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M. smegmatis MC2 155 MSMEG_0377 (-)

annotation: nitrile hydratase
coordinates: 422996 - 423736
length: 246

MRLQHFIGGLEGLTGPLDFEKRVFVEDWEKRIFGIHVAMMALSTHLGEALPGYRVADVPTTFREKWTWAH
LRTGAEGMNPFDYFKFRYYEKWLGGISQFFVDKGYLSEDEIRAAPVGSPPKAPAPGVKAAIDDQVLDYLR
RGDSPRRGSAIPRFAPGDKVRIADVPAGEHTRLPGYLRNRTGTVERCFEGEYSYFVHTGDGIGDPMPIYI
VEFTPRELWGARAEPGASALYAELFEAYLSPVEEDQ
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_0377--100% (246)nitrile hydratase
M. smegmatis MC2 155MSMEG_6266-2e-0522.94% (170) thiocyanate hydrolase beta subunit

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97-----
M. gilvum PYR-GCKMflv_1010-1e-11074.80% (246) nitrile hydratase
M. tuberculosis H37Rv-----
M. leprae Br4923-----
M. abscessus ATCC 19977-----
M. marinum M-----
M. avium 104-----
M. thermoresistible (build 8)-----
M. ulcerans Agy99-----
M. vanbaalenii PYR-1Mvan_5826-1e-11177.73% (247) nitrile hydratase

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_1010|M.gilvum_PYR-GCK          MKLQHYLGGLEGLPEPLNLEKKVFVEDWEKRIFGIHVAMMGLSNHLGSAL
Mvan_5826|M.vanbaalenii_PYR-1       MKLQHNLGGLEGLPQPLTLEKRVFVEEWEKRIFGIHVAMMGLSNHLGSAL
MSMEG_0377|M.smegmatis_MC2_155      MRLQHFIGGLEGLTGPLDFEKRVFVEDWEKRIFGIHVAMMALSTHLGEAL
                                    *:*** :******. ** :**:****:*************.**.***.**

Mflv_1010|M.gilvum_PYR-GCK          PGYPIAEVPTTFRDEWTWASLRTGAEGMNPFDYFKFRYYEKWLGGISQFF
Mvan_5826|M.vanbaalenii_PYR-1       PEYPIAEVPTAFRDEWTWADLRTGAEAMNPFDYFKFRYYEKWLGGITQFF
MSMEG_0377|M.smegmatis_MC2_155      PGYRVADVPTTFREKWTWAHLRTGAEGMNPFDYFKFRYYEKWLGGISQFF
                                    * * :*:***:**::**** ******.*******************:***

Mflv_1010|M.gilvum_PYR-GCK          VDNGYVSEEEIADLLGVTDPPVAGPGS-PAIDEQVIDYLRRGDSPRRDVA
Mvan_5826|M.vanbaalenii_PYR-1       IDKGYVSEDELAAAVGAAAPSPDGAG--DAIDDQVIDYLRRGDSPRRDVA
MSMEG_0377|M.smegmatis_MC2_155      VDKGYLSEDEIRAAPVGSPPKAPAPGVKAAIDDQVLDYLRRGDSPRRGSA
                                    :*:**:**:*:      : *   ..*   ***:**:***********. *

Mflv_1010|M.gilvum_PYR-GCK          HPKFEVGDTVLITNAPAGAHTRLPGYLRAKTGTVQRIFEGDYGYFVHTGD
Mvan_5826|M.vanbaalenii_PYR-1       HPRFAVGQKVRITDVPAGAHTRLPGYLRARTGTVQRIFEGDYGYFVETGD
MSMEG_0377|M.smegmatis_MC2_155      IPRFAPGDKVRIADVPAGEHTRLPGYLRNRTGTVERCFEGEYSYFVHTGD
                                     *:*  *:.* *::.*** ********* :****:* ***:*.***.***

Mflv_1010|M.gilvum_PYR-GCK          GIGDPMPIYIVEFTPEELWGPRAEPGANALYAELFEAYLEPIEEDK
Mvan_5826|M.vanbaalenii_PYR-1       GIGDPMPIYIVEFTPHELWGVRAEPGANTLYAELFEAYLQPVEENQ
MSMEG_0377|M.smegmatis_MC2_155      GIGDPMPIYIVEFTPRELWGARAEPGASALYAELFEAYLSPVEEDQ
                                    ***************.**** ******.:**********.*:**::