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QIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHIHVVMWAARRSG LYYVPINTHLTAAEAAYIVDNSSARAIVGSAALRDTCARLGEHLPGGLPDLLLMADGDLDGWERYPECVA GEPDTPIDDELEGDLLQYSSGTTGRPKGIKRELPHVHPAEAPGMMSALVGFWMTPESIYLSPAPLYHTAP SVWSMSAQAGGITTVVMEKFDAEGCLDAIQRHRVTHGQFVPAMFTRMLKLPEAVRHSYDLSSLQRVMHAA APCPVEIKKQMIDWWGPIIDEYYASSEAIGSTLISAEEWLAHPGSVGKPMACEIHILDENGNELPPGQAG EIYFSGGYSFEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRRHHMIISGGVNIYPQEAENLLV THPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAIAFEEQLPRTDT GKLFKNGLIEKYSV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. avium 104 | MAV_4961 | - | - | 100% (505) | acyl-CoA synthetase |
| M. avium 104 | MAV_2547 | - | 0.0 | 72.67% (505) | acyl-CoA synthetase |
| M. avium 104 | MAV_2951 | - | e-132 | 47.43% (506) | acyl-CoA synthase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0220 | fadD4 | 0.0 | 80.79% (505) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0449 | - | 0.0 | 76.55% (516) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv0214 | fadD4 | 0.0 | 80.99% (505) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 2e-55 | 30.51% (495) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_3435 | - | 1e-131 | 49.30% (501) | acyl-CoA synthetase |
| M. marinum M | MMAR_0452 | fadD4 | 0.0 | 83.60% (506) | fatty-acid-CoA ligase FadD4 |
| M. smegmatis MC2 155 | MSMEG_0257 | - | 0.0 | 82.54% (504) | acyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_4613 | - | 0.0 | 81.07% (507) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_1191 | fadD2 | 7e-56 | 30.86% (486) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0218 | - | 0.0 | 77.71% (516) | acyl-CoA synthetase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0220|M.bovis_AF2122/97 ------------------------MPRGELYKRFRLVMGGIAPCGSGRRA
Rv0214|M.tuberculosis_H37Rv ------------------------MPRGELYKRFRLVMGGIAPCGSGRRA
MMAR_0452|M.marinum_M --------------------------------------------------
MAV_4961|M.avium_104 --------------------------------------------------
MSMEG_0257|M.smegmatis_MC2_155 --------------------------------------------------
Mflv_0449|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_0218|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_4613|M.thermoresistible__bu --------------------------------------------------
MAB_3435|M.abscessus_ATCC_1997 --------------------------------------------------
MLBr_02546|M.leprae_Br4923 ---------MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVA
MUL_1191|M.ulcerans_Agy99 MPNLSDLPGVAKIQQYVDRGAAELHYVRKIIESGAFRLESPLNYAAMATE
Mb0220|M.bovis_AF2122/97 ATYPRRMQIRPYIAAD--KPAVILYPSGTVISFDELEARANRLAHWFRQA
Rv0214|M.tuberculosis_H37Rv ATYPRRMQIRPYIGAD--KPAVILYPSGTVISFDELEARANRLAHWFRQA
MMAR_0452|M.marinum_M ------MQIRQHVGAD--KPAVILHPSGTVVSFDELEARANRLAHRFRRA
MAV_4961|M.avium_104 ------MQIREYLGAG--KPAVILYPSGTVVTFDDLEARANRLAHRFRKA
MSMEG_0257|M.smegmatis_MC2_155 ------MQIREHVGAN--KPAIILYPSGTVVTFDELEARANRLAHHFRNQ
Mflv_0449|M.gilvum_PYR-GCK ------MQIRDTAVATPDKPAVIMYPAGTVVTFGELEARANRLAHLFRDA
Mvan_0218|M.vanbaalenii_PYR-1 ------MQIRDTAVATPDKPAIIMYPSGTVVTFGELEARANRLAHLFRDA
TH_4613|M.thermoresistible__bu ------MQIREHAEATPDKPAVILYPSDKVVTFGELEARANRLAHFFREN
MAB_3435|M.abscessus_ATCC_1997 ------MYPGAHAAQFPDKPAVVMAGSGATLTYGELDRQSRRLARHWYDS
MLBr_02546|M.leprae_Br4923 MRKWGDLAMLPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAK
MUL_1191|M.ulcerans_Agy99 IAKWGEFGMLPSFNARRHPHRAACIDEEGEFTYQQLDEAGHAVANALLAK
: .:: :*: .. :*.
Mb0220|M.bovis_AF2122/97 GLREDDVVAILMENNEHVHAVMWAARRSGLYYVPINTHLTASEAAYIVDN
Rv0214|M.tuberculosis_H37Rv GLREDDVVAILMENNEHVHAVMWAARRSGLYYVPINTHLTASEAAYIVDN
MMAR_0452|M.marinum_M GLREGDAVAILMENNEHIHAVMWAARRSGLYYVPINTHLTAAEAAYIIEN
MAV_4961|M.avium_104 GLREGDTVAILMENNEHIHVVMWAARRSGLYYVPINTHLTAAEAAYIVDN
MSMEG_0257|M.smegmatis_MC2_155 GLQEGDVVAILMENNSHIHTVMWAARRSGLYYVPISTHLTAAEAAYIVDN
Mflv_0449|M.gilvum_PYR-GCK GLVEGDAVALLMENNEHFHAVMWAARRAGLYYVPINTHLTAAEVAYIVDN
Mvan_0218|M.vanbaalenii_PYR-1 GLVEGDAVAILMENNEHIHAVMWAARRAGLYYVPISTHLTAAEVAYIVDN
TH_4613|M.thermoresistible__bu GLREGDTVAILMENNDHIFTAMWAARRSGLYYVPINTHLTAAEAAYIIDN
MAB_3435|M.abscessus_ATCC_1997 GLRKGDHVALLSDNVPEVFVVYWAALRSGLYITSVNHHLAPAEVSYIVND
MLBr_02546|M.leprae_Br4923 GVRGGDGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSER
MUL_1191|M.ulcerans_Agy99 GVSAGDGVAILARNHRWFLIANYGAARAGARIILLNSEFSGPQIKEVSER
*: .* :*:* * . . :.. * * :. .:: .: : :
Mb0220|M.bovis_AF2122/97 SGAKAIVGSAALRETCHGLAEHLPGGLPDLLMLAG-----------GGLV
Rv0214|M.tuberculosis_H37Rv SGAKAIVGSAALRETCHGLAEHLPGGLPDLLMLAG-----------GGLV
MMAR_0452|M.marinum_M SGAKAIVGSAALRDTCAGLGEHLRAGPPELLILAG---------PEGCLT
MAV_4961|M.avium_104 SSARAIVGSAALRDTCARLGEHLPGGLPDLLLMAD-----------GDLD
MSMEG_0257|M.smegmatis_MC2_155 SGAKAIVGSAKMRDVLAGLGDELPNGLPDTLLIAD-----------GDLD
Mflv_0449|M.gilvum_PYR-GCK SGAKAIVGSAKLADTLAGLEAELPNGLPQLLLVAG-AELSATSASGGDVD
Mvan_0218|M.vanbaalenii_PYR-1 SGAKAIVGSAKLKDTLAGLAEELPNGLPPLRLIAGGAESSATSASS-ELE
TH_4613|M.thermoresistible__bu SAAKAIIGSAALRDTLADLGEHLPGGLPELRMITD-----------SDLP
MAB_3435|M.abscessus_ATCC_1997 SGSSALVVAAGVREQAEAILDDTP--AVTLRLAFG-----------GDVR
MLBr_02546|M.leprae_Br4923 EGAKVIIYDDEYTKVVS-KAEPALGKLRALGVNPD--------EEEPPGS
MUL_1191|M.ulcerans_Agy99 EGAKLIIYDDEYTGAVS-KAEPPLGKLRALGVNPD--------SEEDSGS
..: :: : .
Mb0220|M.bovis_AF2122/97 GWMTYPECVADQPDTPIEDE-REGDLLQYSSGTTGRPKGIKRELPHVSPD
Rv0214|M.tuberculosis_H37Rv GWMTYPECVADQPDTPIEDE-REGDLLQYSSGTTGRPKGIKRELPHVSPD
MMAR_0452|M.marinum_M GWEPYPQCVADQPDTAIDDE-LEGDLLQYSSGTTGRPKGIKRELLHVAPD
MAV_4961|M.avium_104 GWERYPECVAGEPDTPIDDE-LEGDLLQYSSGTTGRPKGIKRELPHVHPA
MSMEG_0257|M.smegmatis_MC2_155 GWLRYPEAVADLPDTPIDDE-LDGDLLQYSSGTTGRPKGIKRELPHVHPS
Mflv_0449|M.gilvum_PYR-GCK GWQRYPECVADKLETPIADE-IDGDLLQYSSGTTGRPKGIKRELPHLPPA
Mvan_0218|M.vanbaalenii_PYR-1 GWQRYPECVADKPDTPIADE-IEGDLLQYSSGTTGRPKGIKRELPHVPPS
TH_4613|M.thermoresistible__bu GWQRYPECVAGQPDTPIADE-LEGDLLQYSSGTTGRPKGIKRELPHVPPS
MAB_3435|M.abscessus_ATCC_1997 GYDSYEDALDGASDEPLPAQ-PAGSDMLYSSGTTGRPKGIKVPLTDRQVD
MLBr_02546|M.leprae_Br4923 TDETLAELIARSSSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAP
MUL_1191|M.ulcerans_Agy99 TDETLAELIARSSTAPAPKAGKHASIIILTSGTTGTPKGANRSTPPTLAP
: : . .. : :***** *** :
Mb0220|M.bovis_AF2122/97 AAPGMMPALLDFWMDA-DSVYLSPAPMYHTAPSVWTMSALAAGVTTVVME
Rv0214|M.tuberculosis_H37Rv AAPGMMPALLDFWMDA-DSVYLSPAPMYHTAPSVWTMSALAAGVTTVVME
MMAR_0452|M.marinum_M AAPGMMSALVGYWMDA-DSIYLSPAPLYHTAPSVWSMSAQAGGITTVVLE
MAV_4961|M.avium_104 EAPGMMSALVGFWMTP-ESIYLSPAPLYHTAPSVWSMSAQAGGITTVVME
MSMEG_0257|M.smegmatis_MC2_155 ENPGMMAALISFWMTP-DSVYLSPAPLYHTAPSVWSMQVQAAGHTTVVME
Mflv_0449|M.gilvum_PYR-GCK EVPGMMAMLVEFWMHP-DAVYLSPAPLYHTAPSVWSMQVQAGGITTVVME
Mvan_0218|M.vanbaalenii_PYR-1 ESPGLMAALVSFWMHP-DAVYLSPAPLYHTAPSVWSMQTQAGGITTVVME
TH_4613|M.thermoresistible__bu EAPGMLSMLVNVWMQPPDSVYLSPAPLYHTAPSMWSISAQAAGVTVVVLE
MAB_3435|M.abscessus_ATCC_1997 EPNPFAMLFAHLYGFNAETVYLSPAPIYHAAPLRFCGFVQSMGGTAVVME
MLBr_02546|M.leprae_Br4923 -----LGGILSKVPFRAHEVTLLPAPMFHALGYLHATLAMFLGSTLVLRR
MUL_1191|M.ulcerans_Agy99 -----VGGILSHVPFKANEVTSLPAPMFHALGYLHATIAMFLGSTLVLRR
: . : ***::*: . * * *: .
Mb0220|M.bovis_AF2122/97 KFDAEGALDAIQRYRVTHAQFVPAMFVRMLKLPEAVRNSYDMSSLRRVIH
Rv0214|M.tuberculosis_H37Rv KFDAEGALDAIQRYRVTHAQFVPAMFVRMLKLPEAVRNSYDMSSLRRVIH
MMAR_0452|M.marinum_M KFDPEGALEAIQRHRVTHAQFVPAMFTRMLKLPESIRNSYDVSSLKRVMH
MAV_4961|M.avium_104 KFDAEGCLDAIQRHRVTHGQFVPAMFTRMLKLPEAVRHSYDLSSLQRVMH
MSMEG_0257|M.smegmatis_MC2_155 KFDAEGCLEAIQKHRVTHGQFVPAMFTRMLKLPESVRNSYDVSSLKRVMH
Mflv_0449|M.gilvum_PYR-GCK KFDAEGALDAIQKYKVTHGQFVPVMFARMLKLPEDVRKSYDVSSLQRVMH
Mvan_0218|M.vanbaalenii_PYR-1 KFDAEGALDAIAKHKVTHGQFVPVMFTRMLKLPEAVRKSYDVSSLERVMH
TH_4613|M.thermoresistible__bu KFDPERCLEAIQKYRVTHGQFVPVMFTRMLKLPESVRNSYDVSSLKRVIH
MAB_3435|M.abscessus_ATCC_1997 RFEAEEALAAIEKYRITHSQWVPTMFVRMLKLPKEIRDRYDVSSLKVAVH
MLBr_02546|M.leprae_Br4923 RFKPATVLEDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFV
MUL_1191|M.ulcerans_Agy99 KFKPQLVLEDIEKHKVTAMVVVPVMLSRLLDAVEKIDKKPDLSSLKIVFV
:*.. * * ::: * **.*: *:*. : : *:***. ..
Mb0220|M.bovis_AF2122/97 AAAPCPVQIKEQMIHWWGPIIDEYYASSEASGSTLITAEDWLTHPGSVGK
Rv0214|M.tuberculosis_H37Rv AAAPCPVQIKEQMIHWWGPIIDEYYASSEASGSTLITAEDWLTHPGSVGK
MMAR_0452|M.marinum_M AAAPCPVEIKKQMMDWWGPIIDEYYASSEAIGSTLITAEDWLEHPGSVGK
MAV_4961|M.avium_104 AAAPCPVEIKKQMIDWWGPIIDEYYASSEAIGSTLISAEEWLAHPGSVGK
MSMEG_0257|M.smegmatis_MC2_155 AAAPCPVDIKKQMIDWWGPIVDEYYASSEAIGATLITAEEWLKHPGSVGK
Mflv_0449|M.gilvum_PYR-GCK AAAPCPVEIKKQMIDWWGPIVDEYYASSEAHGSTLITADQWLERPGSVGK
Mvan_0218|M.vanbaalenii_PYR-1 AAAPCPVDIKKQMIDWWGPIVDEYYASSEAHGSTLISAEDWLTHPGSVGK
TH_4613|M.thermoresistible__bu AAAPCPVEIKKQMIDWWGPIIDEYYASSEAHGSTLISAEEWLQHPGSVGK
MAB_3435|M.abscessus_ATCC_1997 AAAPCPVEVKQSMIDWWGPILYEYYSSTEGNGVTFIDCATWLKRPGSVGS
MLBr_02546|M.leprae_Br4923 SGSQLGAELATRALEELGPVIYNMYGSTEIAFATIAGPKDLQINSATVGP
MUL_1191|M.ulcerans_Agy99 SGSQLGAELASRALKDLGPVIYNMYGSTEIAFATIAGPEDLQRNAATVGP
:.: .:: :. **:: : *.*:* *: ...:**
Mb0220|M.bovis_AF2122/97 PIQGG-VHIVGADGSELPPNQPGEIYFEGG-YPFEYLNDPAKTAASRNKH
Rv0214|M.tuberculosis_H37Rv PIQGG-VHIVGADGSELPPNQPGEIYFEGG-YPFEYLNDPAKTAASRNKH
MMAR_0452|M.marinum_M PMQGG-VHILGADGVELPPGQAGEIYFEGG-YSFEYLNDPSKTAASRDKH
MAV_4961|M.avium_104 PMACE-IHILDENGNELPPGQAGEIYFSGG-YSFEYLNDEAKTAASRDKH
MSMEG_0257|M.smegmatis_MC2_155 PLTGI-VHVLDEDGNELPPGQAGEIFFEGG-QDFEYLNDAEKTASSRDAR
Mflv_0449|M.gilvum_PYR-GCK PLTGV-VHIVGEDGNELPAGQAGEIYFEGG-YDFEYLNDPDKTASSRHPQ
Mvan_0218|M.vanbaalenii_PYR-1 PLTGV-VHIVGEDGEELPPGQAGEIYFEGG-YDFEYLNDPDKTASSRHPQ
TH_4613|M.thermoresistible__bu PMLGA-VHILDEDGNELPPGQPGEIYFEGG-NSFEYLNDPEKTAASRHPK
MAB_3435|M.abscessus_ATCC_1997 AALGV-LHICDETGAELPAGEAGLVYFEREELPFSYHNDDEKTRSACHPA
MLBr_02546|M.leprae_Br4923 VVKGVKVKILDDSGKEVPRGQVGRIFVGNA-FPFEGYTGGGGKQIIDGLL
MUL_1191|M.ulcerans_Agy99 VVKGVKVKILDDNGNEVSQGQVGRIFVGNA-FPFEGYTGGGHKQIIDGLM
::: . * *:. .: * ::. *. .. .
Mb0220|M.bovis_AF2122/97 G--WVTVGDVGYLDDDGYLFLTGRRHHMIISGGVNIYPQEAENLLVAHPK
Rv0214|M.tuberculosis_H37Rv G--WVTVGDVGYLDDDGYLFLTGRRHHMIISGGVNIYPQEAENLLVAHPK
MMAR_0452|M.marinum_M G--WVTVGDVGYLDDQGYLFLTDRRHHMIISGGVNIYPQEAENILVTHPK
MAV_4961|M.avium_104 G--WVTVGDVGYVDEEGYLYLTDRRHHMIISGGVNIYPQEAENLLVTHPK
MSMEG_0257|M.smegmatis_MC2_155 G--WKTVGDIGYLDEEGYLYLTDRRHHMIISGGVNIYPQEAENMLVTHPK
Mflv_0449|M.gilvum_PYR-GCK G--WKTVGDIGYVDEEGFLFLTDRRHHMIISGGVNIYPQEAENLLVTHPK
Mvan_0218|M.vanbaalenii_PYR-1 G--WKTVGDIGYLDDEGYLYLTDRRHHMIISGGVNIYPQEAENLLVTHPK
TH_4613|M.thermoresistible__bu G--WVTVGDVGYVDEDGYLYLTDRRHHMIISGGVNIYPQEAENMLVTHPK
MAB_3435|M.abscessus_ATCC_1997 HDTWTTTGDIGYVDEDGFLYLTDRKAFMIISGGVNIYPQEIENALALHPA
MLBr_02546|M.leprae_Br4923 S-----SGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPE
MUL_1191|M.ulcerans_Agy99 S-----SGDVGYFDEHGLLYVSGRDDEMIVSGGENVFPAEVEALISGHPD
**:**.*:.. *:::.* **:*** *::* * * : **
Mb0220|M.bovis_AF2122/97 VLDAAVFGVPDDEMGQRVMAAVQTVDSADANDQFAGELLAWLRDRLSHFK
Rv0214|M.tuberculosis_H37Rv VLDAAVFGVPDDEMGQRVMAAVQTVDSADANDQFAGELLAWLRDRLSHFK
MMAR_0452|M.marinum_M VMDAAVFGVPDDEMGQRVMAVVQTVDPADATSQFGDELMEWLRDRLSHFK
MAV_4961|M.avium_104 VLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYK
MSMEG_0257|M.smegmatis_MC2_155 VMDAAVFGVPDDEMGQSVKGVVQTVDPADATPEFGEELLAWLRDRLAHYK
Mflv_0449|M.gilvum_PYR-GCK VMDAAVFGIPDEEMGQRVKAVVQLVDQADATEEFADELIGWLRDRLAHYK
Mvan_0218|M.vanbaalenii_PYR-1 VMDAAVFGIPDEEMGQSVKAVVQTVDPADATEEFAAELISWLRDRLAHYK
TH_4613|M.thermoresistible__bu VMDAAVFGVPDEEMGERVKAVVQLVDPSEASDELAAELIEWLRDRLAHYK
MAB_3435|M.abscessus_ATCC_1997 VLDVAVIGVPDAEMGESVKAVVQPADSSAAPRELAAELTDFLRERIARYK
MLBr_02546|M.leprae_Br4923 VVEATAIGVDDKEWGARLRAFVVKKQDATIDEDA---IKAYVRDHLARYK
MUL_1191|M.ulcerans_Agy99 VVEATALGVDDKEWGARLRAFVVKKPDTDLDEET---VKHYVRDHLARYK
*::.:.:*: * * * : . * : : ::*::::::*
Mb0220|M.bovis_AF2122/97 CPRSIAFEPQLPRTDTGKLYKSGLVEKYSV---
Rv0214|M.tuberculosis_H37Rv CPRSIAFEPQLPRTDTGKLYKSGLVEKYSV---
MMAR_0452|M.marinum_M CPRSVAFEAQLPRTDTGKLYKNGLIEKYSG---
MAV_4961|M.avium_104 CPRAIAFEEQLPRTDTGKLFKNGLIEKYSV---
MSMEG_0257|M.smegmatis_MC2_155 CPRSISFELQLPRTDTGKLYKQELIAKYS----
Mflv_0449|M.gilvum_PYR-GCK CPRSLSFEEQLPRTDTGKLYKQELIKKYS----
Mvan_0218|M.vanbaalenii_PYR-1 CPRSLSFEAQLPRTDTGKLYKQELVKKHS----
TH_4613|M.thermoresistible__bu CPRSISFEEQLPRTDTGKLYKQELIKKYS----
MAB_3435|M.abscessus_ATCC_1997 VPRSFDFTDTLPRTPTGKLAKGLLRQKYWEGAQ
MLBr_02546|M.leprae_Br4923 VPREVIFLEELPRNPTGKVLKRELRDFEVH---
MUL_1191|M.ulcerans_Agy99 VPREVIFLEELPRNPTGKILKRELREMEIE---
** . * ***. ***: * *