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MASVLWFQGGACSGNTMSFLNADEPNVVDLIVDFGLDLLWHPSLGLELGNNAQKVFWDCAKGERPLDIFV FEGTVIEAPNGTGQMDMFAGRPMKDWVTDLAGAAQIVVAIGDCACFGGIPAMEPNPSGSTGLQFHKREKG GFLGPDFRSKMGLPVINVPGCPAHPDWITQILVALATGRAGDITLDDLHRPETFFKTFTQTGCTRVQFFE YKQSTLSFGEGTRTGCLFYEFGCRGPMTHSPCNRILWNRQSSKTRAGMPCLGCTEPEFPHFDLAPGTVFK TQKVSGMIPKEVPEGTDHLTYMGLAAAARIAAPQWSKEDMFVV
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2262 | hybA | - | 100% (323) | hydrogenase-2, small subunit |
| M. smegmatis MC2 155 | MSMEG_2720 | - | 5e-28 | 31.40% (293) | NADH ubiquinone oxidoreductase, 20 kDa subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. gilvum PYR-GCK | - | - | - | - | - |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_1870 | hyaA | 1e-29 | 32.42% (293) | nickel/iron-hydrogenase I small subunit, HyaA |
| M. avium 104 | MAV_2681 | - | 1e-26 | 30.10% (289) | NADH ubiquinone oxidoreductase, 20 kDa subunit |
| M. thermoresistible (build 8) | TH_1031 | hybA | 1e-176 | 92.83% (307) | hydrogenase-2, small subunit |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_2365 | - | 0.0 | 95.05% (323) | NADH ubiquinone oxidoreductase, 20 kDa subunit |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_2262|M.smegmatis_MC2_155 ------------MASVLWFQG-GACSGNTMSFLNADEPNVVDLIVDFGLD
TH_1031|M.thermoresistible__bu -----------------------------MSFLNADEPNVVDLIVDFGLD
Mvan_2365|M.vanbaalenii_PYR-1 ------------MASVLWFQG-GACSGNTMSFLNAEEPNVVDLIVDFGLD
MMAR_1870|M.marinum_M MPTEAAVKAEETLIHVLWINAGLSCDGDSVALTAATQPSVEEIALGALPG
MAV_2681|M.avium_104 MPTQAAVKAEETLIHVLWINAGLSCDGDSVALTAATQPTIEEIALGALPG
::: * :*.: :: :. .
MSMEG_2262|M.smegmatis_MC2_155 L----LWHPSLGLELG-----NNAQKVFWDCAKGERPLDIFVFEGTVIE-
TH_1031|M.thermoresistible__bu I----LWHPSLGMELG-----DNAQKVFWDCVNGERPLDIFVFEGTVIR-
Mvan_2365|M.vanbaalenii_PYR-1 L----LWHPSLGLELG-----ENAQKVFWDCANGQRPLDIFVFEGTVMQ-
MMAR_1870|M.marinum_M LPQVAVHWPLIDFECGPSGGADDFLAWFFKAERGELEPFVLVVEGSIPNE
MAV_2681|M.avium_104 LPKIAVHWPLIDFECGPEGGADDFLSWFFKADRGELEPFVLVVEGSIPNE
: : * :.:* * :: *:.. .*: ::*.**:: .
MSMEG_2262|M.smegmatis_MC2_155 APNGTGQMDMFAGRP-------MKDWVTDLAGAAQIVVAIGDCACFGGIP
TH_1031|M.thermoresistible__bu APNGSGRMDMFAGRP-------MMDWVTDLAGAAQIVVGIGDCASFGGIP
Mvan_2365|M.vanbaalenii_PYR-1 AYG--GRTDMFADRP-------MKDWVTDLAGAAQIVVAIGDCACWGGIP
MMAR_1870|M.marinum_M KIKNEGYWCGFGNDPATGQPMTTSEWLDRLTPKATAVVAVGTCATYGGIH
MAV_2681|M.avium_104 NLHDEGYWCGFGNNPGTGQPVTTSEWLDRLAPKATAIVCAGTCSCYGGIH
* *.. * :*: *: * :* * *: :***
MSMEG_2262|M.smegmatis_MC2_155 AMEPNPSGSTGLQFHKREKGGFLGPDFRSKMGLPVINVPGCPAHPDWITQ
TH_1031|M.thermoresistible__bu AMAPNPSDSTGLQFHKREKGGFLGADFRSKMGLPVINIPGCPAHPDWITQ
Mvan_2365|M.vanbaalenii_PYR-1 AMEPNPSGSTGLQFHKREKGGFLGPDFRSKMGLPVINVPGCPAHPDWITQ
MMAR_1870|M.marinum_M AMAGNPTGAMGVP-------DYLGWDWKSKAGIPIVCVPGCPIHPDNLSE
MAV_2681|M.avium_104 AMAGNPTGAMGVP-------DYLGWDWKSKAGIPIVCVPGCPTQGDNLSE
** **:.: *: .:** *::** *:*:: :**** : * :::
MSMEG_2262|M.smegmatis_MC2_155 ---ILVALATGRAGDITLDDLHRPETFFKTFTQTGCTRVQFFEYKQSTLS
TH_1031|M.thermoresistible__bu ---ILVALASGRAGDITLDELHRPETFFKTFTQTGCTRVQFFEYKQSTMS
Mvan_2365|M.vanbaalenii_PYR-1 ---ILVALATGRAGDITLDDLHRPETFFKTFTQTGCTRVQFFEYKQSTLS
MMAR_1870|M.marinum_M TLTYLLYMATGQAPMIPLDDALRPKWLFGNTVHEGCDRAGYYEQGDFATE
MAV_2681|M.avium_104 TLTYLLYMATGQAPMIPLDDELRPTWLFGNTVHEGCDRAGYYEQGDFATE
*: :*:*:* *.**: ** :* . .: ** *. ::* : : .
MSMEG_2262|M.smegmatis_MC2_155 FGEGTRTGCLFYEFGCRGPMTHSPCNRILWNR-QSSKTRAGMPCLGCTEP
TH_1031|M.thermoresistible__bu FGEGTRTGCLFYEFGCRGPMTHSPCNRILWNR-QSSKTRAGMPCLGCTEP
Mvan_2365|M.vanbaalenii_PYR-1 FGEGTRTGCLFYEFGCRGPMTHSPCNRILWNR-QSSKTRAGMPCIGCTEP
MMAR_1870|M.marinum_M YGSPK----CIVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIGCTMP
MAV_2681|M.avium_104 YGSPK----CIVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIGCTMP
:*. . : ::** **:.:. : * . .. ...* *:*** *
MSMEG_2262|M.smegmatis_MC2_155 EFPHFDLAPGTVFKTQKVSGMIPKEVP--EGTDHLTYMGLAAAARIAAPQ
TH_1031|M.thermoresistible__bu EFPHFDLAPGTLFKTQKVSGTIPKEVP--EGTDHLTYMAHAAAARIAAPQ
Mvan_2365|M.vanbaalenii_PYR-1 EFPHFDLAPGTLFKTQKVSGMIPKEVP--EGTDHLTYMGLAAAARIAAPQ
MMAR_1870|M.marinum_M GFPDKFMP----FMDEPPGGKLSSTASGLYGTVIRSLRNITERTVDKEPR
MAV_2681|M.avium_104 GFPDKFMP----FMDEPPGGLISSTASGLYGSFIRPLRRITGRTLNKEPR
**. :. * : .* :.. .. *: . : : *:
MSMEG_2262|M.smegmatis_MC2_155 WSKEDMFVV-------
TH_1031|M.thermoresistible__bu WSKEDMFVV-------
Mvan_2365|M.vanbaalenii_PYR-1 WSKEDMFVV-------
MMAR_1870|M.marinum_M WRHKGTQLTTGARRTW
MAV_2681|M.avium_104 WRHKGKQLTTGARRTW
* ::. :.