For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. smegmatis MC2 155 MSMEG_2522 (-)

VETAESVSPSPTSTPPRSPQIATTTGNTFGCLVRFAFANIRRRPERFVLSVLGIALAIACVTVVRTISSS
FAITGADSVTDVLGDAQLWVVPAAGVHYDSEASALVADGPAPALTVPDGWRAVQTSSGVVHIGDAEVSLR
GDDEIPAGQAVLGPDVANRLAVADGSVVDVNGHPLTARITGSGESVTVPTSVARSLVGDHGWWTVYAPAG
EENRRDLAQRFGAAVGLPYTADPSEQPKADGEGLIYDTVGGSGPLTFEQKFSALFSGKVTSSTLGLISTI
GLILGFVIAVSSFLAAVQERKREFGIMSSIGLADEVLYFFLVESAIVFVAAYVLGVLGAGAAVALTIPGI
ATPVAWAQAAGMVAAFLPAMAIVGALVPVHRLLQQRPVDLLGAR

Operon Prediction Model: Genebank

Paralogs

species	id	gene	e-value	identity (len)	annotation
M. smegmatis MC2 155	MSMEG_2522	-	-	100% (394)	efflux ABC transporter, permease protein

Closest Orthologs (e-value cutoff: 1e-4)

species	id	gene	e-value	identity (len)	annotation
M. bovis AF2122 / 97	-	-	-	-	-
M. gilvum PYR-GCK	Mflv_4129	-	1e-149	70.08% (381)	hypothetical protein Mflv_4129
M. tuberculosis H37Rv	-	-	-	-	-
M. leprae Br4923	-	-	-	-	-
M. abscessus ATCC 19977	MAB_4672c	-	3e-10	23.48% (379)	ABC transporter permease
M. marinum M	-	-	-	-	-
M. avium 104	-	-	-	-	-
M. thermoresistible (build 8)	-	-	-	-	-
M. ulcerans Agy99	-	-	-	-	-
M. vanbaalenii PYR-1	Mvan_2214	-	1e-154	71.36% (391)	hypothetical protein Mvan_2214

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_4129|M.gilvum_PYR-GCK          -----------MPTAIRAPQPAAGSGNTVTCLIRFALANIRRRPERFVLA
Mvan_2214|M.vanbaalenii_PYR-1       ---MEATQVGSAPAAVRSPQIPAGSGNTFTCLVRFALANIRRRPERFVLA
MSMEG_2522|M.smegmatis_MC2_155      METAESVSPSPTSTPPRSPQIATTTGNTFGCLVRFAFANIRRRPERFVLS
MAB_4672c|M.abscessus_ATCC_199      --------------------------------MIVAVKNLVAERARFVLS
                                                                    : .*. *:  .  ****:

Mflv_4129|M.gilvum_PYR-GCK          VLGIALAIACVTVVRTISASFAATGEESVADVLGSAALWVVPAAGVHYDA
Mvan_2214|M.vanbaalenii_PYR-1       VLGIALAIACVTIVRTISAGFATTGEASVADVLADGQLWVVPAAGVHYDP
MSMEG_2522|M.smegmatis_MC2_155      VLGIALAIACVTVVRTISSSFAITGADSVTDVLGDAQLWVVPAAGVHYDS
MAB_4672c|M.abscessus_ATCC_199      VVGVAVAVILVLVMSGIFVGTTNQVTTYIDHSKG--AVWVVQPGVSQMFR
                                    *:*:*:*:  * ::  *  . :      : .  .   :*** ..  :   

Mflv_4129|M.gilvum_PYR-GCK          QAGALIADGDAPEISIPGGWTATRVLSGLVDIDGEGVSLRGSDEIASGTA
Mvan_2214|M.vanbaalenii_PYR-1       AAEALIADGPAPALAAPEGWTATHVVSGLVEVNGQAVALRGTGQATAGQA
MSMEG_2522|M.smegmatis_MC2_155      EASALVADGPAPALTVPDGWRAVQTSSGVVHIGDAEVSLRGDDEIPAGQA
MAB_4672c|M.abscessus_ATCC_199      AVSWLPAEGKDRLLKVPG----VQSAEPILGLPSDFVHKGGHTAYFVVGY
                                     .  * *:*    :  *     .:  . :: : .  *   *         

Mflv_4129|M.gilvum_PYR-GCK          TVGHALAERLAVVDGGTVTVGGRRLQVSVEAAGQSLTVQTALARSLVGER
Mvan_2214|M.vanbaalenii_PYR-1       LVGSAAAERLSVSDGDRVTIGGQQLAVRVSGPGQSVTVPTPVAQATVGDN
MSMEG_2522|M.smegmatis_MC2_155      VLGPDVANRLAVADGSVVDVNGHPLTARITGSGESVTVPTSVARSLVGDH
MAB_4672c|M.abscessus_ATCC_199      DTATGIGGPWSLAEGRNVAGSGEVVLDRVLAKKNKIHLGDTVRLVDGDFT
                                      .   .   :: :*  *  .*. :   : .  :.: :  .:     .  

Mflv_4129|M.gilvum_PYR-GCK          GWWLVNAPPGGEHRRDLGAAFGAAVGLPFTPDPA---VRPDGDGAG-LVY
Mvan_2214|M.vanbaalenii_PYR-1       GWWVVRAPAGEQQRRDLAQVFGAAVGLPWTADPA---VQPDAGGAG-LIY
MSMEG_2522|M.smegmatis_MC2_155      GWWTVYAPAGEENRRDLAQRFGAAVGLPYTADPS---EQPKADGEG-LIY
MAB_4672c|M.abscessus_ATCC_199      VVGLSKQTAAVGNFYAFISLVDAAHLLRAGNRVSYFLVQPTVGFTGDQVV
                                           ...  :   :   ..**  *      :    :*  .  *  : 

Mflv_4129|M.gilvum_PYR-GCK          DTVGGSGP--LTFEQKYSALFSGKVTSSTLG----LISTIGLGLGFVIAV
Mvan_2214|M.vanbaalenii_PYR-1       DTVGGSGP--LTFEQKYSALFSGQVTSSTLG----LISTIGLGLGFVIAV
MSMEG_2522|M.smegmatis_MC2_155      DTVGGSGP--LTFEQKFSALFSGKVTSSTLG----LISTIGLILGFVIAV
MAB_4672c|M.abscessus_ATCC_199      DSVRKSVPGMDAFTSETFAENSRAIVISMIGRPLRTMIGIAALVGVALVG
                                    *:*  * *   :* .:  *  *  :. * :*     :  *.  :*..:. 

Mflv_4129|M.gilvum_PYR-GCK          SSFLAAVTERRREFGIMSSIGLADEVLY-FFLVESAVIFVTAYLVGVTAA
Mvan_2214|M.vanbaalenii_PYR-1       SSFLAAVTERRREFGIMSSIGLADEVLY-FFLVESAIVFLAAYVVGIAAA
MSMEG_2522|M.smegmatis_MC2_155      SSFLAAVQERKREFGIMSSIGLADEVLY-FFLVESAIVFVAAYVLGVLGA
MAB_4672c|M.abscessus_ATCC_199      LTVLAVTTEQMSDFGVLRALGVRPVQLCRTVIAQAALIAGLGYILGAGIT
                                     :.**.. *:  :**:: ::*:    *   .:.::*::   .*::*   :

Mflv_4129|M.gilvum_PYR-GCK          GIAVAVVIPGIASVASWLQGAALTAMFLP--AMAIVGALVPVHRLLQQRP
Mvan_2214|M.vanbaalenii_PYR-1       GIAVALVIPGIASVGSWLQGAALTAMFLP--AMAIVGALVPVHRLLQQRP
MSMEG_2522|M.smegmatis_MC2_155      GAAVALTIPGIATPVAWAQAAGMVAAFLP--AMAIVGALVPVHRLLQQRP
MAB_4672c|M.abscessus_ATCC_199      YGVQFLVKDRLGDVTVEVTPAMLAGMAAATGVMAVIGSLIPVRRVTRIDP
                                      .  :.   :.        * :..   .  .**::*:*:**:*: :  *

Mflv_4129|M.gilvum_PYR-GCK          VSLLGDR
Mvan_2214|M.vanbaalenii_PYR-1       VSLLGDR
MSMEG_2522|M.smegmatis_MC2_155      VDLLGAR
MAB_4672c|M.abscessus_ATCC_199      AQAFRR-
                                    .. :