For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. smegmatis MC2 155 MSMEG_5984 (-)

MTAQISNFDSGFGTRQLVSMPKPNSVVAGTNMSIKLPPSSVFQRSKWQRRYSSYLLMTDTLVIALSVGFA
QLVRFGPTLNPPGYSKLYVPAFSILFAIAWLVAMSALRTRSPRITAACVDEYRRVTAASFWTFGAIAMVS
LLLKLDIARGYLAVALPVGTLGLLLERRLWHRHVARERVAGRYRTAVLAFGEIGAVTELVSELVRNPADG
YDVVGVGVPRYGDARGEFVMVSGQRIPIIGGEAEMLDAVHTCGADTVAIAGTEAFGVRGIRRLLWQLEPL
GVELVVSTGAMDVALSRLVMQPIAGIPLLHIEKPLYRNAKRFQKCLFDLSFAFAVLLITSPLLLITAIAI
KATSRGPVFYSAERIGVDGKLFSMLKFRTMVENADQMLDELEDLNESDGLLFKIHDDPRVTRVGKVLRRL
SIDELPQFINVLRGEMSVVGPRPPLRREVEEYDCEILRRLLVKPGVTGLWQVSGRSDLSWDQAVRLDLSY
VDNWSMIGDILIVAKTFGAVLRKDGAY*

Operon Prediction Model: Genebank

Paralogs

species	id	gene	e-value	identity (len)	annotation
M. smegmatis MC2 155	MSMEG_5984	-	-	100% (518)	undecaprenyl-phosphate galactosephosphotransferase

Closest Orthologs (e-value cutoff: 1e-4)

species	id	gene	e-value	identity (len)	annotation
M. bovis AF2122 / 97	-	-	-	-	-
M. gilvum PYR-GCK	Mflv_4755	-	1e-124	50.11% (475)	undecaprenyl-phosphate galactose phosphotransferase
M. tuberculosis H37Rv	-	-	-	-	-
M. leprae Br4923	-	-	-	-	-
M. abscessus ATCC 19977	MAB_1020	-	3e-85	40.97% (432)	undecaprenyl-phosphate galactosephosphotransferase
M. marinum M	MMAR_4503	-	1e-160	57.93% (473)	hypothetical protein MMAR_4503
M. avium 104	-	-	-	-	-
M. thermoresistible (build 8)	-	-	-	-	-
M. ulcerans Agy99	MUL_4674	-	1e-155	55.60% (473)	hypothetical protein MUL_4674
M. vanbaalenii PYR-1	Mvan_1703	-	1e-127	51.48% (474)	undecaprenyl-phosphate galactose phosphotransferase

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_4755|M.gilvum_PYR-GCK          --MTAINDR---LDVAANIVSLPTK------------APT--------WQ
Mvan_1703|M.vanbaalenii_PYR-1       --MTAVNDR---IEVAANIVQIPAK------------APA--------WQ
MMAR_4503|M.marinum_M               --MTSLLHG-----RTGDCPATPDR----------VHSTDIGVCRR--WQ
MUL_4674|M.ulcerans_Agy99           --MTSLLHG-----RTGDCPATPDP----------MGSPTRDTVRR--WR
MSMEG_5984|M.smegmatis_MC2_155      -MMTAQISNFDSGFGTRQLVSMPKPNSVVAGTNMSIKLPPSSVFQRSKWQ
MAB_1020|M.abscessus_ATCC_1997      MVIKSVVND----EIQQRVEKRPGG-----------------------LF
                                      :.:                 *                           

Mflv_4755|M.gilvum_PYR-GCK          QGYSRRLIVLDLVGVVLAVGLAHWLRFGSETETISTYRAVDYTMVSVAIA
Mvan_1703|M.vanbaalenii_PYR-1       RGYARRLVAIDLLGVILAVGLAQWLRFGGLSGEVSTFRYVDYTMVSVGLA
MMAR_4503|M.marinum_M               LQYSRRLRVTDSAIVFAAVMLAQYVRFG-DSPNKSGYPGPVMTLFSFLFA
MUL_4674|M.ulcerans_Agy99           LQYSRRLRITDSAIVFAAVMLAQYVRFG-DSPNRSGYPGPVMTLFSVLFA
MSMEG_5984|M.smegmatis_MC2_155      RRYSSYLLMTDTLVIALSVGFAQLVRFG-PTLNPPGYSKLYVPAFSILFA
MAB_1020|M.abscessus_ATCC_1997      YLHRRWAPAADLVAIVLTSLLSYQLAHHLLSGRQD--LGGWYVATDAVIA
                                      :       *   :  :  ::  : .   :              .  :*

Mflv_4755|M.gilvum_PYR-GCK          VLWLAALAINHSRSTRVIGSGAEEYRRVILATLAVFGAVAIVSMLFKLEI
Mvan_1703|M.vanbaalenii_PYR-1       VLWMAALSINNSRSTRVIGSGAEEYRRVWLATLTVFGGVAILSMLFKLEI
MMAR_4503|M.marinum_M               ALWLSCLAIFNTRSQHVIGAGIDEYRRISSASFWTFGAIAMVTLLAKIDL
MUL_4674|M.ulcerans_Agy99           ALWLSCLAIFNTRSQHVIGVGIDEYRRISSASFWTFGAIAMVTLLAKIDL
MSMEG_5984|M.smegmatis_MC2_155      IAWLVAMSALRTRSPRITAACVDEYRRVTAASFWTFGAIAMVSLLLKLDI
MAB_1020|M.abscessus_ATCC_1997      VVWFAALSLHTVGPGKALRAVLQEWQTVVGVTFQLFTIVLLTYLISRIEL
                                      *: .::     . :      :*:: :  .::  *  : :  :: ::::

Mflv_4755|M.gilvum_PYR-GCK          ARG-FLVIALPVGVVVLVVLRWLARKVVVTVRRKYGRCITRVLVVGSPTA
Mvan_1703|M.vanbaalenii_PYR-1       ARG-YLMIALPAGLLFLGFFRWVARQMVARAREKHGRCITRVLVVGSPSA
MMAR_4503|M.marinum_M               ARG-YLAVALPVGTIGLLMSRNGWRRYVSRKR-AQGQCQTMALAIGDRPG
MUL_4674|M.ulcerans_Agy99           ARG-YLAVVLPVGTIALLASRNGWRKYVGRKR-ARGQCQTIALAIGDRQG
MSMEG_5984|M.smegmatis_MC2_155      ARG-YLAVALPVGTLGLLLERRLWHRHVARER-VAGRYRTAVLAFGEIGA
MAB_1020|M.abscessus_ATCC_1997      IDIRHLIIEIPLGLVGIFIARHVLRRVWGSEA------RTSVLLAGDHDA
                                        .* : :* * : :   *   ::             * .*  *.  .

Mflv_4755|M.gilvum_PYR-GCK          VRDLTMSLHREAASEYEVVGACTPG-ITPHSEIDVSGV-GAIPTFGDESN
Mvan_1703|M.vanbaalenii_PYR-1       VRDLTTSLAREPRSEYEVVGACIPG-HNPRTKIEVPGV-GAIPNFGDESH
MMAR_4503|M.marinum_M               VSRLIHELVRNPRDGYSVVGVCIPGSGAPRGDVLQVEG-RKVPILGDESH
MUL_4674|M.ulcerans_Agy99           VSRLIQELVRNPRDGYSVVGVCIPGYGSPRGDVLQVEG-RQVPILGDESH
MSMEG_5984|M.smegmatis_MC2_155      VTELVSELVRNPADGYDVVGVGVPRYGDARGEFVMVSG-QRIPIIGGEAE
MAB_1020|M.abscessus_ATCC_1997      VREMVTAFDRHRDSGFEVVGACTPATDTRYGATEIEVGDRRVPVVGDDRN
                                    *  :   : *.  . :.***.  *                 :* .*.: .

Mflv_4755|M.gilvum_PYR-GCK          VVGAVTATRSHAVAVTATERLGGRGIRDLSWELEKLDIDLLVAPGVVDIA
Mvan_1703|M.vanbaalenii_PYR-1       VVGAVTATNSHAVAVAATERLNGRGIRDLSWELEKLDIDLLVAPGVVDIA
MMAR_4503|M.marinum_M               AVAAIDECGADTVVVTQTEQFGVHAIRNLIWQLETLDVDLVVSPGVMDIA
MUL_4674|M.ulcerans_Agy99           AMAAIDECGADTVVVTQAEQFGVHAIRNLIWRLETLDVDLVVSPGVMDIA
MSMEG_5984|M.smegmatis_MC2_155      MLDAVHTCGADTVAIAGTEAFGVRGIRRLLWQLEPLGVELVVSTGAMDVA
MAB_1020|M.abscessus_ATCC_1997      VIEAAQRTSAQIVAVAMTNELGRKELVGLASDLGDLGIELAVSPGDVDET
                                     : *     :. *.:: :: :. : :  *   *  *.::* *:.* :* :

Mflv_4755|M.gilvum_PYR-GCK          GPRLQMRPVAGLPLIHVEKPQYHGAKKFQKRLFDVAFSSAVLLCGLPILL
Mvan_1703|M.vanbaalenii_PYR-1       GPRLQMRPVAGLPLIHVEKPQYHGAKRFQKRFFDIAFSSAVLIFGMPLLI
MMAR_4503|M.marinum_M               QARLTLRPVAGFPLLHVEKPQYSGAQRFQKRAFDFCFALTALLVASPVLI
MUL_4674|M.ulcerans_Agy99           QARLTLRPVAGFPLLHVEKPQYLGAQRFQKRAFDFCFALTALIAASPLLI
MSMEG_5984|M.smegmatis_MC2_155      LSRLVMQPIAGIPLLHIEKPLYRNAKRFQKCLFDLSFAFAVLLITSPLLL
MAB_1020|M.abscessus_ATCC_1997      AVTTADRGLERVHMLEMLAPGYRRARSMSKEAFDMVFATAAILATAPVML
                                          : :  . ::.:  * *  *: :.*  **. *: :.::   *:::

Mflv_4755|M.gilvum_PYR-GCK          VVALAVKLSSKGPVFYRQERVGLDGRPFEMIKFRTMVDGADGMLGKVAAL
Mvan_1703|M.vanbaalenii_PYR-1       PVAIAIKLTSKGPVFYRQERIGLDGHPFEMIKFRTMVDGADKMVDKLAAH
MMAR_4503|M.marinum_M               AAAIAIKLTSKGPVCYRAEHIGINGQPFTMLKLRTMVVGADSQIDGLVSL
MUL_4674|M.ulcerans_Agy99           AAAVAIKLTSKGPVCYRAERIGINGQPFTMLKLRTMVVGADSQIDHLVSR
MSMEG_5984|M.smegmatis_MC2_155      ITAIAIKATSRGPVFYSAERIGVDGKLFSMLKFRTMVENADQMLDELEDL
MAB_1020|M.abscessus_ATCC_1997      AIAAAIKLTSTGPVFYVSERIGLDGVPFRMIKFRSMYTGADLQTAGMIDS
                                      * *:* :* *** *  *::*::*  * *:*:*:*  .**     :   

Mflv_4755|M.gilvum_PYR-GCK          NESEGGVLFKIREDPRVTPVGKFLRKYSVDELPQFVNVLKRDMSVVGPRP
Mvan_1703|M.vanbaalenii_PYR-1       NESEGGVLFKIREDPRVTRVGKLLRKYSIDELPQFINVLKRDMSVVGPRP
MMAR_4503|M.marinum_M               NEGAGGILFKMRDDPRVTRIGRILRRFSIDEIPQFINVVKRDMSVVGPRP
MUL_4674|M.ulcerans_Agy99           NEGAGGILFKMRDDPRVTPIGRILRRFSVDEIPQFINVVKRDMSVVGPLP
MSMEG_5984|M.smegmatis_MC2_155      NE-SDGLLFKIHDDPRVTRVGKVLRRLSIDELPQFINVLRGEMSVVGPRP
MAB_1020|M.abscessus_ATCC_1997      AG-STPVFFKAKEDPRVTPVGAFIRKYSLDELPQFFNVLFGDMSVVGPRP
                                          ::** ::***** :* .:*: *:**:***.**:  :****** *

Mflv_4755|M.gilvum_PYR-GCK          PLATEVNSYDDYARRRLLVRPGITGLWQVSGRSDLSWEDSVRLDMFYVEN
Mvan_1703|M.vanbaalenii_PYR-1       PLAREVKSYDDYARRRLLVRPGITGLWQVSGRSDLSWEDSVRLDLFYVEN
MMAR_4503|M.marinum_M               PLSREVDSYDGDVRRRLLVKPGVTGIWQVSGRSQLSWEESVRLDLSYVDN
MUL_4674|M.ulcerans_Agy99           PLSREVDSYDGDVRRRLLVKPGVTGIWQVSGRSQLSWEESVRLDLSYVDN
MSMEG_5984|M.smegmatis_MC2_155      PLRREVEEYDCEILRRLLVKPGVTGLWQVSGRSDLSWDQAVRLDLSYVDN
MAB_1020|M.abscessus_ATCC_1997      QVQREVDAYDDTMRRRLLVKPGVTGQWQVSGRNDLSVDESIRHDISYVDN
                                     :  **. **    *****:**:** ******.:** ::::* *: **:*

Mflv_4755|M.gilvum_PYR-GCK          WSMVSDLLIALKTVKAMLGHSGAY
Mvan_1703|M.vanbaalenii_PYR-1       WSMIADLLIAVKTVKAVFSHSGAY
MMAR_4503|M.marinum_M               WSMAGDLMIIAKTVKAVLASRGAY
MUL_4674|M.ulcerans_Agy99           WSMTGGLMIIAKTVKAVLASRGAY
MSMEG_5984|M.smegmatis_MC2_155      WSMIGDILIVAKTFGAVLRKDGAY
MAB_1020|M.abscessus_ATCC_1997      WSMGLDIRIISRTVGAVVRSEGAY
                                    ***  .: *  :*. *:.   ***