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aa seq not available
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. thermoresistible (build 8) | TH_1783 | - | 100% (513) | CONSERVED HYPOTHETICAL ALANINE, ARGININE-RICH PROTEIN |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2752c | - | 0.0 | 81.84% (501) | hypothetical protein Mb2752c |
| M. gilvum PYR-GCK | Mflv_3967 | - | 0.0 | 82.64% (507) | RNA modification protein |
| M. tuberculosis H37Rv | Rv2733c | - | 0.0 | 81.84% (501) | hypothetical protein Rv2733c |
| M. leprae Br4923 | MLBr_00989 | - | 0.0 | 77.08% (506) | (dimethylallyl)adenosine tRNA methylthiotransferase |
| M. abscessus ATCC 19977 | MAB_3048c | - | 0.0 | 77.98% (504) | putative tRNA-i(6)A37 modification enzyme MiaB |
| M. marinum M | MMAR_1979 | - | 0.0 | 80.43% (506) | hypothetical protein MMAR_1979 |
| M. avium 104 | MAV_3625 | - | 0.0 | 81.97% (488) | hypothetical protein MAV_3625 |
| M. smegmatis MC2 155 | MSMEG_2729 | miaB | 0.0 | 84.80% (513) | tRNA-I(6)A37 thiotransferase enzyme MiaB |
| M. ulcerans Agy99 | MUL_3378 | - | 0.0 | 80.43% (506) | hypothetical protein MUL_3378 |
| M. vanbaalenii PYR-1 | Mvan_2429 | - | 0.0 | 84.06% (502) | RNA modification protein |
CLUSTAL 2.0.9 multiple sequence alignment
TH_1783|M.thermoresistible__bu --MVTRSRTGAGGASGAARERSSARTYQIRTYGCQMNVHDSERLAGLLEA
MSMEG_2729|M.smegmatis_MC2_155 --MVTRDETAEAEGLRPAVGSSRVRTYQVRTYGCQMNVHDSERLSGLLES
Mflv_3967|M.gilvum_PYR-GCK ----MLQQADGVSPDRSSCDTPAPRTFEVRTYGCQMNVHDSERLSGLLEQ
Mvan_2429|M.vanbaalenii_PYR-1 MTSTVTQQASGPSPDRS------ARTFEVRTYGCQMNVHDSERLAGLLEG
MMAR_1979|M.marinum_M -MTSTVAHGAGSAGPADDAEPMSARTYQVRTYGCQMNVHDSERMAGLLEA
MUL_3378|M.ulcerans_Agy99 -MTSTVAHGAGSAGPADDVEPMSARTYQVRTYGCQMNVHDSERMAGLLEA
Mb2752c|M.bovis_AF2122/97 MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEA
Rv2733c|M.tuberculosis_H37Rv MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEA
MAV_3625|M.avium_104 -----------------------MRTYQVRTYGCQMNVHDSERLAGLLEA
MLBr_00989|M.leprae_Br4923 MVTRDAGMADDAATG-------STRTYQVRTYGCQMNVHDSERLAGLLEA
MAB_3048c|M.abscessus_ATCC_199 -MTSTATSGES-----------AVRTYQVRTYGCQMNVHDSERVSGLLDA
**:::**************::***:
TH_1783|M.thermoresistible__bu AGYRRASDGAD---ADVVVFNTCAVRENADNKLYGNLSHLAPRKQADPDL
MSMEG_2729|M.smegmatis_MC2_155 AGYQRAAEGTD---ADIVVFNTCAVRENADNKLYGNLSHLAPRKQADPNM
Mflv_3967|M.gilvum_PYR-GCK AGYQRAGAGVD---ADIVVFNTCAVRENADNKLYGNLSHLAPRKQADPQM
Mvan_2429|M.vanbaalenii_PYR-1 AGYRRAGEGAD---ADIVVFNTCAVRENADNKLYGNLSHLAPRKQSDPDM
MMAR_1979|M.marinum_M AGYRRAAEGTD---ADVVVFNTCAVRENADNKLYGNLSHLAPRKRTSPDM
MUL_3378|M.ulcerans_Agy99 AGYRRAAEGTD---ADVVVFNTCAVRENADNKLYGNLSHLAPRKRTSPDM
Mb2752c|M.bovis_AF2122/97 AGYRRATDGSE---ADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDM
Rv2733c|M.tuberculosis_H37Rv AGYRRATDGSE---ADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDM
MAV_3625|M.avium_104 AGYRRAAEGAEV--ADVVVFNTCAVRENADNKLYGNLSHLAPRKRSNPQM
MLBr_00989|M.leprae_Br4923 AGYQRAADEADVGDADVVVFNTCAVRENADNRLYGNLSHLAPRKRNNPDM
MAB_3048c|M.abscessus_ATCC_199 AGYVKAPEGTD---ADIVIFNTCAVRENADNKLYGNISHLAPRKAANPNM
*** :* : **:*:************:****:******* .*::
TH_1783|M.thermoresistible__bu QIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPALLERARHNRTAQV
MSMEG_2729|M.smegmatis_MC2_155 QIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNREAQV
Mflv_3967|M.gilvum_PYR-GCK QIAVGGCLAQKDRDAVLRKAPWVDVVFGTHNIGSLPTLLDRARHNRVAQV
Mvan_2429|M.vanbaalenii_PYR-1 QIAVGGCLAQKDRDSVLRKAPWVDVVFGTHNIGSLPALLDRARHNRVAQV
MMAR_1979|M.marinum_M QIAVGGCLAQKDRDALLRKAPWVDVVFGTHNIGSLPALLDRARHNRVAQV
MUL_3378|M.ulcerans_Agy99 QIAVGGCLAQKDRDALLRKAPWVDVVFGTHNIGSLPALLDRARHNRVAQV
Mb2752c|M.bovis_AF2122/97 QIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQV
Rv2733c|M.tuberculosis_H37Rv QIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQV
MAV_3625|M.avium_104 QIAVGGCLAQKDREAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKAAQV
MLBr_00989|M.leprae_Br4923 QIAVGGCLAQKDKHTVLSKAPWVDIVFGTHNLGSLPTLLDRARHNKVAQV
MAB_3048c|M.abscessus_ATCC_199 QIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSLPALLERARHNNEAQV
************:. :* :*****:******:****:**:*****. ***
TH_1783|M.thermoresistible__bu EIAEALQEFPSNLPAARESAYAAWVSISVGCNNTCTFCIVPALRGKEVDR
MSMEG_2729|M.smegmatis_MC2_155 EIVEALQEFPSALPASRESAYAAWVSISVGCNNTCTFCIVPSLRGKEVDR
Mflv_3967|M.gilvum_PYR-GCK EIAESLREFPSALPASRESAYAAWVSISVGCNNTCTFCIVPSLRGKEVDR
Mvan_2429|M.vanbaalenii_PYR-1 EIAEALQEFPSALPASRESSYAAWVSISVGCNNTCTFCIVPALRGREVDR
MMAR_1979|M.marinum_M EIAEALQQFPSSLPSARESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDR
MUL_3378|M.ulcerans_Agy99 EIAEALQQFPSSLPSARESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDR
Mb2752c|M.bovis_AF2122/97 EIAEALQQFPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDR
Rv2733c|M.tuberculosis_H37Rv EIAEALQQFPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDR
MAV_3625|M.avium_104 EIAEALQQFPSSLPSARESAYAAWVSISVGCNNSCTFCIVPSLRGKEVDR
MLBr_00989|M.leprae_Br4923 EIVEALQHFPSSLPSARESDYAAWVSISVGCNNSCTFCIVPSLRGKEVDR
MAB_3048c|M.abscessus_ATCC_199 EIVEALEHFPSALPATRESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDR
**.*:*..*** **::*** *************:*******:***:*:**
TH_1783|M.thermoresistible__bu RPDDILAEVRALVDQGVLEVTLLGQNVNAYGVSFADPDLPRHRGAFADLL
MSMEG_2729|M.smegmatis_MC2_155 RPGDILAEVQSLVDQGVLEITLLGQNVNAYGVSFADPELPRDRGAFAKLL
Mflv_3967|M.gilvum_PYR-GCK RPGDVLAEVQALVDQGVLEITLLGQNVNAYGVSFADPTEARDRGAFAKLL
Mvan_2429|M.vanbaalenii_PYR-1 RPGDVLAEVQSLVDQGVLEITLLGQNVNAYGVSFADPTEPRDRGAFAKLL
MMAR_1979|M.marinum_M SPADILAEVQSLVDTGVVEITLLGQNVNAYGVSFADPALPRNRGAFAELL
MUL_3378|M.ulcerans_Agy99 SPADILAEVQSLVDTGVVEITLLGQNVNAYGVSFADPALPRNRGAFAELL
Mb2752c|M.bovis_AF2122/97 SPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELL
Rv2733c|M.tuberculosis_H37Rv SPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELL
MAV_3625|M.avium_104 SPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPALPRDRGAFARLL
MLBr_00989|M.leprae_Br4923 SPADILAEVEALVADGVLEVTLLGQNVNAYGVSFADPALARNRGSFAELL
MAB_3048c|M.abscessus_ATCC_199 RPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELL
* *:*.**.:** **:*:************.**** .*.**:** **
TH_1783|M.thermoresistible__bu RACGEIDGLERVRFTSPHPAEFTDDVIEAMAETPNVCPALHMPLQSGSDR
MSMEG_2729|M.smegmatis_MC2_155 RACGGIDGLERVRFTSPHPAEFTDDVIEAMAATPNVCPTLHMPLQSGSDR
Mflv_3967|M.gilvum_PYR-GCK RACGRIDGLERVRFTSPHPAEFTDDVIEAMAQTSNVCPTLHMPLQSGSDR
Mvan_2429|M.vanbaalenii_PYR-1 RACGRIDGLERVRFTSPHPAEFTDDVIEAMAETPNVCPTLHMPLQSGSDR
MMAR_1979|M.marinum_M RACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDR
MUL_3378|M.ulcerans_Agy99 RACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDR
Mb2752c|M.bovis_AF2122/97 RACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDR
Rv2733c|M.tuberculosis_H37Rv RACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDR
MAV_3625|M.avium_104 RACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDR
MLBr_00989|M.leprae_Br4923 RSCGSIDGLERVRFTSPHPAEFTYDVIEAMAQTPNVCPSLHMPLQSGSDR
MAB_3048c|M.abscessus_ATCC_199 RACGRIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDR
*:** ****************** ******* * **** ***********
TH_1783|M.thermoresistible__bu ILRAMRRSYRAERFLGILDKVRAAIPHAAITTDLIVGFPGETEEDFQATL
MSMEG_2729|M.smegmatis_MC2_155 ILKAMRRSYRAERFLGIIDKVRAAIPHAAITTDLIVGFPGETEEDFQATL
Mflv_3967|M.gilvum_PYR-GCK ILRAMRRSYRADRYLGIIDRVRTAIPHAAITTDLIVGFPGETEEDFQATL
Mvan_2429|M.vanbaalenii_PYR-1 ILRAMRRSYRAERYLGIIDRVRAAIPHAAITTDLIVGFPGETEEDFQATL
MMAR_1979|M.marinum_M VLRAMRRSYRAERYLGIIERVRAAMPHAAITTDLIVGFPGETEQDFAATL
MUL_3378|M.ulcerans_Agy99 VLRAMRRSYRAERYLGIIERVRAAMPHAAITTDLIVGFPGETEQDFAATL
Mb2752c|M.bovis_AF2122/97 ILRAMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATL
Rv2733c|M.tuberculosis_H37Rv ILRAMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATL
MAV_3625|M.avium_104 VLRAMRRSYRAERYLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATL
MLBr_00989|M.leprae_Br4923 VLRAMRRSYRVERYLGIIGQVRAAMPHAAITTDLIVGFPGETEEDFAATL
MAB_3048c|M.abscessus_ATCC_199 ILKAMRRSYRSERFLSIIDKVRSAMPDAAITTDIIVGFPGETEEDFQQTL
:*:******* :*:*.*: :**:*:*.******:*********:** **
TH_1783|M.thermoresistible__bu DVVEQARFAGAFTFQYSRRPGTPAADLPDQLPKAVVQERYERLIALQDRI
MSMEG_2729|M.smegmatis_MC2_155 DVVEQARFAGAFTFQYSKRPGTPAADMPDQLPKSVVTERYQRLIELQERI
Mflv_3967|M.gilvum_PYR-GCK DVVEAARFSSAFTFQYSKRPGTPAAELEGQIPKAVVSERYQRLIELQERI
Mvan_2429|M.vanbaalenii_PYR-1 DVVAASRFSSAFTFQYSKRPGTPAAELADQVPKAVVSERYQRLIELQERI
MMAR_1979|M.marinum_M DVVRQARFSAAFTFQYSKRPGTPAAELDGQLPKAVVQERYERLVELQEQI
MUL_3378|M.ulcerans_Agy99 DVVRQARFSAAFTFQYSKRPGTPAAELDGQLPKAVVQERYERLVELQEQI
Mb2752c|M.bovis_AF2122/97 DVVRRARFAAAFTFQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQI
Rv2733c|M.tuberculosis_H37Rv DVVRRARFAAAFTFQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQI
MAV_3625|M.avium_104 DVVRRARFAAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESI
MLBr_00989|M.leprae_Br4923 DVVRQVRFTAAFTFQYSKRPGTPAAELGGQLPKAVVQERYERLVELQEQI
MAB_3048c|M.abscessus_ATCC_199 EVVRRARFSSAFTFQYSIRPGTPAAKLPDQLPKAVVQERYDRLIALQESV
:** **:.******* *******.: .*:**:** ***:**: **: :
TH_1783|M.thermoresistible__bu ALEENTAQIGRAVELLVATGEGRKDASTARMSGRARDGRLVHFTPGGL--
MSMEG_2729|M.smegmatis_MC2_155 SLEQNREQVGRAVELLVATGEGRKDASTARMSGRARDGRLVHFLPGDN--
Mflv_3967|M.gilvum_PYR-GCK SWEENRAQIGREVELLVATGEGRKDAATARMSGRARDGRLVHFAPGALG-
Mvan_2429|M.vanbaalenii_PYR-1 SLEENTAQIGRRVELLVATGEGRKDAATARMSGRARDGRLVHFAPGAVG-
MMAR_1979|M.marinum_M SLEGNRAIVGQRVELLVATGEGRKDTLTARMSGRARDGRLVHFRAGDG--
MUL_3378|M.ulcerans_Agy99 SLEGNRAIVGQRVELLVATGEGRKDTLTARMSGRARDGRLVHFRAGDG--
Mb2752c|M.bovis_AF2122/97 SLEANRALVGQAVEVLVATGEGRKDTVTARMSGRARDGRLVHFTAGQP--
Rv2733c|M.tuberculosis_H37Rv SLEANRALVGQAVEVLVATGEGRKDTVTARMSGRARDGRLVHFTAGQP--
MAV_3625|M.avium_104 SLQGNQALVGQTVELLVATGEGRKDSATARMSGRARDGRLVHFAADD---
MLBr_00989|M.leprae_Br4923 CMEENRVLIGRIVELLVTTGEGRKDARTARRTGRARDGRLVHFALDTPWE
MAB_3048c|M.abscessus_ATCC_199 TLEENQKQIGRMIEVLIATGEGRKDGETARMSGRARDGRLVHFRPQGHVD
: * :*: :*:*::******* *** :***********
TH_1783|M.thermoresistible__bu -DIRPGDIVTTTVTAAAPHYLIADAEPAGHRRTRAGDAHAAG--------
MSMEG_2729|M.smegmatis_MC2_155 -EIRPGDIVTTTVTGAAPHHLIADAPIIERRRTRAGDAHAAG--------
Mflv_3967|M.gilvum_PYR-GCK ADIRPGDIVVTTVTGAAPHHLIADAGPAEHRRTRAGDAHAEG--------
Mvan_2429|M.vanbaalenii_PYR-1 SEVRPGDVVVTTVTGAAPHHLIADAGLIEHRRTRAGDAHAAG--------
MMAR_1979|M.marinum_M -PVRPGDIVTVEVTDAAPHHLIADGGILAHRRTRAGDAHADG--------
MUL_3378|M.ulcerans_Agy99 -PVRPGDIVTVEVTDAAPHHLIADGGILAHRRTRAGDAHADG--------
Mb2752c|M.bovis_AF2122/97 -RVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAGDAHTAG--------
Rv2733c|M.tuberculosis_H37Rv -RVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAGDAHTAG--------
MAV_3625|M.avium_104 -RVRPGDLVTTVITGAAPHHLIADAGILSHRRTRAGDAHAPAGARAASVS
MLBr_00989|M.leprae_Br4923 AQVRPGDIITVMVTEAAPHHLIADAGILTHRRTRAGDAHAAR--------
MAB_3048c|M.abscessus_ATCC_199 GALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAG--------
:****::.. :* ****:**** :*********
TH_1783|M.thermoresistible__bu --------------------------------------LRPKT-VG----
MSMEG_2729|M.smegmatis_MC2_155 --------------------------------------QKPRTGVG----
Mflv_3967|M.gilvum_PYR-GCK --------------------------------------RTPKTGVG----
Mvan_2429|M.vanbaalenii_PYR-1 --------------------------------------QKPRTGVG----
MMAR_1979|M.marinum_M ---------------------------------------QTVRGIG----
MUL_3378|M.ulcerans_Agy99 ---------------------------------------QTVRGIG----
Mb2752c|M.bovis_AF2122/97 --------QPG-------------------------------RAVG----
Rv2733c|M.tuberculosis_H37Rv --------QPG-------------------------------RAVG----
MAV_3625|M.avium_104 ACPPSGRRRPGPTRWMCLMNDDRNHDDPRLGALRTEIEAAERRVAGGIDP
MLBr_00989|M.leprae_Br4923 -------QCP--------------------------------RSIG----
MAB_3048c|M.abscessus_ATCC_199 -------------------------------------KKPSTPGIG----
*
TH_1783|M.thermoresistible__bu -------------------------------------LGLPRIGGPK---
MSMEG_2729|M.smegmatis_MC2_155 -------------------------------------LGMPRIGAPA---
Mflv_3967|M.gilvum_PYR-GCK -------------------------------------LGMPGIGAPE---
Mvan_2429|M.vanbaalenii_PYR-1 -------------------------------------LGMPAVGAPD---
MMAR_1979|M.marinum_M -------------------------------------LGMPGIGRPVVP-
MUL_3378|M.ulcerans_Agy99 -------------------------------------LGMPGIGRPVVP-
Mb2752c|M.bovis_AF2122/97 -------------------------------------LGMPGVGLP----
Rv2733c|M.tuberculosis_H37Rv -------------------------------------LGMPGVGLP----
MAV_3625|M.avium_104 GARGFVVSILVFVLLGSFILPHTGDVRGWDVLFGTHDAGAAAVALPSRVF
MLBr_00989|M.leprae_Br4923 --------------------------------LGQIPLGMPSVGQHS---
MAB_3048c|M.abscessus_ATCC_199 -------------------------------------LGMPAIGAPK---
* . :.
TH_1783|M.thermoresistible__bu ----------------------------------------------PDAE
MSMEG_2729|M.smegmatis_MC2_155 ----------------------------------------------PSAT
Mflv_3967|M.gilvum_PYR-GCK ----------------------------------------------PAPV
Mvan_2429|M.vanbaalenii_PYR-1 ----------------------------------------------PLPA
MMAR_1979|M.marinum_M ------------------------------------------VAAEATSC
MUL_3378|M.ulcerans_Agy99 ------------------------------------------VAAEATSC
Mb2752c|M.bovis_AF2122/97 -----------------------------------------------VSA
Rv2733c|M.tuberculosis_H37Rv -----------------------------------------------VSA
MAV_3625|M.avium_104 GWLALVFGVGFSTLALVTRRWALAWIALAGTAIAGAAGMLAIWSRQTVPA
MLBr_00989|M.leprae_Br4923 -----------------------------------------------ASI
MAB_3048c|M.abscessus_ATCC_199 ----------------------------------------------TERI
TH_1783|M.thermoresistible__bu PTRGCGC-------------------------------------------
MSMEG_2729|M.smegmatis_MC2_155 -AEGCGC-------------------------------------------
Mflv_3967|M.gilvum_PYR-GCK -TQGCAL-------------------------------------------
Mvan_2429|M.vanbaalenii_PYR-1 -TTGCAR-------------------------------------------
MMAR_1979|M.marinum_M GSAGGCG----------------------------SADGAGSSAGDPQ--
MUL_3378|M.ulcerans_Agy99 GSAGGCG----------------------------SADGAGSSAGDPQ--
Mb2752c|M.bovis_AF2122/97 AKPGGCR-------------------------------------------
Rv2733c|M.tuberculosis_H37Rv AKPGGCR-------------------------------------------
MAV_3625|M.avium_104 GHPGPGWGLIVAWITVLVLIYQWARVVWSRTIVQLAAEEQRRRVAAQQQS
MLBr_00989|M.leprae_Br4923 GSAGCAR-------------------------------------------
MAB_3048c|M.abscessus_ATCC_199 EVAGCGL-------------------------------------------
*
TH_1783|M.thermoresistible__bu -----------------
MSMEG_2729|M.smegmatis_MC2_155 -----------------
Mflv_3967|M.gilvum_PYR-GCK -----------------
Mvan_2429|M.vanbaalenii_PYR-1 -----------------
MMAR_1979|M.marinum_M -----------------
MUL_3378|M.ulcerans_Agy99 -----------------
Mb2752c|M.bovis_AF2122/97 -----------------
Rv2733c|M.tuberculosis_H37Rv -----------------
MAV_3625|M.avium_104 TTLLDDLPKPEDPAAGT
MLBr_00989|M.leprae_Br4923 -----------------
MAB_3048c|M.abscessus_ATCC_199 -----------------