For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
PHDLAALAEQSGTRFILALFVDLRGKPCAKLVPVEAVDQLATEGVGFAGYAVGAMGQEPKDPDLMAIPDP ESFTPIPFIKEGLAIVHCDPHVNGKPWPYAPRVILKSLIQRCADAGFEPWVGAEVEYFLLRRNDDGSVSV ADAADTASQPCYDARGVTRMYDHLTSVSAAMNQLGWSNYANDHEDGNGQFEQNFKFAEALTTADRVITLR YLLSMIADERGMIATFMPKPFGDKTGNGLHFHLSLTSAGTPVFPAEDDNRGLGLSDTAYGFIGGILEHAC ALQAVVGPTVNSYKRTGAVATLSGASWAPRLPTYGGNDRTHYIRVPDGDRIEMRGGDGSANPYLAIAAAL GAGLDGIKRSTDPGAVGQGVSTQPLPATLLHAVEAFETNPVVTGVLDAAGEGVASYFAAIKRDEFFAYHS SVSPWEIDYYLTAF*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1300 | - | - | 100% (435) | glutamate--ammonia ligase |
| M. gilvum PYR-GCK | Mflv_2909 | - | 2e-49 | 32.00% (450) | glutamine synthetase, type I |
| M. gilvum PYR-GCK | Mflv_2928 | - | 6e-35 | 31.42% (401) | glutamine synthetase, type I |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2246c | glnA2 | 1e-52 | 32.44% (450) | glutamine synthetase |
| M. tuberculosis H37Rv | Rv2222c | glnA2 | 1e-52 | 32.44% (450) | glutamine synthetase |
| M. leprae Br4923 | MLBr_01631 | glnA2 | 3e-50 | 31.63% (449) | glutamine synthase class II |
| M. abscessus ATCC 19977 | MAB_1920 | - | 1e-52 | 33.41% (449) | glutamine synthetase |
| M. marinum M | MMAR_3294 | glnA2 | 1e-51 | 32.44% (450) | glutamine synthetase GlnA2 |
| M. avium 104 | MAV_2244 | glnA | 6e-54 | 32.89% (450) | glutamine synthetase, type I |
| M. smegmatis MC2 155 | MSMEG_6260 | glnT | 0.0 | 83.68% (435) | glutamine synthetase, type III |
| M. thermoresistible (build 8) | TH_3396 | - | 1e-52 | 32.67% (450) | PUTATIVE glutamine synthetase, type I |
| M. ulcerans Agy99 | MUL_1335 | glnA2 | 5e-52 | 32.67% (450) | glutamine synthetase GlnA2 |
| M. vanbaalenii PYR-1 | Mvan_5509 | - | 0.0 | 89.43% (435) | glutamate--ammonia ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb2246c|M.bovis_AF2122/97 MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
Rv2222c|M.tuberculosis_H37Rv MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
MMAR_3294|M.marinum_M MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
MUL_1335|M.ulcerans_Agy99 MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
MAV_2244|M.avium_104 MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
MLBr_01631|M.leprae_Br4923 MDRQKEFVLRTLEERDIRFVRLWFTDVLGYLKSVAIAPAELEGAFEEGIG
TH_3396|M.thermoresistible__bu MDRQKEFVLRSLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIG
MAB_1920|M.abscessus_ATCC_1997 MDRQKEFVLRTLEERDIRFVRLWFTDVLGYLKSVSIAPAELEGAFEEGIG
Mflv_1300|M.gilvum_PYR-GCK ---MPHDLAALAEQSGTRFILALFVDLRGKPCAKLVPVEAVDQLATEGVG
Mvan_5509|M.vanbaalenii_PYR-1 ---MAEDLATLAEQHGTKFILALFVDLRGKPCAKLVPVEAVDLLSTEGVG
MSMEG_6260|M.smegmatis_MC2_155 ---MTSDLATLAEQSGTKFILALFVDLRGKPCAKLVPVEAIDMLATEGVG
: *: . :*: *.*: * : :. :: **:*
Mb2246c|M.bovis_AF2122/97 FDGSSIEGFARVS-ESDTVAHPDPSTFQVLPWATSSGHHHSARMFCDITM
Rv2222c|M.tuberculosis_H37Rv FDGSSIEGFARVS-ESDTVAHPDPSTFQVLPWATSSGHHHSARMFCDITM
MMAR_3294|M.marinum_M FDGSSIEGFARVS-ESDTVAHPDPSTFQVLPWVTDAGHHHSARIFCDITM
MUL_1335|M.ulcerans_Agy99 FDGSSIEGFARVS-ESDTVAHPDPSTFQVLPWVTDAGHHHSARIFCDITM
MAV_2244|M.avium_104 FDGSSIEGFSRVS-ESDTVANPDPSTFQVLPWASPNGHHHSARMFCDITM
MLBr_01631|M.leprae_Br4923 FDGSSIEGFARVS-ESDTVAHPDPSTFQILPWATTAGHHHSARMFCDITM
TH_3396|M.thermoresistible__bu FDGSAIEGFARVS-EADMVARPDPSTFQVLPWTGDSGKHYSARMFCDITM
MAB_1920|M.abscessus_ATCC_1997 FDGSSIEGFARVS-ESDTVAHPDPSTFQVLPWTTSAGKHHSARMFCDIKL
Mflv_1300|M.gilvum_PYR-GCK FAGYAVGAMGQEPKDPDLMAIPDPESFTPIPFIKEG----LAIVHCDPHV
Mvan_5509|M.vanbaalenii_PYR-1 FAGYAVGAMGQEPKDPDLMAIPDPASFTPIPFIKEG----LAIVHCDPHV
MSMEG_6260|M.smegmatis_MC2_155 FAGYAVGAMGQEPKDPDLMALPDPASFTPIPFIKEG----LALVHCDPYV
* * :: .:.: . :.* :* *** :* :*: * :.** :
Mb2246c|M.bovis_AF2122/97 PDGSPSWADPRHVLRRQLTKAGELGFSCYVHPEIEFFLLKPGPEDG--SV
Rv2222c|M.tuberculosis_H37Rv PDGSPSWADPRHVLRRQLTKAGELGFSCYVHPEIEFFLLKPGPEDG--SV
MMAR_3294|M.marinum_M PDGSPSWADPRHVLRRQLTKANELGFSCYVHPEIEFFLLNPGPEDG--TE
MUL_1335|M.ulcerans_Agy99 PDGSPSWADPRHVLRRQLIKANELGFSCYVHPEIEFFLLNPGPEDG--TE
MAV_2244|M.avium_104 PDGSPSWADPRHVLRRQLQKANDLGFSCYVHPEIEFFLLKPGPDDG--TP
MLBr_01631|M.leprae_Br4923 PDGSSSWADPRHVLRRQLTKANDLGFSCYVHPEIEFFLLKAGPEDGSMPI
TH_3396|M.thermoresistible__bu PDGSPSWADPRHVLRRQLAKAGDQGFTCYVHPEIEFFLLKPGPHDG--SP
MAB_1920|M.abscessus_ATCC_1997 PDGSPSWSDPRHVLRRQLAKASDLGFSCYVHPEIEFFLLKNLPDDG--TA
Mflv_1300|M.gilvum_PYR-GCK N-GKPWPYAPRVILKSLIQRCADAGFEPWVGAEVEYFLLRRNDDGSVSVA
Mvan_5509|M.vanbaalenii_PYR-1 N-GEPWPFAPRVILKSLIERCSDAGFEPWVGAEVEYFLLSRNDDGSIAVA
MSMEG_6260|M.smegmatis_MC2_155 E-GKPWPYAPRVILKNLIQQAADAGFEPWVGAEVEYFLLRRNADGSLVTA
*.. ** :*: : :. : ** :* .*:*:*** ...
Mb2246c|M.bovis_AF2122/97 PVPVDNAG-YFDQAVHDSALNFRRHAIDALEFMGISVEFSHHEGAPGQQE
Rv2222c|M.tuberculosis_H37Rv PVPVDNAG-YFDQAVHDSALNFRRHAIDALEFMGISVEFSHHEGAPGQQE
MMAR_3294|M.marinum_M PVPIDNAG-YFDQAVHVSASKFRRHAIDALEFMGISVEFSHHEGAPGQQE
MUL_1335|M.ulcerans_Agy99 PVPIDNAG-YFDQAVHVSASKFRRHVIDALEFMGISVEFSHHEGAPGQQE
MAV_2244|M.avium_104 PVPVDTAG-YFDQAIHDSASNFRRHAIEALEFMGISVEFSHHEGAPGQQE
MLBr_01631|M.leprae_Br4923 PVPVDNAG-YFDQAVHDSALNFRRHAIEALEFMGISVEFSHHEGAPGQQE
TH_3396|M.thermoresistible__bu PVPADTSG-YFDQSVHDTAPNFRRHAIEALEQMGISVEFSHHEGAPGQQE
MAB_1920|M.abscessus_ATCC_1997 PIPADDAG-YFDQAVHEAAPNFRRHAIDALESMGISVEFSHHEGAPGQQE
Mflv_1300|M.gilvum_PYR-GCK DAADTASQPCYDARGVTRMYDHLTSVSAAMNQLGWSNYANDHEDGNGQFE
Mvan_5509|M.vanbaalenii_PYR-1 DTADTAAQPCYDARGVTRMYDHLAAVSTAMNQLGWSNYANDHEDGNGQFE
MSMEG_6260|M.smegmatis_MC2_155 DAADTAAQPCYDARGLTRMYDHLTAISTAMNSLGWSNYANDHEDGNGQFE
. : :* .. *:: :* * ..**.. ** *
Mb2246c|M.bovis_AF2122/97 IDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHPGSAMHT
Rv2222c|M.tuberculosis_H37Rv IDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHPGSAMHT
MMAR_3294|M.marinum_M IDLRFADALSMADNVMTFRYVIKEVALEEGVRATFMPKPFGQHAGSAMHT
MUL_1335|M.ulcerans_Agy99 IDLRFADALSMADNVMTFRYVIKEVALEEGVRATFMPKPFGQHAGSAMHT
MAV_2244|M.avium_104 IDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHPGSAMHT
MLBr_01631|M.leprae_Br4923 IDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFAQHPGSAMHT
TH_3396|M.thermoresistible__bu IDLRYADALSMADNVMTFRYVVKQVAISDGVRASFMPKPFAEHPGSAMHT
MAB_1920|M.abscessus_ATCC_1997 IDLRYADALSMADNVMTFRNVVKEVAIIDGVHATFMPKPFSDHPGSAMHT
Mflv_1300|M.gilvum_PYR-GCK QNFKFAEALTTADRVITLRYLLSMIADERGMIATFMPKPFGDKTGNGLHF
Mvan_5509|M.vanbaalenii_PYR-1 QNFKFADALTTADRVITLRYLLSMIAAERGMIATFMPKPFGDKTGNGLHF
MSMEG_6260|M.smegmatis_MC2_155 QNFEFADALTTADRVITLRYLLSMIAAERGMVATFMPKPFADRTGSGLHF
::.:*:**: **.*:*:* ::. :* * *:******.::.*..:*
Mb2246c|M.bovis_AF2122/97 HMSLFEGDVNAFHSADDP--LQLSEVGKSFIAGILEHACEISAVTNQWVN
Rv2222c|M.tuberculosis_H37Rv HMSLFEGDVNAFHSADDP--LQLSEVGKSFIAGILEHACEISAVTNQWVN
MMAR_3294|M.marinum_M HMSLFEGDVNAFHSPDDP--LQLSEVGKSFIAGILEHASEISAVTNQWVN
MUL_1335|M.ulcerans_Agy99 HMSLFEGDVNAFHSPDDP--LQLSEVGKSFIAGILEHASEISAVTNQWVN
MAV_2244|M.avium_104 HMSLFEGDVNAFHSPDDP--LQLSDVGKSFIAGILEHASEISAVTNQWVN
MLBr_01631|M.leprae_Br4923 HMSLFEGDVNAFHSDDDP--LQLSDVGKSFIAGILEHASEISAVTNQWVN
TH_3396|M.thermoresistible__bu HMSLFEGDTNAFHSPDDP--LQLSDVAKSFIAGILEHANEISAVTNQWVN
MAB_1920|M.abscessus_ATCC_1997 HMSLFEGDTNAFHNPDDP--LQLSDVGKSFIAGILEHANEISAVTNQWVN
Mflv_1300|M.gilvum_PYR-GCK HLSLTSAGTPVFPAEDDNRGLGLSDTAYGFIGGILEHACALQAVVGPTVN
Mvan_5509|M.vanbaalenii_PYR-1 HLSLTSGGAPVFPAEEDERGLGLSDTAYGFIGGILEHACALQAVVAPTVN
MSMEG_6260|M.smegmatis_MC2_155 HLSLTSGGNPVFPSETDERGLGLSDTAYAFLAGILDHACALQSVVAPTVN
*:** ... .* * * **:.. .*:.***:** :.:*. **
Mb2246c|M.bovis_AF2122/97 SYKRLVQGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSAC
Rv2222c|M.tuberculosis_H37Rv SYKRLVQGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSAC
MMAR_3294|M.marinum_M SYKRLILGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRIEVRSPDSAC
MUL_1335|M.ulcerans_Agy99 SYKRLILGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRIEVRSPDSAC
MAV_2244|M.avium_104 SYKRLVGGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSAC
MLBr_01631|M.leprae_Br4923 SYKRLVVGGEAPTTASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSAC
TH_3396|M.thermoresistible__bu SYKRLVHGGEAPTAASWGAANRSALVRVPMYTPHKASSRRVEVRSPDSAC
MAB_1920|M.abscessus_ATCC_1997 SYKRLVQGGEAPTAASWGAANRSALVRVPMYTPNKSSSRRIEVRSPDSAC
Mflv_1300|M.gilvum_PYR-GCK SYKRTG-AVATLSGASWAPRLPTYGGNDRTHYIRVPDGDRIEMRGGDGSA
Mvan_5509|M.vanbaalenii_PYR-1 SYKRTG-AVATSSGASWAPRLPTYGGNDRTHYIRVPDSDRIEMRGGDGSA
MSMEG_6260|M.smegmatis_MC2_155 SYKRTG-ATSTASGASWAPRLPSYGGNDRTHYIRVPDNQRIEMRGGDGSA
**** . : : ***.. : . : . ... *:*:*. *.:.
Mb2246c|M.bovis_AF2122/97 NPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSS
Rv2222c|M.tuberculosis_H37Rv NPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSS
MMAR_3294|M.marinum_M NPYLAFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRTMGYRELPTS
MUL_1335|M.ulcerans_Agy99 NPYLAFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRTMGYRELPTS
MAV_2244|M.avium_104 NPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTS
MLBr_01631|M.leprae_Br4923 NPYLAFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERLAMGYRELPTS
TH_3396|M.thermoresistible__bu NPYLTFAVLLAAGLRGVEKGYVLGPEAEDNVWTLTPEERRAMGFKELPSS
MAB_1920|M.abscessus_ATCC_1997 NPYLTYAVLLAAGLRGVEQGYTLGPEAEDDVWGLTRAERQAMGYKELPST
Mflv_1300|M.gilvum_PYR-GCK NPYLAIAAALGAGLDGIKRSTDPG------------AVGQGVSTQPLPAT
Mvan_5509|M.vanbaalenii_PYR-1 NPYLAIAAALGAGLDGIKRSTDPG------------AVGQGVSTRALPLT
MSMEG_6260|M.smegmatis_MC2_155 NPYLAVAAALGAGLDGIKRSIDPG------------PVGSGG-TTALPPT
****: *. *.*** *:::. * ** :
Mb2246c|M.bovis_AF2122/97 LDSALRAMEASELVA---EALGEHVFDFFLRNKRTEWANYRSHVTPYELR
Rv2222c|M.tuberculosis_H37Rv LDSALRAMEASELVA---EALGEHVFDFFLRNKRTEWANYRSHVTPYELR
MMAR_3294|M.marinum_M LDSALRAMESSELVA---EALGEHVFDFFLRNKRREWATYRSHVTPYELS
MUL_1335|M.ulcerans_Agy99 LDSALRAMESSELVA---EALGEHVFDFFLRNKRREWATYRSHVTPYELS
MAV_2244|M.avium_104 LDSALRAMESSELVA---ETLGEHVFDFFLRNKRTEWATYRSHVTPYELR
MLBr_01631|M.leprae_Br4923 LDSALGAMEVSEFVA---EALGEHVFDFFLRNKRAEWANYRSHVSLYELK
TH_3396|M.thermoresistible__bu LGQALAEMERSELVA---EALGEHVFDFFLRNKRAEWENYRSHVTPYELK
MAB_1920|M.abscessus_ATCC_1997 LENALIAMEGSELVA---EALGEHVFDFFLRNKRAEWARYRSNVTPFELR
Mflv_1300|M.gilvum_PYR-GCK LLHAVEAFETNPVVTGVLDAAGEGVASYFAAIKRDEFFAYHSSVSPWEID
Mvan_5509|M.vanbaalenii_PYR-1 LLHAVEDFEGDAVVTGALNAAGEGVAAYFANIKREEFFAYHSTVGAWELD
MSMEG_6260|M.smegmatis_MC2_155 LLHAVDALEADPVVTGVLDAAGEGVATYFANLKREEFFTYHGTVTPWEVD
* *: :* . .*: :: ** * :* ** *: *:. * :*:
Mb2246c|M.bovis_AF2122/97 TYLSL-
Rv2222c|M.tuberculosis_H37Rv TYLSL-
MMAR_3294|M.marinum_M TYLSL-
MUL_1335|M.ulcerans_Agy99 TYLSL-
MAV_2244|M.avium_104 TYLSL-
MLBr_01631|M.leprae_Br4923 TYLSL-
TH_3396|M.thermoresistible__bu NYLSL-
MAB_1920|M.abscessus_ATCC_1997 AYLGL-
Mflv_1300|M.gilvum_PYR-GCK YYLTAF
Mvan_5509|M.vanbaalenii_PYR-1 NYLTAF
MSMEG_6260|M.smegmatis_MC2_155 TYLTAF
**