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RVLVAMSGGVDSSVAAARMVAAGHDVVGVHLALSSAPGTLRTGSRGCCSKEDAGDARRVADVLGIPFYVW DFADRFKEDVIDDFVESYARGETPNPCVRCNERIKFSALASRALALGFDALATGHYARLADGRLRRAVDH DKDQSYVLAVLTAEQLRHAVFPIGDTPKAQIREEAARLGLAVADKADSHDICFIPSGDTKAFLGARIGVR RGNVVDAAGTVLAEHDGVHGFTIGQRKGLGIPGPGPDGSPRYVTGIDAQTGTVHVGDVADLEVTSLQGDA PVFTSGVAPTGPLECVVQVRAHGGVADAVAELRGDTLEVSLRTPLRGVAPGQTLVLYRRDPAGDEVLASA TIRATH*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4254 | mnmA | - | 100% (357) | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3050c | mnmA | 1e-166 | 81.30% (353) | tRNA-specific 2-thiouridylase MnmA |
| M. tuberculosis H37Rv | Rv3024c | mnmA | 1e-166 | 81.30% (353) | tRNA-specific 2-thiouridylase MnmA |
| M. leprae Br4923 | MLBr_01707 | mnmA | 1e-157 | 77.90% (353) | tRNA-specific 2-thiouridylase MnmA |
| M. abscessus ATCC 19977 | MAB_3356c | mnmA | 1e-153 | 75.64% (353) | tRNA-specific 2-thiouridylase MnmA |
| M. marinum M | MMAR_1690 | mnmA | 1e-170 | 83.71% (350) | tRNA (5-methylaminomethyl-2-thiouridylate)- |
| M. avium 104 | MAV_3871 | mnmA | 1e-164 | 81.43% (350) | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase |
| M. smegmatis MC2 155 | MSMEG_2358 | mnmA | 1e-174 | 85.55% (353) | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase |
| M. thermoresistible (build 8) | TH_1186 | - | 1e-172 | 84.14% (353) | PROBABLE tRNA |
| M. ulcerans Agy99 | MUL_1928 | mnmA | 1e-172 | 83.15% (356) | tRNA-specific 2-thiouridylase MnmA |
| M. vanbaalenii PYR-1 | Mvan_2106 | mnmA | 0.0 | 92.11% (355) | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_4254|M.gilvum_PYR-GCK MRVLVAMSGGVDSSVAAARMVAAGHDVVGVHLALSSAPGTLRTGSRGCCS
Mvan_2106|M.vanbaalenii_PYR-1 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTGSRGCCS
Mb3050c|M.bovis_AF2122/97 MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCS
Rv3024c|M.tuberculosis_H37Rv MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCS
MLBr_01707|M.leprae_Br4923 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTVSGTLRTGSRGCCS
MAV_3871|M.avium_104 ------MSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGTLRTGSRGCCS
MMAR_1690|M.marinum_M ------MSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGALRTGSRGCCS
MUL_1928|M.ulcerans_Agy99 MKVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSTAPGALRTGSRGCCS
TH_1186|M.thermoresistible__bu MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSEAPGTLRTGSRGCCS
MAB_3356c|M.abscessus_ATCC_199 MKILAAMSGGVDSSVAAARMVDAGHEVVGVHLALSAAPGTLRTGSRGCCS
MSMEG_2358|M.smegmatis_MC2_155 MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTGSRGCCS
*************** ***:*****:*** ..*:**********
Mflv_4254|M.gilvum_PYR-GCK KEDAGDARRVADVLGIPFYVWDFADRFKEDVIDDFVESYARGETPNPCVR
Mvan_2106|M.vanbaalenii_PYR-1 KEDAGDARRVADVLGIPFYVWDFADRFKEDVIDDFVESYARGETPNPCVR
Mb3050c|M.bovis_AF2122/97 KEDAADARRVADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVR
Rv3024c|M.tuberculosis_H37Rv KEDAADARRVADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVR
MLBr_01707|M.leprae_Br4923 KEDVSDARRVADVLGIPFYVWDFAEKFQEDVIDNFVSAYARGETPNPCVQ
MAV_3871|M.avium_104 KEDASDARRVADVLGIPFYVWDFAEKFQADVIDEFVSAYARGETPNPCVT
MMAR_1690|M.marinum_M KEDASDARRVADVLGIPFYVWDFAERFKEDVIDDFVSSYARGETPNPCIR
MUL_1928|M.ulcerans_Agy99 KEDASDARRVADVLGIPFYVWDFAERFKEDVIDDFVSSYARGETPNPCIR
TH_1186|M.thermoresistible__bu KEDAGDARRVADVLGIPFYIWDFAEKFKADVIDDFVSSYERGETPNPCVR
MAB_3356c|M.abscessus_ATCC_199 KEDAADARRVADMLGIPFYVWDFADRFKEDVIDDFVEAYAEGRTPNPCVK
MSMEG_2358|M.smegmatis_MC2_155 KEDASDARRVADILGIPFYVWDFADRFKDDVIDDFVESYARGETPNPCVR
***..*******:******:****::*: ***::**.:* .*.*****:
Mflv_4254|M.gilvum_PYR-GCK CNERIKFSALASRALALGFDALATGHYARLADGRLRRAVDHDKDQSYVLA
Mvan_2106|M.vanbaalenii_PYR-1 CNERIKFSALSARALALGFDALATGHYARLTDGRLRRAVDHDKDQSYVLA
Mb3050c|M.bovis_AF2122/97 CNQQIKFAALSARAVALGFDTVATGHYARLSGGRLRRAVDRDKDQSYVLA
Rv3024c|M.tuberculosis_H37Rv CNQQIKFAALSARAVALGFDTVATGHYARLSGGRLRRAVDRDKDQSYVLA
MLBr_01707|M.leprae_Br4923 CNQRIKFSALSVRALMLGFDTVATGHYARLSGGRLRRAVDRGKDQSYVLA
MAV_3871|M.avium_104 CNQKIKFSALSAKAVALGFDAVATGHYARLSGGRLRRAVDKDKDQSYVLA
MMAR_1690|M.marinum_M CNQRIKFSALYAKALALGFDVVVTGHYARLSEGRLRRAVDQDKDQSYVLA
MUL_1928|M.ulcerans_Agy99 CNQRIKFSALYAKALALGFDVVVTGHYARLSEGRLRRAVDQDKDQSYVLA
TH_1186|M.thermoresistible__bu CNERIKFSALAARAVALGFDAVATGHYARLTDGRLRRAVDRDKDQSYVLG
MAB_3356c|M.abscessus_ATCC_199 CNEKIKFAALADRAVALGFDAVATGHYARLQDGRLRRAVDADKDQSYVLG
MSMEG_2358|M.smegmatis_MC2_155 CNERIKFSALAARALALGFDAVATGHYARLSDGRLRRAVDADKDQSYVLG
**::***:** :*: ****.:.******* ******** .*******.
Mflv_4254|M.gilvum_PYR-GCK VLTAEQLRHAVFPIGDTPKAQIREEAARLGLAVADKADSHDICFIPSGDT
Mvan_2106|M.vanbaalenii_PYR-1 VLTAEQLRHAVFPIGDTPKPAIREEAARRGLAVADKADSHDICFIPSGDT
Mb3050c|M.bovis_AF2122/97 VLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSGNT
Rv3024c|M.tuberculosis_H37Rv VLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSGNT
MLBr_01707|M.leprae_Br4923 VLTAQQLRHAAFPIGDTLKRQVRAEAARRGLLVADKPDSHGICFIPSGNT
MAV_3871|M.avium_104 VLSAEQLRHAAFPIGDTPKPQIREEAARRGLAVAEKPDSHDICFIPSGNT
MMAR_1690|M.marinum_M VLTAEQLRHAMFPIGDTPKPQIREEASRRGLAVADKPDSHDICFIPSGNT
MUL_1928|M.ulcerans_Agy99 VLTAEQLRHAMFPIGDTPKPQIREEASRRGLAVADKPDSHDICFIPSGNT
TH_1186|M.thermoresistible__bu VLTAEQLRRAIFPIGDTPKQQIREEAARRGLAVADKPDSHDICFIPSGDT
MAB_3356c|M.abscessus_ATCC_199 VLTPEQLRRAMFPVGDSPKPDIRAEAEQRGLLVANKPDSHDICFIPSGDT
MSMEG_2358|M.smegmatis_MC2_155 VLTAEQLRHALFPIGDTPKPQIRAEAAERGLAVAEKPDSHDICFIPTGDT
**:.:***:* **:**: * :* ** . ** **:*.***.*****:*:*
Mflv_4254|M.gilvum_PYR-GCK KAFLGARIGVRRGNVVDAAGTVLAEHDGVHGFTIGQRKGLGIPGPGPDGS
Mvan_2106|M.vanbaalenii_PYR-1 RAFLGARIGVRRGSVVDATGAVLAEHDGVHGFTIGQRKGLGIPGPGPDGR
Mb3050c|M.bovis_AF2122/97 KAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGR
Rv3024c|M.tuberculosis_H37Rv KAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGR
MLBr_01707|M.leprae_Br4923 RAFLSECIGIRRGSVVDADGTVLAEHDGVHGFTVGQRKGLGIVGPGPNGR
MAV_3871|M.avium_104 RAFLGERIGVRRGAVVNADGTVLAEHDGVHGFTIGQRKGLGIAGPGPDGR
MMAR_1690|M.marinum_M QTFLGERIGVRRGTVVDAAGAVLATHDGVHGFTIGQRKGLGIPGPGPDGR
MUL_1928|M.ulcerans_Agy99 QTFLGERIGVRRGTVVDAAGAVLATHDGVHGFTIGQRKGLGIPGPGPDGR
TH_1186|M.thermoresistible__bu RAFLGARIGVRRGAVVDSSGTKLAEHDGVHGFTIGQRKGLGIAGPGPDGR
MAB_3356c|M.abscessus_ATCC_199 QAFLGARIGVRRGNVVDADGSVLATHAGVHEFTIGQRKGLGLVGPAADGR
MSMEG_2358|M.smegmatis_MC2_155 KAFLGARIGVRPGAVVDGSGTVLAEHDGVHGFTIGQRKGLGIAGPGADGR
::**. **:* * **:. * ** * *** **:***:***: **..:*
Mflv_4254|M.gilvum_PYR-GCK PRYVTGIDAQTGTVHVGDVADLEVTSLQGDAPVFTSGVAPTGPLECVVQV
Mvan_2106|M.vanbaalenii_PYR-1 PRYVTGIDAETGTVHVGDVTDLEVTSLLGEAPVFTSGVAPDAPVECAVQV
Mb3050c|M.bovis_AF2122/97 PRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV
Rv3024c|M.tuberculosis_H37Rv PRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQV
MLBr_01707|M.leprae_Br4923 PRYVTAIDADTATVYVGDAADLGVHTLIGRAPVFTAGAAPLGPVQCVVQI
MAV_3871|M.avium_104 PRYVTAIDADTATVRVGEAADLDVREMLGRAVVFTSGTAPSGPFECAVQV
MMAR_1690|M.marinum_M PRYVTAIDAETGTVRVGDVADLQVHALTGRAPIFTAGTAPTGPLECAVQV
MUL_1928|M.ulcerans_Agy99 PRYVTAIDAETGTVRVGDVADLQVHALTGRAPIFTAGTAPTGPLECAVQV
TH_1186|M.thermoresistible__bu PRYVTAIDPDSGTVRVGSAEELDVWRLRGERPVFTSGQPFDGPVECVVQV
MAB_3356c|M.abscessus_ATCC_199 PRYVTSIDAETATVRVGTVEDLEIWEFGGEPVVWTSGRVPGQPIECQVQV
MSMEG_2358|M.smegmatis_MC2_155 PRYVTAIDAETGTVRVGPAEDLEVWELTGERPVFTSGVEPGGPIECQVQV
*****.**.::.** ** . :* : : * ::*:* *.:* **:
Mflv_4254|M.gilvum_PYR-GCK RAHGGVADAVAELRGDTLEVSLRTPLRGVAPGQTLVLYRRDPAGDEVLAS
Mvan_2106|M.vanbaalenii_PYR-1 RAHGGVVDAVAELRDGALEVSLRTPLRGVAPGQTLVLYRSDPDGDEVLGS
Mb3050c|M.bovis_AF2122/97 RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGS
Rv3024c|M.tuberculosis_H37Rv RAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGS
MLBr_01707|M.leprae_Br4923 RAHGETAVAVVELIGDELFVRLCEPLRGVARGQTLVLYRLDPDGDEVLGS
MAV_3871|M.avium_104 RAHGETADAVAELVGDELVVRLRAPLRGVAPGQTLALYRHDPDGDEVLGS
MMAR_1690|M.marinum_M RAHGETTSAVAELVGDELSVRLHSPLRGVARGQTLVLYRPDPDGDEVLGS
MUL_1928|M.ulcerans_Agy99 RAHGETTSAVAELVGDELSVRLHSPLRGVARGQTLVLYRPDPDGDEVLGS
TH_1186|M.thermoresistible__bu RAHGGLAEAVAEVRGDHLEVELREPLRGVAPGQTMVLYRPDPAGDEVLGS
MAB_3356c|M.abscessus_ATCC_199 RAHGSVVDAIVEPGAERIHVRLRTALRGVAPGQTVVLYRPDAEGDEVLGS
MSMEG_2358|M.smegmatis_MC2_155 RAHGGITDTVAELRDGKLELSLRAPLRGVAPGQTMVLYRPDPDGDEVIAS
**** . ::.* : : * .***** ***:.*** *. ****:.*
Mflv_4254|M.gilvum_PYR-GCK ATIRATH----------
Mvan_2106|M.vanbaalenii_PYR-1 ATISAAR----------
Mb3050c|M.bovis_AF2122/97 ATIAGASGLSTGGNPGA
Rv3024c|M.tuberculosis_H37Rv ATIAGASGLSTGGNPGA
MLBr_01707|M.leprae_Br4923 ATIASMSA---------
MAV_3871|M.avium_104 ATIAGTSR---------
MMAR_1690|M.marinum_M ATITATSACSPVS----
MUL_1928|M.ulcerans_Agy99 ATITATSACSPVS----
TH_1186|M.thermoresistible__bu ATLTR------------
MAB_3356c|M.abscessus_ATCC_199 AVITRD-----------
MSMEG_2358|M.smegmatis_MC2_155 ATITRR-----------
*.: