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VMSASWLRHRVSERGLIATAEQLWADSFRLALVAAHDDGDSLRVVYLFLAGYPDRRVELEYVVPADNPEI RSLAYLSFPAGRFEREMADLYGIRPVGHPKPRRLVRHAHWPDWHPMRTDAGPAPEFTDTGAFPFLAVEGP GVYEIPVGPVHAGLIEPGHFRFSVAGETIVRLKARLWFVHRGIEKLFHGRPATAAVDLAERISGDTSAAH ALAHSLAIEDALGIELPHEVHRLRALIVELERLYNHAADLGALANDVGYSLANAHAQRIRENLLRRNAAV TGHRLLRGAIRAGGVALRALPDTDELAALAVDLAEVATLTLANSVVYDRFAGTAVLHPDDASALGCLGYV ARASGLRSDARVEHPTIVLPITEIGAPDGDVLARYTVRRDEFAASAALAQHIVESHTGPIEYAATLHPVG APSSGIGIVEGWRGTIVHRVEIDVDGRITRAKVVDPSWFNWPALPVAMADTIVPDFPLANKSFNQSYAGN DL
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. tuberculosis H37Rv | Rv0087 | hycE | - | 100% (492) | POSSIBLE FORMATE HYDROGENASE HYCE (FHL) |
| M. tuberculosis H37Rv | Rv3148 | nuoD | 1e-17 | 27.41% (270) | NADH dehydrogenase subunit D |
| M. tuberculosis H37Rv | Rv3147 | nuoC | 4e-05 | 35.29% (85) | NADH dehydrogenase subunit C |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0090 | hycE | 0.0 | 99.80% (492) | formate hydrogenase HycQ |
| M. gilvum PYR-GCK | Mflv_4484 | - | 5e-17 | 27.78% (270) | NADH dehydrogenase subunit D |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_2137 | - | 2e-19 | 28.78% (278) | NADH dehydrogenase subunit D |
| M. marinum M | MMAR_1659 | hycE | 0.0 | 70.48% (498) | formate hydrogenase HycE |
| M. avium 104 | MAV_5109 | - | 0.0 | 76.83% (492) | hydrogenase-4, G subunit, putative |
| M. smegmatis MC2 155 | MSMEG_2060 | nuoD | 4e-15 | 25.99% (277) | NADH dehydrogenase subunit D |
| M. thermoresistible (build 8) | TH_0634 | nuoD | 6e-15 | 26.75% (228) | PROBABLE NADH DEHYDROGENASE I (CHAIN D) NUOD |
| M. ulcerans Agy99 | MUL_2462 | nuoD | 7e-18 | 27.41% (270) | NADH dehydrogenase subunit D |
| M. vanbaalenii PYR-1 | Mvan_1882 | - | 1e-15 | 27.41% (270) | NADH dehydrogenase subunit D |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_2060|M.smegmatis_MC2_155 --------------------------------------------------
TH_0634|M.thermoresistible__bu --------------------------------------------------
Mflv_4484|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_1882|M.vanbaalenii_PYR-1 --------------------------------------------------
MUL_2462|M.ulcerans_Agy99 --------------------------------------------------
MAB_2137|M.abscessus_ATCC_1997 --------------------------------------------------
Rv0087|M.tuberculosis_H37Rv MMSASWLRHRVSERGLIATAEQLWADSFRLALVAAHDDGDSLRVVYLFLA
Mb0090|M.bovis_AF2122/97 MMSASWLRHRVSERGLIATAEQLWADSFRLALVAAHDDGDSLRVVYLFLA
MAV_5109|M.avium_104 -MTPEWSRQPVSSDELRDRAEELTTNGFRLALVAAHDDADRLRVVYLFLA
MMAR_1659|M.marinum_M -MAHKTVSRTVSDAELAESVAELLAEGYRLALVAAHDDGDALRVVYLLVA
MSMEG_2060|M.smegmatis_MC2_155 ------------------------------------------MSTSTVPP
TH_0634|M.thermoresistible__bu --------------------------------------------MSTRP-
Mflv_4484|M.gilvum_PYR-GCK ------------------------------------------MTTSAQRP
Mvan_1882|M.vanbaalenii_PYR-1 ------------------------------------------MTTSQQPP
MUL_2462|M.ulcerans_Agy99 ------------------------------------------MTDT----
MAB_2137|M.abscessus_ATCC_1997 ------------------------------------------MTAQP---
Rv0087|M.tuberculosis_H37Rv GYPDRRVELEYVVPADNPEIRSLAYLSFPAGRFEREMADLYGIRPVGHPK
Mb0090|M.bovis_AF2122/97 GYPDRRVELEYVVPADNPEIRSLAYLSFPAGRFEREMADLYGIRPVGHPK
MAV_5109|M.avium_104 GRPDRRVELECVLPVDDPSLPSLAYLSFPAGRFEREMLDLYGISPIGHPR
MMAR_1659|M.marinum_M GQPDRRVELTLRTGAANPSVPSLAELSIPAGRFEREMMDLYGISPVGHPQ
MSMEG_2060|M.smegmatis_MC2_155 DGGEKVVVVGGNDWHEVVAAARAG-----------AAAQAGERIVVNMGP
TH_0634|M.thermoresistible__bu ---ETVITLGGQDWDQLVAEARSN-----------AADQTGERIVVNMGP
Mflv_4484|M.gilvum_PYR-GCK ---ERVIVVGGQDWDQVVTAARQMSNSSSR--AGDAAEHAGERIVVNMGP
Mvan_1882|M.vanbaalenii_PYR-1 ---ERVVVVGGQDWDQVVAAARQN-----------AAEHAGERIVVNMGP
MUL_2462|M.ulcerans_Agy99 ---ETVLVAGGQDWNEIVEAARGA--------------DPGERIVVNMGP
MAB_2137|M.abscessus_ATCC_1997 ---EIHLMAGGQDWDEIVTAAGQA---------------TDERIVVNMGP
Rv0087|M.tuberculosis_H37Rv PRRLVRHAHWPD-WHPMRTDAGPAPEFTDTGAFPFLAVEGPGVYEIPVGP
Mb0090|M.bovis_AF2122/97 PRRLVRHAHWPD-WHPMRTDAGPAPEFTDTGAFPFLAVEGPGVYEIPVGP
MAV_5109|M.avium_104 PRRLVRHAHWPEAWYPMRADAGPAPEFTATGHFPFVTVDGTGVYEIPVGP
MMAR_1659|M.marinum_M PRRLVRHAHWPQDWHPMRRDAGDPPPFDAVGRFPFVTVEGPGVYEIPVGP
: * : * : :**
MSMEG_2060|M.smegmatis_MC2_155 QHPSTHGVLRLILEIEGEIITEARCGIGYLHTGIEKNLEYR---NWTQGV
TH_0634|M.thermoresistible__bu QHPSTHGVLRLVLEIEGETVTEARCGVGYLHTGIEKNLEYR---TWMQGV
Mflv_4484|M.gilvum_PYR-GCK QHPSTHGVLRLILEIEGETIVEARCGIGYLHTGIEKNLEFR---TWTQGV
Mvan_1882|M.vanbaalenii_PYR-1 QHPSTHGVLRLILEIEGEIIVEARCGIGYLHTGIEKNLEFR---NWTQGV
MUL_2462|M.ulcerans_Agy99 QHPSTHGVLRLILEIEGETVTEARCGIGYLHTGIEKNLEYR---YWTQGV
MAB_2137|M.abscessus_ATCC_1997 QHPSTHGVLRLILEIEGETVTDVRCGIGYLHTGIEKNLEYR---TWTQGV
Rv0087|M.tuberculosis_H37Rv VHAGLIEPGHFRFSVAGETIVRLKARLWFVHRGIEKLFHGR---PATAAV
Mb0090|M.bovis_AF2122/97 VHAGLIEPGHFRFSVAGETIVRLKARLWFVHRGIEKLFHGR---PATAAV
MAV_5109|M.avium_104 VHAGLIEPGHFRFSVVGETVLRLKARLWFVHRGLEQLFHGR---IASGAV
MMAR_1659|M.marinum_M VHAGLIEPGHFRFSVIGESILRLKARLWFVHRGVEKLFEGRNALGAREAV
*.. :: :.: ** : :. : ::* *:*: :. * .*
MSMEG_2060|M.smegmatis_MC2_155 TFVTRMDYLSPFFNETAYCLGVEKLLGITDDIPERASVIRVMLMELNRIS
TH_0634|M.thermoresistible__bu TFVTRMDYLSPFFNETVYCLGVEKLLGITEAIPERANVIRVMLMELNRIS
Mflv_4484|M.gilvum_PYR-GCK TFVTRMDYLSPFFNETAYCLGVEKLLGVTDDIPERASVIRVMMMELNRIS
Mvan_1882|M.vanbaalenii_PYR-1 TFVTRMDYLSPFFNETAYCLGVEKLLGITDAIPERASVIRVMMMELNRIS
MUL_2462|M.ulcerans_Agy99 TFVTRMDYLSPFFNETAYCLGVEKLLGITDEIPERVNVIRVMMMELNRIS
MAB_2137|M.abscessus_ATCC_1997 TFVTRMDYLSPFFNETAYCLGVEQLLGIADEIPERANVIRVLMMELNRIS
Rv0087|M.tuberculosis_H37Rv DLAERISGDTSAAHALAHSLAIEDALGI--ELPHEVHRLRALIVELERLY
Mb0090|M.bovis_AF2122/97 DLAERISGDTSAAHALAHSLAIEDALGI--ELPHEIHRLRALIVELERLY
MAV_5109|M.avium_104 DLAERVSGDTSAAHALAHSLAVEDALGI--VVPNNVHRLRALLIELERLY
MMAR_1659|M.marinum_M ELAERISGDSSAAHSLAHCLAVEDALGL--DVPEPVHRLRALLVELERLY
:. *:. :. : .:.*.:*. **: :*. :*.:::**:*:
MSMEG_2060|M.smegmatis_MC2_155 SHLVALATGGMELGAMSAMFYGFREREEILRVFESITGLRMNHAYIRPGG
TH_0634|M.thermoresistible__bu SHLVALATGGMELGAMTAMFLGFREREEILKVFELITGLRMNHAYIRPGG
Mflv_4484|M.gilvum_PYR-GCK SHLVALATGGMELGAMTAMFLGFRERELILSVFETITGLRMNSAYIRPGG
Mvan_1882|M.vanbaalenii_PYR-1 SHLVALATGGMELGAMTAMFLGFRERELILSVFETITGLRMNNAYIRPGG
MUL_2462|M.ulcerans_Agy99 SHLVALAAGGMELGAMTPMFVGFRAREIILSLFESITGLRMNSAYIRPGG
MAB_2137|M.abscessus_ATCC_1997 SHLVALATGGMELGAMTAMFLGFREREMILKVFEAITGLRMNHAYVRPGG
Rv0087|M.tuberculosis_H37Rv NHAADLGALANDVGYSLANAHAQRIRENLLRRNAAVTGHRLLRGAIRAGG
Mb0090|M.bovis_AF2122/97 NHAADLGALANDVGYSLANAHAQRIRENLLRRNAAVTGHRLLRGAIRAGG
MAV_5109|M.avium_104 NHAADLGALANDVGFALANAHALRIREELLRINAAVTGHRLLRGAIRPGG
MMAR_1659|M.marinum_M NHVTDLGALANDVGFALANAHAQGIRERVLRINRRVTGHRLLRAAMAPGG
.* . *.: . ::* . . ** :* :** *: . : .**
MSMEG_2060|M.smegmatis_MC2_155 LAADLPDDAITQVRRLVEILPKRLKDLEDLLNENYIWKARTVGVGYLDLT
TH_0634|M.thermoresistible__bu LAADLPPDAVPRVRELLELLPGRLRDLEDLLSQNHIWRARNEGVGYLDLT
Mflv_4484|M.gilvum_PYR-GCK VAADLPEEGLPQIRELLTLLPTRLRDMENLLNENYIWKARTLGVGYLDLT
Mvan_1882|M.vanbaalenii_PYR-1 VAADLPDEALPQVRDLLTLLPKRLRDMEDLLNENYIWKARTQGIGYLDLT
MUL_2462|M.ulcerans_Agy99 VAQDLPPDRPTEIAKAIADLRQPLREMGDLLNENAIWKARTQDVGYLDLA
MAB_2137|M.abscessus_ATCC_1997 LAQDLPDGAEQEVRDLLEILPGRLRDMENLLNENYIWKARTQGVGYLDLT
Rv0087|M.tuberculosis_H37Rv VALRALPDT-----DELAALAVDLAEVATLTLANSVVYDRFAGTAVLHPD
Mb0090|M.bovis_AF2122/97 VALRALPDT-----DELAALAVDLAEVATLTLANSVVYDRFAGTAVLHPD
MAV_5109|M.avium_104 VTLHALPDA-----RQLRSIAADLAEVAELTLGNSVIYDRFAGTAILPAD
MMAR_1659|M.marinum_M VVLQELPDP-----AELHAVAAEVAEISELTLRNSVVYDRFAETAVLHSE
:. : : : :: * * : * . *
MSMEG_2060|M.smegmatis_MC2_155 GCMALGITGPILRSTGLPHDLRKAQPYCGYENYEFDVITDDRCDSYGRYI
TH_0634|M.thermoresistible__bu GCMALGVTGPVLRSAGLPHDLRKSQPYCGYENYEFDVITDDRCDAYGRFM
Mflv_4484|M.gilvum_PYR-GCK GCMALGITGPVLRSTGLPHDLRKSQPYCGYQTYDFDVITDDRCDSYGRYL
Mvan_1882|M.vanbaalenii_PYR-1 GCMALGITGPVLRSTGLPHDLRKAQPYCGYETYDFDVVTDDQCDSYGRYL
MUL_2462|M.ulcerans_Agy99 GCMALGITGPILRSTGLPHDLRKSEPYCGYENYEFDVITADGCDAYGRYM
MAB_2137|M.abscessus_ATCC_1997 GCMALGITGPVLRATGLAHDLRRAQPYCGYENYDFDVVTHQDCDSYGRYL
Rv0087|M.tuberculosis_H37Rv DASALGCLGYVARASGLRSDARVEHPTIVLP---ITEIGAPDGDVLARYT
Mb0090|M.bovis_AF2122/97 DASALGCLGYVARASGLRSDARVEHPTIVLP---ITEIGAPDGDVLARYT
MAV_5109|M.avium_104 DACAMGCLGYVARASGIRTDARVEHPSVALP---VTEITQSAGDVLARYT
MMAR_1659|M.marinum_M DARALGCLGYVARASGIATDARLDHPTAELP---VTAVAAGGGDVLARYT
.. *:* * : *::*: * * .* . : * .*:
MSMEG_2060|M.smegmatis_MC2_155 IRVKEMHESVKIVEQCLARLKP---GPVMISDKKLAWPADLKLGPDGLGN
TH_0634|M.thermoresistible__bu IRVKEMHESLKIVEQCLDRLRP---GPVMIEDRKLAWPADLKLGPDGLGN
Mflv_4484|M.gilvum_PYR-GCK IRVKEMRESLRIVEQCVERLERSTGEPVMITDRKLAWPADLKVGPDGLGN
Mvan_1882|M.vanbaalenii_PYR-1 IRVKEMHQSIRIVEQCVQRLERSVGAPVMITDKKLAWPADLKVGPDGLGN
MUL_2462|M.ulcerans_Agy99 IRVKEMWESIKIVEQCLDRLRP---GPTMIEDRKLAWPADLKVGPDGMGN
MAB_2137|M.abscessus_ATCC_1997 IRVKEMHESIKIAQQCVDRLRP---GPVMVDDKKIAWPADLASGPDGLGN
Rv0087|M.tuberculosis_H37Rv VRRDEFAASAALAQHIVESHTG---------------PIEYAATLHPVGA
Mb0090|M.bovis_AF2122/97 VRRDEFAASAALAQHIVESHTG---------------PIEYAATLHPVGA
MAV_5109|M.avium_104 VRRDEFAASTTLATYLAESHTG---------------TLDYHTPTPVVGG
MMAR_1659|M.marinum_M IRRDEFAASVALACHLVENHHG---------------RTRFAPPPAANGA
:* .*: * :. *
MSMEG_2060|M.smegmatis_MC2_155 SPEHIAKIMGRSMEGLIHHFKLVTEGIRVPPGQVYVAVESPRGELGVHMV
TH_0634|M.thermoresistible__bu SPEHVARIMGRSMEGLIHHFKLVTEGIRVPPGQVYVAVESPRGELGVHMV
Mflv_4484|M.gilvum_PYR-GCK SPEHIAKIMGHSMEGLIHHFKLVTEGIRVPPGQVYVAVESPRGELGVHMV
Mvan_1882|M.vanbaalenii_PYR-1 SPEHIAKIMGHSMEGLIHHFKLVTEGIRVPAGQVYVAVESPRGELGVHMV
MUL_2462|M.ulcerans_Agy99 SPEHIAKIMGSSMEALIHHFKLVTEGIRVPAGQVYVAVESPRGELGVHMV
MAB_2137|M.abscessus_ATCC_1997 SPKHIAKIMGTSMEGLIHHFKLVTEGVRVPAGQVYVAIESPRGELGVHMV
Rv0087|M.tuberculosis_H37Rv P----------------------SSGIGIVEG--------WRGTIVHRVE
Mb0090|M.bovis_AF2122/97 P----------------------SSGIGIVEG--------WRGTIVHRVE
MAV_5109|M.avium_104 P----------------------RSGVGIVEG--------WRGTIVHRVE
MMAR_1659|M.marinum_M RS-------------------AVTSGVGIVEG--------WRGAVLHRVE
.*: : * ** : ::
MSMEG_2060|M.smegmatis_MC2_155 SDGGTRPYRVHYRDPSFTNLQAVAATCEGGMVADAIAAVASIDPVMGGVD
TH_0634|M.thermoresistible__bu SDGGTRPYRVHYRDPSFTNLQAVAAMAEGGMVADLIAALASIDPVMGGVD
Mflv_4484|M.gilvum_PYR-GCK SDGGTRPYRVHYRDPSFTNLQAVAAMCEGGMVADAITAVASIDPVMGGVD
Mvan_1882|M.vanbaalenii_PYR-1 SDGGTRPYRVHYRDPSFTNLQAVAAMCEGGMVADAITAVASIDPVMGGVD
MUL_2462|M.ulcerans_Agy99 SDGGTRPYRVHYRDPSFTNLQAVAAMSEGGMVADLITAVASIDPVMGGVD
MAB_2137|M.abscessus_ATCC_1997 SDGGTRPYRVHFRDPSFTNLQSVAAMCEGAMVADLIAAVASIDPVMGGVD
Rv0087|M.tuberculosis_H37Rv IDVDGRITRAKVVDPSWFNWPALPVAMADTIVPDFPLANKSFNQSYAGND
Mb0090|M.bovis_AF2122/97 IDVDGRITRAKVVDPSWFNWPALPVAMADTIVPDFPLANKSFNQSYAGND
MAV_5109|M.avium_104 IAADCRITRAKIVDPSWFNWPALPVAMADTIVPDFPLTNKSFNLSYAGND
MMAR_1659|M.marinum_M VDGAGRITRCKIVDPSWFNWPALPVAMADTIVPDFPLANKSFNLSYAGND
* * : ***: * ::.. . :*.* : *:: .* *
MSMEG_2060|M.smegmatis_MC2_155 R
TH_0634|M.thermoresistible__bu R
Mflv_4484|M.gilvum_PYR-GCK R
Mvan_1882|M.vanbaalenii_PYR-1 R
MUL_2462|M.ulcerans_Agy99 R
MAB_2137|M.abscessus_ATCC_1997 R
Rv0087|M.tuberculosis_H37Rv L
Mb0090|M.bovis_AF2122/97 L
MAV_5109|M.avium_104 L
MMAR_1659|M.marinum_M L