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TTIAFLGLGNMGGPMAANLVAAGQTVHAFDLVTELKEAAKAKGAKVFDSGAAAVADADVVITSLPNGNIV KAVYDEVVPAAKAGALLIDTSTISVDDARAIHDQATEKGLAQIDAPVSGGIKGATAGTLAFMVGGEDEAF ERAKPVLEPMAGKIIHCGASGAGQAAKLCNNMVLAVQQIAIGEAFVLAEKLGLPAQSLFDVITGATGNCW AVHTNCPVPGPVPTSPANNDFKPGFATALMNKDLGLAMAAVSSTGSSAPLGTHAAEIYAEFNKEHADKDF SAIIEALRG*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_1360 | - | - | 100% (290) | 3-hydroxyisobutyrate dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_4822 | - | 2e-33 | 31.25% (288) | 3-hydroxyisobutyrate dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_5155 | - | 4e-30 | 34.36% (259) | 6-phosphogluconate dehydrogenase, NAD-binding |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0773c | mmsB | 1e-117 | 71.63% (289) | 3-hydroxyisobutyrate dehydrogenase MmsB |
| M. gilvum PYR-GCK | Mflv_5012 | - | 1e-150 | 91.70% (289) | 3-hydroxyisobutyrate dehydrogenase |
| M. tuberculosis H37Rv | Rv0751c | mmsB | 1e-117 | 71.63% (289) | 3-hydroxyisobutyrate dehydrogenase MmsB |
| M. leprae Br4923 | MLBr_02065 | gnd | 2e-11 | 25.93% (216) | 6-phosphogluconate dehydrogenase |
| M. abscessus ATCC 19977 | MAB_1186c | - | 7e-91 | 57.39% (291) | 3-hydroxyisobutyrate dehydrogenase |
| M. marinum M | MMAR_1077 | mmsB | 1e-126 | 77.35% (287) | 3-hydroxyisobutyrate dehydrogenase, MmsB |
| M. avium 104 | MAV_4419 | mmsB | 1e-124 | 76.66% (287) | 3-hydroxyisobutyrate dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_1496 | mmsB | 1e-136 | 81.72% (290) | 3-hydroxyisobutyrate dehydrogenase |
| M. thermoresistible (build 8) | TH_0328 | mmsB | 1e-126 | 81.14% (281) | PROBABLE 3-HYDROXYISOBUTYRATE DEHYDROGENASE MMSB (HIBADH) |
| M. ulcerans Agy99 | MUL_0835 | mmsB | 1e-125 | 76.66% (287) | 3-hydroxyisobutyrate dehydrogenase, MmsB |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0773c|M.bovis_AF2122/97 ------MTT---IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAA
Rv0751c|M.tuberculosis_H37Rv ------MTT---IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAA
MMAR_1077|M.marinum_M ------MTKSCQIAFLGLGHMGAPMSANLIGAGHTVRGFDPVPAAASAAA
MUL_0835|M.ulcerans_Agy99 ------MTKSCQIAFLGLGHMGAPMSANLIGAGHTVRGFDPVPAAASATA
MAV_4419|M.avium_104 ----------MVVAFLGLGNMGAPMAANLVAAQHAVRGFDPVPAALSAAT
Mvan_1360|M.vanbaalenii_PYR-1 ------MTT---IAFLGLGNMGGPMAANLVAAGQTVHAFDLVTELKEAAK
Mflv_5012|M.gilvum_PYR-GCK ------MTT---IAFLGLGNMGGPMAANLVAAGHTVHAFDVVPELRDAAE
MSMEG_1496|M.smegmatis_MC2_155 ------MAT---IAFLGLGHMGGPMAANLAAAGHTVRGFDPMPASREAAE
TH_0328|M.thermoresistible__bu --------------------MGGPMAANLVAAGHTVRGFDPVAESRAAAA
MAB_1186c|M.abscessus_ATCC_199 ------MTN---IAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAAT
MLBr_02065|M.leprae_Br4923 MSAPESKTAIAQIGVTGLAVMGSNIARNFARHGYTVALHNRSIAKTDTLL
** :: *: .* .:
Mb0773c|M.bovis_AF2122/97 AHG--------VAVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLAAAR
Rv0751c|M.tuberculosis_H37Rv AHG--------VAVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLAAAR
MMAR_1077|M.marinum_M AKG--------ATMFDSAAEAVAGADVVITMLPNGAVVKRCYAEVLPAAR
MUL_0835|M.ulcerans_Agy99 AKG--------ATMFDSAAEAVARADVVITMLPNGAVVKRCYAEVLPAAR
MAV_4419|M.avium_104 ASG--------VTGFDTATDAVTGADVVITMLPNGEVVKRCYAEILPAAR
Mvan_1360|M.vanbaalenii_PYR-1 AKG--------AKVFDSGAAAVADADVVITSLPNGNIVKAVYDEVVPAAK
Mflv_5012|M.gilvum_PYR-GCK AKG--------ANVFDSAAAAVADADVVVTSLPNGAVVKAVYDEVVPAAK
MSMEG_1496|M.smegmatis_MC2_155 AKG--------IKVFGTGAEAASEAEVVITSLPNGDIVKSCYAEVLPAAP
TH_0328|M.thermoresistible__bu DKG--------VQTFDSGAEAVAGAEVVITSLPNGDIVKQCYAEVLPAAT
MAB_1186c|M.abscessus_ATCC_199 QAG--------VPLAESGAAAASTADVVITMLPKGAHVLAAYEDLLPAAR
MLBr_02065|M.leprae_Br4923 KEHGSEGNFVRTETIPEFLAALQTPRRVLIMVKAGDATDAVINELADVME
* . *: : * . :: .
Mb0773c|M.bovis_AF2122/97 PATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFM
Rv0751c|M.tuberculosis_H37Rv PATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFM
MMAR_1077|M.marinum_M SGALFIDSSTISVNDAREVHALAESHGMAQLDAPVSGGVKGAVAGTLAFM
MUL_0835|M.ulcerans_Agy99 SGALFIDSSTISVNDAREVHALAESHGMAQLDAPVSGGVKGAVAGTLAFM
MAV_4419|M.avium_104 PGALFIDSSTISVADAREVHAQAQSHGVAQLDAPVSGGVKGAVAGTLAFM
Mvan_1360|M.vanbaalenii_PYR-1 AGALLIDTSTISVDDARAIHDQATEKGLAQIDAPVSGGIKGATAGTLAFM
Mflv_5012|M.gilvum_PYR-GCK AGALLIDTSTISVDDARAIHEQATAGGLAQIDAPVSGGIKGATAGTLAFM
MSMEG_1496|M.smegmatis_MC2_155 TGTLFIDTSTISVDDARTVHTQALEKGHAQLDAPVSGGVKGATAGTLAFM
TH_0328|M.thermoresistible__bu SGALFIDTSTISVDDARTVHAQATGRGFAQVDAPVSGGVKGAVAGTLAFM
MAB_1186c|M.abscessus_ATCC_199 PGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSGGVPGATAATLTFM
MLBr_02065|M.leprae_Br4923 PSDIIIDGGNSLFTDTIRREKAMRERGLHFVGAGISGGEEGALNG-PSIM
.. ::** .. . *: . * .* :*** ** . ::*
Mb0773c|M.bovis_AF2122/97 VGGDESTLRRARPVLEPMAGKII------HCGAAGAGQAAKVCNNMVLAV
Rv0751c|M.tuberculosis_H37Rv VGGDESTLRRARPVLEPMAGKII------HCGAAGAGQAAKVCNNMVLAV
MMAR_1077|M.marinum_M VGGDQSALAQARPVLEPMAGKII------HCGAAGAGQAAKVCNNMVLAV
MUL_0835|M.ulcerans_Agy99 VGGDQSALAQARPVLEPMAGKII------HCGAAGAGQAAKVCNNMVLAV
MAV_4419|M.avium_104 VGGEPEALERARPVLEPMAGKII------HCGAAGAGQAAKVCNNMVLAV
Mvan_1360|M.vanbaalenii_PYR-1 VGGEDEAFERAKPVLEPMAGKII------HCGASGAGQAAKLCNNMVLAV
Mflv_5012|M.gilvum_PYR-GCK VGGDDEAFERAKPVLEPMAGKII------HCGASGAGQAAKLCNNMVLAV
MSMEG_1496|M.smegmatis_MC2_155 VGGEDDAVERARPILDPMAGKII------HCGGSGTGQAAKLCNNMVLAV
TH_0328|M.thermoresistible__bu VGGDAEAVERARPVLEPMAGKII------HCGASGTGQAAKLCNNMVLAV
MAB_1186c|M.abscessus_ATCC_199 VGADDAVFVAAEPILDAMGKRVV------HCGGPGVGQAAKICNNMILGV
MLBr_02065|M.leprae_Br4923 PGGPAESYTSLGPLLEEISAHVDGVSCCTHIGPGGSGHFVKMVHNGIEYS
*. *:*: :. :: * * * *: .*: :* :
Mb0773c|M.bovis_AF2122/97 QQIAIAEAFVLAEK-LGLSAQSLFDVITGATG---NCWAVHTNCPVPGPV
Rv0751c|M.tuberculosis_H37Rv QQIAIAEAFVLAEK-LGLSAQSLFDVITGATG---NCWAVHTNCPVPGPV
MMAR_1077|M.marinum_M QQIAIGEAFVLAEN-LGLDAQSLFDVITGATG---NCWAVHTNCPVPGPV
MUL_0835|M.ulcerans_Agy99 QQIAIGEAFVLAEN-LGLDAQSLFDVITGATG---NCWAVHTNCPVPGPV
MAV_4419|M.avium_104 QQIAIGEAFILAEK-LGLSAQSLFDVITGATG---NCWAVHTNCPVPGPV
Mvan_1360|M.vanbaalenii_PYR-1 QQIAIGEAFVLAEK-LGLPAQSLFDVITGATG---NCWAVHTNCPVPGPV
Mflv_5012|M.gilvum_PYR-GCK QQIAIGEAFVLAEK-LGLPAQSLFDVITGATG---NCWSVHTNCPVPGPV
MSMEG_1496|M.smegmatis_MC2_155 QQIAIGEAFVLAEK-LGLSAQSLFDVITGATG---NCWSVHTNCPVPGPV
TH_0328|M.thermoresistible__bu QQIAIGEAFVLAEK-LGLSAQSLFDVITGATG---NCWAVHTNCPVPGPV
MAB_1186c|M.abscessus_ATCC_199 SMIAISEAFVLGEK-LGLSHQALFDVAANASG---QCWALTSNCPVPGPV
MLBr_02065|M.leprae_Br4923 DMQLIGEAYQLLRDGLGMSAPQIADVFTEWNRGDLNSYLVEITAEVLRQT
. *.**: * .. **: : ** : . :.: : .. * .
Mb0773c|M.bovis_AF2122/97 PTSPAN----------NDFKPGFSTALMNKDLGLAMDAVAATGATAPLGS
Rv0751c|M.tuberculosis_H37Rv PTSPAN----------NDFKPGFSTALMNKDLGLAMDAVAATGATAPLGS
MMAR_1077|M.marinum_M PTSPAN----------NDFKPGFATALMNKDLGLAMDAVTSTGSAAPLGS
MUL_0835|M.ulcerans_Agy99 PTSPAN----------NDFKPGFATALMNKDLGLAMDAVTSTASAAPLGS
MAV_4419|M.avium_104 PTSPAN----------NDFKPGFAAALMNKDLGLAMDAVTSTGAAAPLGR
Mvan_1360|M.vanbaalenii_PYR-1 PTSPAN----------NDFKPGFATALMNKDLGLAMAAVSSTGSSAPLGT
Mflv_5012|M.gilvum_PYR-GCK PTSPAN----------NDFKPGFATALMNKDLGLAMAAVESSGAKAPLGT
MSMEG_1496|M.smegmatis_MC2_155 PTSPAN----------NDFKPGFATSLMNKDLGLAMAAVSSTGASAPLGS
TH_0328|M.thermoresistible__bu PTSPAN----------NDFKPGFATALMNKDLGLAMDAVKSSGASAPLGS
MAB_1186c|M.abscessus_ATCC_199 PTSPAN----------RDYAPGFAVALMAKDLGLAANAVRANGVDAQLGL
MLBr_02065|M.leprae_Br4923 DIKTGRPLVDVILDKAEQKGTGRWTVQSALDLGVPITGIAEAVFARALSG
.... .: .* . ***:. .: *.
Mb0773c|M.bovis_AF2122/97 HAADIYAKFA-----ADHADLDFSAVIHTLRARADA--------------
Rv0751c|M.tuberculosis_H37Rv HAADIYAKFA-----ADHADLDFSAVIHTLRARADA--------------
MMAR_1077|M.marinum_M HAAEIYAKFA-----ADHADQDFSAVIEMLRGS-----------------
MUL_0835|M.ulcerans_Agy99 HAAEIYAKFA-----TDHADQDFSAVIEMLRGS-----------------
MAV_4419|M.avium_104 HAAEIYAKFA-----ASHGDKDFSAVIEMLRGAC----------------
Mvan_1360|M.vanbaalenii_PYR-1 HAAEIYAEFN-----KEHADKDFSAIIEALRG------------------
Mflv_5012|M.gilvum_PYR-GCK HAAEIYSDFA-----KEHAGKDFSAIIEALRD------------------
MSMEG_1496|M.smegmatis_MC2_155 HAAEIYAKFN-----EEHADKDFSAVIELLRG------------------
TH_0328|M.thermoresistible__bu HAAEIYAEFIRAA--DGNADKDFSAVIELLRNS-----------------
MAB_1186c|M.abscessus_ATCC_199 AAAHIYDDFN--S--TGGSGLDFSAIFSRIQGAS----------------
MLBr_02065|M.leprae_Br4923 SVLQRKAAIGLASGKLGNKPTDRETFIEDVRQALYASKIVAYAQGFNHIQ
. . : * .:.: ::
Mb0773c|M.bovis_AF2122/97 --------------------------------------------------
Rv0751c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1077|M.marinum_M --------------------------------------------------
MUL_0835|M.ulcerans_Agy99 --------------------------------------------------
MAV_4419|M.avium_104 --------------------------------------------------
Mvan_1360|M.vanbaalenii_PYR-1 --------------------------------------------------
Mflv_5012|M.gilvum_PYR-GCK --------------------------------------------------
MSMEG_1496|M.smegmatis_MC2_155 --------------------------------------------------
TH_0328|M.thermoresistible__bu --------------------------------------------------
MAB_1186c|M.abscessus_ATCC_199 --------------------------------------------------
MLBr_02065|M.leprae_Br4923 TGSTEFGWNITPGDLATIWRGGCIIRAKFLNRIKEAFDAAPDLASLIVAP
Mb0773c|M.bovis_AF2122/97 --------------------------------------------------
Rv0751c|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_1077|M.marinum_M --------------------------------------------------
MUL_0835|M.ulcerans_Agy99 --------------------------------------------------
MAV_4419|M.avium_104 --------------------------------------------------
Mvan_1360|M.vanbaalenii_PYR-1 --------------------------------------------------
Mflv_5012|M.gilvum_PYR-GCK --------------------------------------------------
MSMEG_1496|M.smegmatis_MC2_155 --------------------------------------------------
TH_0328|M.thermoresistible__bu --------------------------------------------------
MAB_1186c|M.abscessus_ATCC_199 --------------------------------------------------
MLBr_02065|M.leprae_Br4923 YFRSAVESAIDSWRRVVSTATQLGIPNPGFSSALSYYDALRTERLPAALT
Mb0773c|M.bovis_AF2122/97 ----------------------------------
Rv0751c|M.tuberculosis_H37Rv ----------------------------------
MMAR_1077|M.marinum_M ----------------------------------
MUL_0835|M.ulcerans_Agy99 ----------------------------------
MAV_4419|M.avium_104 ----------------------------------
Mvan_1360|M.vanbaalenii_PYR-1 ----------------------------------
Mflv_5012|M.gilvum_PYR-GCK ----------------------------------
MSMEG_1496|M.smegmatis_MC2_155 ----------------------------------
TH_0328|M.thermoresistible__bu ----------------------------------
MAB_1186c|M.abscessus_ATCC_199 ----------------------------------
MLBr_02065|M.leprae_Br4923 QAQRDFFGAHSYGRVDARGKFHTLWSEDRSEVTV