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VIPRSPEQMPLWDEFGQQPLFAQSPLLLGERAAPWWVILYRTGQTLAPQPSRGELHEFKVVPVARSNLIE RQASCWGYVYSSAGWEPPNRGVVDVAGFAVLDFETTGFAAEYDRVVEVGLVLLSAEGDIEDEWSTLINPH RDVGATERHGIRARDVLGAPAFEAIIPRLLRDLRGRVFVAHNVNFDQRFLLAELRRAGVPLDNPWLTGLC TMRWAGRLLPAASRKLPDCCDAAGVFLTDAHRALADARATASLLKYLIDHCGSPPPWAKEIDGAEDFEWP QVAGPEVRLVTRAAWGAAPPRPPAGWLDRIVARMPHEDDPRVESYLEILESALLDCYLSCHEEDALVEMA DSLGLSRRLLDEIHHDYLRSMARVAVADGVVTDDERADLLQVAELLGLSADRVDAALAMASARSACAEFA LAAGDVLCLTGSMRRRRSEWEAELAQRGIEVGSLTRRTRLLVAADPDSATGKARKARKYGIPIINEDALS RLLGARTSGE
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. ulcerans Agy99 | MUL_1148 | - | - | 100% (500) | DNA polymerase |
| M. ulcerans Agy99 | MUL_4303 | dnaQ | 6e-18 | 35.63% (174) | DNA polymerase III subunit epsilon |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2214 | - | 6e-18 | 34.38% (192) | hypothetical protein Mb2214 |
| M. gilvum PYR-GCK | Mflv_1292 | - | 1e-21 | 37.71% (175) | DNA polymerase III subunit epsilon |
| M. tuberculosis H37Rv | Rv2191 | - | 6e-18 | 34.38% (192) | hypothetical protein Rv2191 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0329 | - | 4e-21 | 34.11% (214) | DNA polymerase III subunit epsilon |
| M. marinum M | MMAR_5228 | dnaQ | 1e-17 | 35.63% (174) | DNA polymerase III (epsilon subunit) DnaQ |
| M. avium 104 | MAV_0391 | - | 6e-19 | 34.69% (196) | DNA polymerase III subunit epsilon |
| M. smegmatis MC2 155 | MSMEG_6275 | - | 2e-22 | 39.13% (161) | DNA polymerase III subunit epsilon |
| M. thermoresistible (build 8) | TH_2284 | dnaQ | 1e-18 | 38.06% (155) | PROBABLE DNA POLYMERASE III (EPSILON SUBUNIT) DNAQ |
| M. vanbaalenii PYR-1 | Mvan_5519 | - | 9e-22 | 37.71% (175) | DNA polymerase III subunit epsilon |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_6275|M.smegmatis_MC2_155 --------------------------------------------------
TH_2284|M.thermoresistible__bu --------------------------------------------------
Mflv_1292|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_5519|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5228|M.marinum_M --------------------------------------------------
MAV_0391|M.avium_104 --------------------------------------------------
MAB_0329|M.abscessus_ATCC_1997 --------------------------------------------------
Mb2214|M.bovis_AF2122/97 --------------------------------------------------
Rv2191|M.tuberculosis_H37Rv --------------------------------------------------
MUL_1148|M.ulcerans_Agy99 MIPRSPEQMPLWDEFGQQPLFAQSPLLLGERAAPWWVILYRTGQTLAPQP
MSMEG_6275|M.smegmatis_MC2_155 ------------------------MSRAPLSSTPQLWGRPADEPGSGWAV
TH_2284|M.thermoresistible__bu --------------------------------------------------
Mflv_1292|M.gilvum_PYR-GCK ----------------MRHVRAPCHPRIMSSTAGRRWGRPAHEDGAGWAV
Mvan_5519|M.vanbaalenii_PYR-1 ----------------------------MSSTAGSRWGRPADQPGAGWAV
MMAR_5228|M.marinum_M -------------------------------MSQN-WGRPASHPGEGWAV
MAV_0391|M.avium_104 -------------------------------MSAVCWGRPASEPDAGWAV
MAB_0329|M.abscessus_ATCC_1997 ---------------------------------MPIWGQTPDHS-PKWVV
Mb2214|M.bovis_AF2122/97 -----MQGPNVAAMGATGGTQLSFADLAHAQGAAWTPADEMSLRETTFVV
Rv2191|M.tuberculosis_H37Rv -----MQGPNVAAMGATGGTQLSFADLAHAQGAAWTPADEMSLRETTFVV
MUL_1148|M.ulcerans_Agy99 SRGELHEFKVVPVARSNLIERQASCWGYVYSSAGWEPPNRGVVDVAGFAV
MSMEG_6275|M.smegmatis_MC2_155 VDVETTGFRPG-----QARIVSVAALAVGDDGSVEHSMSSLLNPG--VDP
TH_2284|M.thermoresistible__bu --VETTGFRPG-----QARIVSIAALAVGDDGNVERSVATLLNPG--VDP
Mflv_1292|M.gilvum_PYR-GCK VDVETSGFRPG-----QARIVSVAALALSDDGNVEKSLYSLLDPG--VDP
Mvan_5519|M.vanbaalenii_PYR-1 VDVETSGFRPG-----QARIVSVAALALSDDGNVEKSLYSLLDPG--VDP
MMAR_5228|M.marinum_M VDVETSGFRPG-----HARVISVAALGLDAAGNVEHSVVSLLNPG--VDP
MAV_0391|M.avium_104 IDVETSGFRPG-----QARIISLAVLGLDAGGKVEQSVVSLLNPG--VDP
MAB_0329|M.abscessus_ATCC_1997 VDLETSGFQPG-----RARVISVAALAMDADGTITDSVVSLLNPG--VDP
Mb2214|M.bovis_AF2122/97 VDLETTGGRTTGNDATPPDAITEIGAVKVCGGAVLGEFATLVNPQHSIPP
Rv2191|M.tuberculosis_H37Rv VDLETTGGRTTGNDATPPDAITEIGAVKVCGGAVLGEFATLVNPQHSIPP
MUL_1148|M.ulcerans_Agy99 LDFETTGFAAEY-----DRVVEVGLVLLSAEGDIEDEWSTLINPHR--DV
.**:* . : * : . :*::*
MSMEG_6275|M.smegmatis_MC2_155 GPTHVHGLTAEMLEGQPCFADVVDELIEVLRGRTLVAHNAGFDYSFLAAE
TH_2284|M.thermoresistible__bu GPTHIHGLTADMLAGQPQFADIVDDLIDILRGRTLVAHNVAFDYAFLVAE
Mflv_1292|M.gilvum_PYR-GCK GPTHVHGLTAEMLEGQPTFGDVVGDLCEVLRGRTLVAHNVGFDYSFLSAE
Mvan_5519|M.vanbaalenii_PYR-1 GPTHVHGLTAEMLAGQPAFGDVVGDLIEVLRGRTLVAHNVGFDYSFLTAE
MMAR_5228|M.marinum_M GPTHVHGLTAAMLEDQPQFADIVGDLTELLRGRTLVAHNVAFDYSFLAAE
MAV_0391|M.avium_104 GPTHVHGLTAAMLEDQPQFADIAGDVVEVLRGRTLVAHNVAFDYAFLAAE
MAB_0329|M.abscessus_ATCC_1997 GPTHVHGLTPEMLEGQPEFSDIAPDLIALLRGRTLVAHNVGFDYSFLAAE
Mb2214|M.bovis_AF2122/97 QIVRLTGITTAMVGNAPTIDAVLPMFFEFAGDSVLVAHNAGFDIGFLRAA
Rv2191|M.tuberculosis_H37Rv QIVRLTGITTAMVGNAPTIDAVLPMFFEFAGDSVLVAHNAGFDIGFLRAA
MUL_1148|M.ulcerans_Agy99 GATERHGIRARDVLGAPAFEAIIPRLLRDLRGRVFVAHNVNFDQRFLLAE
.. *: . : . * : : . . .:****. ** ** *
MSMEG_6275|M.smegmatis_MC2_155 AELVGAELP--IDTAMCTVELARRLELG--TENLRLETLAAHWGVTQMKP
TH_2284|M.thermoresistible__bu AELIDAELP--IDSVMCTIELARRMQLG--TENLRLETLAAHWGVTQLRA
Mflv_1292|M.gilvum_PYR-GCK AELVGAELP--IDTVMCTVELARRLDLG--LENLRLETLAAHWGVTQMRP
Mvan_5519|M.vanbaalenii_PYR-1 AEMVHAELP--VDTVMCTVELARRLDLG--LDNLRLETLAAHWGVTQMKP
MMAR_5228|M.marinum_M AELADAELP--VDSVMCTVELARRLELG--IDNLRLETLAAHWGVTQERP
MAV_0391|M.avium_104 AELAGIELP--VDTVMCTVELARRLDLG--IDNLRLETLAAYWGVTQQRP
MAB_0329|M.abscessus_ATCC_1997 AELVGAELP--TEAVMCTVELTRRLELD--TPNLRLETLAAHWGVEQLKA
Mb2214|M.bovis_AF2122/97 ARRCDITWP--QPQVLCTMRLARRVLSRDEAPSVRLAALARLFAVASNPT
Rv2191|M.tuberculosis_H37Rv ARRCDITWP--QPQVLCTMRLARRVLSRDEAPSVRLAALARLFAVASNPT
MUL_1148|M.ulcerans_Agy99 LRRAGVPLDNPWLTGLCTMRWAGRLLPA---ASRKLPDCCDAAGVFLTDA
. :**:. : *: . :* . .* .
MSMEG_6275|M.smegmatis_MC2_155 HDALDDALVLAQILKPTLARARDR----------KVWLP-------LRQL
TH_2284|M.thermoresistible__bu HDALDDALVLTQILKPTLALARER----------RTWLP-------LRPV
Mflv_1292|M.gilvum_PYR-GCK HDALDDAMVLAQILKPVLVRAQER----------RKWLP-------VHPV
Mvan_5519|M.vanbaalenii_PYR-1 HDALDDAMVLAQILKPVLVRAQER----------RKWLP-------VHPV
MMAR_5228|M.marinum_M HDAFDDALVLSGILAPALQRARER----------DVWLP-------IHPV
MAV_0391|M.avium_104 HDAFDDAMVLTGVLASALQRARER----------DIWLP-------VHPV
MAB_0329|M.abscessus_ATCC_1997 HDAYDDARVLAEVLPKVLERARER----------GTWLP-------IRET
Mb2214|M.bovis_AF2122/97 HRALDDARATVDVLHALIERVGNQGVHTYAEL--RSYLPNVTQAQRCKRV
Rv2191|M.tuberculosis_H37Rv HRALDDARATVDVLHALIERVGNQGVHTYAEL--RSYLPNVTQAQRCKRV
MUL_1148|M.ulcerans_Agy99 HRALADARATASLLKYLIDHCGSPPPWAKEIDGAEDFEWPQVAGPEVRLV
* * ** . :* : . : :
MSMEG_6275|M.smegmatis_MC2_155 TRKRWPN-------------------------------------------
TH_2284|M.thermoresistible__bu TRRHWPN-------------------------------------------
Mflv_1292|M.gilvum_PYR-GCK GRRTWPN-------------------------------------------
Mvan_5519|M.vanbaalenii_PYR-1 SRRTWPN-------------------------------------------
MMAR_5228|M.marinum_M TRRRWPN-------------------------------------------
MAV_0391|M.avium_104 TRRRWPN-------------------------------------------
MAB_0329|M.abscessus_ATCC_1997 TRKRWPN-------------------------------------------
Mb2214|M.bovis_AF2122/97 LAETLPHRPGVYLFRGPSGEVLYVGTAADLRRRVSQYFNGTDRRKRMTEM
Rv2191|M.tuberculosis_H37Rv LAETLPHRPGVYLFRGPSGEVLYVGTAADLRRRVSQYFNGTDRRKRMTEM
MUL_1148|M.ulcerans_Agy99 TRAAWGAAPPRPPAG-----------------------------------
MSMEG_6275|M.smegmatis_MC2_155 ------------GHVTHDELRPLKVEASRLP-------------------
TH_2284|M.thermoresistible__bu ------------GRTTHEQLRPLKALASRIP-------------------
Mflv_1292|M.gilvum_PYR-GCK ------------GRVTHDEFRPLKALAARMA-------------------
Mvan_5519|M.vanbaalenii_PYR-1 ------------GRITHDEFRPLKALAARMP-------------------
MMAR_5228|M.marinum_M ------------GRVTHDELRPLKALASRMP-------------------
MAV_0391|M.avium_104 ------------GRVTHDELRPLKVLASRMP-------------------
MAB_0329|M.abscessus_ATCC_1997 ------------GRVTHDELRPLKTLASRQP-------------------
Mb2214|M.bovis_AF2122/97 VMLASSIDHVECAHPLEAGVRELRMLSTHAPPYNRRSKFPYRWWWVALTD
Rv2191|M.tuberculosis_H37Rv VMLASSIDHVECAHPLEAGVRELRMLSTHAPPYNRRSKFPYRWWWVALTD
MUL_1148|M.ulcerans_Agy99 ------WLDRIVARMPHEDDPRVESYLEILES------------------
.: . :.
MSMEG_6275|M.smegmatis_MC2_155 --------------------------------------------------
TH_2284|M.thermoresistible__bu --------------------------------------------------
Mflv_1292|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_5519|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5228|M.marinum_M --------------------------------------------------
MAV_0391|M.avium_104 --------------------------------------------------
MAB_0329|M.abscessus_ATCC_1997 --------------------------------------------------
Mb2214|M.bovis_AF2122/97 EAFPRLSVIRAPRHDRVVGPFRSRSKAAETTALLARCTGLRTCTTRLTRS
Rv2191|M.tuberculosis_H37Rv EAFPRLSVIRAPRHDRVVGPFRSRSKAAETAALLARCTGLRTCTTRLTRS
MUL_1148|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_6275|M.smegmatis_MC2_155 ------CRYANPGPFVAGR---------------PLVQGMRVALSAEVVR
TH_2284|M.thermoresistible__bu ------CRYTNPGRYIAGR---------------PLVQGMQVALSAEVTH
Mflv_1292|M.gilvum_PYR-GCK ------CPYANPGRFVSGR---------------PLVQGMRVALAAEVGH
Mvan_5519|M.vanbaalenii_PYR-1 ------CAYANPGVFVAGR---------------PLVQGMRVALSAEVGH
MMAR_5228|M.marinum_M ------CPYLNPGRYVCDR---------------PLVQGMRVALAAEVGR
MAV_0391|M.avium_104 ------CPYLNPGPYVGGR---------------PLVQGMRVALAAEVER
MAB_0329|M.abscessus_ATCC_1997 ------CHWLNPGPYTAGQ---------------PLVQGMRVALSSEVSR
Mb2214|M.bovis_AF2122/97 ARHGPACPELEVSACPAARDVTAAQYAEAVLRAAALIGGLDNAALAAAVQ
Rv2191|M.tuberculosis_H37Rv ARHGPACPELEVSACPAARDVTAAQYAEAVLRAAALIGGLDNAALAAAVQ
MUL_1148|M.ulcerans_Agy99 ALLDCYLSCHEEDALVEMADSLGLSRRLLDEIHHDYLRSMARVAVADGVV
: . : .: . :
MSMEG_6275|M.smegmatis_MC2_155 THEELLERVLHAGLAYS-----------------DMVDLQTSLVICNDPA
TH_2284|M.thermoresistible__bu THEELIERILHAGLAYT-----------------DDITGQTSLVVCNEPN
Mflv_1292|M.gilvum_PYR-GCK TYDELIERIVHAGLAYT-----------------ESVDAFTSLVVCDAAA
Mvan_5519|M.vanbaalenii_PYR-1 THEELVERILHAGLAFT-----------------EVVDGHTSLVVCDDPA
MMAR_5228|M.marinum_M THEELVERILHAGLAYS-----------------DGVDRETSLVVCNEDA
MAV_0391|M.avium_104 THEELVERILHAGLAYS-----------------DTVDRETSLVVCNDPA
MAB_0329|M.abscessus_ATCC_1997 THEELIEQIMQAGLAYA-----------------DAVDEDTSLVICNQEN
Mb2214|M.bovis_AF2122/97 QVTELAERRRYESAARLRDHLATAIEALWHGQRLRALAALPELIAAKPDG
Rv2191|M.tuberculosis_H37Rv QVTELAERRRYESAARLRDHLATAIEALWHGQRLRALAALPELIAAKPDG
MUL_1148|M.ulcerans_Agy99 TDDERADLLQVAELLGLS---------------ADRVDAALAMASARSAC
* : : : .
MSMEG_6275|M.smegmatis_MC2_155 PEQG-------------------KGYQAKELGVPLVRDDEFMRLLDHVVG
TH_2284|M.thermoresistible__bu PEHG-------------------KGFHAADLGVPLMSDTEFMAALDRVVG
Mflv_1292|M.gilvum_PYR-GCK PEQG-------------------KGYQAGEFGVPMLPSDEFLQRLDAVIG
Mvan_5519|M.vanbaalenii_PYR-1 PEQG-------------------KGYQAGGFGVPTLGSAEFLRHLDTVIG
MMAR_5228|M.marinum_M PDQG-------------------KGYHARQLGVPVLTDTQFMDRVGCVLG
MAV_0391|M.avium_104 AEHG-------------------KGYLARQLGVPVISDAQFLDCVRAVIG
MAB_0329|M.abscessus_ATCC_1997 PEQG-------------------KGFHARSLGVPLLDDRTFMTEVRRVVG
Mb2214|M.bovis_AF2122/97 PREGGYQLAVIRHGQLAAAGRAPRGVPPMPVVDAIRRGAQAILPTPAPLG
Rv2191|M.tuberculosis_H37Rv PREGGYQLAVIRHGQLAAAGRAPRGVPPMPVVDAIRRGAQAILPTPAPLG
MUL_1148|M.ulcerans_Agy99 AEFALAAGDVLCLTG----SMRRRRSEWEAELAQRGIEVGSLTRRTRLLV
. . : : :
MSMEG_6275|M.smegmatis_MC2_155 GADVETFVDTTADGDQYALF------------------------------
TH_2284|M.thermoresistible__bu GVEIDEFVDTTKAGEQYALF------------------------------
Mflv_1292|M.gilvum_PYR-GCK GSAVEEFAEPSPAGDQFALF------------------------------
Mvan_5519|M.vanbaalenii_PYR-1 GSAIEEFADQASAGDQFTLF------------------------------
MMAR_5228|M.marinum_M GTSAEKFTDHTLVEEQFALF------------------------------
MAV_0391|M.avium_104 GTSMDEFTDATP-DPQLALF------------------------------
MAB_0329|M.abscessus_ATCC_1997 GTDIEQFTVPSRAGAQYSLF------------------------------
Mb2214|M.bovis_AF2122/97 GALVEEIALIARWLAEPGVRIVGVSNDAAGLASPVRSAGPWAAWAATARS
Rv2191|M.tuberculosis_H37Rv GALVEEIALIARWLAEPGVRIVGVSNDAAGLASPVRSAGPWAAWAATARS
MUL_1148|M.ulcerans_Agy99 AADPDSATGKARKARKYGIPIINEDALSRLLGARTSGE------------
. : . : : :
MSMEG_6275|M.smegmatis_MC2_155 --------------------------------------------------
TH_2284|M.thermoresistible__bu --------------------------------------------------
Mflv_1292|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_5519|M.vanbaalenii_PYR-1 --------------------------------------------------
MMAR_5228|M.marinum_M --------------------------------------------------
MAV_0391|M.avium_104 --------------------------------------------------
MAB_0329|M.abscessus_ATCC_1997 --------------------------------------------------
Mb2214|M.bovis_AF2122/97 AQLAGEQLSRGWQSDLPTEPHPSREQLFGRTGVDCRTGPPQPLLPGRQPF
Rv2191|M.tuberculosis_H37Rv AQLAGEQLSRGWQSDLPTEPHPSREQLFGRTGVDCRTGPPQPLLPGRQPF
MUL_1148|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_6275|M.smegmatis_MC2_155 ----
TH_2284|M.thermoresistible__bu ----
Mflv_1292|M.gilvum_PYR-GCK ----
Mvan_5519|M.vanbaalenii_PYR-1 ----
MMAR_5228|M.marinum_M ----
MAV_0391|M.avium_104 ----
MAB_0329|M.abscessus_ATCC_1997 ----
Mb2214|M.bovis_AF2122/97 STAG
Rv2191|M.tuberculosis_H37Rv STAG
MUL_1148|M.ulcerans_Agy99 ----