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DTALGFLGLGNMGFPMMGRLLSAGHPVVVFDTDPAAVSRATALGADAAASVREVADRAGTVLASLPTPQV SNSVAEEVAGGSRVRRFVDLSTVGQHAAQRNSELLGPHGIAALDSPVSGGVHGAEAGTLAVMVSGPRHEF DALEPVWSAIGRATFVSEKAGAAQTMKLINNLIAATTLAVTAEAMVMGAKAGLDSQVMLDVLNAGSGGTH ASRDKFPRAVLPRTFDYGFATGLMTKDVLLYLSETQAMGSPSALAEVVARVWRDTQDEEGPASDFTSVVK PMERRAGVTLGPQ*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1911 | - | - | 100% (294) | 6-phosphogluconate dehydrogenase, NAD-binding |
| M. gilvum PYR-GCK | Mflv_5012 | - | 2e-37 | 32.99% (288) | 3-hydroxyisobutyrate dehydrogenase |
| M. gilvum PYR-GCK | Mflv_1602 | - | 4e-25 | 33.58% (265) | 6-phosphogluconate dehydrogenase, NAD-binding |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0773c | mmsB | 4e-37 | 34.80% (296) | 3-hydroxyisobutyrate dehydrogenase MmsB |
| M. tuberculosis H37Rv | Rv0751c | mmsB | 4e-37 | 34.80% (296) | 3-hydroxyisobutyrate dehydrogenase MmsB |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_1186c | - | 8e-33 | 33.80% (284) | 3-hydroxyisobutyrate dehydrogenase |
| M. marinum M | MMAR_1077 | mmsB | 2e-37 | 36.11% (288) | 3-hydroxyisobutyrate dehydrogenase, MmsB |
| M. avium 104 | MAV_0931 | - | 2e-96 | 61.51% (291) | 3-hydroxyisobutyrate dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_4123 | - | 1e-36 | 34.33% (300) | 3-hydroxyisobutyrate dehydrogenase |
| M. thermoresistible (build 8) | TH_0328 | mmsB | 2e-28 | 32.50% (280) | PROBABLE 3-HYDROXYISOBUTYRATE DEHYDROGENASE MMSB (HIBADH) |
| M. ulcerans Agy99 | MUL_0835 | mmsB | 3e-37 | 35.76% (288) | 3-hydroxyisobutyrate dehydrogenase, MmsB |
| M. vanbaalenii PYR-1 | Mvan_4822 | - | 1e-124 | 76.79% (293) | 3-hydroxyisobutyrate dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1911|M.gilvum_PYR-GCK --MDTALGFLGLGNMGFPMMGRLLS-AGHPVVVFDTDPA-AVSRATALGA
Mvan_4822|M.vanbaalenii_PYR-1 --MNTELGFVGLGNMGFPIMTRLVE-AGHRVLVFDTRAD-VVAQAVRAGA
MAV_0931|M.avium_104 ----MTIGFIGLGNMGFPMASRLIR-EQHDVVAFDSRRE-ALERLVAAGA
MMAR_1077|M.marinum_M MTKSCQIAFLGLGHMGAPMSANLIG-AGHTVRGFDPVPA-AASAAAAKGA
MUL_0835|M.ulcerans_Agy99 MTKSCQIAFLGLGHMGAPMSANLIG-AGHTVRGFDPVPA-AASATAAKGA
TH_0328|M.thermoresistible__bu --------------MGGPMAANLVA-AGHTVRGFDPVAE-SRAAAADKGV
Mb0773c|M.bovis_AF2122/97 ---MTTIAFLGLGNMGAPMSANLVG-AGHVVRGFDPAPT-AASGAAAHGV
Rv0751c|M.tuberculosis_H37Rv ---MTTIAFLGLGNMGAPMSANLVG-AGHVVRGFDPAPT-AASGAAAHGV
MAB_1186c|M.abscessus_ATCC_199 ---MTNIAFIGLGHMGLPMAVNLTK-AGHTVAAFDLSEQ-ARDAATQAGV
MSMEG_4123|M.smegmatis_MC2_155 ---MTHVAFVGVGTMAAPMVHRFLDGSGWRLHLVDTVADRTAPFRDHAQV
*. *: .: : .* .
Mflv_1911|M.gilvum_PYR-GCK DAAASVREVADRAGTVLASLPTPQVSNSVAEEVA---GGSRVRRFVDLST
Mvan_4822|M.vanbaalenii_PYR-1 EARTSARDVADRAETVLASLPTPQVSNSVASEVA---EGSRVRRFVDLST
MAV_0931|M.avium_104 RPASSPKEVADGAETVLASLPTPGASLEVATGTAGVIEGARVKRYVDLST
MMAR_1077|M.marinum_M TMFDSAAEAVAGADVVITMLPNGAVVKRCYAEVLP--AARSGALFIDSST
MUL_0835|M.ulcerans_Agy99 TMFDSAAEAVARADVVITMLPNGAVVKRCYAEVLP--AARSGALFIDSST
TH_0328|M.thermoresistible__bu QTFDSGAEAVAGAEVVITSLPNGDIVKQCYAEVLP--AATSGALFIDTST
Mb0773c|M.bovis_AF2122/97 AVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLA--AARPATLFIDSST
Rv0751c|M.tuberculosis_H37Rv AVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLA--AARPATLFIDSST
MAB_1186c|M.abscessus_ATCC_199 PLAESGAAAASTADVVITMLPKGAHVLAAYEDLLP--AARPGTLFIDSST
MSMEG_4123|M.smegmatis_MC2_155 SVHEQLTSLPQDIDFVLLCLPNAEALRTVASEIASR-RCLGNTVVVNLST
. *: **. :: **
Mflv_1911|M.gilvum_PYR-GCK VGQHAAQRNSELLGPHGIAALDSPVSGGVHGAEAGTLAVMVSGPRHEFDA
Mvan_4822|M.vanbaalenii_PYR-1 VGQQAAQRNREVLSARGIAALDSPVSGGVHGARAGTLAVMVSGPRPEFEA
MAV_0931|M.avium_104 VGSRTAMRIHDLLARRNIVAIDSPVSGGVGGAEQGTLALMVSGPRGEFDV
MMAR_1077|M.marinum_M ISVNDAREVHALAESHGMAQLDAPVSGGVKGAVAGTLAFMVGGDQSALAQ
MUL_0835|M.ulcerans_Agy99 ISVNDAREVHALAESHGMAQLDAPVSGGVKGAVAGTLAFMVGGDQSALAQ
TH_0328|M.thermoresistible__bu ISVDDARTVHAQATGRGFAQVDAPVSGGVKGAVAGTLAFMVGGDAEAVER
Mb0773c|M.bovis_AF2122/97 ISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFMVGGDESTLRR
Rv0751c|M.tuberculosis_H37Rv ISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFMVGGDESTLRR
MAB_1186c|M.abscessus_ATCC_199 VDVADARAAHEAATAAGHRFADAPVSGGVPGATAATLTFMVGADDAVFVA
MSMEG_4123|M.smegmatis_MC2_155 VGRQALTEAADALTHAGYGCLDAPVSGGAEGARTGSLTIMASGDPTTIEQ
:. . *:*****. ** .:*:.*... .
Mflv_1911|M.gilvum_PYR-GCK LEPVWSAIGRATFVSE-KAGAAQTMKLINNLIAATTLAVTAEAMVMGAKA
Mvan_4822|M.vanbaalenii_PYR-1 LASVFAVLGRAIFVSE-QPGAAQTMKLINNLMAATALAATAEVMVMGVKA
MAV_0931|M.avium_104 IRTTLEALGRPFYVGD-KPGAAQTMKLANNILAANVLAATAEVVVMGVKA
MMAR_1077|M.marinum_M ARPVLEPMAGKIIHCG-AAGAGQAAKVCNNMVLAVQQIAIGEAFVLAENL
MUL_0835|M.ulcerans_Agy99 ARPVLEPMAGKIIHCG-AAGAGQAAKVCNNMVLAVQQIAIGEAFVLAENL
TH_0328|M.thermoresistible__bu ARPVLEPMAGKIIHCG-ASGTGQAAKLCNNMVLAVQQIAIGEAFVLAEKL
Mb0773c|M.bovis_AF2122/97 ARPVLEPMAGKIIHCG-AAGAGQAAKVCNNMVLAVQQIAIAEAFVLAEKL
Rv0751c|M.tuberculosis_H37Rv ARPVLEPMAGKIIHCG-AAGAGQAAKVCNNMVLAVQQIAIAEAFVLAEKL
MAB_1186c|M.abscessus_ATCC_199 AEPILDAMGKRVVHCG-GPGVGQAAKICNNMILGVSMIAISEAFVLGEKL
MSMEG_4123|M.smegmatis_MC2_155 CSSVFDVIAGKVIVVGNQPGQAQAIKVANNVLSLGALVATAEATAITRKA
. :. .* .*: *: **:: . .*. .: :
Mflv_1911|M.gilvum_PYR-GCK GLDSQVMLDVLNAGSGGTHASRD------KFPRAVLPRTFDYGFATGLMT
Mvan_4822|M.vanbaalenii_PYR-1 GLDPQVVIDVLNAGSGGTHASRD------KFPRAVLPRTFDYGFATGLMT
MAV_0931|M.avium_104 GLDPAVMIEVLNAGSGATSASRD------KFPRAILPRSFDYGFATGLMV
MMAR_1077|M.marinum_M GLDAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFATALMN
MUL_0835|M.ulcerans_Agy99 GLDAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFATALMN
TH_0328|M.thermoresistible__bu GLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFATALMN
Mb0773c|M.bovis_AF2122/97 GLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTALMN
Rv0751c|M.tuberculosis_H37Rv GLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTALMN
MAB_1186c|M.abscessus_ATCC_199 GLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVALMA
MSMEG_4123|M.smegmatis_MC2_155 GIPLDTALEALNASSGRNTATAV------KFPRHVLTGRFDFGFPTSGAL
*: ::. .:* * .* : **...
Mflv_1911|M.gilvum_PYR-GCK KDVLLYLSETQAMGSPSALAEVVARVWRDTQ--DEEGPASDFTSVVKPME
Mvan_4822|M.vanbaalenii_PYR-1 KDVLLYLDEARALGTPVALAESVMRLWEQTQ--DEEGPSSDFTSVVKPLE
MAV_0931|M.avium_104 KDVRLYLDEAKALGVPAEVAETIARLWETAA--REEGPEADFTTVIKPLE
MMAR_1077|M.marinum_M KDLGLAMDAVTSTGSAAPLGSHAAEIYAKF---AADHADQDFSAVIEMLR
MUL_0835|M.ulcerans_Agy99 KDLGLAMDAVTSTASAAPLGSHAAEIYAKF---ATDHADQDFSAVIEMLR
TH_0328|M.thermoresistible__bu KDLGLAMDAVKSSGASAPLGSHAAEIYAEFIRAADGNADKDFSAVIELLR
Mb0773c|M.bovis_AF2122/97 KDLGLAMDAVAATGATAPLGSHAADIYAKF---AADHADLDFSAVIHTLR
Rv0751c|M.tuberculosis_H37Rv KDLGLAMDAVAATGATAPLGSHAADIYAKF---AADHADLDFSAVIHTLR
MAB_1186c|M.abscessus_ATCC_199 KDLGLAANAVRANGVDAQLGLAAAHIYDDFN--STGGSGLDFSAIFSRIQ
MSMEG_4123|M.smegmatis_MC2_155 KDVSLFLELAAELGVEAPLSSAVQAIWHTAA--EQGFGQQDCTRIVSFYE
**: * . . . :. :: * : :. .
Mflv_1911|M.gilvum_PYR-GCK RRAGVTLGPQ--
Mvan_4822|M.vanbaalenii_PYR-1 RIAGVTIGPT--
MAV_0931|M.avium_104 RAAGVTVGRSAR
MMAR_1077|M.marinum_M GS----------
MUL_0835|M.ulcerans_Agy99 GS----------
TH_0328|M.thermoresistible__bu NS----------
Mb0773c|M.bovis_AF2122/97 ARADA-------
Rv0751c|M.tuberculosis_H37Rv ARADA-------
MAB_1186c|M.abscessus_ATCC_199 GAS---------
MSMEG_4123|M.smegmatis_MC2_155 ARAGLSEGQPV-