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M. leprae Br4923 MLBr_00322 (ispF)

annotation: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
coordinates: 411601 - 412077
length: 158

VSELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDVAVHALCDAVLSAAGQGDIGGLFAVGDP
RWERVSGADMLRHVVELLLRHGYEVVNASVQVIGNRPKIGPRRTEAQRLLSALLRAPVSVAATTTEGLGL
TGRGEGLSAIATALVVQV

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. leprae Br4923 MLBr_00322 ispF - 100% (158) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3612c ispF 4e-69 79.75% (158) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. gilvum PYR-GCK Mflv_1445 ispF 3e-61 75.50% (151) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. tuberculosis H37Rv Rv3581c ispF 4e-69 79.75% (158) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. abscessus ATCC 19977 MAB_0570 - 8e-61 69.03% (155) putative 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. marinum M MMAR_5081 ispF 7e-70 80.38% (158) 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase IspF
M. avium 104 MAV_0572 ispF 2e-66 76.28% (156) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. smegmatis MC2 155 MSMEG_6075 ispF 1e-56 69.74% (152) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. thermoresistible (build 8) TH_1184 - 5e-63 72.44% (156) PROBABLE 2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE
M. ulcerans Agy99 MUL_4157 ispF 8e-68 80.92% (152) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
M. vanbaalenii PYR-1 Mvan_5339 ispF 3e-61 74.83% (151) 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

CLUSTAL 2.0.9 multiple sequence alignment


MSMEG_6075|M.smegmatis_MC2_155      --------MMLPRVGLGTDVHPIEAGRPCRLLCLEFDDADGCAGHSDGDV
TH_1184|M.thermoresistible__bu      LPPHPDQVMSIPRVGLGTDVHPIEPGRPCWLLGLLFDGVDGCAGHSDGDV
MAB_0570|M.abscessus_ATCC_1997      -------MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDV
Mflv_1445|M.gilvum_PYR-GCK          --------MSTFRVGLGTDVHPIEAGRPCRLLCLLFDDGDGCAGHSDGDV
Mvan_5339|M.vanbaalenii_PYR-1       -------MLPSIRVGLGTDVHPIEAGRPCWLLCLLFDDADGCAGHSDGDV
Mb3612c|M.bovis_AF2122/97           -------MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDV
Rv3581c|M.tuberculosis_H37Rv        -------MNQLPRVGLGTDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDV
MMAR_5081|M.marinum_M               -------MNGLPRVGLGVDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDV
MUL_4157|M.ulcerans_Agy99           -------------MGLGVDVHPIEPGRPCWLVGLLFPSADGCAGHSDGDV
MLBr_00322|M.leprae_Br4923          -------MSELPRVGLGFDVHVFEPGRPCWLVGLLFPDNDGCAGHSDGDV
MAV_0572|M.avium_104                -------MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDV
                                                 :*:* *** :: *:** *: * * . :** *******

MSMEG_6075|M.smegmatis_MC2_155      AAHALCDALLSAAGLGDLGTIFGTDRPQWRGASGADMIRHVRGLVENAGF
TH_1184|M.thermoresistible__bu      AAHALCDALLSAAGLGDLGEVFGTGRPEWQGVTGADMLRHVRGLVEAEGF
MAB_0570|M.abscessus_ATCC_1997      AAHALCDALLSAAGLGDVGAVFGTGRPEWTGVSGAKMLSHVRDLLHDNGF
Mflv_1445|M.gilvum_PYR-GCK          AAHALCDALLSAAGMGDIGEVFGTGLPQWRDVSGADMLRHVHTLLVEQGF
Mvan_5339|M.vanbaalenii_PYR-1       AAHALCDALLSAAGMGDIGEVFGTGLPQWRGVSGADMLRHVHSMMTAEGL
Mb3612c|M.bovis_AF2122/97           AVHALCDAVLSAAGLGDIGEVFGVDDPRWQGVSGADMLRHVVVLITQHGY
Rv3581c|M.tuberculosis_H37Rv        AVHALCDAVLSAAGLGDIGEVFGVDDPRWQGVSGADMLRHVVVLITQHGY
MMAR_5081|M.marinum_M               AAHALCDAVLSAAGLGDIGDVFGVGDPRWEGVSGATMLRHVVELITQHGY
MUL_4157|M.ulcerans_Agy99           AAHALCDAVLSAAGLGDIGDVFGVGDPRWEGVSGATMLRHVVELITQHGY
MLBr_00322|M.leprae_Br4923          AVHALCDAVLSAAGQGDIGGLFAVGDPRWERVSGADMLRHVVELLLRHGY
MAV_0572|M.avium_104                GAHALCDAVLSAAGLGDVGAVFGVDDPRWAGVSGADMLRHVADLTARHGF
                                    ..******:***** **:* :*... *.*  .:** *: **  :    * 

MSMEG_6075|M.smegmatis_MC2_155      VIGNATVQVIGNRPKVGPRREEAQQVLSELVGAPVSVSATTTDGLGLTGR
TH_1184|M.thermoresistible__bu      RIGNAAVQVIGNRPKVGPRRAEAQRLLTELLGAPVSVSATTTDGLGLTGR
MAB_0570|M.abscessus_ATCC_1997      QIGNAAVQVIGNRPKVGPRRAEAQELLSGLIGAPVSVSATTTDGLGLTGR
Mflv_1445|M.gilvum_PYR-GCK          QVGNAAVQVIGNRPKIGPRRAEAQRILSELLDAPVSVSATTTDGLGLTGR
Mvan_5339|M.vanbaalenii_PYR-1       RVGNAAVQVIGNRPKIGPRRAEAQRVLSGILGAPVSVSATTTDGLGLTGR
Mb3612c|M.bovis_AF2122/97           RVGNAVVQVIGNRPKIGWRRLEAQAVLSRLLNAPVSVSATTTDGLGLTGR
Rv3581c|M.tuberculosis_H37Rv        RVGNAVVQVIGNRPKIGWRRLEAQAVLSRLLNAPVSVSATTTDGLGLTGR
MMAR_5081|M.marinum_M               RVGNASVQVVGNRPKIGWRRYEAQAVLSRLLNAPVSVSATTTDGLGLTGR
MUL_4157|M.ulcerans_Agy99           RVGNASVQVVGNRPKIGWRRYEAQAVLSRLLNAPVSVSATTTDGLGLTGR
MLBr_00322|M.leprae_Br4923          EVVNASVQVIGNRPKIGPRRTEAQRLLSALLRAPVSVAATTTEGLGLTGR
MAV_0572|M.avium_104                RVGNAAVQVIGNRPKVGPRRAEAQRVLSELLGAPVSVAATTTDGLGLTGR
                                     : ** ***:*****:* ** *** :*: :: *****:****:*******

MSMEG_6075|M.smegmatis_MC2_155      GEGLAAIATALVAAEPVGDAK------
TH_1184|M.thermoresistible__bu      GEGLAAIATALLVGGAVGGENRPAVGR
MAB_0570|M.abscessus_ATCC_1997      GEGLAAVATALVTAL------------
Mflv_1445|M.gilvum_PYR-GCK          GEGLAAIATALVLAPADA---------
Mvan_5339|M.vanbaalenii_PYR-1       GEGLAAIATALVVGDEGKG--------
Mb3612c|M.bovis_AF2122/97           GEGLAAIATALVVSLR-----------
Rv3581c|M.tuberculosis_H37Rv        GEGLAAIATALVVSLR-----------
MMAR_5081|M.marinum_M               GEGLAAIATALVVSLRDPLGSQ-----
MUL_4157|M.ulcerans_Agy99           GEGLAAIATALVVSLRDPLGSQ-----
MLBr_00322|M.leprae_Br4923          GEGLSAIATALVVQV------------
MAV_0572|M.avium_104                GEGLAAIATALVVPTG-----------
                                    ****:*:****:

species	id	gene	e-value	identity (len)	annotation
M. leprae Br4923	MLBr_00322	ispF	-	100% (158)	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase