEG_2189|M.smegmatis_MC2_155 RDITEFGGWLRAQSALSAR-------
TH_0712|M.thermoresistible__bu LMLTARALHAGAREAISIAEAQAEAN