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RPLTIIGPTGTGKSDLAIEIADRLSGKIAVEIVNADAYQLYRGMDIGTGKVPLAQRRGIPHHQLDVLDVT ETATVAGYQRSAAADIEAIASRGALPLLVGGSMLYVQSLLDDWAFPAKDPAIRARWERRLAQVGPARLHA ELVRRDPAAAAVIPLNDARRTVRALEVVEITGRPYAASAPRIGSPRWDSAIIGLDCETKVLDERLAARTK AMFDRGLIEEVISLLPCGLARGVTASRALGYAQVMEALKAGADTQALDSARQQTCLATRRYVRRQRSWFR RDRRVRWLDATVSTAAHRTAIIEAVLGAWRRAS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_1561 | miaA_1 | - | 100% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase MiaA_1 |
| M. marinum M | MMAR_1986 | miaA | e-119 | 66.24% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase MiaA |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2746c | miaA | 1e-128 | 70.70% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. gilvum PYR-GCK | Mflv_3962 | miaA | 1e-114 | 66.35% (312) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. tuberculosis H37Rv | Rv2727c | miaA | 1e-128 | 70.70% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. leprae Br4923 | MLBr_00995 | miaA | 1e-122 | 68.15% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. abscessus ATCC 19977 | MAB_3044c | - | 1e-106 | 61.66% (313) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. avium 104 | MAV_3620 | miaA | 1e-122 | 70.42% (311) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. smegmatis MC2 155 | MSMEG_2734 | miaA | 1e-108 | 64.54% (313) | tRNA delta(2)-isopentenylpyrophosphate transferase |
| M. thermoresistible (build 8) | TH_1518 | miaA | 1e-107 | 60.94% (320) | PROBABLE TRNA DELTA(2)-ISOPENTENYLPYROPHOSPHATE TRANSFERASE |
| M. ulcerans Agy99 | MUL_3469 | miaA_1 | 1e-174 | 98.73% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase MiaA_1 |
| M. vanbaalenii PYR-1 | Mvan_2434 | miaA | 1e-113 | 65.29% (314) | tRNA delta(2)-isopentenylpyrophosphate transferase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_3962|M.gilvum_PYR-GCK --MRPLAVVGPTGTGKSDLALGIAEALSGEIAVEVVNADAMQLYRGMDIG
Mvan_2434|M.vanbaalenii_PYR-1 --MRPLAIVGPTGTGKSDLALAVAGLLSAEIAVEVVNADAMQLYRGMDIG
MSMEG_2734|M.smegmatis_MC2_155 -MNRPLAVIGPTGTGKSALALEFAERVGG----EIVNADAMQQYRGMDIG
TH_1518|M.thermoresistible__bu MTDRPLAIVGPTGTGKSDLAVAVAERIGG----EIVNADAMQQYRGMDIG
MAB_3044c|M.abscessus_ATCC_199 -MTRPIAVIGPTATGKSALALELAERLGG----EIVNADAMQLYRGMDIG
Mb2746c|M.bovis_AF2122/97 --MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIG
Rv2727c|M.tuberculosis_H37Rv --MRPLAIIGPTGAGKSQLALDVAARLGARVSVEIVNADAMQLYRGMDIG
MLBr_00995|M.leprae_Br4923 --MRPLAIVGPTGVGKSELALDVIERLGGQVSVEIVNADAMQLYRGMDIG
MAV_3620|M.avium_104 -----MAIIGPTGTGKSQLALDVAERLG-PLGAEIVNADAMQLYRGMDIG
MMAR_1561|M.marinum_M --MRPLTIIGPTGTGKSDLAIEIADRLSGKIAVEIVNADAYQLYRGMDIG
MUL_3469|M.ulcerans_Agy99 --MRPLTIIGPTGTGKSDSAIEIADRLSSKIAVEIVNADAYQLYRGMDIG
::::***..*** *: . :. *:***** * *******
Mflv_3962|M.gilvum_PYR-GCK TAKLTVDERRGVPHHQLDVLEVTETATVARYQQAAAADVDAIAARGALPV
Mvan_2434|M.vanbaalenii_PYR-1 TAKLTVVERHGVPHHQLDVLDVTETATVARYQQAAAADVEAIAARGALPV
MSMEG_2734|M.smegmatis_MC2_155 TAKLTVEERRGIPHHQLDVLDVVETATVARYQQAAAADIETIAARGATPV
TH_1518|M.thermoresistible__bu TAKLTVAERRGIPHHQLDVLEVTETATVARYQQAAAADVEAVIARGRVPV
MAB_3044c|M.abscessus_ATCC_199 TAKVPECERRGLVHHMLDVLDVTETATVATYQERAVATIDDIRARGHVPV
Mb2746c|M.bovis_AF2122/97 TAKLPVSERRGIPHHQLDVLDVTETATVARYQRAAAADIEAIAARGAVPV
Rv2727c|M.tuberculosis_H37Rv TAKLPVSERRGIPHHQLDVLDVTETATVARYQRAAAADIEAIAARGAVPV
MLBr_00995|M.leprae_Br4923 TAKLPVAARRGIPHHQLDVLDVTETATVASYQRTAAADIEAIAARGAVPV
MAV_3620|M.avium_104 TAKLPVDARRGIPHHQLDVLDVTQTATVARYQRAAAADIEAILAAGAVPI
MMAR_1561|M.marinum_M TGKVPLAQRRGIPHHQLDVLDVTETATVAGYQRSAAADIEAIASRGALPL
MUL_3469|M.ulcerans_Agy99 TGKVPLAQRRGIPHHQLDVLDVTETATVAGYQRSAAADIEAIASRGALPL
*.*:. *:*: ** ****:*.:***** **. *.* :: : : * *:
Mflv_3962|M.gilvum_PYR-GCK IVGGSMLYVQSLLDEWSFPATDPQVRARWETRLAEVGVAALHRELAAVDA
Mvan_2434|M.vanbaalenii_PYR-1 IVGGSMLYVQSLLDQWSFPATDPRVRTRWERRLAEIGVAALHHELADVDA
MSMEG_2734|M.smegmatis_MC2_155 IVGGSMLYIQSLLDEWSFPATDPAVRAKYEARLAEIGVAALHAELARVDA
TH_1518|M.thermoresistible__bu IVGGSMMYIQALLDDWSIPATDPAVRARWEARLAEIGARALHAELARVDS
MAB_3044c|M.abscessus_ATCC_199 IVGGSMMYIQALLDDWAFPATDPQVRARWEERLAQIGVTALHAELAARDP
Mb2746c|M.bovis_AF2122/97 VVGGSMLYVQSLLDDWSFPATDPSVRARWERRLAEVGVDRLHAELARRDP
Rv2727c|M.tuberculosis_H37Rv VVGGSMLYVQSLLDDWSFPATDPSVRARWERRLAEVGVDRLHAELARRDP
MLBr_00995|M.leprae_Br4923 VVGGSMLYVQSLLDDWSFPGTDPAVRVRWEQQLAEVGVVRLHAELARRDL
MAV_3620|M.avium_104 IVGGSMLYIQSLLDDWSFPATDPRVRARWERRLAEVGVGELHAELARRDP
MMAR_1561|M.marinum_M LVGGSMLYVQSLLDDWAFPAKDPAIRARWERRLAQVGPARLHAELVRRDP
MUL_3469|M.ulcerans_Agy99 LVGGSMLYVQSLLDDWAFPAKDPAIRARWERRLAQVGPARLHADLVRRDP
:*****:*:*:***:*::*..** :*.::* :**::* ** :*. *
Mflv_3962|M.gilvum_PYR-GCK AAAASILPTDGRRIVRALEVVELTGRPFAASAPTIGAPRWNTAIVGLDWE
Mvan_2434|M.vanbaalenii_PYR-1 AAAASILPTDGRRIVRALEVVELTGQPFAASAPTIGAPRWGTVIVGLDWD
MSMEG_2734|M.smegmatis_MC2_155 AAAASILPTDGRRIVRALEVVELTGEPFAASAPTIGAPRWDTAIIGLDWD
TH_1518|M.thermoresistible__bu AAADTILPTDGRRIVRALEVIELTGRPFAASKPPVGPPRWDTVIIGLDWA
MAB_3044c|M.abscessus_ATCC_199 AAAAIILPTDGRRTVRALEVIELTGQPFAASAPTIGPPRWDTLIVGLDWE
Mb2746c|M.bovis_AF2122/97 AAAAAILPTDARRTVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCQ
Rv2727c|M.tuberculosis_H37Rv AAAAAILPTDARRTVRALEVVELTGQPFAASAPRIGAPRWDTVIVGLDCQ
MLBr_00995|M.leprae_Br4923 AAAAAILPTDGRRTVRALEVVELTGKPFAASAPRIGAPRWDTVIVGLDCD
MAV_3620|M.avium_104 AAAAAILPTDARRTVRALEVIELTGRPFAASAPRIGAPRWDTVIIGLDCD
MMAR_1561|M.marinum_M AAAAVIPLNDARRTVRALEVVEITGRPYAASAPRIGSPRWDSAIIGLDCE
MUL_3469|M.ulcerans_Agy99 AAAAVIPLNDARRTVRALEVVEITGRPYAASAPRIGSPRWDSAIIGLDCE
*** * .*.** ******:*:**.*:*** * :*.***.: *:***
Mflv_3962|M.gilvum_PYR-GCK TTVLDERLRRRTDTMFERGLVEEVRGLIGRGLRDGVTAARALGYAQVLAD
Mvan_2434|M.vanbaalenii_PYR-1 TTVLDERLARRTDLMFERGLVDEVVGLLGVGLREGVTAARALGYAQVLAD
MSMEG_2734|M.smegmatis_MC2_155 TAVLDERLAQRTDKMFADGLVREVVDLLERGLRDGVTAARALGYAQVLAD
TH_1518|M.thermoresistible__bu TEALDERLRQRTDKMFAAGLVDEVRGLIERGLRKGVTASRALGYAQVLAA
MAB_3044c|M.abscessus_ATCC_199 TEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYAQVIAA
Mb2746c|M.bovis_AF2122/97 TTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRALGYAQVIAA
Rv2727c|M.tuberculosis_H37Rv TTILDERLARRTDLMFDQGLVEEVRTLLRNGLREGVTASRALGYAQVIAA
MLBr_00995|M.leprae_Br4923 RTILSERLARRIDSMFGQGLVDEVRMLLRWGLRDGVTASRALGYAQVLTA
MAV_3620|M.avium_104 TTVLDERLARRTDAMFEQGLVAEVTGLLGRGLRDGVTAARALGYAQVIAA
MMAR_1561|M.marinum_M TKVLDERLAARTKAMFDRGLIEEVISLLPCGLARGVTASRALGYAQVMEA
MUL_3469|M.ulcerans_Agy99 TKVLDERLAARTKAMFDRGLIEEVISLLPCGLARGVTASRALGYAQVMEA
*.:*: * . ** *:: ** *: ** ****:**:*****:
Mflv_3962|M.gilvum_PYR-GCK LDAGGDGEA----------AREPTFVGTRRYVRRQRSWFRRDHRITWLDG
Mvan_2434|M.vanbaalenii_PYR-1 LDAGGDGSA----------AREPTFVGTRRYVRRQRSWFRRDHRITWLDG
MSMEG_2734|M.smegmatis_MC2_155 LDAGGDGSG----------AREPTFIGTRRYVRRQRSWFRRDHRVVWLDG
TH_1518|M.thermoresistible__bu LDTGTGPAAQSAAGEALAEARERTFIGTRRYVRRQRSWFRRDHRIIWLDG
MAB_3044c|M.abscessus_ATCC_199 LDAGGGAGA-------LAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAG
Mb2746c|M.bovis_AF2122/97 LDAGAGADM-------MRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDA
Rv2727c|M.tuberculosis_H37Rv LDAGAGADM-------MRAAREQTYLGTRRYVRRQRSWFRRDHRVHWLDA
MLBr_00995|M.leprae_Br4923 LDAGGDADH-------LDEARQQTYLGHRRYARRQRSWFHRDHRVHWLDV
MAV_3620|M.avium_104 LDAGGGDEQ-------LRQAREQTYAGTRRYVRRQRSWFRRDHRVRWLDA
MMAR_1561|M.marinum_M LKAGADTQA-------LDSARQQTCLATRRYVRRQRSWFRRDRRVRWLDA
MUL_3469|M.ulcerans_Agy99 LKAGADTQA-------LDRARQQTCLATRRYVRRQRSWFRRDRRVRWLDA
*.:* . **: * . ***.******* **:*: **
Mflv_3962|M.gilvum_PYR-GCK AAPGN------VDAVLRLWRQRRAGAPRPAD
Mvan_2434|M.vanbaalenii_PYR-1 AAGGSAA---LADKVIRIWRDVS--------
MSMEG_2734|M.smegmatis_MC2_155 ASEGL------VDDALRVWRAVS--------
TH_1518|M.thermoresistible__bu AAPDN------VERALATWRNVS--------
MAB_3044c|M.abscessus_ATCC_199 ESVETGGPEGLAGEIVSRWRLSSNT------
Mb2746c|M.bovis_AF2122/97 GVASSPDRARLVDDAVRLWRHVT--------
Rv2727c|M.tuberculosis_H37Rv GVASSPDRARLVDDAVRLWRHVT--------
MLBr_00995|M.leprae_Br4923 GTV---DRVGVVDDALRVWRNAS--------
MAV_3620|M.avium_104 GACS---PPRLVDAALGAWRHVS--------
MMAR_1561|M.marinum_M TVSTAAHRTAIIEAVLGAWRRAS--------
MUL_3469|M.ulcerans_Agy99 TVSTAAHRTAIIEAVLGAWRRAS--------
: **