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SDARVPRFPDALSTPSLNRGVGFTHEQRRALGLTGRLPAAVLTLDQQSERVWHQLQSLATDLGRNLLLEQ LHYRHELLYYKVLTEHLVELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIAAAFETFGLGPDDV DLIVCTDAEAILGIGDWGIGGIQIAVGKLALYTAGGGIDPRRSLAVSLDVGTDNEQLLQDPFYLGNRHAR RRGEEYDQFIKRYIEIAHRLFPRAMLHFEDFGPTNARMILRTYGADYCVFNDDVQGTGAVVVAAIDGGSH VTGVPVRDNTVIVFGAGTAGVGVADQIRDAMVADGATVEQATSQIWAVDKQGLLFDDMADLREFQVPYAK NRARLGVGASDRVGLVDAIKLAAPTVLLGSSTVSGAFNQEVVEAMMQSCDRPMIFPLSNPTSRMEAMPAD VLAWSGGKALVTTGTPVAPVEYHDIAYTIGQANNVLVFPGIGLGVIVAGARLLTQGMLRAAAKALAHQAS PTTTGDSLLPDVQDLRGISRTVAAAVYQAAVADNVATRIHADVGQAIRETMWLPEYD*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. ulcerans Agy99 | MUL_1467 | mez | - | 100% (548) | malate dehydrogenase |
| M. ulcerans Agy99 | MUL_4503 | mez_1 | 4e-21 | 28.61% (388) | [NAD] dependent malate oxidoreductase Mez_1 [Mycobacterium |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2360 | mez | 0.0 | 80.66% (548) | malate dehydrogenase |
| M. gilvum PYR-GCK | Mflv_2214 | - | 3e-21 | 29.13% (381) | malate dehydrogenase |
| M. tuberculosis H37Rv | Rv2332 | mez | 0.0 | 80.47% (548) | malate dehydrogenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_1384 | - | 7e-21 | 28.35% (381) | malate dehydrogenase |
| M. marinum M | MMAR_3643 | mez | 0.0 | 99.82% (548) | [NAD] dependent malate oxidoreductase Mez [Mycobacterium |
| M. avium 104 | MAV_1381 | - | 1e-21 | 28.65% (377) | NAD-dependent malic enzyme |
| M. smegmatis MC2 155 | MSMEG_5055 | - | 5e-22 | 28.87% (381) | NAD-dependent malic enzyme |
| M. thermoresistible (build 8) | TH_2059 | - | 6e-21 | 29.77% (346) | NAD-dependent malic enzyme |
| M. vanbaalenii PYR-1 | Mvan_4481 | - | 5e-21 | 27.82% (381) | malate dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_5055|M.smegmatis_MC2_155 ------------------------------------------MVIEDSEI
TH_2059|M.thermoresistible__bu -------------------------------------VRTPQVVVDDAEI
Mflv_2214|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4481|M.vanbaalenii_PYR-1 -------------------------MGSVTLTPVVETVRTPQVVIVDAEI
MAB_1384|M.abscessus_ATCC_1997 -------------------------------------MTASDVVVTDEEI
MAV_1381|M.avium_104 ----------------------------MSELLESIQSRAEQGAITDAEI
MUL_1467|M.ulcerans_Agy99 MSDARVPRFPDALSTPSLNRGVGFTHEQRRALGLTGRLPAAVLTLDQQSE
MMAR_3643|M.marinum_M MSDARVPRFPDALSTPSLNRGVGFTHEQRRALGLTGRLPAAVLTLDQQSE
Mb2360|M.bovis_AF2122/97 MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAE
Rv2332|M.tuberculosis_H37Rv MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAE
MSMEG_5055|M.smegmatis_MC2_155 FAAHEGGKLSVELKSPLDTQR-------------------ALSIAYTPGV
TH_2059|M.thermoresistible__bu FAHHEGGKLSVALKEPLDTQR-------------------ALSIAYTPGV
Mflv_2214|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4481|M.vanbaalenii_PYR-1 FEAHEGGKLSVALNSPLDTQR-------------------ALSIAYTPGV
MAB_1384|M.abscessus_ATCC_1997 FEAHIDGKLSVELKSPLDTQR-------------------ALSIAYTPGV
MAV_1381|M.avium_104 FAAHVGGKLSVSLTSPLDTQR-------------------ALSIAYTPGV
MUL_1467|M.ulcerans_Agy99 RVWHQLQSLATDLGRNLLLEQLHYRHELLYYKVLTEHLVELMPVVYTPTV
MMAR_3643|M.marinum_M RVWHQLQSLATDLGRNLLLEQLHYRHELLYYKVLTEHLVELMPVVYTPTV
Mb2360|M.bovis_AF2122/97 RVWHQLQSLATDLGRNLLLEQLHYRHEVLYFKVLADHLPELMPVVYTPTV
Rv2332|M.tuberculosis_H37Rv RVWHQLQSLATELGRNLLLEQLHYRHEVLYFKVLADHLPELMPVVYTPTV
MSMEG_5055|M.smegmatis_MC2_155 AQVSRAIAADATLAKKYTWANR-------------------LVAVVSDGS
TH_2059|M.thermoresistible__bu AQVSRAIATDRTLAAKYTWSNR-------------------LVAVVSDGS
Mflv_2214|M.gilvum_PYR-GCK --------------------------------------------MVSDGS
Mvan_4481|M.vanbaalenii_PYR-1 AQVSRAIAADKTLAARYTWAHR-------------------LVAVVSDGS
MAB_1384|M.abscessus_ATCC_1997 AQVSRAIAADASLAKKYTWAHR-------------------LVAVVSDGS
MAV_1381|M.avium_104 AQVSRAIAADHRQAAHYTWANR-------------------LVAVVSDGS
MUL_1467|M.ulcerans_Agy99 GEAIQRFSDEYRGQRGLFLSIDEPDEIAAAFETFGLGPDDVDLIVCTDAE
MMAR_3643|M.marinum_M GEAIQRFSDEYRGQRGLFLSIDEPDEIAAAFETFGLGPDDVDLIVCTDAE
Mb2360|M.bovis_AF2122/97 GEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAE
Rv2332|M.tuberculosis_H37Rv GEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAE
: :*..
MSMEG_5055|M.smegmatis_MC2_155 AVLGLGDIGAAASLPVMEGKAALFKTFGGLD---SIPLVLDT-NDPDEIV
TH_2059|M.thermoresistible__bu AVLGLGDIGPEASLPVMEGKCALFKAFAGLD---SIPIVLDT-KDPDEIV
Mflv_2214|M.gilvum_PYR-GCK AVLGLGDIGAAASLPVMEGKSELFKTFGGLD---SIPIVLDT-KDPDEIV
Mvan_4481|M.vanbaalenii_PYR-1 AVLGLGDIGAAASLPVMEGKSALFKTFGGLD---SIPIVLDT-NDPDEIV
MAB_1384|M.abscessus_ATCC_1997 AVLGLGDIGPSASLPVMEGKSALFKTFAGLN---SIPIVLDT-KDPDEIV
MAV_1381|M.avium_104 AVLGLGDIGPAASLPVMEGKCALFQAYGGLN---AIPIVLDT-KDPDEIV
MUL_1467|M.ulcerans_Agy99 AILGIGDWGIGG-IQIAVGKLALYTAGGGIDPRRSLAVSLDVGTDNEQLL
MMAR_3643|M.marinum_M AILGIGDWGVGG-IQIAVGKLALYTAGGGIDPRRSLAVSLDVGTDNEQLL
Mb2360|M.bovis_AF2122/97 AILGIGDWGVGG-IQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLL
Rv2332|M.tuberculosis_H37Rv AILGIGDWGVGG-IQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLL
*:**:** * . : : ** *: : .*:: .:.: **. .* ::::
MSMEG_5055|M.smegmatis_MC2_155 ETLVRLRPTFGAVN--LEDISAPRCFEIERRVIE----------------
TH_2059|M.thermoresistible__bu ETLIRLRPTFGAVN--LEDISAPRCFEIERRVVE----------------
Mflv_2214|M.gilvum_PYR-GCK ETLIRLRPTFGAVN--LEDISAPRCFEIERRVIE----------------
Mvan_4481|M.vanbaalenii_PYR-1 ETLIRLRPTFGAVN--LEDISAPRCFEIERRVIE----------------
MAB_1384|M.abscessus_ATCC_1997 ETLIRLRPTFGAVN--LEDISAPRCFEIERRVIE----------------
MAV_1381|M.avium_104 ETLVRLRPTFGAVN--LEDISAPRCFEIERRLIE----------------
MUL_1467|M.ulcerans_Agy99 QDPFYLGNRHARRRGEEYDQFIKRYIEIAHRLFPRAMLHFEDFGPTNARM
MMAR_3643|M.marinum_M QDPFYLGNRHARRRGEEYDQFIKRYIEIAHRLFPRAMLHFEDFGPTNARM
Mb2360|M.bovis_AF2122/97 ADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFEDFGPANARK
Rv2332|M.tuberculosis_H37Rv ADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFEDFGPANARK
. * .. . * * :* :*:.
MSMEG_5055|M.smegmatis_MC2_155 -----ALDCPVMHDDQHGTAIVVLAALLGAVKVLDRDLASLKVVVSGAGA
TH_2059|M.thermoresistible__bu -----ALDCPVMHDDQHGTAIVVLAALIGAAEVLDRDIYSLRVVISGAGA
Mflv_2214|M.gilvum_PYR-GCK -----ALDCPVMHDDQHGTAIVVLAALHGAAKVLERDMHALRVVISGAGA
Mvan_4481|M.vanbaalenii_PYR-1 -----ALDCPVMHDDQHGTAIVVLAALHGAAKALGRDMHSLRVVISGAGA
MAB_1384|M.abscessus_ATCC_1997 -----ALDCPVMHDDQHGTAIVVLAALMGASRVLGRDQQSLKVVISGAGA
MAV_1381|M.avium_104 -----ALDCPVMHDDQHGTAIVVLAALMGAGKLLGRDMSSLKVVVSGAGA
MUL_1467|M.ulcerans_Agy99 ILRTYGADYCVFNDDVQGTGAVVVAAIDGGSHVTGVPVRDNTVIVFGAGT
MMAR_3643|M.marinum_M ILRTYGADYCVFNDDVQGTGAVVVAAIDGGSHVTGVPVRDNTVIVFGAGT
Mb2360|M.bovis_AF2122/97 ILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGT
Rv2332|M.tuberculosis_H37Rv ILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGT
. * *::** :**. **:**: .. . ::: ***:
MSMEG_5055|M.smegmatis_MC2_155 AGVACTNILRNSGITD----------IVVLDSKGIVSGGRTDLNSFKAEL
TH_2059|M.thermoresistible__bu AGVACANILQAKGIRD----------IVVLDSKGILHPGREDMNEVKTEL
Mflv_2214|M.gilvum_PYR-GCK AGVACANILLAKGITD----------ITVLDSKGIIHPDRGDLNSFKAEL
Mvan_4481|M.vanbaalenii_PYR-1 AGVACANILLAKGIPD----------VTVLDSKGIVHTGRSDLNPFKAEL
MAB_1384|M.abscessus_ATCC_1997 AGVACANILLADGIND----------VTVLDSKGIVHTSRDDLNEFKADL
MAV_1381|M.avium_104 AGVACTNLLMAMGVSD----------ITVLDSRGILHAGRDDMNDVKAAL
MUL_1467|M.ulcerans_Agy99 AGVGVADQIRDAMVADGATVEQATSQIWAVDKQGLLFDDMADLREFQVPY
MMAR_3643|M.marinum_M AGVGVADQIRDAMVADGATVEQATSQIWAVDKQGLLFDDMADLREFQVPY
Mb2360|M.bovis_AF2122/97 AGMGIADQIRDAMVADGATLEQAVSQIWPLDRPGLLFDDMDDLRDFQVPY
Rv2332|M.tuberculosis_H37Rv AGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPY
**:. :: : : * : :* *:: . *:. .:.
MSMEG_5055|M.smegmatis_MC2_155 AQRTNPRGLTGG------LAEALAGADVFLGVS--AGLVPEELIATMAPN
TH_2059|M.thermoresistible__bu AQRTNPRGLTAG------LAEALADADVFLGVS--AGLVPEELIASMAPK
Mflv_2214|M.gilvum_PYR-GCK AQRTNPAGRTGG------VAEALDGADMFLGLS--AGLVPEEIIATMAPG
Mvan_4481|M.vanbaalenii_PYR-1 AERTNPAGRTGG------IAEALDGADMFLGVS--AGLVPEEIIATMAPG
MAB_1384|M.abscessus_ATCC_1997 AARTNPRGLTGG------QAEALAGADVFLGVS--AGKVDESLIATMADD
MAV_1381|M.avium_104 ARRTNPAGLTGS------LAEAIRGADVFLGLS--GGVVPEEMIAAMAPG
MUL_1467|M.ulcerans_Agy99 AKNRARLGVGASDRVGLVDAIKLAAPTVLLGSSTVSGAFNQEVVEAMMQS
MMAR_3643|M.marinum_M AKNRARLGVGASDRVGLVDAIKLAAPTVLLGSSTVSGAFNQEVVEAMMQS
Mb2360|M.bovis_AF2122/97 AKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTAS
Rv2332|M.tuberculosis_H37Rv AKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTAS
* . * . * : . ::** * * . :.:: :*
MSMEG_5055|M.smegmatis_MC2_155 G---IVFALSNPDPEIHPDAARKYAA-----VVATGRSDFP---------
TH_2059|M.thermoresistible__bu C---IVFALSNPDPEIHPDVARKYAA-----VVATGRSDFP---------
Mflv_2214|M.gilvum_PYR-GCK G---IVFALSNPDPEIHPDAARKYAS-----VVATGRSDFP---------
Mvan_4481|M.vanbaalenii_PYR-1 G---IVFALSNPDPEIHPDAARKYAA-----VVATGRSDFP---------
MAB_1384|M.abscessus_ATCC_1997 S---IVFACSNPDPEIHPHVAAKYAA-----IVATGRSDFP---------
MAV_1381|M.avium_104 G---IVFALSNPDPEIHPQIAAKYAT-----VVATGRSDFP---------
MUL_1467|M.ulcerans_Agy99 CDRPMIFPLSNPTSRMEAMPADVLAWSGGKALVTTGTPVAPVEYHDIAYT
MMAR_3643|M.marinum_M CDRPMIFPLSNPTSRMEAMPADVLAWSGGKALVTTGTPVAPVEYHDIAYT
Mb2360|M.bovis_AF2122/97 CKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYV
Rv2332|M.tuberculosis_H37Rv CKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYV
::*. *** ..:.. * * :::** . *
MSMEG_5055|M.smegmatis_MC2_155 -NQINNVLAFPGVFRGALDAGARRITEEMKVAAAHAIFSVVGDDLGVD--
TH_2059|M.thermoresistible__bu -NQINNVLAFPGVFRGALDAGARRITEEMKVAAARAIFSVIGDDLGVDXX
Mflv_2214|M.gilvum_PYR-GCK -NQINNVLAFPGVFRGALDAGARRITEKMKVAAAEAIFSVVGDDLSVD--
Mvan_4481|M.vanbaalenii_PYR-1 -NQINNVLAFPGVFRGALDAGARRITEKMKVAAAEAIFSVVGDDLAVD--
MAB_1384|M.abscessus_ATCC_1997 -NQINNVLAFPGVFRGALDAEATKITERMKVAAAEAIFSVVEPELAPN--
MAV_1381|M.avium_104 -NQINNVLAFPGVFRGALEAGARRITEKMMVAAAEAIFSVVSDDLAPD--
MUL_1467|M.ulcerans_Agy99 IGQANNVLVFPGIGLGVIVAGARLLTQGMLRAAAKALAHQASPTTTGD--
MMAR_3643|M.marinum_M IGQANNVLVFPGIGLGVIVAGARLLTQGMLRAAAKALAHQASPTTTGD--
Mb2360|M.bovis_AF2122/97 IGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGD--
Rv2332|M.tuberculosis_H37Rv IGQANNVLAFPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGD--
.* ****.***: *.: * * :*. * ***.*: :
MSMEG_5055|M.smegmatis_MC2_155 --------------------------------------------------
TH_2059|M.thermoresistible__bu XASPVFGPTPVTPIRVRNRSRASVSGKPYNVIESSRTIIAVINRASPPRR
Mflv_2214|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4481|M.vanbaalenii_PYR-1 --------------------------------------------------
MAB_1384|M.abscessus_ATCC_1997 --------------------------------------------------
MAV_1381|M.avium_104 --------------------------------------------------
MUL_1467|M.ulcerans_Agy99 --------------------------------------------------
MMAR_3643|M.marinum_M --------------------------------------------------
Mb2360|M.bovis_AF2122/97 --------------------------------------------------
Rv2332|M.tuberculosis_H37Rv --------------------------------------------------
MSMEG_5055|M.smegmatis_MC2_155 ---------------------------------------HIVPSALDPRV
TH_2059|M.thermoresistible__bu SVDRVAGVAITLYPTPAASITAWSSAISSTSPRTEAITGHPATSAAAPPS
Mflv_2214|M.gilvum_PYR-GCK ---------------------------------------HIVPSALDPRV
Mvan_4481|M.vanbaalenii_PYR-1 ---------------------------------------HIVPSALDPRV
MAB_1384|M.abscessus_ATCC_1997 ---------------------------------------KIVPSPLDPRV
MAV_1381|M.avium_104 ---------------------------------------RIVPSPLDTRV
MUL_1467|M.ulcerans_Agy99 ---------------------------------------SLLPDVQDLRG
MMAR_3643|M.marinum_M ---------------------------------------SLLPDVQDLRG
Mb2360|M.bovis_AF2122/97 ---------------------------------------SLLPDVQNLRA
Rv2332|M.tuberculosis_H37Rv ---------------------------------------SLLPDVQNLRA
..
MSMEG_5055|M.smegmatis_MC2_155 G----------------PAVAAAVAEASGV--------------------
TH_2059|M.thermoresistible__bu GGRGTCVAVGRPGPRPWPSARASAPASSGRSPTPTRPRHQPVSVLSVAQG
Mflv_2214|M.gilvum_PYR-GCK G----------------PAVAAAVAAASED--------------------
Mvan_4481|M.vanbaalenii_PYR-1 G----------------PAVAAAVAAASEE--------------------
MAB_1384|M.abscessus_ATCC_1997 G----------------PAVAAAVQAVAHESD------------------
MAV_1381|M.avium_104 G----------------DAVATAVALATEVSDAPAG--------------
MUL_1467|M.ulcerans_Agy99 IS---------------RTVAAAVYQAAVADNVATRIHADVGQAIRETMW
MMAR_3643|M.marinum_M IS---------------RTVAAAVYQAAVADNVATRIHADVGQAIRETMW
Mb2360|M.bovis_AF2122/97 IS---------------TTVAEAVYRAAVQDGVASRTHDDVRQAIVDTMW
Rv2332|M.tuberculosis_H37Rv IS---------------TTVAEAVYRAAVQDGVASRTHDDVRQAIVDTMW
:. :. :
MSMEG_5055|M.smegmatis_MC2_155 ------
TH_2059|M.thermoresistible__bu FSSPSR
Mflv_2214|M.gilvum_PYR-GCK ------
Mvan_4481|M.vanbaalenii_PYR-1 ------
MAB_1384|M.abscessus_ATCC_1997 ------
MAV_1381|M.avium_104 ------
MUL_1467|M.ulcerans_Agy99 LPEYD-
MMAR_3643|M.marinum_M LPEYD-
Mb2360|M.bovis_AF2122/97 LPAYD-
Rv2332|M.tuberculosis_H37Rv LPAYD-