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LPGDTRDDAALRKSRGAFFTPPPVARFLVDWAVRDPGHAVLEPSCGEAVFLHEIGRRGDGGRPHTGPLVG VEIHPGSAAAAEQSLRAAGVGATIHARDFFGHNEFGRYDAVVGNPPYVRYQDFAGESRARAREAALRAGV TLSNLASSWAAFTVHSALHLRTGGRLGLVLPAELLSVNYAAGVRQFLMDHFSSVGLVLFDERVFPGVLEE VVLLLADGYDAAGGNGATHMQLSQVRDAEGLARLTASRRWTPPAGGAKWSAGLLSSAGLSAFDAAVGSDA FTVLETWGDTTLGMVTGSNRFFTLSPAKVAALGLAPSDVLSLSPPGSRHLRGLGLTPQALHTLGADGRST FLFRPKGEPSPAAWHYIRSGEDLDVHQAYKCRVRKPWWRVPYLRPADLFLTYMNADTPRVTSNRAQAHHL NSVHGVYLRDDLRRDGMSLLPLAALNSVTLLGAETVGRAYGGGMLKIEPREADVLPVPSPELVRRNADGL QAVRSAVAARLRDGRLLDAVALVDDALLLSTSSLTVRQLRALRRDHGALTARRVARGKANGGR*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1345 | - | - | 100% (554) | N-6 DNA methylase |
| M. gilvum PYR-GCK | Mflv_2413 | - | 1e-06 | 25.64% (195) | N-6 DNA methylase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3291 | - | 2e-70 | 35.16% (546) | DNA methylase (modification methylase) (methyltransferase) |
| M. tuberculosis H37Rv | Rv3263 | - | 2e-70 | 35.16% (546) | DNA methylase (modification methylase) (methyltransferase) |
| M. leprae Br4923 | MLBr_00756 | - | 5e-68 | 34.00% (547) | putative DNA methylase |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_1279 | - | 7e-77 | 36.40% (544) | DNA methylase |
| M. avium 104 | MAV_4226 | - | 6e-76 | 36.61% (549) | BpmI methyltransferase |
| M. smegmatis MC2 155 | MSMEG_3213 | - | 1e-156 | 55.58% (538) | hypothetical protein MSMEG_3213 |
| M. thermoresistible (build 8) | - | - | - | - | - |
| M. ulcerans Agy99 | MUL_2608 | - | 2e-76 | 36.21% (544) | DNA methylase |
| M. vanbaalenii PYR-1 | Mvan_5445 | - | 0.0 | 81.47% (556) | methyltransferase small |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3291|M.bovis_AF2122/97 MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVA
Rv3263|M.tuberculosis_H37Rv MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVA
MLBr_00756|M.leprae_Br4923 ----------MVITYIGAAPDTCSMTTFAGKTAASADKVRGGYYTPAPVA
MMAR_1279|M.marinum_M ------------------------MKTFAGKTAASADKVRGGYYTPAPVA
MUL_2608|M.ulcerans_Agy99 ------------------------MKTFAGKTAASADKVRGGYYTPAPVV
MAV_4226|M.avium_104 ------------------------MTTFAGKAAASADKVRGGYYTPAPVA
Mflv_1345|M.gilvum_PYR-GCK ------------------MLPGDTRDD------AALRKSRGAFFTPPPVA
Mvan_5445|M.vanbaalenii_PYR-1 ----------------MRMLPGDN---------ADLRKARGAFFTPTPVA
MSMEG_3213|M.smegmatis_MC2_155 ----------MRRGVAVRGLVGGVVNNDGVTETAQDRKARGAFFTPDAIT
* * **.::** .:.
Mb3291|M.bovis_AF2122/97 RFLAHWVHQAG-PKILEPSCGDGRILRELSAITD-------HAHGVELVA
Rv3263|M.tuberculosis_H37Rv RFLAHWVHQAG-PKILEPSCGDGRILRELSAITD-------HAHGVELVA
MLBr_00756|M.leprae_Br4923 RFLARWVRQAG-QKILEPSCGDGRILREIAALTT-------QAHGVELLA
MMAR_1279|M.marinum_M RFLARWVGRAG-TKILEPSCGDGRILAELAAASG-------SARGVELIA
MUL_2608|M.ulcerans_Agy99 RFLARWVGRAG-TKILEPSCGDGRILAELAAASG-------SARGVELIA
MAV_4226|M.avium_104 RFLSRWVRRAG-PRIVEPSCGDGAILRELARLSD-------RVHGVELIP
Mflv_1345|M.gilvum_PYR-GCK RFLVDWAVRDPGHAVLEPSCGEAVFLHEIGRRGDGGRPHTGPLVGVEIHP
Mvan_5445|M.vanbaalenii_PYR-1 RFLTDWAVRHPEHSVLEPSCGEAVFLHEIGQRGG----HTGPLVGVEIHP
MSMEG_3213|M.smegmatis_MC2_155 TYLTQWAVRSDADRVFEPSAGDAAFLVAAIRRLQALGVDHPTVDGVEIHA
:* *. : :.***.*:. :* ***: .
Mb3291|M.bovis_AF2122/97 REAKKSRD----FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWAS
Rv3263|M.tuberculosis_H37Rv REAKKSRD----FASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWAS
MLBr_00756|M.leprae_Br4923 HEARKSRK----FCSVDTENFFTWLSKTQVGSWDGVAGNPPYIRFGNWAA
MMAR_1279|M.marinum_M DEAAKARR----FAPVDHTNLFTWLDESATGRWDGVAGNPPYIRFGNWPA
MUL_2608|M.ulcerans_Agy99 DEAAKARR----FAPVDNTNLFTWLDESATGRWDGVAGNPPYIRFGNWPA
MAV_4226|M.avium_104 HEAAKARR----FAPVSAESLFSWLATAEDGGWDGVAGNPPYIRFGNWAS
Mflv_1345|M.gilvum_PYR-GCK GSAAAAEQSLRAAGVGATIHARDFFGHNEFGRYDAVVGNPPYVRYQDFAG
Mvan_5445|M.vanbaalenii_PYR-1 GSAEESQRSLRARGIDATIHARDFFSHAEFGSYDAVVGNPPYVRYQDFAG
MSMEG_3213|M.smegmatis_MC2_155 ASAASARRRVADAGGRARIRTANFFAVDPRPEYTAVIGNPPYIRYQDFRG
.* :. :: : .* *****:*: :: .
Mb3291|M.bovis_AF2122/97 EQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELL
Rv3263|M.tuberculosis_H37Rv EQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVGLVVPAELL
MLBr_00756|M.leprae_Br4923 DQRDPALELMRHVGLRPTKLTNAWVPFVVASTTLVRDGGRVGLVVPAELL
MMAR_1279|M.marinum_M DQRDPALELMRQVGLNPTRLTNAWVPFVVASTIVVRDGGRVGLVLPAELL
MUL_2608|M.ulcerans_Agy99 DQRDPALELMRQVGLNPTRLTNAWVPFVVASTIVVRDGGRVGLVLPAELL
MAV_4226|M.avium_104 PQRDPALALLRREGLRPTRLTNAWVPFVVAGTVLVRDGGRVGLVLPAELL
Mflv_1345|M.gilvum_PYR-GCK ESRARAREAALRAGVTLSNLASSWAAFTVHSALHLRTGGRLGLVLPAELL
Mvan_5445|M.vanbaalenii_PYR-1 EARARARQAALRAGVALSNLASSWAAFTVHSALHLRPGGRLGLVLPAELL
MSMEG_3213|M.smegmatis_MC2_155 QTRAESRRAALRAGVTLSGLASSWAAFTVHAALFLRPGGRMALVLPAELL
* : : *: : *:.:*..*.* .: * ***:.**:*****
Mb3291|M.bovis_AF2122/97 QVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHI
Rv3263|M.tuberculosis_H37Rv QVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHI
MLBr_00756|M.leprae_Br4923 QVTYAAQLREFLLSLYREIILVTFERLVFDGILQEVVLFCGVVGAGPAQI
MMAR_1279|M.marinum_M QVGYAGQLREFLLNRFREITLVTFDRLVFDGVLQEVVLFCGVVGSDAAQI
MUL_2608|M.ulcerans_Agy99 QVGYAGQLREFLLNRFREITLVTFDRLVFDGVLQEVVLFCSDVGSDAAQI
MAV_4226|M.avium_104 QVGYAAQLREFLLSRFRDITLLTFERLVFDGVLQEVVLFCGVVGTGPARI
Mflv_1345|M.gilvum_PYR-GCK SVNYAAGVRQFLMDHFSSVGLVLFDERVFPGVLEEVVLLLADGYDAAGGN
Mvan_5445|M.vanbaalenii_PYR-1 SVNYAAGVRQYLMDHFGQVGLVLFDERVFPGVLEEVVLLLADGYQPDGGR
MSMEG_3213|M.smegmatis_MC2_155 SVNYASAVRKFLFDRFASVELVMFDEQVFPEAEADVVLLLADGFD---GG
.* **. :*::*:. : .: *: *:. ** :***: .
Mb3291|M.bovis_AF2122/97 RTVRLGDANDLNALGDKDFT-NESAPALLHEKEKWTKYFLDPAQIRLLRG
Rv3263|M.tuberculosis_H37Rv RTVRLGDANDLNALGDKDFT-NESAPALLHEKEKWTKYFLDPAQIRLLRG
MLBr_00756|M.leprae_Br4923 RTINLRDAESLNSLAESNLG-VESAPALLHEKEKWTKYLLDPAQIRLLRK
MMAR_1279|M.marinum_M RTVNLGDAD---ALATADLE-VEPAAALLHENEKWTKYFLDPRQIQTMRA
MUL_2608|M.ulcerans_Agy99 RTVNLGDAD---ALATADLE-VEPAAALRHENEKWTKYFLDPRQIQTMRA
MAV_4226|M.avium_104 RTVTLAGAD---ALADIDVERLASAPALLHEHEKWTKYFLDPAAIELLRA
Mflv_1345|M.gilvum_PYR-GCK GATHMQLSQVRDAEGLARLT-ASRRWTPPAGGAKWSAGLLSSAGLSAFDA
Mvan_5445|M.vanbaalenii_PYR-1 GATHMRLSQVRDAAGLARLT-GSRRWSPPGNGAKWSAGLMSAAGLSAFDS
MSMEG_3213|M.smegmatis_MC2_155 PSGHAVIHRARNAEALTSEL-VQRRWRPRDPAEKWISGLIGSAPVEVLHG
: : . ** ::.. : :
Mb3291|M.bovis_AF2122/97 LKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSR
Rv3263|M.tuberculosis_H37Rv LKQSATMIRLGELADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSR
MLBr_00756|M.leprae_Br4923 LKHANAMTRFGEVADVDVGIVTGRNSFFTFTDADAHALKLRKHCVPLVSR
MMAR_1279|M.marinum_M LKQCDAMIRLGAVADVNVGIVTGRNSFFTFTDTVARQLGLAAHCVPLVSR
MUL_2608|M.ulcerans_Agy99 LKQCDAMIRLGAVADVNVGIVTGRNSFFTFTDTVARQLGLAAHCVPLVSR
MAV_4226|M.avium_104 LKASATLTRLGALAEVDVGIVTGRNAFFTFTDEQVTALGLRRHCVPLVSR
Mflv_1345|M.gilvum_PYR-GCK AVGSDAFTVLETWGDTTLGMVTGSNRFFTLSPAKVAALGLAPSDVLSLSP
Mvan_5445|M.vanbaalenii_PYR-1 VVRGDAFTFLETWGDTTLGMVTGSNRFFTLSPDKVDALGLAPSDLIPLSP
MSMEG_3213|M.smegmatis_MC2_155 LEAAGDFTCLETWADTTLGMVTGNNGYFALSPQRVHELGLRQADLLPLSP
: : .:. :*:*** * :*::: . * * : :*
Mb3291|M.bovis_AF2122/97 --SAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAA
Rv3263|M.tuberculosis_H37Rv --SAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAA
MLBr_00756|M.leprae_Br4923 --STQLAGIVYDTNCRANDIAAKHRTWLLDAPHHPTDSALVTHIDAGKRA
MMAR_1279|M.marinum_M --SAQLRGLVYDPDCRASDIAADHRTWLLDAPADPADPALIAHIDAGEAA
MUL_2608|M.ulcerans_Agy99 --SAQLRGLVYDPDCRASDIAADHRTWLLDAPADPADPALIAHIDAGEAA
MAV_4226|M.avium_104 --SAQLSGLIYDTDCRASDLAAGQRGWLLNAPRKPADPALTAHIRAGEAA
Mflv_1345|M.gilvum_PYR-GCK PGSRHLRGLGLTPQALHTLGADGRSTFLFRPK-GEPSPAAWHYIRSGEDL
Mvan_5445|M.vanbaalenii_PYR-1 PGSRHLRGLALTPKALRALGEEGRSTYLFRPA-DEPSPAGWHYIASGEDL
MSMEG_3213|M.smegmatis_MC2_155 PGSAHLRGLTLSADQLTRLGEDGRSVHLFRPTGAKLSAAAARYVEAGHAA
* :* *: . : *: . ..* :: :*.
Mb3291|M.bovis_AF2122/97 GVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTV
Rv3263|M.tuberculosis_H37Rv GVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTV
MLBr_00756|M.leprae_Br4923 GVDKGYKCSIRKPWWSTPSLWIPDLFMLRQIHLAPRLTVNAAAATSTDTV
MMAR_1279|M.marinum_M GVHLGYKCSIRKPWWSTPSLWVPDLFMLRQIHRTPRLSVNSAAATSTDTV
MUL_2608|M.ulcerans_Agy99 GVHLGYKCSIRKPWWSTPSLWVPDLFMLRQIHRTPRLSVNSAAATSTDTV
MAV_4226|M.avium_104 GVHRGYKCSIRTPWWSTPSLWQPDLFLLRQIHAAPRLTVNAAGATSTDTV
Mflv_1345|M.gilvum_PYR-GCK DVHQAYKCRVRKPWWRVPYLRPADLFLTYMNADTPRVTSNRAQAHHLNSV
Mvan_5445|M.vanbaalenii_PYR-1 DVHRAYKCRVRTPWWRVPYLRPADLFLTYMNADTPRLTSNRARTHHLNSV
MSMEG_3213|M.smegmatis_MC2_155 GVHLAYKCRVRTPWYLVPLVEPADLLLTCMNADTPRLVTNRARAHHLNSV
.*. .*** :*.**: .* : .**:: :**: * * : ::*
Mb3291|M.bovis_AF2122/97 HRVRLDPN---VDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAE
Rv3263|M.tuberculosis_H37Rv HRVRLDPN---VDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPREAE
MLBr_00756|M.leprae_Br4923 HRVRLIAD---VDPTALAAVFHNSATFAFAEIMGRSYGGGILELEPREAE
MMAR_1279|M.marinum_M HRVRIKTAGPRVDPTALAVAFHNSVTFAFAEIMGRSYGGGILELEPREAE
MUL_2608|M.ulcerans_Agy99 HRVRIKTAGPRVDPTALAVAFHNSVTFAFAEIMDRSYGGGILELEPREAE
MAV_4226|M.avium_104 HRVRVGPG---VDPAALAAVFHNSVTFAFAEIMGRSYGGGVLELEPREAE
Mflv_1345|M.gilvum_PYR-GCK HGVYLRDDLRRDGMSLLPLAALNSVTLLGAETVGRAYGGGMLKIEPREAD
Mvan_5445|M.vanbaalenii_PYR-1 HGVYLRDEFRQDGMNLLPLASLNSVTLLGAETVGRAYGGGMLKIEPREAD
MSMEG_3213|M.smegmatis_MC2_155 HGVYLRDGFTTLGRDLLPLASLNSVTLLHAEMVGRSYGGGILKIEPREAD
* * : . *. . **.*: ** :.*:****:*::*****:
Mb3291|M.bovis_AF2122/97 QLPMPPP------AYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLG
Rv3263|M.tuberculosis_H37Rv QLPMPPP------AYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLG
MLBr_00756|M.leprae_Br4923 QLPIPPP------AYASTELGQDIDLLLKANEIEKALDIVDRRVLIDGLG
MMAR_1279|M.marinum_M LLPIPPP------TRAGAELATDVDLLLKADEIDKALDLVDRHVLIGELG
MUL_2608|M.ulcerans_Agy99 LLPIPPP------TRAGAELATDVDLLLKADEIDKALDLVDRHVLIGELG
MAV_4226|M.avium_104 QLPLPRP------ECADADLVGDVDLLLKAGELDKALDVVDRRVLIDALG
Mflv_1345|M.gilvum_PYR-GCK VLPVPSPELVRRNADGLQAVRSAVAARLRDGRLLDAVALVDDALLLSTSS
Mvan_5445|M.vanbaalenii_PYR-1 VLPVPSPDLVRRNADQLRRIRPAVATCLRDARLLDAVALVDEVLLLGTST
MSMEG_3213|M.smegmatis_MC2_155 QWLVPSPQLIETHAAPLRAVRRRVAGLLDRGRLLDAVATVDQALGVESG-
:* * : : * .: .*: ** : :
Mb3291|M.bovis_AF2122/97 LSPRLVAGCRAAWLTLRDRRTKRGSRR------------
Rv3263|M.tuberculosis_H37Rv LSPRLVAGCRAAWLTLRDRRTKRGSRR------------
MLBr_00756|M.leprae_Br4923 LSPDLVAQCRTAWASLRDRRTKRGSRVNISRRPFDSPPS
MMAR_1279|M.marinum_M MAPDAVARCRSAWMTLRDRRIKRGSR-------------
MUL_2608|M.ulcerans_Agy99 MAPDAVARCRSAWMTLRDRRIKRGSR-------------
MAV_4226|M.avium_104 VPPRAVADCRAAWACLRDRRKRRASR-------------
Mflv_1345|M.gilvum_PYR-GCK LTVRQLRALRRDHGALTARRVAR---GKANGGR------
Mvan_5445|M.vanbaalenii_PYR-1 LSERALRAVRRDHAGLTARRITRGKVGKAHGGE------
MSMEG_3213|M.smegmatis_MC2_155 ---AALESVRNAHHSLATRRTVR-----SRRAR------
: * * ** *